{"id":25371,"date":"2020-06-15T20:29:22","date_gmt":"2020-06-15T12:29:22","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25371"},"modified":"2023-11-28T12:05:02","modified_gmt":"2023-11-28T04:05:02","slug":"gr%e7%a9%80%e8%83%b1%e7%94%98%e8%82%bd%e9%82%84%e5%8e%9f%e9%85%b6-glutathione-reductases-gr-colorimetric-assay-kit%e8%b2%a8%e8%99%9fktb1620","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25371","title":{"rendered":"GR\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176| Glutathione Reductases (GR) Colorimetric Assay Kit\u8ca8\u865fKTB1620"},"content":{"rendered":"

GR\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u6aa2\u6e2c| Glutathione Reductases (GR) Colorimetric Assay Kit\u8ca8\u865fKTB1620<\/u><\/a><\/span><\/p>\n

\u7a40\u80f1\u7518\u80bd (Glutathione) \u7814\u7a76\u7684\u610f\u7fa9<\/span><\/span><\/p>\n

\u7a40\u80f1\u7518\u80bd\u662f\u542b\u6709\u8c37\u6c28\u9178 (glutamate)\uff0c\u534a\u80f1\u6c28\u9178(cysteine)\u548c\u7518\u6c28\u9178(glycine)\u7684\u4e09\u80bd\uff0c\u5176\u4e2d\u542b\u6709\u5df0\u57fa(sulfhydryl groups)\u3002\u5b83\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u690d\u7269\u4e2d\u3002\u5b83\u5177\u6709\u6297\u6c27\u5316\u5291(antioxidant)\u548c\u7d9c\u5408\u89e3\u6bd2(detoxification)\u4f5c\u7528\u3002\u5176\u76f8\u95dc\u7684\u8f14\u9176(coenzyme)\u662fNADPH\u3002\u4eba\u9ad4\u65b0\u9673\u4ee3\u8b1d\u7522\u751f\u7684\u81ea\u7531\u57fa(free radicals)\u904e\u591a\u6703\u7834\u58de\u751f\u7269\u819c(biofilm)\uff0c\u4fb5\u5165\u751f\u547d\u5927\u5206\u5b50\uff0c\u52a0\u901f\u4eba\u9ad4\u8870\u8001(aging)\uff0c\u4e26\u8a98\u767c\u816b\u7624(tumors)\u6216\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis) \u7684\u7522\u751f\u3002\u7a40\u80f1\u7518\u80bd\u5177\u6709\u589e\u5f37\u751f\u7269\u9ad4\u7684\u6297\u6c27\u5316\u529f\u80fd\uff0c\u4fc3\u9032\u751f\u9577\u4ee5\u53ca\u589e\u5f37\u751f\u7269\u9ad4\u5c0d\u4eba\u9ad4\u4e0d\u5229\u74b0\u5883\u7684\u5f71\u97ff\u7684\u529f\u80fd\u3002\u5b83\u7684\u4e3b\u8981\u751f\u7406\u4f5c\u7528\u662f\u6e05\u9664\u9ad4\u5167\u7684\u81ea\u7531\u57fa\u3002\u5b83\u4e5f\u662f\u4e00\u7a2e\u5f88\u597d\u7684\u89e3\u6bd2\u5291\uff0c\u53ef\u4ee5\u6e1b\u5f31\u6709\u6bd2\u7269\u8cea\u7684\u6bd2\u6027\u4e26\u5f9e\u9ad4\u5167\u6e05\u9664\u6709\u6bd2\u7269\u8cea\u3002\u7a40\u80f1\u7518\u80bd\u9084\u4fc3\u9032\u751f\u9577\u4e26\u63d0\u9ad8\u4eba\u9ad4\u514d\u75ab\u529b\u3002\u7a40\u80f1\u7518\u80bd(Glutathione)\u4e0d\u50c5\u53ef\u4ee5\u7528\u4f5c\u85e5\u7269\uff0c\u9084\u53ef\u4ee5\u7528\u4f5c\u529f\u80fd\u6027\u98df\u54c1\u7684\u57fa\u790e\u6750\u6599\uff0c\u4e26\u4e14\u5ee3\u6cdb\u7528\u65bc\u529f\u80fd\u6027\u98df\u54c1\u4e2d\uff0c\u4f8b\u5982\u6297\u8870\u8001(anti-aging)\uff0c\u589e\u5f37\u514d\u75ab\u529b(enhancing immunity)\u548c\u6297\u816b\u7624\u4f5c\u7528(anti-tumor)\u3002<\/p>\n

\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases<\/span>, GR\uff09GR\u662f\u4e00\u7a2e\u9ec3\u7d20\u86cb\u767d\u6c27\u5316\u9084\u539f\u9176\uff0c\u5ee3\u6cdb\u5b58\u5728\u65bc\u771f\u6838\u751f\u7269\u548c\u539f\u6838\u751f\u7269\u4e2d\u3002<\/span> \u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u50ac\u5316GSSG\u9084\u539f\u70baGSH\u3002<\/span> \u5b83\u662f\u7a40\u80f1\u7518\u80bd\u6c27\u5316\u9084\u539f\u5faa\u74b0\u4e2d\u7684\u95dc\u9375\u9176\u4e4b\u4e00\uff08\u901a\u5e38\u5728\u6606\u87f2\u4e2dGR\u88abTrxR\u4ee3\u66ff\uff09\u3002<\/span> \u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u50ac\u5316NADPH\u5411GSH\u9084\u539fGSSG\uff0c\u6709\u52a9\u65bc\u7dad\u6301\u9ad4\u5167GSH \/ GSSG\u7684\u6bd4\u7387\u3002<\/span> \u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u5728\u97ff\u61c9\u6c27\u5316\u61c9\u6fc0\u6e05\u9664\u6d3b\u6027\u6c27\u65b9\u9762\u8d77\u8457\u95dc\u9375\u4f5c\u7528\uff0c\u4e26\u4e14\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u4e5f\u53c3\u8207\u6297\u58de\u8840\u9178-\u7a40\u80f1\u7518\u80bd\u5faa\u74b0\u9014\u5f91\u3002\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09<\/span><\/span>\u53ef\u4ee5\u50ac\u5316NADPH\u9084\u539f\u6210GSSG\uff0c\u5f9e\u800c\u518d\u751fGSH\uff0cNADPH\u812b\u6c2b\u751f\u6210NADP +\u3002<\/span> NADPH\u5728340 nm\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u5728\u8a72\u6ce2\u9577\u6c92\u6709\u5438\u6536\u5cf0\u3002<\/span> \u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u7387\u4f86\u8a08\u7b97\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u6d3b\u6027\uff0c\u5f9e\u800c\u78ba\u5b9aNADPH\u812b\u6c2b\u7387\u3002<\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Glutathione Reductases (GR) Activity Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nGR can catalyze the reduction of NADPH to GSSG to regenerate GSH, and NADPH dehydrogenates to produce NADP +; NADPH has a characteristic absorption peak at 340 nm, while NADP + has no absorption peak at this wavelength; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GR activity.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Assay Buffer
\n\u2022 Substrate
\n\u2022 GR Cofactor<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of GR activity in serum, plasma, plant tissue\/cell lysates and other biological fluids.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
\n\u2022 The whole processes need to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
\n\u2022 In the detection of GR activity in cells, the cell number must be between 3-5 x 106, and the extraction of GR in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.
\n\u2022 Before measurement, one or two samples should be used as a pre-experiment to ensure that the change in absorbance within 180 s is linear.
\n\u2022 Generally, mammalian tissue must be diluted 2 to 5 times with Assay Buffer.
\n\u2022 The measuring process should be operated quickly and only 1 ~ 2 blank well should be needed.
\n\u2022 The reaction temperature has influence on the result. Keep the temperature at 25\u00b0C (for general species) or 37\u00b0C (for mammals).<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at -20\u00b0C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nGR is a flavin protein redox enzyme widely present in eukaryotes and prokaryotes. GR catalyzes the reduction of GSSG to GSH. It is one of the key enzymes in the glutathione redox cycle (generally GR is replaced by TrxR in insects). GR catalyzes the reduction of GSSG by NADPH to GSH, which helps maintain the GSH \/ GSSG ratio in the body. GR plays a key role in scavenging reactive oxygen species in response to oxidative stress, and GR is also involved in the ascorbate-glutathione cycle pathway.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/strong><\/td>\nCheKine\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u00a0 \u00a0 \u00a0 \u00a0CheKine<\/strong>\u7522\u54c1\u539f\u7406<\/strong>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/strong><\/td>\n<\/tr>\n
KTB1600<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31: CheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit<\/p>\n

\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6aa2\u6e2c<\/a><\/span><\/td>\n

\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2 (KTB1600)\u5229\u7528DTNB\u8207\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53cd\u61c9\u5f62\u6210\u9ec3\u8272\u7522\u7269\u3002\u5728412 nm\u8655\u6e2c\u5f97\u7684\u5149\u5bc6\u5ea6\u53ef\u4ee5\u76f4\u63a5\u53cd\u6620\u6a23\u54c1\u4e2d\u7684\u7a40\u80f1\u7518\u80bd\u6fc3\u5ea6\u3002<\/td>\n<\/tr>\n
KTB1610<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit<\/p>\n

\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08GSSG\uff09\u6aa2\u6e2c<\/a><\/span><\/td>\n

\u7a40\u80f1\u7518\u80bd\u6c27\u5316\uff08GSSG\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2\uff1a\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53ef\u8207DTNB\u53cd\u61c9\u4e26\u751f\u62102-\u785d\u57fa-5-\u5df0\u57fa\u82ef\u7532\u9178\uff0c\u5728412nm\u6ce2\u9577\u8655\u5177\u6709\u6700\u5927\u7684\u5149\u5438\u6536\u7387\u3002<\/td>\n<\/tr>\n
KTB1620<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Reductases (GR) Colorimetric Assay Kit<\/p>\n

GR\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n

\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08GR\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2\uff1aGR\u53ef\u4ee5\u50ac\u5316NADPH\u9084\u539f\u70baGSSG\uff0c\u5f9e\u800c\u518d\u751fGSH\uff0cNADPH\u812b\u6c2b\u751f\u6210NADP +\uff1bNADPH\u5728340 nm\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u5728\u8a72\u6ce2\u9577\u6c92\u6709\u5438\u6536\u5cf0\u3002\u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u7387\u4f86\u8a08\u7b97GR\u6d3b\u6027\uff0c\u5f9e\u800c\u78ba\u5b9aNADPH\u812b\u6c2b\u7387\u3002<\/td>\n<\/tr>\n
KTB1630<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione S-Transferase (GST) Colorimetric Assay Kit<\/p>\n

GST\u7a40\u80f1\u7518\u80bdS-\u8f49\u79fb\u9176\u5206\u6790<\/a><\/span><\/td>\n

\u7a40\u80f1\u7518\u80bdS-\u8f49\u79fb\u9176\uff08GST\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2\uff1aGST\u50ac\u5316GSH\u8207CDNB\u7684\u7d50\u5408\u3002\u5171\u8edb\u4f34\u96a8\u8457\u5728340nm\u8655\u5438\u5149\u5ea6\u7684\u589e\u52a0\u3002\u589e\u52a0\u901f\u7387\u8207\u6a23\u54c1\u4e2d\u7684GST\u6d3b\u6027\u6210\u6b63\u6bd4\u3002<\/td>\n<\/tr>\n
KTB1640<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit<\/p>\n

GSH-Px \u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n

\u00a0\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\uff08GSH-Px\uff09\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\uff1aGSH-Px\u50ac\u5316H2O2\u6c27\u5316GSH\u751f\u6210GSSG\uff1b\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08GR\uff09\u50ac\u5316NADPH\u9084\u539fGSSG\u5f9e\u800c\u518d\u751fGSH\uff0c\u800cNADPH\u6c27\u5316\u7522\u751fNADP +\u3002 NADPH\u5728340 nm\u8655\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u6c92\u6709\u3002\u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4f86\u78ba\u5b9aNADPH\u812b\u6c2b\u901f\u7387\uff0c\u4ee5\u8a08\u7b97GSH-Px\u6d3b\u6027\u3002<\/td>\n<\/tr>\n
KTB1650<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Thioredoxin Reductase (TrxR) Colorimetric Assay Kit<\/p>\n

\u786b\u6c27\u9084\u86cb\u767d\u9084\u539f\u9176 (TrxR) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n

\u786b\u6c27\u9084\u86cb\u767d\u9084\u539f\u9176\uff08TrxR\uff09\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\uff1aTrxR\u53ef\u4ee5\u50ac\u5316NADPH\u9084\u539fDTNB\u5f9e\u800c\u751f\u6210TNB\u548cNADP +\u3002 TNB\u5728412nm\u8655\u5177\u6709\u7279\u5fb5\u5438\u6536\u5cf0\u3002 TrxR\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf412 nm\u8655TNB\u7684\u589e\u52a0\u901f\u7387\u4f86\u8a08\u7b97\u3002<\/td>\n<\/tr>\n
KTB1660<\/a><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit<\/p>\n

\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/span><\/td>\n

\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\uff1aTPX\u50ac\u5316H2O2\u6c27\u5316\u4e8c\u786b\u8607\u7cd6\u9187\uff08DTT\uff09\u3002 H2O2\u7684\u5438\u6536\u6ce2\u9577\u70ba240nm\u3002 TPX\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf240nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4e26\u6e1b\u53bb\u904e\u6c27\u5316\u6c2b\u9176\uff08CAT\uff09\u50ac\u5316\u7684H2O2\u4f86\u8a08\u7b97\u3002\u56e0\u6b64\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u540c\u6642\u6e2c\u91cf\u6a23\u54c1\u7684TPX\u548cCAT\u6d3b\u6027\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>|\u00a0Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>|\u00a0\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/span><\/p>\n

 <\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\n\u54c1\u9805<\/td>\n\u53cd\u61c9<\/td>\n\u6e2c\u91cf\u5100\u5668<\/td>\n<\/tr>\n
\u00a0\u8ca8\u865fKTA1001<\/a><\/td>\n\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\n

\u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/p>\n

\u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/td>\n<\/tr>\n

\n

\u8ca8\u865f\u00a0KTA2010<\/a><\/p>\n

 <\/td>\n

\n

TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/p>\n

 <\/td>\n

50\u500b\u53cd\u61c9<\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/td>\n<\/tr>\n
\n

\u8ca8\u865f\u00a0KTC011001<\/a><\/p>\n

 <\/td>\n

CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/a><\/td>\n500\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx\/Em = 500\/520 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em = 484\/501 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n10MG\/100MG<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTA3030<\/a><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTA1020<\/a><\/td>\nCCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n1000\u500b\u53cd\u61c9\/10,000\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTA2030<\/a><\/td>\n\n

\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/p>\n

(BrdU\u66ff\u4ee3\u6cd5)<\/td>\n

100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/a><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (540 nm)<\/p>\n

\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/td>\n<\/tr>\n

\u8ca8\u865fKTD3001<\/a><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/td>\n\n

2000\u500b\u53cd\u61c9\/<\/p>\n

5000\u500b\u53cd\u61c9<\/td>\n

\n

OD 562 nm<\/p>\n

\u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/p>\n

50 to 1000 \u00b5g\/ml.<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1110<\/a><\/td>\n\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u03bbmax= 450 nm<\/p>\n

1- 20 U\/mL of LDH<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>PRP100153<\/a><\/td>\nIGF-I\u91cd\u7d44\u86cb\u767d<\/a><\/td>\n200UG\/1MG<\/td>\n\n

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)<\/p>\n

> 95 % as determined by SDS-PAGE<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>\u00a0KTA2020<\/a><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em=535\/615 nm.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>BMC21400<\/a><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u9810\u88dd\u96e2\u5fc3\u67f1<\/a><\/td>\n\n

1ml\u00d75<\/p>\n

3mlx5<\/td>\n

More than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTA1200<\/a><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149370 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1080<\/a><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1090<\/a><\/td>\n\u6e05\u9664\u6c2b\u6c27\u81ea\u7531\u57fa\u80fd\u529b\u6e2c\u5b9a\u8a66\u5291<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149 536nm Hydroxyl radical scavenging rate D% =\uff08ASample – AControl\uff09\u00f7\uff08ABlank – AControl\uff09\u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB2220<\/a><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

500nm Standard: 5 \u03bcmol\/mL<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1510<\/a><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u6536\u5149505 nm<\/p>\n

Standard: 5 \u03bcmol\/mL.<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1300<\/a><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u5149\u503c630nm<\/p>\n

4- 300 mg\/dL)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1100<\/a><\/td>\nLactate\u00a0<\/strong><\/a>\u00a0\u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (450 nm)<\/p>\n

0.03 mM-2 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1700<\/a><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\u5438\u6536\u5149510 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1500<\/a><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTAC\u5206\u6790<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

593 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTA0002<\/a><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n20\u500b\u53cd\u61c9\/50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KET7013<\/a><\/td>\nEliKine\u2122 Rat IL-6 ELISA Kit<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KET0003<\/a><\/td>\n\u96cc\u4e8c\u9187\uff08Estradiol , E2\uff09\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d40 pg\/ml-1500 pg\/ml<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KET6032<\/a><\/td>\nTNF<\/em>–\u03b1<\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KET6033<\/a><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KET7005<\/a><\/td>\n\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTE70003<\/a><\/td>\n\u5c0f\u9f20\u9ec3\u560c\u5464\u812b\u6c2b\u9176\/\u6c27\u5316\u9176\uff08XDH\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 22.5 ng\/L-360 ng\/L, \u6700\u4f4e\u9748\u654f\u5ea61.0 ng\/L\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f\u00a0A02051HRP<\/strong><\/a><\/td>\nHis-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0<\/a><\/td>\n50ul\/200ul<\/td>\nWB (1:5000).<\/td>\n<\/tr>\n
\u8ca8\u865fA02170MGB<\/a><\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5), \u78c1\u73e0<\/a><\/td>\n100ul\/400ul\/2ml<\/td>\nGKPIPNPLLGLDST epitope<\/td>\n<\/tr>\n
\u8ca8\u865fKTA4001<\/a><\/td>\nJC-1<\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n\n

100\u500b\u53cd\u61c9\/<\/p>\n

500\u500b\u53cd\u61c9<\/td>\n

\n

\uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/p>\n

JC-1 Stain<\/p>\n

CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1600<\/a><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

412 nm<\/p>\n

2-200 \u00b5g\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n1MG\/10MG\/100MG<\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTP3001<\/a><\/td>\n\u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2<\/a><\/td>\n\n

50\u500b\u53cd\u61c9\/<\/p>\n

200\u500b\u53cd\u61c9<\/td>\n

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) \u2022 Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>BMD00064-50UG<\/a><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/td>\n50ug\/1mg\/10mg<\/td>\n\n

\u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>\u00a0KTB1660<\/a><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n240nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1020<\/a><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD565 nm<\/p>\n

96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1040<\/a><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 540 nm<\/p>\n

2-75 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1060<\/a><\/td>\n\u904e\u6c27\u5316\u7269\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 664 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d\u70ba15.6-1000 \u00b5U\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1050<\/a><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 532 nm<\/p>\n

1-100 \u00b5M\/L<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1030<\/a><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n200-3.13 U\/ml<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTA1030<\/a><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0OD 565 nm<\/p>\n

10,00-100,0000 cells per well<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

GR\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u6aa2\u6e2c| Glutathione Reductases (GR) Colorimetric A […]<\/p>\n","protected":false},"author":3,"featured_media":24734,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1484],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25371"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25371"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25371\/revisions"}],"predecessor-version":[{"id":47405,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25371\/revisions\/47405"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24734"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25371"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25371"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25371"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}