{"id":25377,"date":"2020-06-16T14:15:22","date_gmt":"2020-06-16T06:15:22","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25377"},"modified":"2023-12-12T15:19:10","modified_gmt":"2023-12-12T07:19:10","slug":"gsh-px%e7%a9%80%e8%83%b1%e7%94%98%e8%82%bd%e9%81%8e%e6%b0%a7%e5%8c%96%e7%89%a9%e9%85%b6%e6%aa%a2%e6%b8%ac-chekine-reduced-glutathione-colorimetric-assay-kit%e8%b2%a8%e8%99%9f-ktb1640","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25377","title":{"rendered":"GPx\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c |Glutathione Peroxidase (GPx\/GSH-Px) Activity Assay Kit\u8ca8\u865f KTB1640"},"content":{"rendered":"

GPx\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c |Glutathione Peroxidase (GPx\/GSH-Px) Activity Assay Kit\u8ca8\u865f KTB1640<\/a>\u00a0(340nm)<\/span><\/strong><\/span><\/p>\n

Glutathione peroxidase<\/span>\u00a0(GSH-Px)\uff0c\u7c21\u7a31\u70baGSH-Px\u6216GPx<\/p>\n

\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\u7a40\u80f1\u7518\u80bd (Glutathione) \u7814\u7a76\u7684\u610f\u7fa9<\/span><\/strong><\/span><\/p>\n

\u7a40\u80f1\u7518\u80bd\u662f\u542b\u6709\u8c37\u6c28\u9178 (glutamate)\uff0c\u534a\u80f1\u6c28\u9178(cysteine)\u548c\u7518\u6c28\u9178(glycine)\u7684\u4e09\u80bd\uff0c\u5176\u4e2d\u542b\u6709\u5df0\u57fa(sulfhydryl groups)\u3002\u5b83\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u690d\u7269\u4e2d\u3002\u5b83\u5177\u6709\u6297\u6c27\u5316\u5291(antioxidant)\u548c\u7d9c\u5408\u89e3\u6bd2(detoxification)\u4f5c\u7528\u3002\u5176\u76f8\u95dc\u7684\u8f14\u9176(coenzyme)\u662fNADPH\u3002\u4eba\u9ad4\u65b0\u9673\u4ee3\u8b1d\u7522\u751f\u7684\u81ea\u7531\u57fa(free radicals)\u904e\u591a\u6703\u7834\u58de\u751f\u7269\u819c(biofilm)\uff0c\u4fb5\u5165\u751f\u547d\u5927\u5206\u5b50\uff0c\u52a0\u901f\u4eba\u9ad4\u8870\u8001(aging)\uff0c\u4e26\u8a98\u767c\u816b\u7624(tumors)\u6216\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis) \u7684\u7522\u751f\u3002\u7a40\u80f1\u7518\u80bd\u5177\u6709\u589e\u5f37\u751f\u7269\u9ad4\u7684\u6297\u6c27\u5316\u529f\u80fd\uff0c\u4fc3\u9032\u751f\u9577\u4ee5\u53ca\u589e\u5f37\u751f\u7269\u9ad4\u5c0d\u4eba\u9ad4\u4e0d\u5229\u74b0\u5883\u7684\u5f71\u97ff\u7684\u529f\u80fd\u3002\u5b83\u7684\u4e3b\u8981\u751f\u7406\u4f5c\u7528\u662f\u6e05\u9664\u9ad4\u5167\u7684\u81ea\u7531\u57fa\u3002\u5b83\u4e5f\u662f\u4e00\u7a2e\u5f88\u597d\u7684\u89e3\u6bd2\u5291\uff0c\u53ef\u4ee5\u6e1b\u5f31\u6709\u6bd2\u7269\u8cea\u7684\u6bd2\u6027\u4e26\u5f9e\u9ad4\u5167\u6e05\u9664\u6709\u6bd2\u7269\u8cea\u3002\u7a40\u80f1\u7518\u80bd\u9084\u4fc3\u9032\u751f\u9577\u4e26\u63d0\u9ad8\u4eba\u9ad4\u514d\u75ab\u529b\u3002\u7a40\u80f1\u7518\u80bd(Glutathione)\u4e0d\u50c5\u53ef\u4ee5\u7528\u4f5c\u85e5\u7269\uff0c\u9084\u53ef\u4ee5\u7528\u4f5c\u529f\u80fd\u6027\u98df\u54c1\u7684\u57fa\u790e\u6750\u6599\uff0c\u4e26\u4e14\u5ee3\u6cdb\u7528\u65bc\u529f\u80fd\u6027\u98df\u54c1\u4e2d\uff0c\u4f8b\u5982\u6297\u8870\u8001(anti-aging)\uff0c\u589e\u5f37\u514d\u75ab\u529b(enhancing immunity)\u548c\u6297\u816b\u7624\u4f5c\u7528(anti-tumor)\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong><\/span><\/p>\n

GSH-Px \/GPx\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176,\u00a0Glutathione peroxidase<\/span>\u00a0(GSH-Px)\uff0c\u7c21\u7a31\u70baGSH-Px<\/b>\u6216GPx\u3002<\/b>\u662f\u5728\u7a40\u80f1\u7518\u80bd\u6c27\u5316\u9084\u539f\u5faa\u74b0\u4e2d\u50ac\u5316\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08reduced glutathione , GSH\uff09\u6c27\u5316\u7684\u4e3b\u8981\u9176\u4e4b\u4e00\u3002 GSH-Px \/GPx\u3010\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176,\u00a0Glutathione Peroxidase (GSH-Px)\u3011\u4e0d\u50c5\u80fd\u7279\u7570\u6027\u50ac\u5316\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u8207ROS\u4e4b\u9593\u7684\u53cd\u61c9\uff0c\u5f62\u6210\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bdGSSG\uff0c\u5f9e\u800c\u4fdd\u8b77\u751f\u7269\u819c\u514d\u53d7ROS\u7834\u58de\u4e26\u7dad\u6301\u7d30\u80de\u7684\u6b63\u5e38\u529f\u80fd\u3002\u5b83\u9084\u53ef\u4ee5\u4fdd\u8b77\u809d\u81df\uff0c\u63d0\u9ad8\u4eba\u9ad4\u514d\u75ab\u529b\uff0c\u5c0d\u6297\u6709\u5bb3\u91d1\u5c6c\u96e2\u5b50(metal ions)\u5c0d\u4eba\u9ad4\u7684\u6709\u5bb3\u5f71\u97ff\uff0c\u4e26\u589e\u5f37\u4eba\u9ad4\u62b5\u6297\u8f3b\u5c04(radiation)\u7684\u80fd\u529b\u3002GSH-Px \u3010\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176,\u00a0Glutathione Peroxidase (GSH-Px \/GPx)\u3011\u50ac\u5316H2O2\u6c27\u5316GSH\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08reduced glutathione , GSH\uff09\uff0c\u751f\u6210GSSG (\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bdGSSG)\u3002\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08glutathione reductase , GR\uff09\u50ac\u5316NADPH\u9084\u539fGSSG(\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bdGSSG)\uff0c\u5f9e\u800c\u518d\u751fGSH\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08reduced glutathione , GSH\uff09\uff0c\u800cNADPH\u6c27\u5316\u7522\u751fNADP +\u3002 NADPH\u5728340 nm\u8655\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u6c92\u6709\u3002\u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4f86\u78ba\u5b9aNADPH\u812b\u6c2b\u901f\u7387\uff0c\u4ee5\u8a08\u7b97GSH-Px \u3010\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176,\u00a0Glutathione Peroxidase (GSH-Px \/GPx)\u3011\u6d3b\u6027\u3002<\/p>\n

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\u6587\u737b\u5f15\u7528<\/a> Multispectroscopic, virtual and in vivo insights into the photoaging defense mediated by the natural food colorant bixin<\/p>\n

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(\u6587\u737b\u5f15\u7528<\/a>) Effects of salinity on GSH-Px and AKP activities in the liver tissue of P. fucata with two shell colors (I: 12h, GSH-Px activity; II: 24h, GSH-Px activity; III: 12h, AKP activity; IV: 24h, AKP activity).<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Glutathione Peroxidase (GSH-Px \/GPx) Activity Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nGSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Assay Buffer
\n\u2022 Substrate
\n\u2022 GR
\n\u2022 H2O2<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of GSH-Px activity in serum, plasma, plant tissue\/cell lysates and other biological fluids.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
\n\u2022 The whole processes need to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
\n\u2022 Prepare mixed reagents for use, and place on ice.
\n\u2022 The measuring process should be operated quickly and only 1 ~ 2 blank well should be needed.
\n\u2022 The reaction temperature has influence on the result. Keep the temperature at 25\u00b0C (for general species) or 37\u00b0C (for mammals).<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at -20\u00b0C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nGSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px not only specifically catalyzes the reaction between reduced glutathione and ROS to form oxidized glutathione GSSG, thereby protecting the biofilm from ROS damage and maintaining the normal function of cells; it also protects the liver and improves the body immunity, antagonizing the harmful effects of harmful metal ions on the body and increasing the body’s ability to resist radiation.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6aa2\u6e2c\u8102\u8cea\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6-\uff08SOD\u3001CAT\u3001GPx\u3001MDA\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/span><\/strong><\/span><\/h1>\n

\u5224\u65b7\u6a5f\u9ad4\u6216\u7d30\u80de\u7684\u8102\u8cea\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6\uff0c\u4e00\u65b9\u9762\u9700\u8981\u6aa2\u6e2c\u6297\u6c27\u5316\u9176\uff08SOD\u3001CAT\u3001GPx\uff09\u6d3b\u529b\u4ee5\u5224\u65b7\u85e5\u7269\u7b49\u6e05\u9664\u6c27\u81ea\u7531\u57fa\u7684\u80fd\u529b\uff0c\u53e6\u4e00\u65b9\u9762\u6aa2\u6e2c\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269MDA\u7684\u6c34\u5e73\u4ee5\u9593\u63a5\u5224\u65b7\u7d30\u80de\u53d7\u81ea\u7531\u57fa\u653b\u64ca\u7684\u56b4\u91cd\u7a0b\u5ea6\u3002\u540c\u6642\u5206\u6790SOD\u3001CAT\u3001GPx\u3001MDA\u7684\u6c34\u5e73\u7d50\u679c\u5206\u6790\u53ef\u66f4\u5168\u9762\u8a55\u4f30\u6c27\u5316\u58d3\u529b\u6c34\u5e73\u7684\u8b8a\u5316\u3002\u773e\u6240\u5468\u77e5\uff0c\u6c27\u5316\u58d3\u529b(oxidative stress)\u662f\u56e0\u70ba\u6a5f\u9ad4\u6d3b\u6027\u6c27\u6c34\u5e73\u548c\u5167\u6e90\u6027\u6297\u6c27\u5316\u80fd\u529b\u4e4b\u9593\u5931\u53bb\u4e86\u5e73\u8861\uff0c\u5c0e\u81f4\u7d30\u80de\u6297\u6c27\u5316\u80fd\u529b\u4e0d\u8db3\uff0c\u7d30\u80de\u5167\u6bd2\u6027\u6d3b\u6027\u6c27\uff08ROS\uff09\u589e\u52a0\uff0c\u7522\u751f\u6c27\u81ea\u7531\u57fa\uff08 O2-\uff0c.OH, H2O2\uff09\uff0c\u4f7f\u751f\u7269\u5927\u5206\u5b50\u53d7\u5230\u653b\u64ca\uff0c\u56e0\u800c\u9020\u6210\u7684\u7d30\u80de\u548c\u7d44\u7e54\u640d\u50b7\u3002<\/p>\n

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\u8102\u8cea\u6c27\u5316\u50b7\u5bb3<\/strong><\/h3>\n

\u8eab\u9ad4\u4e2d\u6c27\u5316\u9084\u539f\u72c0\u614b\u5931\u8861\u52e2\u5fc5\u6703\u9020\u6210\u56b4\u91cd\u5f8c\u679c\uff0c\u4e0d\u540c\u7d44\u7e54\u7d30\u80de\u7b49\u6703\u906d\u53d7\u5230\u4e0d\u540c\u7a0b\u5ea6\u7684\u640d\u50b7\u8207\u7834\u58de\uff0c\u4e3b\u8981\u8868\u73fe\u5728\u8102\u8cea\u3001\u80fa\u57fa\u9178\u548c\u86cb\u767d\u8cea\u7684\u6c27\u5316\u640d\u50b7\u3001\u6838\u9178\u548c\u67d3\u8272\u9ad4\u7b49\u6c27\u5316\u640d\u50b7\u65b9\u9762\u3002\u8102\u8cea\u8207ROS\uff08\u5c24\u5176\u662f\u2022 OH\uff09\u53cd\u61c9\u662f\u7d30\u80de\u6c27\u5316\u640d\u50b7\u7684\u666e\u904d\u6a5f\u5236\uff0c\u6b64\u53cd\u61c9\u4e3b\u8981\u53d6\u6c7a\u65bc\u7d30\u80de\u819c\u6d41\u52d5\u6027\u3002\u81ea\u7531\u57fa\u653b\u64ca\u4e0d\u98fd\u548c\u8102\u80aa\u9178\uff0c\u964d\u4f4e\u819c\u7684\u6d41\u52d5\u6027\uff0c\u5f71\u97ff\u7d30\u80de\u751f\u7406\u529f\u80fd\uff1b\u8102\u8cea\u904e\u6c27\u5316\u904e\u7a0b\u4e2d\u7522\u751f\u7684\u6c27\u81ea\u7531\u57fa\u53ef\u6c27\u5316\u4fee\u98fe\u7d30\u80de\u819c\u4e0a\u548c\u8cea\u5167\u7684\u9176\uff0c\u4f7f\u5176\u5931\u53bb\u6d3b\u6027\u3002\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269\uff0c\u5982\u4e19\u4e8c\u919b\uff08MDA\uff09\u80fd\u8207\u86cb\u767d\u3001\u6838\u9178\u53ca\u8166\u78f7\u8102\u7b49\u542b\u6c28\u57fa\u5316\u5408\u7269\u53cd\u61c9\uff0c\u4f7f\u4e4b\u767c\u751f\u4ea4\u806f\u3002\u8102\u8cea\u904e\u6c27\u5316\u53ef\u85c9\u7531\u7522\u751f\u7684\u81ea\u7531\u57fa\u640d\u50b7\u7d30\u80de\uff0c\u8a98\u5c0e\u7d30\u80de\u51cb\u4ea1\uff0c\u540c\u6642\u5176\u7d30\u80de\u6bd2\u6027\u53ef\u5f71\u97ff\u591a\u7a2e\u9175\u7d20\u7684\u6d3b\u6027\u53caATP\u7684\u5408\u6210\u3002<\/p>\n

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\u6b63\u5e38\u60c5\u6cc1\u4e0b\uff0c\u751f\u7269\u9ad4\u4e2d\u5929\u7136\u7684\u6297\u6c27\u5316\u5291\u9632\u79a6\u7cfb\u7d71\u4e2d\u7684\u6297\u6c27\u5316\u9176\u53ef\u4ee5\u8207\u98f2\u98df\u6216\u85e5\u7269\u4e2d\u7684\u6297\u6c27\u5316\u5291\u5354\u540c\u4f5c\u7528\uff0c\u6e05\u9664\u904e\u6c27\u5316\u7269\u3002\u5176\u4e2d\u6700\u91cd\u8981\u7684\u6297\u6c27\u5316\u9175\u7d20\u5305\u62ec\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\uff0c\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\uff08GPx\uff09\u548c\u904e\u6c27\u5316\u6c2b\u9176\uff08Cat\uff09\u3002SOD\u8ca0\u8cac\u5c07\u8d85\u6c27\u9670\u96e2\u5b50\u6b67\u5316\u70ba\u904e\u6c27\u5316\u6c2b\uff0c\u800cCat\u548cGPxs\u5247\u53ef\u4ee5\u6e1b\u5c11\u904e\u6c27\u5316\u6c2b\uff0c\u963b\u6b62\u9ad8\u6bd2\u6027\u7fa5\u57fa\u81ea\u7531\u57fa\u7684\u7522\u751f\u3002\u91cd\u8981\u7684\u662f\uff0cGPxs\u9084\u53ef\u4ee5\u6e1b\u5c11\u591a\u5143\u4e0d\u98fd\u548c\u8102\u80aa\u9178\u7684\u6c2b\u904e\u6c27\u5316\u7269\uff0c\u62b5\u6d88\u8102\u8cea\u904e\u6c27\u5316\u7684\u6bd2\u6027\u4f5c\u7528\u3002<\/p>\n

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\u540c\u6642\u56e0\u70ba\u8102\u8cea\u904e\u6c27\u5316\u662f\u7531\u81ea\u7531\u57fa\u555f\u52d5\u7684\uff0c\u4e0d\u98fd\u548c\u8102\u80aa\u9178\u5728\u81ea\u7531\u57fa\u548c\u4e00\u4e9b\u6c27\u5316\u5f15\u767c\u8102\u985e\u6c27\u5316\u7684\u6700\u521d\u968e\u6bb5\u5f62\u6210\u5171\u8edb\u4e8c\u70ef, \u7136\u5f8c\u5f62\u6210\u74b0\u72c0\u904e\u6c27\u5316\u7269, \u6700\u5f8c\u7684\u4e3b\u8981\u7522\u7269\u662f\u4e19\u4e8c\u919b\uff08MDA\uff09\u3002<\/p>\n

<\/p>\n

\u6240\u4ee5\uff0c\u5224\u65b7\u6a5f\u9ad4\u6216\u7d30\u80de\u7684\u8102\u8cea\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6\uff0c\u4e00\u65b9\u9762\u9700\u8981\u6aa2\u6e2c\u6297\u6c27\u5316\u9176\uff08SOD\u3001CAT\u3001GPx\uff09\u6d3b\u529b\u4ee5\u5224\u65b7\u85e5\u7269\u7b49\u6e05\u9664\u6c27\u81ea\u7531\u57fa\u7684\u80fd\u529b\uff0c\u53e6\u4e00\u65b9\u9762\u6aa2\u6e2c\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269MDA\u7684\u6c34\u5e73\u4ee5\u9593\u63a5\u5224\u65b7\u7d30\u80de\u53d7\u81ea\u7531\u57fa\u653b\u64ca\u7684\u56b4\u91cd\u7a0b\u5ea6\u3002\u540c\u6642\u5206\u6790SOD\u3001CAT\u3001GPx\u3001MDA\u7684\u6c34\u5e73\u7d50\u679c\u5206\u6790\u53ef\u66f4\u5168\u9762\u8a55\u4f30\u6c27\u5316\u58d3\u529b\u6c34\u5e73\u7684\u8b8a\u5316\u3002<\/p>\n

Abbkine \u53ef\u63d0\u4f9b\u5168\u5957\u8102\u8cea\u6c27\u5316\u640d\u50b7\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u6aa2\u6e2c\u65b9\u6cd5\u7c21\u55ae\u3001\u65b9\u4fbf\u3001\u9748\u654f\uff0c\u7522\u54c1\u8a73\u60c5\u5982\u4e0b\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n
\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u6aa2\u6e2c\u65b9\u6cd5<\/strong><\/td>\n\u9748\u654f\u5ea6<\/strong>&<\/strong>\u6aa2\u6e2c\u7bc4\u570d<\/strong><\/td>\n\u9069\u5408\u6a23\u672c<\/strong><\/td>\n\u8ca8\u865f<\/strong><\/td>\n<\/tr>\n
CheKine\u2122 \u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176(SOD) \u6d3b\u529b\u6aa2\u6e2c\u8a66\u5291\u76d2<\/td>\n\u8f03\u7a69\u5b9a\/\u9748\u654f\u7684WST-8\u986f\u8272\u6cd5<\/td>\n3.13U\/ml -200U\/ml<\/td>\n\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2<\/td>\nKTB1030<\/a><\/td>\n<\/tr>\n
\n

CheKine\u2122 Catalase (CAT)<\/p>\n

\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/td>\n

\u76f4\u63a5\u5229\u7528\u904e\u6c27\u5316\u6c2b\u9176\u7684\u904e\u6c27\u5316\u529f\u80fd\u76f4\u63a5\u6e2c\u5b9a\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027<\/td>\n\u9748\u654f\u5ea6\u4f4e\u81f32 U\/ml<\/td>\n\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d05\u8840\u7403\u88c2\u89e3\u7522\u7269\u3001\u7d44\u7e54\u52fb\u6f3f\u3001\u7d30\u80de\u88c2\u89e3\u6db2\u7b49\u751f\u7269\u6a23\u54c1<\/td>\nKTB1040<\/a><\/td>\n<\/tr>\n
CheKine\u2122\u8102\u8cea\u904e\u6c27\u5316\uff08MDA\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2<\/td>\n\u6539\u826f\u7684\u786b\u4ee3\u5df4\u6bd4\u59a5\u9178(TBA)\u6cd5<\/td>\n1-100 \u00b5M\/L<\/td>\n\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u5c3f\u6db2\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2<\/td>\nKTB1050<\/a><\/td>\n<\/tr>\n
\n

CheKine\u2122\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\uff08GSH-Px\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2<\/p>\n

 <\/td>\n

\u76f4\u63a5\u5229\u7528GSH-Px\u9176\u50ac\u5316H\u00a02<\/sub>\u00a0O\u00a02<\/sub>\u7684\u6d3b\u6027\u9032\u884c\u6aa2\u6e2c<\/td>\n\/\/<\/td>\n\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2<\/td>\nKTB1640<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u767c\u8868\u7684\u6587\u737b\uff1a<\/strong><\/span><\/p>\n

Anton Bahtiar*, Putri Sagita Utami\uff0cetc. The Antioxidant Effects of the Ethanolic Extract of Binahong Leaves Unilateral Ureteral Obstruction Rat Model\uff0cPharmacogn J. DOI : 10.5530\/pj.2021.13.26.<\/p>\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/strong><\/td>\nCheKine\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u00a0 \u00a0 \u00a0 \u00a0CheKine<\/strong>\u7522\u54c1\u539f\u7406<\/strong>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/strong><\/td>\n<\/tr>\n
KTB1600<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31: CheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit<\/p>\n

\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6aa2\u6e2c<\/a><\/span><\/td>\n

\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2 (KTB1600)\u5229\u7528DTNB\u8207\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53cd\u61c9\u5f62\u6210\u9ec3\u8272\u7522\u7269\u3002\u5728412 nm\u8655\u6e2c\u5f97\u7684\u5149\u5bc6\u5ea6\u53ef\u4ee5\u76f4\u63a5\u53cd\u6620\u6a23\u54c1\u4e2d\u7684\u7a40\u80f1\u7518\u80bd\u6fc3\u5ea6\u3002<\/td>\n<\/tr>\n
KTB1610<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit<\/p>\n

\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08GSSG\uff09\u6aa2\u6e2c<\/a><\/span><\/td>\n

\u7a40\u80f1\u7518\u80bd\u6c27\u5316\uff08GSSG\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2\uff1a\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53ef\u8207DTNB\u53cd\u61c9\u4e26\u751f\u62102-\u785d\u57fa-5-\u5df0\u57fa\u82ef\u7532\u9178\uff0c\u5728412nm\u6ce2\u9577\u8655\u5177\u6709\u6700\u5927\u7684\u5149\u5438\u6536\u7387\u3002<\/td>\n<\/tr>\n
KTB1620<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Reductases (GR) Colorimetric Assay Kit<\/p>\n

GR\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n

\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08GR\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2\uff1aGR\u53ef\u4ee5\u50ac\u5316NADPH\u9084\u539f\u70baGSSG\uff0c\u5f9e\u800c\u518d\u751fGSH\uff0cNADPH\u812b\u6c2b\u751f\u6210NADP +\uff1bNADPH\u5728340 nm\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u5728\u8a72\u6ce2\u9577\u6c92\u6709\u5438\u6536\u5cf0\u3002\u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u7387\u4f86\u8a08\u7b97GR\u6d3b\u6027\uff0c\u5f9e\u800c\u78ba\u5b9aNADPH\u812b\u6c2b\u7387\u3002<\/td>\n<\/tr>\n
KTB1630<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione S-Transferase (GST) Colorimetric Assay Kit<\/p>\n

GST\u7a40\u80f1\u7518\u80bdS-\u8f49\u79fb\u9176\u5206\u6790<\/a><\/span><\/td>\n

\u7a40\u80f1\u7518\u80bdS-\u8f49\u79fb\u9176\uff08GST\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2\uff1aGST\u50ac\u5316GSH\u8207CDNB\u7684\u7d50\u5408\u3002\u5171\u8edb\u4f34\u96a8\u8457\u5728340nm\u8655\u5438\u5149\u5ea6\u7684\u589e\u52a0\u3002\u589e\u52a0\u901f\u7387\u8207\u6a23\u54c1\u4e2d\u7684GST\u6d3b\u6027\u6210\u6b63\u6bd4\u3002<\/td>\n<\/tr>\n
KTB1640<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit<\/p>\n

GSH-Px \u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\uff08GSH-Px\uff09\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\uff1aGSH-Px\u50ac\u5316H2O2\u6c27\u5316GSH\u751f\u6210GSSG\uff1b\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08GR\uff09\u50ac\u5316NADPH\u9084\u539fGSSG\u5f9e\u800c\u518d\u751fGSH\uff0c\u800cNADPH\u6c27\u5316\u7522\u751fNADP +\u3002 NADPH\u5728340 nm\u8655\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u6c92\u6709\u3002\u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4f86\u78ba\u5b9aNADPH\u812b\u6c2b\u901f\u7387\uff0c\u4ee5\u8a08\u7b97GSH-Px\u6d3b\u6027\u3002<\/td>\n<\/tr>\n
KTB1650<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Thioredoxin Reductase (TrxR) Colorimetric Assay Kit<\/p>\n

\u00a0\u786b\u6c27\u9084\u86cb\u767d\u9084\u539f\u9176 (TrxR) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n

\u786b\u6c27\u9084\u86cb\u767d\u9084\u539f\u9176\uff08TrxR\uff09\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\uff1aTrxR\u53ef\u4ee5\u50ac\u5316NADPH\u9084\u539fDTNB\u5f9e\u800c\u751f\u6210TNB\u548cNADP +\u3002 TNB\u5728412nm\u8655\u5177\u6709\u7279\u5fb5\u5438\u6536\u5cf0\u3002 TrxR\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf412 nm\u8655TNB\u7684\u589e\u52a0\u901f\u7387\u4f86\u8a08\u7b97\u3002<\/td>\n<\/tr>\n
KTB1660<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit<\/p>\n

\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/span><\/td>\n

\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\uff1aTPX\u50ac\u5316H2O2\u6c27\u5316\u4e8c\u786b\u8607\u7cd6\u9187\uff08DTT\uff09\u3002 H2O2\u7684\u5438\u6536\u6ce2\u9577\u70ba240nm\u3002 TPX\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf240nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4e26\u6e1b\u53bb\u904e\u6c27\u5316\u6c2b\u9176\uff08CAT\uff09\u50ac\u5316\u7684H2O2\u4f86\u8a08\u7b97\u3002\u56e0\u6b64\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u540c\u6642\u6e2c\u91cf\u6a23\u54c1\u7684TPX\u548cCAT\u6d3b\u6027\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>|\u00a0Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>|\u00a0\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

GPx\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c |Glutathione Peroxidase (GPx\/GSH-Px) Act […]<\/p>\n","protected":false},"author":3,"featured_media":24734,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1482],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25377"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25377"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25377\/revisions"}],"predecessor-version":[{"id":54793,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25377\/revisions\/54793"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24734"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25377"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25377"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25377"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}