{"id":25490,"date":"2020-06-19T13:04:19","date_gmt":"2020-06-19T05:04:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25490"},"modified":"2026-03-30T15:21:02","modified_gmt":"2026-03-30T07:21:02","slug":"calcein-am%e9%88%a3%e9%bb%83%e7%b6%a0%e7%b4%a0%e6%b4%bb%e7%b4%b0%e8%83%9e%e8%9e%a2%e5%85%89%e6%9f%93%e5%8a%91-cas148504-34-1%e8%b2%a8%e8%99%9fbmd00064-50ug","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25490","title":{"rendered":"Calcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291 | CAS#148504-34-1\u8ca8\u865fBMD0064"},"content":{"rendered":"

Calcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291 | CAS#148504-34-1\u8ca8\u865fBMD0064<\/span><\/a>\u00a0<\/span><\/span><\/strong> \u00a0\u64cd\u4f5c\u624b\u518a<\/a>\u00a0\u00a0<\/span><\/span><\/p>\n

\"\"<\/p>\n

\u9223\u9ec3\u7da0\u7d20AM(Calcein AM) \u662f\u4e00\u7a2e\u7d30\u80de\u53ef\u6ef2\u900f(cell-permeable)\u7684\u67d3\u6599\uff0c\u5e38\u7528\u65bc\u6d3b\u7d30\u80de\u87a2\u5149\u6a19\u8a18\uff0cCalcein(\u9223\u9ec3\u7da0\u7d20)\u7684\u57fa\u790e\u4e0a\u52a0\u5f37\u4e86\u758f\u6c34\u6027\uff0c\u56e0\u6b64\u80fd\u5920\u8f15\u6613\u7a7f\u900f\u6d3b\u7d30\u80de\u819c\u3002\u7576\u5176\u9032\u5165\u5230\u6d3b\u7d30\u80de(live cells)\u5f8c\uff0c\u6703\u88ab\u7d30\u80de\u5167\u916f\u9176(intracellular esterases)\u88c2\u89e3\uff0c\u7559\u4e0b\u819c\u4e0d\u6ef2\u900f(membrane-impermeant)\u7684\u9223\u9ec3\u7da0\u7d20(Calcein)\uff0cCalcein\u9019\u662f\u4e00\u7a2e\u87a2\u5149\u6307\u793a\u5291\uff0c\u767c\u51fa\u5f37\u7da0\u8272\u87a2\u5149\u3002\u5176\u6700\u5927\u5438\u6536\u548c\u767c\u5c04\u6ce2\u9577\u5206\u5225\u70ba494\u548c517 nm\u3002\u5b83\u53ef\u7528\u4f5c\u7d30\u80de\u6d3b\u529b(cell viability)\uff0c\u7d30\u80de\u9593\u901a\u8a0a(cell-cell communication)\uff0c\u7d30\u80de\u6bd2\u6027(cytotoxicity)\u6216\u7d30\u80de\u5167\u9223(calcium)\uff0c\u6c1f(fluoride)\uff0c\u9435(iron)\u6216\u6c5e(mercury)\u8b8a\u5316\u7684\u6307\u6a19\u3002\u7531\u65bc\u9223\u9ec3\u7da0\u7d20\u662f\u591a\u85e5\u6297\u6027\u76f8\u95dc\u86cb\u767d1\uff08multidrug resistance-associated protein 1, MRP1\uff09\u548c\u591a\u85e5\u6297\u6027\u86cb\u767d3\uff08multidrug resistance protein 3, MDR3\uff0cP-\u7cd6\u86cb\u767d3\uff09\u7684\u5e95\u7269\uff0c\u56e0\u6b64\u9223\u9ec3\u7da0\u7d20AM(Calcein AM)\u7528\u65bc\u7814\u7a76\u9019\u4e9b\u86cb\u767d\u53ca\u5176\u8abf\u7bc0\u3002<\/p>\n

<\/p>\n

<\/p>\n

\u5f15\u7528\u6587\u737b<\/a><\/p>\n

<\/p>\n

\u5f15\u7528\u6587\u737b<\/a><\/p>\n

Calcein AM\u00a0\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291\u00a0|\u00a0\u7528\u65bc\u7d30\u80de\u6d3b\u6027\u5206\u6790\u4e4b\u5efa\u8b70\u914d\u88fd<\/span><\/p>\n

Calcein AM\u5206\u5b50\u91cf\u70ba995 g\/mole , \u7a31\u53d650ug\u7684calcein AM\u53ef\u4ee5\u6eb6\u572850ul\u7684DMSO \uff1b\u6700\u5f8c\u6fc3\u5ea6\u70ba1Mm\u53d61ul\u7684Calcein AM \u6eb6\u57281X \u76841000ul dissociation solution =1uM (1000ul)\u4ee596well \u70ba\u4f8b\uff0c\u52a0\u5165100ul\u76841uM Calcein AM,\u5927\u7d04\u53ef\u4ee5\u4f7f\u7528500\u6b21\u3002Calcein-AM\u7684ester\u90e8\u4f4d\u9047\u5230\u6fd5\u6c23\u6703\u5206\u89e3\uff0c\u4f7f\u7528\u5f8c\u8acb\u5728-20\u2103\u4e0b\u5bc6\u9589\u4fdd\u5b58\uff0c\u6eb6\u65bcDMSO\u5f8c\u5efa\u8b702\u500b\u6708\u5167\u4f7f\u7528\u5b8c\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCalcein AM<\/a><\/td>\n<\/tr>\n
CAS Number<\/strong><\/td>\n148504-34-1<\/td>\n<\/tr>\n
Molecular Formula<\/strong><\/td>\nC46<\/sub>H46<\/sub>N2<\/sub>O23<\/sub><\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nCalcein AM,BMD00064<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nGray solid<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 \u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/td>\n<\/tr>\n
Molecular weight<\/strong><\/td>\n995<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Soluble in DMSO<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStore at -20\u00b0C. The product can be stored for up to 12 months. Protect from light.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44 <\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
KTC4001<\/td>\nTraKine\u2122 Cell Plasma Membrane Staining Kit (Green Fluorescence)<\/td>\n<\/tr>\n
KTC4002<\/td>\nTraKine\u2122 Cell Plasma Membrane Staining Kit (Orange Fluorescence)<\/td>\n<\/tr>\n
KTC4003<\/td>\nTraKine\u2122 Mitochondrion Staining Kit (Green Fluorescence)<\/td>\n<\/tr>\n
KTC4004<\/td>\nTraKine\u2122 Mitochondrion Staining Kit (Orange Fluorescence)<\/td>\n<\/tr>\n
KTC4005<\/td>\nTraKine\u2122 Mitochondrion and Nuclear Staining Kit<\/td>\n<\/tr>\n
KTC4008<\/td>\nTraKine\u2122 F-actin Staining Kit (Green Fluorescence)<\/td>\n<\/tr>\n
KTC4009<\/td>\nTraKine\u2122 F-actin Staining Kit (Orange Fluorescence)<\/td>\n<\/tr>\n
KTC4100<\/td>\nTraKine\u2122 Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution)<\/td>\n<\/tr>\n
BMD0050<\/td>\nCy3<\/td>\n<\/tr>\n
BMD0051<\/td>\nCy5<\/td>\n<\/tr>\n
BMD0060<\/td>\nEthidium Homodimer-1 (EthD-1)<\/td>\n<\/tr>\n
BMD0061<\/td>\nHoechst 33258<\/td>\n<\/tr>\n
BMD0062<\/td>\nHoechst 33342<\/td>\n<\/tr>\n
BMD0063<\/td>\nDAPI<\/td>\n<\/tr>\n
BMD0064<\/td>\nCalcein AM<\/td>\n<\/tr>\n
BMD0066<\/td>\n5(6)-CFDA, SE<\/td>\n<\/tr>\n
BMD0071<\/td>\nDiI (DiIC18(3))<\/td>\n<\/tr>\n
BMD0072<\/td>\nDiO (DiOC18(3))<\/td>\n<\/tr>\n
BMD0073<\/td>\nDiD (DiIC18(5))<\/td>\n<\/tr>\n
BMD0074<\/td>\nDiR (DiIC18(7))<\/td>\n<\/tr>\n
BMD0082<\/td>\nAbFluor\u2122 488-Phalloidin<\/td>\n<\/tr>\n
BMD0083<\/td>\nAbFluor\u2122 555-Phalloidin<\/td>\n<\/tr>\n
BMD0084<\/td>\nAbFluor\u2122 594-Phalloidin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n\u7522\u54c1\u8ca8\u865f<\/td>\n\u53cd\u61c9<\/td>\n\u6e2c\u91cf\u5100\u5668<\/td>\n<\/tr>\n
\u00a0\u8ca8\u865fKTA1001<\/a><\/td>\n\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\n

\u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/p>\n

\u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/td>\n<\/tr>\n

\n

\u8ca8\u865f\u00a0KTA2010<\/a><\/p>\n

 <\/td>\n

\n

TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/p>\n

 <\/td>\n

50\u500b\u53cd\u61c9<\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/td>\n<\/tr>\n
\n

\u8ca8\u865f\u00a0KTC011001<\/a><\/p>\n

 <\/td>\n

CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/a><\/td>\n500\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx\/Em = 500\/520 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em = 484\/501 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n10MG\/100MG<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA3030<\/a><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1020<\/a><\/td>\nCCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n1000\u500b\u53cd\u61c9\/10,000\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTA2030<\/a><\/td>\n\n

\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/p>\n

(BrdU\u66ff\u4ee3\u6cd5)<\/td>\n

100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/a><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (540 nm)<\/p>\n

\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/td>\n<\/tr>\n

\u8ca8\u865fKTD3001<\/a><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/td>\n\n

2000\u500b\u53cd\u61c9\/<\/p>\n

5000\u500b\u53cd\u61c9<\/td>\n

\n

OD 562 nm<\/p>\n

\u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/p>\n

50 to 1000 \u00b5g\/ml.<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1110<\/a><\/td>\n\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u03bbmax= 450 nm<\/p>\n

1- 20 U\/mL of LDH<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>PRP100153<\/a><\/td>\nIGF-I\u91cd\u7d44\u86cb\u767d<\/a><\/td>\n200UG\/1MG<\/td>\n\n

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)<\/p>\n

> 95 % as determined by SDS-PAGE<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTA2020<\/a><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em=535\/615 nm.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMC21400<\/a><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u9810\u88dd\u96e2\u5fc3\u67f1<\/a><\/td>\n\n

1ml\u00d75<\/p>\n

3mlx5<\/td>\n

More than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1200<\/a><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149370 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1080<\/a><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1090<\/a><\/td>\n\u6e05\u9664\u6c2b\u6c27\u81ea\u7531\u57fa\u80fd\u529b\u6e2c\u5b9a\u8a66\u5291<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149 536nm Hydroxyl radical scavenging rate D% =\uff08ASample – AControl\uff09\u00f7\uff08ABlank – AControl\uff09\u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB2220<\/a><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

500nm Standard: 5 \u03bcmol\/mL<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1510<\/a><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u6536\u5149505 nm<\/p>\n

Standard: 5 \u03bcmol\/mL.<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1300<\/a><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u5149\u503c630nm<\/p>\n

4- 300 mg\/dL)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1100<\/a><\/td>\nLactate\u00a0<\/u><\/strong><\/a> \u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (450 nm)<\/p>\n

0.03 mM-2 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1700<\/a><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\u5438\u6536\u5149510 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1500<\/a><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTAC\u5206\u6790<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

593 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTA0002<\/a><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n20\u500b\u53cd\u61c9\/50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KET7013<\/a><\/td>\nEliKine\u2122 Rat IL-6 ELISA Kit<\/u><\/strong>\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET0003<\/a><\/td>\n\u96cc\u4e8c\u9187\uff08Estradiol , E2\uff09\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d40 pg\/ml-1500 pg\/ml<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6032<\/a><\/td>\nTNF<\/u><\/em>–\u03b1<\/u><\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6033<\/a><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET7005<\/a><\/td>\n\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTE70003<\/a><\/td>\n\u5c0f\u9f20\u9ec3\u560c\u5464\u812b\u6c2b\u9176\/\u6c27\u5316\u9176\uff08XDH\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 22.5 ng\/L-360 ng\/L, \u6700\u4f4e\u9748\u654f\u5ea61.0 ng\/L\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f\u00a0A02051HRP<\/u><\/strong><\/a><\/td>\nHis-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0<\/a><\/td>\n50ul\/200ul<\/td>\nWB (1:5000).<\/td>\n<\/tr>\n
\u8ca8\u865fA02170MGB<\/a><\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5), \u78c1\u73e0<\/a><\/td>\n100ul\/400ul\/2ml<\/td>\nGKPIPNPLLGLDST epitope<\/td>\n<\/tr>\n
\u8ca8\u865fKTA4001<\/a><\/td>\nJC-1<\/u><\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

100\u500b\u53cd\u61c9\/<\/p>\n

500\u500b\u53cd\u61c9<\/td>\n

\n

\uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/p>\n

JC-1 Stain<\/p>\n

CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1600<\/a><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

412 nm<\/p>\n

2-200 \u00b5g\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n1MG\/10MG\/100MG<\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTP3001<\/a><\/td>\n\u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2<\/a><\/td>\n\n

50\u500b\u53cd\u61c9\/<\/p>\n

200\u500b\u53cd\u61c9<\/td>\n

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) \u2022 Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00064-50UG<\/a><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/td>\n50ug\/1mg\/10mg<\/td>\n\n

\u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTB1660<\/a><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n240nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1020<\/a><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD565 nm<\/p>\n

96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1040<\/a><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 540 nm<\/p>\n

2-75 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1060<\/a><\/td>\n\u904e\u6c27\u5316\u7269\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 664 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d\u70ba15.6-1000 \u00b5U\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1050<\/a><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 532 nm<\/p>\n

1-100 \u00b5M\/L<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1030<\/a><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n200-3.13 U\/ml<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1030<\/a><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0OD 565 nm<\/p>\n

10,00-100,0000 cells per well<\/td>\n<\/tr>\n

\u8ca8\u865fKTB3010<\/a><\/td>\nCheKine\u2122 \u82b1\u9752\u7d20\u9084\u539f\u9176\uff08ANR\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

absorption at 340 nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1542<\/a><\/td>\nTannase(TAN) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

absorbance at 270 nm<\/p>\n

0.3125-20 \u03bcmoL\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1541<\/a><\/td>\nCheKine\u2122 \u55ae\u5be7\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Tannin Colorimetric Assay Kit<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

absorbance at 760 nm<\/p>\n

0.0156-1 mg\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB2250<\/a><\/td>\nCheKine\u2122 \u5fae\u91cf\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (HDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

Absorption peak at 500nm<\/p>\n

Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol\/L\uff09<\/td>\n<\/tr>\n

\u8ca8\u865fKTB2260<\/a><\/td>\nCheKine\u2122 \u5fae\u91cf\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (LDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

Absorption peak at 500nm<\/p>\n

Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol\/L\uff09<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1290<\/a><\/td>\nCheKine\u2122 \u70cf\u982d\u9178\u9176 (ACO) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

absorption at 340 nm.<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1560<\/a><\/td>\n\u9187\u91af\u57fa\u8f49\u79fb\u9176\uff08AAT\uff09\u6d3b\u6027\u6bd4\u8272\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

absorption at 412 nm.<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1390<\/a><\/td>\nCheKine\u2122 \u6fb1\u7c89\u5206\u652f\u9176 (SBE) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

absorption at 660 nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u7522\u54c1\u540d\u7a31<\/td>\n\u80de\u5668\u67d3\u8272\u4f4d\u7f6e<\/td>\n\u87a2\u5149\u6ce2\u6bb5<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n\u7c92\u7dda\u9ad4(mitochondria)<\/td>\nEx \/ Em = 490\/523 nm<\/td>\n\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n\u5fae\u7ba1\u86cb\u767d(Tubulin)<\/td>\nEx\/Em = 500\/520 nm<\/td>\n\u6d3b\u7d30\u80de\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n\u7d30\u80de\u819c<\/a>\u00a0(plasma membrane)<\/td>\nEx\/Em = 484\/501 nm<\/td>\n\u6d3b\u7d30\u80de\u3001\u56fa\u5b9a\u61f8\u6d6e\u3001\u8cbc\u9644\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n\u7d30\u80de\u6838 dsDNA<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\nLysosome<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Calcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291 | CAS#148504-34-1\u8ca8\u865fBMD0064\u00a0 \u00a0\u64cd\u4f5c\u624b\u518a […]<\/p>\n","protected":false},"author":3,"featured_media":29551,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1466],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25490"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25490"}],"version-history":[{"count":8,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25490\/revisions"}],"predecessor-version":[{"id":52162,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25490\/revisions\/52162"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29551"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25490"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25490"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25490"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}