{"id":25236,"date":"2020-06-08T17:00:01","date_gmt":"2020-06-08T09:00:01","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25236"},"modified":"2022-07-19T16:58:28","modified_gmt":"2022-07-19T08:58:28","slug":"%e7%b4%b0%e8%8f%8c%e8%a8%88%e6%95%b8%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-bacteria-counting-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk511","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25236","title":{"rendered":"\u7d30\u83cc\u8a08\u6578\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2| Bacteria Counting Colorimetric Assay Kit\u8ca8\u865fK511"},"content":{"rendered":"

\u7d30\u83cc\u8a08\u6578\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2| <\/strong>\u8ca8\u865f<\/strong>K511<\/a><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0: <\/strong>\u7d30\u83cc\u8a08\u6578\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2(Bacteria Counting Colorimetric Assay Kit, \u8ca8\u865fK511) \u662f\u4e00\u7a2e\u7c21\u55ae\u800c\u9748\u654f\u7684\u6e2c\u5b9a\uff0c\u7528\u65bc\u5b9a\u91cf\u7d30\u83cc\u6fc3\u5ea6(bacterial concentration) \u548c\u751f\u5b58\u529b(viability)\u3002\u8a72\u6e2c\u5b9a\u57fa\u65bc\u7dda\u7c92\u9ad4\u812b\u6c2b\u9176(mitochondrial dehydrogenases) \u5c07\u6c34\u6eb6\u6027\u56db\u5511\u9e7d\uff08Tetrazolium salt, WST\uff09\u88c2\u89e3\u70baformazan\u7684\u65b9\u6cd5\u3002\u7522\u751f\u7684\u984f\u8272\u8207\u6d3b\u7d30\u83cc\u7684\u6578\u91cf\u6210\u6b63\u6bd4\uff0c\u53ef\u4ee5\u4f7f\u7528\u00a0<\/strong>ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a><\/span>\uff08\u5438\u5149\u5ea6OD 460 nm\uff09\u9032\u884c\u5b9a\u91cf\u3002\u8a72\u6e2c\u5b9a\u6cd5\u9748\u654f\uff0c\u53ef\u5728\u6dfb\u52a0\u8a66\u5291\u6642\u6aa2\u6e2c\u6bcf\u5b54\u5c11\u81f310\u500b\u7d30\u83cc\u7684\u4f4e\u5bc6\u5ea6\u57f9\u990a\u7269\u7684\u751f\u9577\u3002\u8a72\u6e2c\u5b9a\u6cd5\u9084\u53ef\u7528\u65bc\u8a08\u7b97Broth\u57f9\u990a\u7269\u4e2d\u6d3b\u7d30\u83cc\u7684\u6578\u91cf\uff0c\u6216\u7814\u7a76\u5c0d\u6291\u5236\u5291\uff0c\u57f9\u990a\u57fa\u985e\u578b\uff0c\u6297\u751f\u7d20\uff0c\u751f\u9577\u548c\u7570\u6e90\u86cb\u767d\u8cea\u904e\u8868\u9054\u689d\u4ef6\u7684\u53cd\u61c9\u7684\u7d30\u83cc\u751f\u5b58\u529b\u3002<\/p>\n

\"\"<\/p>\n

WPA\u83cc\u6db2\u5bc6\u5ea6\u6e2c\u91cf\u5100\u5668<\/a><\/strong>\u00a0|\u00a0\u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>\u00a0|\u00a0Agarose<\/a>\u00a0|\u00a0\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a>\u00a0|\u00a0\u5fae\u751f\u7269\u6297\u751f\u7d20<\/a>\u00a0|\u00a0\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a>\u00a0|\u00a0CMOS\u76f8\u6a5f<\/a>\u00a0|Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>|\u00a0\u684c\u4e0a\u578b\u6ec5\u83cc\u91dc<\/a><\/strong>\u00a0<\/u><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
Detection Method<\/strong><\/td>\nAbsorbance (460 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nBacteria<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe assay is a simple method to quantify bacteria concentration and viability. Can detect bacterial growth as few as 10 bacteria per sample<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than ~90 minutes
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Electrocoupling Solution (ECS)
\n\u2022 WST Reagent (lyophilized)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/span><\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n100 assay<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em = 415\/550 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetermination of Poly P in different biological samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 A convenient tool for detecting Inorganic polyphosphate in various biological samples
\n\u2022 Sensitive
\n\u2022 Rapid<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Poly P Assay Buffer
\n\u2022 Poly P Extraction Buffer
\n\u2022 Poly P Standard (45 mer)
\n\u2022 Poly P Dye
\n\u2022 RNase
\n\u2022 DNase
\n\u2022 Proteinase K<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u83cc\u8a08\u6578\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2| \u8ca8\u865fK511 \u7522\u54c1\u63cf\u8ff0: \u7d30\u83cc\u8a08\u6578\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2(Bacteria Counting […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[1494],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25236"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25236"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25236\/revisions"}],"predecessor-version":[{"id":25237,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25236\/revisions\/25237"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25236"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25236"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}