{"id":25501,"date":"2020-06-19T15:55:02","date_gmt":"2020-06-19T07:55:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25501"},"modified":"2023-12-23T16:50:11","modified_gmt":"2023-12-23T08:50:11","slug":"senescence%e8%a1%b0%e8%80%81%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-facs-%e8%b2%a8%e8%99%9fk991-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25501","title":{"rendered":"Senescence\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2 (FACS) | Fluorescence\u8ca8\u865fK991-100 (ABCAM, ab228562)"},"content":{"rendered":"

\u00a0<\/strong>Home<\/strong><\/a> \u00a0 \u00a0<\/strong>Literature<\/strong><\/a>\u00a0 \u00a0<\/strong>Promotions<\/strong><\/a>\u00a0\u00a0<\/strong>Product Citations<\/strong><\/a><\/p>\n

Senescence<\/strong>\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2 (FACS)<\/strong> | <\/strong>\u8ca8\u865f<\/strong>K991-100 (ABCAM, ab228562)<\/a><\/span><\/p>\n

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\u00a0<\/em><\/strong>\u7522\u54c1\u63cf\u8ff0:<\/strong> \u8870\u8001(Senescence) \u88ab\u8a8d\u70ba\u662f\u816b\u7624\u6291\u5236\u6a5f\u5236(tumor suppressive mechanism)\u548c\u8870\u8001(aging)\u7684\u6839\u672c\u539f\u56e0\u3002\u8870\u8001(aging)\u4ee3\u8868\u4e86\u4e00\u7a2e\u505c\u6eef\u72c0\u614b\uff0c\u5176\u4e2d\u7d30\u80de\u4fdd\u6301\u6d3b\u529b\uff0c\u4f46\u6c92\u6709\u88ab\u8840\u6e05\u6216\u57f9\u990a\u7269\u523a\u6fc0\u5206\u88c2(divide)\u3002\u8870\u8001\u7d30\u80de(Senescent cells)\u986f\u793a\u7d30\u80de\u5927\u5c0f\u589e\u52a0\uff0c\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff08\u03b2-galactosidase , SA-\u03b2-Gal\uff09\u6d3b\u6027\u7684\u8870\u8001\u76f8\u95dc\u8868\u9054\u4ee5\u53ca\u57fa\u56e0\u8868\u9054\u6a21\u5f0f\u7684\u6539\u8b8a\u3002\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\u65e8\u5728\u901a\u904eFACS\u87a2\u5149\u6aa2\u6e2c\u57f9\u990a\u7d30\u80de\u4e2d\u7684SA-\u03b2-Gal\u6d3b\u6027\u3002 SA-\u03b2-Gal\u50c5\u5b58\u5728\u65bc\u8870\u8001\u7d30\u80de\u4e2d\uff0c\u800c\u672a\u5728\u8870\u8001\u524d\uff0c\u975c\u6b62\u6216\u6c38\u751f\u7d30\u80de\u4e2d\u767c\u73fe\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK991-100 (ABCAM, ab228562)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFACS (FL1 channel)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetection of senescence cells<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\nSimple, one step protocol<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Wash Buffer
\n\u2022 Senescence Dye<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Oil Red O Lipid Staining Kit, Catalog: COS-0101<\/td>\nCOS-0101<\/td>\nK580<\/td>\n<\/tr>\n
Senescence Staining Assay, Catalog: COS-0102<\/td>\nCOS-0102<\/td>\nK991<\/td>\n<\/tr>\n
Live vs. Dead Cell Differentiation Staining Kit, Catalog: COS-0103<\/td>\nCOS-0103<\/td>\nK501<\/td>\n<\/tr>\n
Beta-galactosidase Staining Kit, Catalog: COS-0104<\/td>\nCOS-0104<\/td>\nK802<\/td>\n<\/tr>\n
Alkaline Phosphatase Staining Kit, Catalog: COS-0105<\/td>\nCOS-0105<\/td>\nK2035<\/td>\n<\/tr>\n
LDH Based Cell Cytotoxicity Assay, Catalog: CPT-0101<\/td>\nCPT-0101<\/td>\nK313<\/td>\n<\/tr>\n
Crystal Violet Cell Cytotoxicity Assay, Catalog: CPT-0102<\/td>\nCPT-0102<\/td>\nK329<\/td>\n<\/tr>\n
MTT Cell Proliferation Assay, Catalog: CPT-0103<\/td>\nCPT-0103<\/td>\nK299<\/td>\n<\/tr>\n
MTS Cell Proliferation Assay, Catalog: CPT-0104<\/td>\nCPT-0104<\/td>\nK300<\/td>\n<\/tr>\n
Hydroxyproline Assay, Catalog: MA-0101<\/td>\nMA-0101<\/td>\nK226<\/td>\n<\/tr>\n
Lipid Peroxidation Assay, Catalog: MA-0102<\/td>\nMA-0102<\/td>\nK739<\/td>\n<\/tr>\n
Iron Assay, Catalog: MA-0103<\/td>\nMA-0103<\/td>\nK390<\/td>\n<\/tr>\n
Calcium Assay, Catalog: MA-0104<\/td>\nMA-0104<\/td>\nK380<\/td>\n<\/tr>\n
Phosphate Assay, Catalog: MA-0105<\/td>\nMA-0105<\/td>\nK410<\/td>\n<\/tr>\n
Total Antioxidant Capacity Assay, Catalog: MA-0106<\/td>\nMA-0106<\/td>\nK274<\/td>\n<\/tr>\n
Pyruvate Kinase Activity Assay, Catalog: MA-0107<\/td>\nMA-0107<\/td>\nK709<\/td>\n<\/tr>\n
Superoxide Dismutase Assay, Catalog: MA-0108<\/td>\nMA-0108<\/td>\nK335<\/td>\n<\/tr>\n
Alkaline Phosphatase Activity Assay Catalog: MA-0109<\/td>\nMA-0109<\/td>\nK412<\/td>\n<\/tr>\n
Triglyceride Assay, Catalog: MA-0110<\/td>\nMA-0110<\/td>\nK622<\/td>\n<\/tr>\n
Free Fatty Acid Assay, Catalog: MA-0111<\/td>\nMA-0111<\/td>\nK612<\/td>\n<\/tr>\n
Glycogen Assay, Catalog: MA-0112<\/td>\nMA-0112<\/td>\nK646<\/td>\n<\/tr>\n
ATP Assay, Catalog: MA-0113<\/td>\nMA-0113<\/td>\nK354<\/td>\n<\/tr>\n
NAD\/NADH Assay, Catalog: MA-0114<\/td>\nMA-0114<\/td>\nK337<\/td>\n<\/tr>\n
Lactate Assay, Catalog: MA-0115<\/td>\nMA-0115<\/td>\nK627<\/td>\n<\/tr>\n
Alanine Aminotransferase (ALT) Assay, Catalog: MA-0116<\/td>\nMA-0116<\/td>\nK752<\/td>\n<\/tr>\n
LDH Activity Assay, Catalog: MA-0117<\/td>\nMA-0117<\/td>\nK726<\/td>\n<\/tr>\n
Urea Assay, Catalog: MA-0118<\/td>\nMA-0118<\/td>\nK375<\/td>\n<\/tr>\n
Cholesterol-Cholesterol Ester Assay, Catalog: MA-0119<\/td>\nMA-0119<\/td>\nK603<\/td>\n<\/tr>\n
Aspartate Aminotransferase Activity Assay, Catalog: MA-0120<\/td>\nMA-0120<\/td>\nK753<\/td>\n<\/tr>\n
Creatinine Assay, Catalog: MA-0121<\/td>\nMA-0121<\/td>\nK625<\/td>\n<\/tr>\n
Glucose Assay, Catalog: MA-0122<\/td>\nMA-0122<\/td>\nK606<\/td>\n<\/tr>\n
L-Lactate Assay II, Catalog: MA-0123<\/td>\nMA-0123<\/td>\nK627<\/td>\n<\/tr>\n
HDL-LDL Cholesterol Assay, Catalog: MA-0124<\/td>\nMA-0124<\/td>\nK613<\/td>\n<\/tr>\n
Ammonia Assay, Catalog: MA-0125<\/td>\nMA-0125<\/td>\nK370<\/td>\n<\/tr>\n
NADP-NADPH Assay, Catalog: MA-0126<\/td>\nMA-0126<\/td>\nK347<\/td>\n<\/tr>\n
Acetyl CoA Assay, Catalog: MA-0127<\/td>\nMA-0127<\/td>\nK317<\/td>\n<\/tr>\n
Trypsin Activity Assay, Catalog: MA-0128<\/td>\nMA-0128<\/td>\nK771<\/td>\n<\/tr>\n
Pyruvate Assay, Catalog: MA-0129<\/td>\nMA-0129<\/td>\nK609<\/td>\n<\/tr>\n
Acetate Assay, Catalog: MA-0130<\/td>\nMA-0130<\/td>\nK658<\/td>\n<\/tr>\n
Ethanol Assay, Catalog: MA-0131<\/td>\nMA-0131<\/td>\nK620<\/td>\n<\/tr>\n
Beta-hydroxybutyrate Assay, Catalog: MA-0132<\/td>\nMA-0132<\/td>\nK632<\/td>\n<\/tr>\n
Glutamate Assay, Catalog: MA-0133<\/td>\nMA-0133<\/td>\nK629<\/td>\n<\/tr>\n
Succinate Assay, Catalog: MA-0134<\/td>\nMA-0134<\/td>\nK649<\/td>\n<\/tr>\n
Myeloperoxidase Activity Assay Catalog: MA-0135<\/td>\nMA-0135<\/td>\nK744<\/td>\n<\/tr>\n
Glycogen Assay II, Catalog: MA-0136<\/td>\nMA-0136<\/td>\nK648<\/td>\n<\/tr>\n
Glutathione Peroxidase Activity Assay, Catalog: MA-0137<\/td>\nMA-0137<\/td>\nK762<\/td>\n<\/tr>\n
\u03b1-Amylase Activity Assay, Catalog: MA-0138<\/td>\nMA-0138<\/td>\nK711<\/td>\n<\/tr>\n
Citrate Assay, Catalog: MA-0139<\/td>\nMA-0139<\/td>\nK655<\/td>\n<\/tr>\n
Ammonia Assay II, Catalog: MA-0140<\/td>\nMA-0140<\/td>\nK370<\/td>\n<\/tr>\n
\u03b1-Ketoglutarate Assay, Catalog: MA-0141<\/td>\nMA-0141<\/td>\nK677<\/td>\n<\/tr>\n
\u03b1-Galactosidase Activity Assay, Catalog: MA-0142<\/td>\nMA-0142<\/td>\nK407<\/td>\n<\/tr>\n
Catalase Activity Assay, Catalog: MA-0143<\/td>\nMA-0143<\/td>\nK773<\/td>\n<\/tr>\n
Total Carbohydrate Assay, Catalog: MA-0144<\/td>\nMA-0144<\/td>\nK645<\/td>\n<\/tr>\n
Hydrogen Peroxide Assay, Catalog: MA-0145<\/td>\nMA-0145<\/td>\nK265<\/td>\n<\/tr>\n
p300 Inhibitor Screening Assay, Catalog: MA-0146<\/td>\nMA-0146<\/td>\nK346<\/td>\n<\/tr>\n
Phenylalanine Assay, Catalog: MA-0147<\/td>\nMA-0147<\/td>\nK481<\/td>\n<\/tr>\n
D-Lactate Assay, Catalog: MA-0148<\/td>\nMA-0148<\/td>\nK667<\/td>\n<\/tr>\n
Lipase Activity Assay I, Catalog: MA-0149<\/td>\nMA-0149<\/td>\nK722<\/td>\n<\/tr>\n
Protein Carbonyl Assay, Catalog: MA-0150<\/td>\nMA-0150<\/td>\nK830<\/td>\n<\/tr>\n
Uric Acid Assay, Catalog: MA-0151<\/td>\nMA-0151<\/td>\nK608<\/td>\n<\/tr>\n
Choline\/Acetylcholine Assay, Catalog: MA-0152<\/td>\nMA-0152<\/td>\nK615<\/td>\n<\/tr>\n
GAPDH Activity Assay, Catalog: MA-0153<\/td>\nMA-0153<\/td>\nK680<\/td>\n<\/tr>\n
Cathepsin D-E Activity Assay, Catalog: MA-0154<\/td>\nMA-0154<\/td>\nK143<\/td>\n<\/tr>\n
Neutrophil Elastase Activity Assay (fluorometric), Catalog: MA-0156<\/td>\nMA-0156<\/td>\nK383<\/td>\n<\/tr>\n
Branched Chain Amino Acid Assay, Catalog: MA-0157<\/td>\nMA-0157<\/td>\nK564<\/td>\n<\/tr>\n
Angiotensin II Converting Enzyme Activity Assay, Catalog: MA-0158<\/td>\nMA-0158<\/td>\nK897<\/td>\n<\/tr>\n
L-Asparaginase Activity Assay, Catalog: MA-0159<\/td>\nMA-0159<\/td>\nK754<\/td>\n<\/tr>\n
Phosphodiesterase Activity Assay, Catalog: MA-0160<\/td>\nMA-0160<\/td>\nK927<\/td>\n<\/tr>\n
L-Carnitine Assay, Catalog: MA-0161<\/td>\nMA-0161<\/td>\nK642<\/td>\n<\/tr>\n
Alcohol Dehydrogenase Activity Assay, Catalog: MA-0162<\/td>\nMA-0162<\/td>\nK787<\/td>\n<\/tr>\n
Sulfatase Activity Assay, Catalog: MA-0163<\/td>\nMA-0163<\/td>\nK675<\/td>\n<\/tr>\n
Glutamine Assay, Catalog: MA-0164<\/td>\nMA-0164<\/td>\nK556<\/td>\n<\/tr>\n
Phosphofructokinase Activity Assay, Catalog: MA-0165<\/td>\nMA-0165<\/td>\nK776<\/td>\n<\/tr>\n
Pyruvate Dehydrogenase Activity Assay, Catalog: MA-0166<\/td>\nMA-0166<\/td>\nK679<\/td>\n<\/tr>\n
Cholesterol Detection Kit (cell-based), Catalog: MA-0167<\/td>\nMA-0167<\/td>\nK587<\/td>\n<\/tr>\n
Cathepsin B Activity Assay Kit (Fluorometric), Catalog: MA-0168<\/td>\nMA-0168<\/td>\nK140<\/td>\n<\/tr>\n
Aldehyde Dehydrogenase Assay, Catalog: MA-0169<\/td>\nMA-0169<\/td>\nK731<\/td>\n<\/tr>\n
Nickel Assa79:131y, Catalog: MA-0170<\/td>\nMA-0170<\/td>\nK510<\/td>\n<\/tr>\n
Lipolysis Assay, Catalog: MA-0189<\/td>\nMA-0189<\/td>\nK577<\/td>\n<\/tr>\n
Hemin Assay, Catalog: MA-0190<\/td>\nMA-0190<\/td>\nK672<\/td>\n<\/tr>\n
Elastin Assay Kit, Catalog: MA-0192<\/td>\nMA-0192<\/td>\nK2077<\/td>\n<\/tr>\n
Lipid Extraction Kit, Catalog: PI-0101<\/td>\nPI-0101<\/td>\nK216<\/td>\n<\/tr>\n
Sample Deproteinizing Kit (TCA), Catalog: PI-0102<\/td>\nPI-0102<\/td>\nK823<\/td>\n<\/tr>\n
Sample Deproteinizing Kit (PCA), Catalog: PI-0103<\/td>\nPI-0103<\/td>\nK808<\/td>\n<\/tr>\n
Plasma Membrane Isolation Kit, Catalog: PI-0104<\/td>\nPI-0104<\/td>\nK268<\/td>\n<\/tr>\n
Mitochondrial DNA Isolation Kit, Catalog: PI-0105<\/td>\nPI-0105<\/td>\nK280<\/td>\n<\/tr>\n
CytoFrac Cell Fractionation Kit, Catalog: PI-0106<\/td>\nPI-0106<\/td>\nK270<\/td>\n<\/tr>\n
Nuclear\/Cytosol Fractionation Kit, Catalog: PI-0107<\/td>\nPI-0107<\/td>\nK266<\/td>\n<\/tr>\n
Lysosome Isolation Kit, Catalog: PI-0108<\/td>\nPI-0108<\/td>\nK235<\/td>\n<\/tr>\n
Protease Inhibitor Cocktail, Catalog: PIC-0100<\/td>\nPIC-0100<\/td>\nK271<\/td>\n<\/tr>\n
96-Well Clear Plates, Catalog: M-0101<\/td>\nM-0101<\/td>\nM1360 \/ 1996<\/td>\n<\/tr>\n
96-Well White Plates, Catalog: M-0102<\/td>\nM-0102<\/td>\nM1354<\/td>\n<\/tr>\n
96-Well Black Plates, Catalog: M-0103<\/td>\nM-0103<\/td>\nM1355<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0Home \u00a0 \u00a0Literature\u00a0 \u00a0Promotions\u00a0\u00a0Product Citations Sen […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5380,3],"tags":[1464],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25501"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25501"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25501\/revisions"}],"predecessor-version":[{"id":47929,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25501\/revisions\/47929"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25501"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25501"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25501"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}