{"id":25215,"date":"2020-06-05T18:17:51","date_gmt":"2020-06-05T10:17:51","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25215"},"modified":"2024-08-05T14:58:47","modified_gmt":"2024-08-05T06:58:47","slug":"catalase%e9%81%8e%e6%b0%a7%e5%8c%96%e6%b0%ab%e9%85%b6%e6%b4%bb%e6%80%a7%e6%aa%a2%e6%b8%ac-chekine-catalase-activity-assay-kit%e8%b2%a8%e8%99%9fktb1040","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25215","title":{"rendered":"Catalase\u904e\u6c27\u5316\u6c2b\u9176 (\u89f8\u9176) \u6d3b\u6027\u6aa2\u6e2c | CheKine\u2122 Catalase Activity Assay Kit\u8ca8\u865fKTB1040"},"content":{"rendered":"

Catalase\u904e\u6c27\u5316\u6c2b\u9176 (\u89f8\u9176) <\/em>\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Catalase Activity Assay Kit\u8ca8\u865f<\/strong>KTB1040<\/span><\/a><\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d2-75 \u00b5M (\u6700\u4f4e\u9748\u654f\u5ea62 U\/ml)\u00a0<\/span><\/span><\/p>\n

\u904e\u6c27\u5316\u6c2b\u9176\/\u89f8\u9176\uff08Catalase , CAT\uff09\u662f\u5e7e\u4e4e\u5728\u6240\u6709\u6d3b\u751f\u7269\u9ad4\u4e2d\u5e38\u898b\u7684\u6297\u6c27\u5316\u9176(antioxidant enzyme)\u3002\u5b83\u7684\u4e3b\u8981\u529f\u80fd\u662f\u50ac\u5316\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\u5206\u89e3\u70ba\u6c34(water)\u548c\u6c27\u6c23(oxygen)\uff0c\u4e26\u53bb\u9664\u9ad4\u5167\u7684\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\uff0c\u5f9e\u800c\u9632\u6b62\u7d30\u80de\u88ab\u904e\u6c27\u5316\u6c2b\u4e2d\u6bd2\u3002\u5b83\u662f\u751f\u7269\u9632\u79a6\u7cfb\u7d71(biological defense system)\u4e2d\u7684\u95dc\u9375\u9176\u4e4b\u4e00\uff0c\u70ba\u4eba\u9ad4\u63d0\u4f9b\u4e86\u6297\u6c27\u5316\u9632\u79a6\u6a5f\u5236(antioxidant defense mechanism)\u3002CheKine\u2122\u904e\u6c27\u5316\u6c2b\u9176\/\u89f8\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2(CheKine\u2122 Catalase Activity Assay Kit, KTB1040) \u53ef\u61c9\u7528\u65bc\u5404\u7a2e\u751f\u7269\u6a23\u54c1\uff08\u4f8b\u5982Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate\uff09\u4e2d\u7684\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027(catalase activity)\u6aa2\u6e2c, \u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u4fbf\u7684\u6bd4\u8272\u6e2c\u5b9a\u65b9\u6cd5\u3002 \u8a72\u6aa2\u6e2c\u8a66\u5291\u76d2\u57fa\u65bc\u904e\u6c27\u5316\u6c2b\u9176(catalase)\u8207\u7532\u9187(methanol)\u7684\u53cd\u61c9\uff0c\u5728\u6700\u4f73\u6fc3\u5ea6\u7684H2O2\u5b58\u5728\u4e0b\uff0c\u5229\u7528\u904e\u6c27\u5316\u6c2b\u9176(catalase)\u7684\u904e\u6c27\u5316\u529f\u80fd\u4f86\u6e2c\u91cf\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u3002 \u7522\u751f\u7684\u7532\u919b(formaldehyde)\u53ef\u4ee5\u5728OD 540 nm\u8655\u9032\u884c\u6bd4\u8272\u6e2c\u5b9a\u3002 \u56e0\u6b64\uff0c\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027(catalase activity)\u8207\u7372\u5f97\u7684\u4fe1\u865f\u6210\u6b63\u6bd4\u3002\u6e2c\u91cf\u7dda\u6027\u7bc4\u570d\u70ba 2-75 \u00b5M, \u6700\u4f4e\u6e2c\u91cf\u6d3b\u6027\u70ba2 U\/ml\u3002<\/span>Abbkine\u5c0d\u65bcCAT\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2(CAT Activity Assay Kit)\uff0c\u9078\u64c7\u4e86\u4e0d\u540c\u7684\u54c1\u724c\uff08Cayman Chemical\uff0cAbcam\uff0cBiovision\uff09\uff0c\u8207Abbkine CheKine\u2122\u904e\u6c27\u5316\u6c2b\u9176\uff08CAT\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08Cat\uff03\uff1aKTB1040\uff09\u9032\u884c\u4e86\u6bd4\u8f03\u3002CAT\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff0cAbbkine\u548cCayman Chemical\u54c1\u724c\u7684\u6e2c\u5b9a\u539f\u7406\u76f8\u4f3c\u3002\u7532\u9187(Methanol)\u88ab\u7528\u4f5c\u53cd\u61c9\u5e95\u7269\u3002\u800cAbcam\u548cBiovision\u5c07H2O2\u4f5c\u70ba\u53cd\u61c9\u5e95\u7269\u3002H2O2\u4e0d\u50c5\u662f\u904e\u6c27\u5316\u6c2b\u9176\uff08catalase , CAT\uff09\u7684\u55ae\u4e00\u5e95\u7269\uff0c\u6a23\u54c1\u4e2d\u7684\u5176\u4ed6\u904e\u6c27\u5316\u7269\u9176\u53ef\u80fd\u6703\u5e72\u64fe\u5be6\u9a57\u7d50\u679c\uff0c\u4e26\u4e14\u6a19\u6e96\u66f2\u7dda\u8aa4\u5dee\u53ef\u80fd\u662f\u7531\u904e\u6c27\u5316\u6c2b\u7684\u4e0d\u7a69\u5b9a\u6027\u5f15\u8d77\u7684\u3002\u5118\u7ba1\u7532\u9187(Methanol)\u662f\u904e\u6c27\u5316\u6c2b\u9176\u7684\u7368\u7279\u5e95\u7269\uff0c\u4f46\u5176\u4ed6\u904e\u6c27\u5316\u7269\u9176\u4e0d\u80fd\u4f7f\u7528\u7532\u9187(Methanol)\u4f5c\u70ba\u5e95\u7269\uff0c\u56e0\u6b64\u53ef\u4ee5\u907f\u514d\u8a0a\u865f\u5e72\u64fe\u3002<\/p>\n

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Fig. Formaldehyde standard curve. The y-axis is absorbance of standards and the x-axis is final formaldehyde concentration (uM).<\/span><\/p>\n

1.\u6a19\u6e96\u66f2\u7dda\u7684\u7e6a\u88fd\uff1a<\/p>\n

\u4ee5\u6a19\u6e96\u6eb6\u6db2\u7684\u7532\u919b\u7d42\u6fc3\u5ea6\uff08\u03bcM\uff09\u70ba\u7e31\u8ef8\uff0c\u0394AStandard\u70ba\u6a6b\u8ef8\uff0c\u7e6a\u88fd\u6a19\u6e96\u66f2\u7dda\u3002<\/p>\n

2. \u4f7f\u7528\u6a19\u6e96\u66f2\u7dda\u7dda\u6027\u8ff4\u6b78\u6240\u5f97\u7684\u65b9\u7a0b\uff0c\u4e26\u53d6\u4ee3\u6bcf\u500b\u6a23\u54c1\u7684\u6821\u6b63\u5438\u5149\u5ea6\u503c\uff0c\u8a08\u7b97\u6a23\u54c1\u7684\u7532\u919b\u6fc3\u5ea6\u3002\u7532\u919b\uff08\u03bcM\uff09=y\u00d7\uff080.17 mL\u00f70.02 mL\uff09=8.5\u00d7y<\/p>\n

3.\u4f7f\u7528\u4e0b\u5217\u516c\u5f0f\u8a08\u7b97\u6a23\u672c\u7684 CAT \u6d3b\u6027\u3002\u4e00\u500b\u55ae\u4f4d\u5b9a\u7fa9\u70ba\u572825\u2103\u4e0b\u6bcf\u5206\u9418\u7522\u751f1.0nmol\u7532\u919b\u7684\u9175\u7d20\u91cf\u3002 CAT\u6d3b\u6027=\u6a23\u54c1\u03bcM\u00f7(20\u5206\u9418)\u00d7\u6a23\u54c1\u7a00\u91cb\u5ea6=0.425\u00d7y\u00d7\u6a23\u54c1\u7a00\u91cb\u5ea6(nmol\/min\/mL)<\/p>\n\n\n\n\n\n\n\n
Brand<\/strong><\/td>\nAssay Principle<\/strong><\/td>\nSample type<\/strong><\/td>\n<\/tr>\n
Abbkine<\/td>\n\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027(catalase activity)\u6aa2\u6e2c, \u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u4fbf\u7684\u6bd4\u8272\u6e2c\u5b9a\u65b9\u6cd5\u3002 \u8a72\u6aa2\u6e2c\u8a66\u5291\u76d2\u57fa\u65bc\u904e\u6c27\u5316\u6c2b\u9176(catalase)\u8207\u7532\u9187(methanol)\u7684\u53cd\u61c9\uff0c\u5728\u6700\u4f73\u6fc3\u5ea6\u7684H2O2\u5b58\u5728\u4e0b\uff0c\u5229\u7528\u904e\u6c27\u5316\u6c2b\u9176(catalase)\u7684\u904e\u6c27\u5316\u529f\u80fd\u4f86\u6e2c\u91cf\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u3002 \u7522\u751f\u7684\u7532\u919b(formaldehyde)\u53ef\u4ee5\u5728OD 540 nm\u8655\u9032\u884c\u6bd4\u8272\u6e2c\u5b9a\u3002 \u56e0\u6b64\uff0c\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027(catalase activity)\u8207\u7372\u5f97\u7684\u4fe1\u865f\u6210\u6b63\u6bd4\u3002\u6e2c\u91cf\u7dda\u6027\u7bc4\u570d\u70ba 2-75 \u00b5M, \u6700\u4f4e\u6e2c\u91cf\u6d3b\u6027\u70ba2 U\/ml\u3002<\/td>\nTissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate<\/td>\n<\/tr>\n
Cayman Chemical<\/td>\n\u8a72\u6aa2\u6e2c\u8a66\u5291\u76d2\u57fa\u65bc\u904e\u6c27\u5316\u6c2b\u9176\u8207\u7532\u9187\u7684\u53cd\u61c9\uff0c\u5728\u6700\u4f73\u6fc3\u5ea6\u7684H2O2\u5b58\u5728\u4e0b\uff0c\u5229\u7528\u904e\u6c27\u5316\u6c2b\u9176\u7684\u904e\u6c27\u5316\u529f\u80fd\u4f86\u6e2c\u91cf\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u3002<\/span>\u00a0\u7522\u751f\u7684\u7532\u919b\u53ef\u4ee5\u5728OD 540 nm\u8655\u9032\u884c\u6bd4\u8272\u6e2c\u5b9a\u3002<\/span>\u00a0\u56e0\u6b64\uff0c\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u8207\u7372\u5f97\u7684\u4fe1\u865f\u6210\u6b63\u6bd4\u3002<\/span><\/td>\nTissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate<\/td>\n<\/tr>\n
Abcam<\/td>\n\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u904e\u6c27\u5316\u6c2b\u9176\u8207\u904e\u6c27\u5316\u6c2b\uff08H2O2\uff09\u53cd\u61c9\u751f\u6210\u6c34\u548c\u6c27\u6c23\u3002<\/span>\u00a0\u672a\u8f49\u5316\u7684H2O2\u8207\u63a2\u91dd\u53cd\u61c9\u751f\u6210\u53ef\u4ee5\u5728OD 570 nm\u8655\u9032\u884c\u6bd4\u8272\u6cd5\u6e2c\u91cf\u7684\u7522\u7269\u3002<\/span><\/td>\nSerum, Plasma, Cell Lysate, Tissue Lysate, Urine<\/td>\n<\/tr>\n
Biovision<\/td>\n\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u904e\u6c27\u5316\u6c2b\u9176\u8207\u904e\u6c27\u5316\u6c2b\uff08H2O2\uff09\u53cd\u61c9\u751f\u6210\u6c34\u548c\u6c27\u6c23\u3002<\/span>\u00a0\u672a\u8f49\u5316\u7684H2O2\u8207\u63a2\u91dd\u53cd\u61c9\u751f\u6210\u53ef\u4ee5\u5728OD 570 nm\u8655\u9032\u884c\u6bd4\u8272\u6cd5\u6e2c\u91cf\u7684\u7522\u7269\u3002<\/span><\/td>\nTissue Homogenate, Cell Lysate, erythrocyte lysate<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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(\u6587\u737b\u5f15\u7528\u51fa\u81ea<\/a>) Figure 7.<\/b>\u00a0(a<\/b>\u2013c<\/b>) Enzymatic antioxidant levels in the ethanolic extract of\u00a0C. papaya<\/span>\u00a0in the control and diabetic rats. Each bar indicates the mean \u00b1 SEM of eight rats, with\u00a0p<\/span>\u00a0< 0.05 indicating significant differences between the groups as: a\u2014control; b\u2014diabetes; c\u2014diabetic rats treated with ethanolic extract of\u00a0C. papaya<\/span>; and d\u2014diabetic rats treated with metformin.<\/p>\n

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(\u6587\u737b\u51fa\u81ea<\/a>) To assess the effect of Cd exposure on the oxidative state, the activity of catalase<\/a>\u00a0(CAT), malondialdehyde (MDA), and total antioxidant capacity (TAC) in MC3T3-E1 cells was detected using the commercial kit (Abbkine, China).<\/p>\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Catalase (CAT) Activity Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nCheKine\u2122 Micro Catalase Activity Assay Kit provides a simple and easy colorimetric assay for the study of catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. This assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Assay Buffer
\n\u2022 Sample Diluent
\n\u2022 Formaldehyde standard (4.25 M)
\n\u2022 Catalase (control)
\n\u2022 Potassium Hydroxide
\n\u2022 Hydrogen Peroxide
\n\u2022 Chromogen
\n\u2022 Potassium Periodate<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of catalase activity in serum, plasma, tissue\/cell lysates and other biological fluids.
\n\u2022 Determining catalase activity directly by utilizing the peroxidatic function of catalase, which can not be interfered by other peroxidases.
\n\u2022 A broad range linearity: 2-75 \u00b5M.
\n\u2022 Measure catalase activity down to 2 U\/ml<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 If not assayed immediately, samples can be stored at -80\u00b0C.
\n\u2022 Overheating can inactivate catalse. The enzyme should be kept cold during sample preparation and assaying.
\n\u2022 In general, catalase is very unstable at high dilution. It is recommned to store samples concentrated and assay within 30 minutes afrer dilution.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at -20\u00b0C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nCatalase (EC 1.11.1.6), is a common antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen, ubiquitously present in aerobic cells containing a cytochrome system. Hydrogen peroxide is highly deleterious to the cell and its accumulation will cause oxidation of cellular targets such as DNA, proteins, and lipids, thus leading to mutagenesis and cell death. Removal of the hydrogen peroxide (H2O2) from cells by using catalase provides protection against oxidative damage to the cell. The role of catalase in oxidative stress related diseases has been widely studied. Catalase also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. Aliphatic alcohols are specific substrates for catalase, however, other enzymes with peroxidatic activity do not utilize these substrates.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5546\u54c1\u9700\u7528\u5100\u5668\u6e2c\u91cf<\/span>\u00a0\u00a0\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100\u8acb\u6309\u6b64\u00a0<\/a><\/span>(\u6a19\u914d405\u3001450\u3001492\u3001630 nm, \u53ef\u64f4\u589e\u81f37\u500b\u6ffe\u7247)<\/p>\n

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\u6aa2\u6e2c\u8102\u8cea\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6-\uff08SOD\u3001CAT\u3001GPx\u3001MDA\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/span><\/strong><\/span><\/h1>\n

\u5224\u65b7\u6a5f\u9ad4\u6216\u7d30\u80de\u7684\u8102\u8cea\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6\uff0c\u4e00\u65b9\u9762\u9700\u8981\u6aa2\u6e2c\u6297\u6c27\u5316\u9176\uff08SOD\u3001CAT\u3001GPx\uff09\u6d3b\u529b\u4ee5\u5224\u65b7\u85e5\u7269\u7b49\u6e05\u9664\u6c27\u81ea\u7531\u57fa\u7684\u80fd\u529b\uff0c\u53e6\u4e00\u65b9\u9762\u6aa2\u6e2c\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269MDA\u7684\u6c34\u5e73\u4ee5\u9593\u63a5\u5224\u65b7\u7d30\u80de\u53d7\u81ea\u7531\u57fa\u653b\u64ca\u7684\u56b4\u91cd\u7a0b\u5ea6\u3002\u540c\u6642\u5206\u6790SOD\u3001CAT\u3001GPx\u3001MDA\u7684\u6c34\u5e73\u7d50\u679c\u5206\u6790\u53ef\u66f4\u5168\u9762\u8a55\u4f30\u6c27\u5316\u58d3\u529b\u6c34\u5e73\u7684\u8b8a\u5316\u3002\u773e\u6240\u5468\u77e5\uff0c\u6c27\u5316\u58d3\u529b(oxidative stress)\u662f\u56e0\u70ba\u6a5f\u9ad4\u6d3b\u6027\u6c27\u6c34\u5e73\u548c\u5167\u6e90\u6027\u6297\u6c27\u5316\u80fd\u529b\u4e4b\u9593\u5931\u53bb\u4e86\u5e73\u8861\uff0c\u5c0e\u81f4\u7d30\u80de\u6297\u6c27\u5316\u80fd\u529b\u4e0d\u8db3\uff0c\u7d30\u80de\u5167\u6bd2\u6027\u6d3b\u6027\u6c27\uff08ROS\uff09\u589e\u52a0\uff0c\u7522\u751f\u6c27\u81ea\u7531\u57fa\uff08 O2-\uff0c.OH, H2O2\uff09\uff0c\u4f7f\u751f\u7269\u5927\u5206\u5b50\u53d7\u5230\u653b\u64ca\uff0c\u56e0\u800c\u9020\u6210\u7684\u7d30\u80de\u548c\u7d44\u7e54\u640d\u50b7\u3002<\/p>\n

<\/p>\n

\u8102\u8cea\u6c27\u5316\u50b7\u5bb3<\/strong><\/h3>\n

\u8eab\u9ad4\u4e2d\u6c27\u5316\u9084\u539f\u72c0\u614b\u5931\u8861\u52e2\u5fc5\u6703\u9020\u6210\u56b4\u91cd\u5f8c\u679c\uff0c\u4e0d\u540c\u7d44\u7e54\u7d30\u80de\u7b49\u6703\u906d\u53d7\u5230\u4e0d\u540c\u7a0b\u5ea6\u7684\u640d\u50b7\u8207\u7834\u58de\uff0c\u4e3b\u8981\u8868\u73fe\u5728\u8102\u8cea\u3001\u80fa\u57fa\u9178\u548c\u86cb\u767d\u8cea\u7684\u6c27\u5316\u640d\u50b7\u3001\u6838\u9178\u548c\u67d3\u8272\u9ad4\u7b49\u6c27\u5316\u640d\u50b7\u65b9\u9762\u3002\u8102\u8cea\u8207ROS\uff08\u5c24\u5176\u662f\u2022 OH\uff09\u53cd\u61c9\u662f\u7d30\u80de\u6c27\u5316\u640d\u50b7\u7684\u666e\u904d\u6a5f\u5236\uff0c\u6b64\u53cd\u61c9\u4e3b\u8981\u53d6\u6c7a\u65bc\u7d30\u80de\u819c\u6d41\u52d5\u6027\u3002\u81ea\u7531\u57fa\u653b\u64ca\u4e0d\u98fd\u548c\u8102\u80aa\u9178\uff0c\u964d\u4f4e\u819c\u7684\u6d41\u52d5\u6027\uff0c\u5f71\u97ff\u7d30\u80de\u751f\u7406\u529f\u80fd\uff1b\u8102\u8cea\u904e\u6c27\u5316\u904e\u7a0b\u4e2d\u7522\u751f\u7684\u6c27\u81ea\u7531\u57fa\u53ef\u6c27\u5316\u4fee\u98fe\u7d30\u80de\u819c\u4e0a\u548c\u8cea\u5167\u7684\u9176\uff0c\u4f7f\u5176\u5931\u53bb\u6d3b\u6027\u3002\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269\uff0c\u5982\u4e19\u4e8c\u919b\uff08MDA\uff09\u80fd\u8207\u86cb\u767d\u3001\u6838\u9178\u53ca\u8166\u78f7\u8102\u7b49\u542b\u6c28\u57fa\u5316\u5408\u7269\u53cd\u61c9\uff0c\u4f7f\u4e4b\u767c\u751f\u4ea4\u806f\u3002\u8102\u8cea\u904e\u6c27\u5316\u53ef\u85c9\u7531\u7522\u751f\u7684\u81ea\u7531\u57fa\u640d\u50b7\u7d30\u80de\uff0c\u8a98\u5c0e\u7d30\u80de\u51cb\u4ea1\uff0c\u540c\u6642\u5176\u7d30\u80de\u6bd2\u6027\u53ef\u5f71\u97ff\u591a\u7a2e\u9175\u7d20\u7684\u6d3b\u6027\u53caATP\u7684\u5408\u6210\u3002<\/p>\n

<\/p>\n

\u6b63\u5e38\u60c5\u6cc1\u4e0b\uff0c\u751f\u7269\u9ad4\u4e2d\u5929\u7136\u7684\u6297\u6c27\u5316\u5291\u9632\u79a6\u7cfb\u7d71\u4e2d\u7684\u6297\u6c27\u5316\u9176\u53ef\u4ee5\u8207\u98f2\u98df\u6216\u85e5\u7269\u4e2d\u7684\u6297\u6c27\u5316\u5291\u5354\u540c\u4f5c\u7528\uff0c\u6e05\u9664\u904e\u6c27\u5316\u7269\u3002\u5176\u4e2d\u6700\u91cd\u8981\u7684\u6297\u6c27\u5316\u9175\u7d20\u5305\u62ec\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\uff0c\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\uff08GPx\uff09\u548c\u904e\u6c27\u5316\u6c2b\u9176\uff08Cat\uff09\u3002SOD\u8ca0\u8cac\u5c07\u8d85\u6c27\u9670\u96e2\u5b50\u6b67\u5316\u70ba\u904e\u6c27\u5316\u6c2b\uff0c\u800cCat\u548cGPxs\u5247\u53ef\u4ee5\u6e1b\u5c11\u904e\u6c27\u5316\u6c2b\uff0c\u963b\u6b62\u9ad8\u6bd2\u6027\u7fa5\u57fa\u81ea\u7531\u57fa\u7684\u7522\u751f\u3002\u91cd\u8981\u7684\u662f\uff0cGPxs\u9084\u53ef\u4ee5\u6e1b\u5c11\u591a\u5143\u4e0d\u98fd\u548c\u8102\u80aa\u9178\u7684\u6c2b\u904e\u6c27\u5316\u7269\uff0c\u62b5\u6d88\u8102\u8cea\u904e\u6c27\u5316\u7684\u6bd2\u6027\u4f5c\u7528\u3002<\/p>\n

<\/p>\n

\u540c\u6642\u56e0\u70ba\u8102\u8cea\u904e\u6c27\u5316\u662f\u7531\u81ea\u7531\u57fa\u555f\u52d5\u7684\uff0c\u4e0d\u98fd\u548c\u8102\u80aa\u9178\u5728\u81ea\u7531\u57fa\u548c\u4e00\u4e9b\u6c27\u5316\u5f15\u767c\u8102\u985e\u6c27\u5316\u7684\u6700\u521d\u968e\u6bb5\u5f62\u6210\u5171\u8edb\u4e8c\u70ef, \u7136\u5f8c\u5f62\u6210\u74b0\u72c0\u904e\u6c27\u5316\u7269, \u6700\u5f8c\u7684\u4e3b\u8981\u7522\u7269\u662f\u4e19\u4e8c\u919b\uff08MDA\uff09\u3002<\/p>\n

<\/p>\n

\u6240\u4ee5\uff0c\u5224\u65b7\u6a5f\u9ad4\u6216\u7d30\u80de\u7684\u8102\u8cea\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6\uff0c\u4e00\u65b9\u9762\u9700\u8981\u6aa2\u6e2c\u6297\u6c27\u5316\u9176\uff08SOD\u3001CAT\u3001GPx\uff09\u6d3b\u529b\u4ee5\u5224\u65b7\u85e5\u7269\u7b49\u6e05\u9664\u6c27\u81ea\u7531\u57fa\u7684\u80fd\u529b\uff0c\u53e6\u4e00\u65b9\u9762\u6aa2\u6e2c\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269MDA\u7684\u6c34\u5e73\u4ee5\u9593\u63a5\u5224\u65b7\u7d30\u80de\u53d7\u81ea\u7531\u57fa\u653b\u64ca\u7684\u56b4\u91cd\u7a0b\u5ea6\u3002\u540c\u6642\u5206\u6790SOD\u3001CAT\u3001GPx\u3001MDA\u7684\u6c34\u5e73\u7d50\u679c\u5206\u6790\u53ef\u66f4\u5168\u9762\u8a55\u4f30\u6c27\u5316\u58d3\u529b\u6c34\u5e73\u7684\u8b8a\u5316\u3002<\/p>\n

Abbkine \u53ef\u63d0\u4f9b\u5168\u5957\u8102\u8cea\u6c27\u5316\u640d\u50b7\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u6aa2\u6e2c\u65b9\u6cd5\u7c21\u55ae\u3001\u65b9\u4fbf\u3001\u9748\u654f\uff0c\u7522\u54c1\u8a73\u60c5\u5982\u4e0b\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n
\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u6aa2\u6e2c\u65b9\u6cd5<\/strong><\/td>\n\u9748\u654f\u5ea6<\/strong>&<\/strong>\u6aa2\u6e2c\u7bc4\u570d<\/strong><\/td>\n\u9069\u5408\u6a23\u672c<\/strong><\/td>\n\u8ca8\u865f<\/strong><\/td>\n<\/tr>\n
CheKine\u2122 \u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176(SOD) \u6d3b\u529b\u6aa2\u6e2c\u8a66\u5291\u76d2<\/td>\n\u8f03\u7a69\u5b9a\/\u9748\u654f\u7684WST-8\u986f\u8272\u6cd5<\/td>\n3.13U\/ml -200U\/ml<\/td>\n\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2<\/td>\nKTB1030<\/a><\/td>\n<\/tr>\n
\n

CheKine\u2122 Catalase (CAT)<\/p>\n

\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/td>\n

\u76f4\u63a5\u5229\u7528\u904e\u6c27\u5316\u6c2b\u9176\u7684\u904e\u6c27\u5316\u529f\u80fd\u76f4\u63a5\u6e2c\u5b9a\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027<\/td>\n\u9748\u654f\u5ea6\u4f4e\u81f32 U\/ml<\/td>\n\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d05\u8840\u7403\u88c2\u89e3\u7522\u7269\u3001\u7d44\u7e54\u52fb\u6f3f\u3001\u7d30\u80de\u88c2\u89e3\u6db2\u7b49\u751f\u7269\u6a23\u54c1<\/td>\nKTB1040<\/a><\/td>\n<\/tr>\n
CheKine\u2122\u8102\u8cea\u904e\u6c27\u5316\uff08MDA\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2<\/td>\n\u6539\u826f\u7684\u786b\u4ee3\u5df4\u6bd4\u59a5\u9178(TBA)\u6cd5<\/td>\n1-100 \u00b5M\/L<\/td>\n\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u5c3f\u6db2\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2<\/td>\nKTB1050<\/a><\/td>\n<\/tr>\n
\n

CheKine\u2122\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\uff08GSH-Px\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2<\/p>\n

 <\/td>\n

\u76f4\u63a5\u5229\u7528GSH-Px\u9176\u50ac\u5316H\u00a02<\/sub>\u00a0O\u00a02<\/sub>\u7684\u6d3b\u6027\u9032\u884c\u6aa2\u6e2c<\/td>\n\/\/<\/td>\n\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2<\/td>\nKTB1640<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u767c\u8868\u7684\u6587\u737b\uff1a<\/strong><\/span><\/p>\n

Anton Bahtiar*, Putri Sagita Utami\uff0cetc. The Antioxidant Effects of the Ethanolic Extract of Binahong Leaves Unilateral Ureteral Obstruction Rat Model\uff0cPharmacogn J. DOI : 10.5530\/pj.2021.13.26.<\/p>\n

\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5<\/strong><\/span><\/p>\n

Abbkine\u6240\u6709\u751f\u5316\u8a66\u5291\u76d2\u90fd\u53ef\u7528\u5206\u5149\u5149\u5ea6\u8a08\u6aa2\u6e2c\u7684\uff0c\u9700\u78ba\u8a8d\u4e00\u500b\u5148\u6c7a\u689d\u4ef6\uff1a\u5177\u5099\u8207\u5206\u5149\u5149\u5ea6\u8a08\u5339\u914d\u7684\u5fae\u91cf\u6bd4\u8272\u76bf\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u5206\u985e<\/span><\/td>\n\u8ca8\u865f<\/span><\/td>\n\u54c1\u9805<\/span><\/td>\n\u5167\u5bb9<\/span><\/td>\n\u66f4\u591a\u8cc7\u6599<\/span><\/td>\n<\/tr>\n
\n

\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5<\/span><\/p>\n

<\u6297\u6c27\u5316\u7dad\u751f\u7d20\u7cfb\u7d71&\u5c0f\u5206\u5b50><\/span><\/td>\n

\u8ca8\u865fKTB1070<\/span><\/td>\nCheKine\u2122<\/strong>\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/strong><\/a><\/span><\/td>\n\n

CheKine\u2122 Xanthine Oxidase Assay Kit<\/span><\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/span><\/td>\n

\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1910<\/span><\/td>\nCheKine\u2122<\/span><\/strong>\u6d3b\u6027\u6c27\uff08ROS<\/strong>\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2<\/strong><\/span><\/a><\/span>
\n<\/strong><<\/strong>\u87a2\u5149\u6aa2\u6e2c<\/strong>><\/strong><\/span><\/span><\/td>\n		\n

\u6d3b\u6027\u6c27ROS\u6aa2\u6e2c<\/span><\/p>\n

DCFH-DA (10 mM)<\/span><\/td>\n

\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/span><\/td>\n\u4e00\u6c27\u5316\u6c2e\uff08NO<\/span><\/strong>\uff09\u6aa2\u6e2c<\/strong><\/span><\/a>\u00a0<\/strong><\/span><\/span><\/td>\n\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1040<\/span><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\/\u89f8\u9176<\/em>\u6d3b\u6027\u6aa2\u6e2c<\/a><\/span><\/strong><\/td>\n\u6e2c\u91cf\u7dda\u6027\u7bc4\u570d\u70ba 2-75 \u00b5M, \u6700\u4f4e\u6e2c\u91cf\u6d3b\u6027\u70ba2 U\/ml\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1500<\/a><\/span><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\u5206\u6790<\/a><\/strong><\/span><\/td>\n\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1530<\/a><\/strong><\/span><\/td>\n\n

\u9ec3\u916e\u985e\u5316\u5408\u7269\u6aa2\u6e2c\u5957\u7d44<\/span><\/a><\/strong><\/span><\/p>\n

<<\/strong>\u690d\u7269<\/strong>><\/strong><\/span><\/td>\n

\u5075\u6e2c\u7bc4\u570d:0.156-10 mg\/g<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1050<\/span><\/td>\nCheKine\u2122\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/a><\/strong><\/span><\/td>\nMDA\u8207\u786b\u4ee3\u5df4\u6bd4\u59a5\u9178\uff08thiobarbituric acid ,TBA\uff09\u53cd\u61c9\u751f\u6210MDA-TBA\u52a0\u5408\u7269\u5728OD 532 nm\u8655\u9032\u884c\u6bd4\u8272\u5b9a\u91cf\u30021-100 \u00b5M\/L<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1091<\/span><\/td>\n\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/span><\/a><\/strong><\/span><\/td>\nHydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit\u00a0<\/a><\/strong><\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1210<\/a><\/strong><\/span><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/span><\/a><\/strong><\/span><\/td>\n\u00a0Superoxide Anion Colorimetric Assay Kit<\/a><\/strong><\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1020<\/span><\/td>\n\n

Coenzyme \u2160 NAD(H)\u00a0\u5206\u6790\u8a66\u5291\u76d2|<\/strong><\/span><\/a><\/span><\/p>\n

<\u7d44\u7e54\/\u7d30\u80de><\/span><\/td>\n

NAD\u662f\u4e00\u7a2e\u9176\u5171\u9ad4(enzymatic cofactor)\uff0c\u6d89\u53ca\u8a31\u591a\u6c27\u5316\u9084\u539f\u53cd\u61c9(redox reactions)<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1041<\/a><\/strong><\/span><\/td>\nCheKine\u2122 \u904e\u6c27\u5316\u6c2b (H2O2) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/a><\/strong><\/span><\/td>\n\u904e\u6c27\u5316\u6c2b (Hydrogen Peroxide , H2O2) \u662f\u4e00\u7a2e\u6d3b\u6027\u6c27(reactive oxygen)\u4ee3\u8b1d\u526f\u7522\u7269<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1080<\/span><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2| <\/span><\/a><\/strong><\/span><\/td>\nO2-\u8207\u56db\u5511\u9393\u9e7dWST-8\u67d3\u6599\u53cd\u61c9\u5f62\u6210\u6c34\u6eb6\u6027\u7684\u6709\u8272formazan\u7522\u7269<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\n

\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5<\/span><\/p>\n

<\u6297\u6c27\u5316\u9175\u7d20\u7cfb\u7d71><\/span><\/td>\n

\u8ca8\u865fKTB1610<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit<\/span><\/p>\n

\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08GSSG\uff09\u6aa2\u6e2c<\/span><\/strong><\/a><\/span><\/td>\n

\u7a40\u80f1\u7518\u80bd\u6c27\u5316\uff08GSSG\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2\uff1a\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53ef\u8207DTNB\u53cd\u61c9\u4e26\u751f\u62102-\u785d\u57fa-5-\u5df0\u57fa\u82ef\u7532\u9178\uff0c\u5728412nm\u6ce2\u9577\u8655\u5177\u6709\u6700\u5927\u7684\u5149\u5438\u6536\u7387\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1600<\/span><\/td>\n\u82f1\u6587\u540d\u7a31: CheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit
\n\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6aa2\u6e2c<\/a><\/strong><\/span><\/span><\/td>\n		\u5229\u7528DTNB\u8207\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53cd\u61c9\u5f62\u6210\u9ec3\u8272\u7522\u7269\u3002\u5728412 nm\u8655\u6e2c\u5f97\u7684\u5149\u5bc6\u5ea6\u53ef\u4ee5\u76f4\u63a5\u53cd\u6620\u6a23\u54c1\u4e2d\u7684\u7a40\u80f1\u7518\u80bd\u6fc3\u5ea6\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865f\u00a0KTB1620<\/span><\/td>\n\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit
\n\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08GSSG\uff09\u6aa2\u6e2c<\/strong><\/a><\/span><\/span><\/td>\n		\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53ef\u8207DTNB\u53cd\u61c9\u4e26\u751f\u62102-\u785d\u57fa-5-\u5df0\u57fa\u82ef\u7532\u9178\uff0c\u5728412nm\u6ce2\u9577\u8655\u5177\u6709\u6700\u5927\u7684\u5149\u5438\u6536\u7387\u300210-20uM<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1640<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit<\/span><\/p>\n

GSH-Px \u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c<\/span><\/strong><\/a><\/span><\/p>\n

 <\/td>\n

GSH-Px\u50ac\u5316H2O2\u6c27\u5316GSH\u751f\u6210GSSG\uff1b\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08GR\uff09\u50ac\u5316NADPH\u9084\u539fGSSG\u5f9e\u800c\u518d\u751fGSH\uff0c\u800cNADPH\u6c27\u5316\u7522\u751fNADP +\u3002 NADPH\u5728340 nm\u8655\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u6c92\u6709\u3002\u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4f86\u78ba\u5b9aNADPH\u812b\u6c2b\u901f\u7387\uff0c\u4ee5\u8a08\u7b97GSH-Px\u6d3b\u6027\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1630<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione S-Transferase (GST) Colorimetric Assay Kit<\/span><\/p>\n

GST\u7a40\u80f1\u7518\u80bdS-\u8f49\u79fb\u9176\u5206\u6790<\/span><\/strong><\/a><\/span><\/td>\n

FGST\u50ac\u5316GSH\u8207CDNB\u7684\u7d50\u5408\u3002\u5171\u8edb\u4f34\u96a8\u8457\u5728340nm\u8655\u5438\u5149\u5ea6\u7684\u589e\u52a0\u3002\u589e\u52a0\u901f\u7387\u8207\u6a23\u54c1\u4e2d\u7684GST\u6d3b\u6027\u6210\u6b63\u6bd4\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1650<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Thioredoxin Reductase (TrxR) Colorimetric Assay Kit<\/span><\/p>\n

\u00a0\u786b\u6c27\u9084\u86cb\u767d\u9084\u539f\u9176 (TrxR) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/strong><\/a><\/span><\/td>\n

TrxR\u53ef\u4ee5\u50ac\u5316NADPH\u9084\u539fDTNB\u5f9e\u800c\u751f\u6210TNB\u548cNADP +\u3002 TNB\u5728412nm\u8655\u5177\u6709\u7279\u5fb5\u5438\u6536\u5cf0\u3002 TrxR\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf412 nm\u8655TNB\u7684\u589e\u52a0\u901f\u7387\u4f86\u8a08\u7b97\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1660<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit<\/span><\/p>\n

\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/strong><\/span><\/a><\/span><\/td>\n

TPX\u50ac\u5316H2O2\u6c27\u5316\u4e8c\u786b\u8607\u7cd6\u9187\uff08DTT\uff09\u3002 H2O2\u7684\u5438\u6536\u6ce2\u9577\u70ba240nm\u3002 TPX\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf240nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4e26\u6e1b\u53bb\u904e\u6c27\u5316\u6c2b\u9176\uff08CAT\uff09\u50ac\u5316\u7684H2O2\u4f86\u8a08\u7b97\u3002\u56e0\u6b64\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u540c\u6642\u6e2c\u91cf\u6a23\u54c1\u7684TPX\u548cCAT\u6d3b\u6027\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1030<\/span><\/td>\n\n

Superoxide dismutase Activity Assay Kit,<\/a><\/strong><\/span><\/p>\n

\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n

WST-8\u6cd5\u6e2c\u5b9aSOD\u6d3b\u6027\uff0c\u6700\u5927\u6291\u5236\u7387\u53ef\u63a5\u8fd1100%\uff0c\u4e0d\u53d7\u4e00\u4e9b\u5e38\u898b\u5e72\u64fe\u56e0\u7d20\u7684\u5e72\u64fe\u3002\u5ee3\u6cdb\u7684\u7dda\u6027\u7bc4\u570d\uff1a3.13-200U\/mL\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fCEA660Ge<\/span><\/td>\n8-<\/u>\u7fa5\u57fa\u53bb\u6c27\u9ce5\u82f7<\/u>(8-OHdG)<\/u>\u6aa2\u6e2c\u8a66\u5291\u76d2<\/u>(<\/u>\u9175\u7d20\u514d\u75ab\u5206\u6790\u6cd5<\/u> <\/strong><\/span><\/td>\n\u5075\u6e2c\u7684\u9748\u654f\u5ea674.07-6000pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea627.53pg\/mL<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1011<\/strong><\/span><\/td>\nCheKine\u2122 \u8461\u8404\u7cd6-6-\u78f7\u9178\u812b\u6c2b\u9176 (G6PDH) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fKTB1011<\/span><\/strong><\/a><\/span><\/td>\n\u6a23\u54c1\u4e2d\u5b58\u5728\u7684 G6PDH \u5c07 NADP+ \u8f49\u5316\u70ba NADPH\uff0c\u5176\u5728 340 nm \u8655\u5177\u6709\u5438\u5149\u5ea6\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1140<\/a><\/strong><\/span><\/td>\n\n

PPO\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44<\/a><\/strong><\/span><\/p>\n

 <\/td>\n

Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit<\/a><\/strong><\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5<\/span>
\n <\u86cb\u767d\u8cea><\/span><\/td>\n		\u8ca8\u865fKTB120<\/a>0<\/strong><\/span><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fKTB120<\/span><\/a>0<\/span><\/strong><\/span><\/td>\n\u7fb0\u57fa\u86cb\u767d(Protein carbonyl)\u662f\u86cb\u767d\u8cea\u6c27\u5316\u4fee\u98fe(protein oxidative modification)\u904e\u7a0b\u4e2d\u65e9\u671f\u4fe1\u865f\uff0c\u662f\u8861\u91cf\u86cb\u767d\u8cea\u6c27\u5316\u640d\u50b7(protein oxidative damage)\u7684\u4e3b\u8981\u6307\u6a19<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Catalase\u904e\u6c27\u5316\u6c2b\u9176 (\u89f8\u9176) \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Catalase Activity […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1496],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25215"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25215"}],"version-history":[{"count":20,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25215\/revisions"}],"predecessor-version":[{"id":49953,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25215\/revisions\/49953"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25215"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25215"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}