{"id":25211,"date":"2020-06-05T13:09:37","date_gmt":"2020-06-05T05:09:37","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25211"},"modified":"2022-07-19T16:58:58","modified_gmt":"2022-07-19T08:58:58","slug":"%e5%a4%a7%e9%bc%a0%e8%83%b0%e5%b3%b6%e7%b4%a0-insulin-elisa%e8%a9%a6%e5%8a%91%e7%9b%92-rat-insulin-ins-elisa-kit%e8%b2%a8%e8%99%9fkte101059","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25211","title":{"rendered":"\u5927\u9f20\u80f0\u5cf6\u7d20 Insulin ELISA\u8a66\u5291\u76d2| Rat Insulin (INS) ELISA Kit\u8ca8\u865fKTE101059"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=25211\">\u5927\u9f20\u80f0\u5cf6\u7d20 (Rat Insulin , INS) ELISA\u8a66\u5291\u76d2| Rat Insulin (INS) ELISA Kit\u8ca8\u865fKTE101059<\/a><\/span><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/abbkine%E5%95%86%E5%93%81%E6%8F%92%E5%9C%96-300x207.jpg\" alt=\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%8E%9F%E7%90%86%E4%BB%8B%E7%B4%B9-300x114.png\" alt=\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\" width=\"93\" height=\"35\" \/>\u5927\u9f20\u80f0\u5cf6\u7d20 (Rat Insulin , INS) ELISA \u5957\u4ef6\u63a1\u7528\u8a66\u5291\u76d2\u63a1\u7528\u5169\u9ede\u593e\u5fc3ELISA(Sandwich ELISA)\u4f86\u5b9a\u91cf\u6a23\u54c1\u4e2d\u7684\u80f0\u5cf6\u7d20(Insulin)\u3002\u80f0\u5cf6\u7d20(Insulin)\u7279\u6709\u7684\u6297\u9ad4\u5df2\u9810\u5148\u5857\u8986\u5728\u5fae\u5b54\u677f\u4e0a\u3002\u6a19\u6e96\u548c\u6a23\u54c1\u79fb\u5165Well,\u4efb\u4f55\u80f0\u5cf6\u7d20(Insulin)\u5b58\u5728\u90fd\u53d7\u56fa\u5b9a\u6297\u9ad4\u7684\u7d04\u675f\u3002\u53bb\u9664\u4efb\u4f55\u672a\u7d50\u5408\u7684\u7269\u8cea\u540e,HRP-\u7d50\u5408\u5c0f\u9f20\u80f0\u5cf6\u7d20\u6aa2\u6e2c\u6297\u9ad4\u88ab\u6dfb\u52a0\u5230Well\u3002\u6d17\u6ecc\u53bb\u9664\u4efb\u4f55\u672a\u7d50\u5408\u4e4b\u7684 HRP \u8a66\u5291\u5f8c,\u5c07 Chromogen \u6eb6\u6db2\u6dfb\u52a0\u5230Well,\u984f\u8272\u8207\u521d\u59cb\u6b65\u9a5f\u4e2d\u80f0\u5cf6\u7d20\u7d50\u5408\u91cf\u6210\u6bd4\u4f8b\u3002\u505c\u6b62\u986f\u8272\u4e26\u6e2c\u91cf\u984f\u8272\u7684\u5f37\u5ea6\u3002\u5075\u6e2c\u7bc4\u570d: 1.25 mU\/L-20 mU\/L, \u6700\u4f4e\u9748\u654f\u5ea60.1 mU\/L\u3002\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: Other biological fluids, Plasma, Serum, Cell culture supernatants.<br \/>\nRat Insulin (INS) ELISA Kit employs a two-site sandwich ELISA to quantitate Insulin in samples. An antibody specific for Insulin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Insulin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Rat Insulin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Insulin bound in the initial step. The color development is stopped and the intensity of the color is measured.<\/p>\n<ul>\n<li><strong>Detection method<\/strong><\/li>\n<\/ul>\n<p>Colorimetric<\/p>\n<ul>\n<li><strong>Sample type<\/strong><\/li>\n<\/ul>\n<p>Cell culture supernatants, Other biological fluids, Plasma, Serum<\/p>\n<ul>\n<li><strong>Assay type<\/strong><\/li>\n<\/ul>\n<p>Sandwich ELISA<\/p>\n<ul>\n<li><strong>Calibration range<\/strong><\/li>\n<\/ul>\n<p>1.25 mU\/L-20 mU\/L<\/p>\n<ul>\n<li><strong>Limit of detection<\/strong><\/li>\n<\/ul>\n<p>0.1 mU\/L<\/p>\n<p>\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805 (\u7d30\u9805\u4ee5\u539f\u5ee0\u64cd\u4f5c\u624b\u518a\u70ba\u4e3b)<\/p>\n<ul>\n<li>\u8acb\u52ff\u6df7\u7528\u4e0d\u540c\u8a66\u5291\u76d2\u4e2d\u7684\u7d44\u5206\uff0c\u6216\u5728\u8a66\u5291\u76d2\u5230\u671f\u65e5\u671f\u4e4b\u5f8c\u4f7f\u7528\u8a66\u5291\u3002<\/li>\n<li>\u6253\u958b\u4e4b\u524d\uff0c\u8b93\u6240\u6709\u8a66\u5291\u81f3\u5c11\u52a0\u5ba4\u6eab30\u5206\u9418\u3002<\/li>\n<li>\u7528\u8a66\u5291\u9810\u6e05\u6d17\u79fb\u6db2\u5668\u5438\u982d\uff0c\u70ba\u6bcf\u500b\u6a23\u54c1\uff0c\u6a19\u6e96\u6db2\u548c\u8a66\u5291\u4f7f\u7528\u65b0\u9bae\u7684\u79fb\u6db2\u5668\u5438\u982d\uff0c\u4ee5\u514d\u6c61\u67d3\u3002<\/li>\n<li>\u672a\u4f7f\u7528\u7684\u5b54\u5fc5\u9808\u5728\u63d0\u4f9b\u7684\u5bc6\u5c01\u888b\u4e2d\u65bc4\u00b0C\u4e7e\u71e5\u3002<\/li>\n<li>\u5fb9\u5e95\u6df7\u5408\u5c0d\u65bc\u7d50\u679c\u975e\u5e38\u91cd\u8981\u3002\u5efa\u8b70\u6bcf10\u5206\u9418\u4f7f\u7528\u4f4e\u983b\u632f\u76ea\u5668\u6216\u7a0d\u7a0d\u6416\u52d5\u624b\u3002<\/li>\n<li>\u5efa\u8b70\u6240\u6709\u6a23\u54c1\u548c\u6a19\u6e96\u54c1\u4e00\u5f0f\u5169\u4efd\u6216\u4e00\u5f0f\u4e09\u4efd\u9032\u884c\u6e2c\u5b9a\u3002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5927\u9f20\u80f0\u5cf6\u7d20 (Rat Insulin , INS) ELISA\u8a66\u5291\u76d2| Rat Insulin (INS) E [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":24540,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1498],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25211"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25211"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25211\/revisions"}],"predecessor-version":[{"id":28614,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25211\/revisions\/28614"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24540"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25211"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25211"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25211"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}