{"id":25184,"date":"2020-06-03T20:42:42","date_gmt":"2020-06-03T12:42:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25184"},"modified":"2026-04-10T14:23:17","modified_gmt":"2026-04-10T06:23:17","slug":"%e8%b6%85%e6%b0%a7%e5%8c%96%e7%89%a9%e6%ad%a7%e5%8c%96%e9%85%b6%ef%bc%88sod%ef%bc%89%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-superoxide-dismutase-activity-assay-kit%e8%b2%a8","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25184","title":{"rendered":"\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Superoxide dismutase Activity Assay Kit\u8ca8\u865fKTB1030"},"content":{"rendered":"

\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Superoxide dismutase Activity Assay Kit, \u8ca8\u865fKTB1030<\/a><\/span><\/strong><\/span><\/p>\n

\u6700\u5927\u6291\u5236\u7387\u53ef\u63a5\u8fd1100%\uff0c\u4e0d\u53d7\u4e00\u4e9b\u5e38\u898b\u5e72\u64fe\u56e0\u7d20\u7684\u5e72\u64fe\u3002\u5ee3\u6cdb\u7684\u7dda\u6027\u7bc4\u570d\uff1a3.13-200U\/mL\u3002Determination of SOD enzyme activity in serum, plasma, plant or animal tissue\u3001cell lysates and other biological fluids.<\/span><\/p>\n

\u6e2c\u91cf\u5100\u5668:\u00a0 450nm \u5438\u5149\u5ea6\u7684\u9176\u6a19\u5100 (ELISA Reader) \u6216\u53ef\u898b\u5206\u5149\u5149\u5ea6\u8a08 (\u9700\u914d\u5408\u5fae\u91cf\u6bd4\u8272\u7ba1)<\/p>\n

\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08Superoxide dismutases , SOD\uff0cEC1.15.1.1\uff09\u662f\u50ac\u5316\u8d85\u6c27\u5316\u7269\u9670\u96e2\u5b50(superoxide anion)\u5206\u89e3\u70baO2\u548cH2O2\u7684\u9176\u3002 \u5b83\u5011\u662f\u62b5\u6297\u66b4\u9732\u65bcO2\u7684\u6240\u6709\u7d30\u80de\u4e2d\u8d85\u6c27\u81ea\u7531\u57fa(superoxide radical)\u6bd2\u6027\u7684\u91cd\u8981\u6297\u6c27\u5316\u5291(antioxidant defense against)\u3002 SOD\u7570\u5e38\u6d3b\u52d5\u8207\u808c\u840e\u7e2e\u6027\u5074\u7d22\u786c\u5316(amyotrophic lateral sclerosis)\uff0c\u570d\u7522\u671f\u81f4\u6b7b\u7387(perinatal lethality)\uff0c\u795e\u7d93\u75be\u75c5(neural disorders)\u548c\u764c\u75c7(cancer)\u7b49\u75be\u75c5\u6709\u95dc\u3002 \u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176(superoxide dismutase)\u6709\u4e09\u500b\u4e3b\u8981\u5bb6\u65cf\uff1aCu \/ Zn\uff0cFe \/ Mn\u548cNi\u578b\u3002 \u4eba\u985e\u4e2d\u5b58\u5728\u4e09\u7a2e\u5f62\u5f0f\u7684\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176(superoxide dismutase)\u3002 SOD1\u4f4d\u65bc\u7d30\u80de\u8cea(cytoplasm)\u4e2d\uff0cSOD2\u4f4d\u65bc\u7dda\u7c92\u9ad4(mitochondria)\u4e2d\uff0cSOD3\u4f4d\u65bc\u7d30\u80de\u5916(extracellular)\u3002\u6e2c\u5b9a\u8840\u6e05 (serum)<\/span>\u3001\u8840\u6f3f\u3001\u690d\u7269(plant<\/span>)<\/span>\u3001\u52d5\u7269\u7d44\u7e54(animal tissue)<\/span>\/\u7d30\u80de\u88c2\u89e3\u6db2\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2\u4e2d\u7684 SOD \u9176\u6d3b\u6027\u3002\u00a0WST-8\u6cd5\u6e2c\u5b9aSOD\u6d3b\u6027\uff0c\u6700\u5927\u6291\u5236\u7387\u53ef\u63a5\u8fd1100%\uff0c\u4e0d\u53d7\u4e00\u4e9b\u5e38\u898b\u5e72\u64fe\u56e0\u7d20\u7684\u5e72\u64fe\u3002\u5ee3\u6cdb\u7684\u7dda\u6027\u7bc4\u570d\uff1a3.13-200U\/mL\u3002<\/span><\/p>\n

<\/p>\n

\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176 (SOD) \u662f\u4e00\u7a2e\u6297\u6c27\u5316\u9176\uff0c\u53c3\u8207\u91dd\u5c0d\u6d3b\u6027\u6c27 (ROS) \u7684\u9632\u79a6\u7cfb\u7d71\u3002SOD\u50ac\u5316\u8d85\u6c27\u81ea\u7531\u57fa\u9670\u96e2\u5b50(O 2\u00a0-)\u6b67\u5316\u53cd\u61c9\u751f\u6210\u904e\u6c27\u5316\u6c2b\uff0c\u904e\u6c27\u5316\u6c2b\u518d\u88ab\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u548c\u904e\u6c27\u5316\u6c2b\u9176\u50ac\u5316\u751f\u6210\u7121\u5bb3\u7684O\u00a02<\/sub>\u548cH\u00a02<\/sub>\u00a0O\u3002\u5df2\u7d93\u78ba\u5b9a\u4e86\u5e7e\u7a2e\u985e\u578b\u7684 SOD\u3002\u9019\u4e9b\u5305\u62ec\u7d30\u80de\u5167\u9285\u3001\u92c5 SOD\uff08Cu\u3001Zn SOD\/SOD1\uff09\u3001\u7dda\u7c92\u9ad4\u9333 SOD\uff08Mn SOD\/SOD2\uff09\u548c\u7d30\u80de\u5916 Cu\u3001Zn SOD\uff08EC SOD\/SOD3\uff09\u3002CheKine\u2122\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2(CheKine\u2122 Superoxide dismutase (SOD) Activity Assay Kit, \u8ca8\u865fKTB1030)\u70ba\u5b9a\u91cf\u6e2c\u5b9a\u8840\u6e05(serum)\uff0c\u8840\u6f3f(plasma)\uff0c\u7d44\u7e54(tissue)\/\u7d30\u80de\u88c2\u89e3\u6db2(cell lysates)\u548c\u5176\u4ed6\u751f\u7269\u6db2\u9ad4(biological fluids)\u4e2d\u7684SOD\u9176\u6d3b\u6027(SOD enzyme activity)\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u4fbf\u7684\u6bd4\u8272\u6e2c\u5b9a\u6cd5(colorimetric assay)\u3002 \u5728\u6e2c\u5b9a\u4e2d\uff0c\u9ec3\u560c\u5464\u6c27\u5316\u9176\uff08xanthine oxidase, XO\uff09\u50ac\u5316\u53cd\u61c9\u63d0\u4f9b\u4e86\u8d85\u6c27\u9670\u96e2\u5b50\uff08superoxide anion , O2-\uff09\u3002 O2-\u8207\u56db\u5511\u9393\u9e7dWST-8\u67d3\u6599( tetrazolium salt WST-8 dye)\u53cd\u61c9\u751f\u6210\u6c34\u6eb6\u6027 formazan\u7522\u7269\u3002 SOD\u6e05\u9664\u4e86O2\uff0d\uff0c\u56e0\u6b64\u7528\u65bc\u986f\u8272\u53cd\u61c9\u7684O2\uff0d\u8f03\u5c11\u3002 \u901a\u904e\u6bd4\u8272\u6cd5\u5728OD 450nm\u4e0b\u6e2c\u91cfSOD\u7684\u9019\u7a2e\u6291\u5236\u6d3b\u6027\u3002\u7531\u65bcSOD\u8a66\u5291\u76d2\uff08KTB1030\uff09\u5831\u544a\u70ba\u6291\u5236\uff05\uff0c\u56e0\u6b64\u53ef\u4ee5\u4e0d\u4f7f\u7528\u6a19\u6e96\u8a66\u5291\u3002<\/span> \u6a23\u54c1\u4e2d\u7684SOD\u8d8a\u591a\uff0c\u7522\u751f\u7684 less\u00a0 WST-8 formazan\u7522\u7269\uff08\u9019\u53d6\u6c7a\u65bc\u9ec3\u560c\u5464\u6c27\u5316\u9176\uff08XO\uff09\u6d3b\u6027\uff09\u3002<\/span><\/p>\n

\u512a\u52e2\u53ca\u7522\u54c1\u7279\u6027 \uff1a\u6bd4\u8f03NBT(\u6c2e\u85cd\u56db\u5511)\u6cd5\u3001\u7d30\u80de\u8272\u7d20C\u6cd5\uff0c WST-1\u6cd5<\/span><\/strong><\/span><\/p>\n

    \n
  1. \u7522\u751f\u7a69\u5b9a\u7684\u53cd\u61c9\u7522\u7269\u3001\u9748\u654f\u5ea6\u8f03\u9ad8\uff1b<\/li>\n
  2. \u900f\u904e\u55ae\u4e00\u6642\u9593\u9ede\u7684\u5438\u5149\u5ea6\u6aa2\u6e2c\u4f86\u6e2c\u5b9aSOD\u6d3b\u529b\uff0c\u9069\u5408\u9ad8\u901a\u91cf\u7be9\u9078\u7814\u7a76\uff1b<\/li>\n
  3. \u6700\u5927\u6291\u5236\u767e\u5206\u7387\u53ef\u4ee5\u63a5\u8fd1100%\uff0c\u4e0d\u53d7\u4e00\u4e9b\u5e38\u898b\u7684\u5e72\u64fe\u56e0\u7d20\u5e79\u64fe\uff0c\u8a31\u591a\u7d30\u80de\u548c\u7d44\u7e54\u6a23\u672c\u4e2d\u542b\u6709\u5167\u6e90\u6027\u7684\u904e\u6c27\u5316\u6c2b\uff0c\u6703\u5e72\u64feSOD\u7684\u6aa2\u6e2c\uff1b<\/li>\n
  4. \u8a08\u7b97\u65b9\u6cd5\u4fbf\u6377\uff0c\u7dda\u6027\u7bc4\u570d3.13 -200 U\/ml\uff0c\u900f\u904e\u6a19\u66f2\u53ef\u4ee5\u76f4\u63a5\u8a08\u7b97\u5f97\u5230\u9175\u7d20\u6d3bU\/ml\uff1b<\/li>\n
  5. \u61c9\u7528\u7bc4\u570d\u5ee3\u6cdb\uff1a\u6e2c\u5b9a\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u6db2\u53ca\u5176\u4ed6\u751f\u7269\u6db2\u4e2dSOD\u9176\u7684\u6d3b\u6027\uff0c\u76f4\u63a5\u88c2\u89e3\u7d30\u80de\uff0c\u7121\u9700\u52fb\u6f3f\uff0c\u64cd\u4f5c\u66f4\u4fbf\u6377\u3002<\/li>\n<\/ol>\n

    \u64cd\u4f5c\u6d41\u7a0b (Assay procedure)\u00a0<\/strong><\/span><\/p>\n

    1. \u4f7f\u7528\u524d\u914d\u88fd\u5de5\u4f5c\u8a66\u5291(Working Reagent)\u3002<\/p>\n

    \u5c0d\u65bc\u6bcf\u500b\u5b54\uff0c\u6df7\u5408 74 \u03bcL \u6aa2\u6e2c\u7de9\u885d\u6db2(Assay Buffer)\u30015 \u03bcL\u9ec3\u560c\u5464(Xanthine)\u30015 \u03bcL WST-8 \u548c 1\u03bcL \u589e\u5f37\u5291(Enhancer.)\u3002\u6df7\u5408\u597d\uff0c\u653e\u5728\u4e00\u65c1\u3002\u5c07\u6eb6\u6db2\u5728 37 \u00b0C\uff08\u5c0d\u65bc\u54fa\u4e73\u52d5\u7269\uff09\u6216 25 \u00b0C\uff08\u5c0d\u65bc\u5176\u4ed6\u52d5\u7269\uff09\u6e2c\u91cf\u524d\u653e\u7f6e5\u5206\u9418\u4ee5\u4e0a\uff08\u9632\u6b62\u84b8\u767c\uff09\u5efa\u8b70\u5305\u88f9\u76e4\u5b50\u3002<\/p>\n

    2. \u4f9d\u7167\u5efa\u8b70\u6e96\u5099\u6a23\u54c1\u3002\u5c07\u4ee5\u4e0b\u8a66\u5291\u52a0\u5165\u5fae\u5b54\u677f\u4e2d\u6df7\u52fb\uff0c37\u2103\u5b75\u80b230 min\uff0c450 nm\u8b80\u53d6\u5149\u5bc6\u5ea6\u3002<\/p>\n

    <\/p>\n

    \u0394A \u6a23\u54c1 = A \u6a23\u54c1-A \u6a23\u54c1\u5c0d\u7167\uff0c\u0394ABlank = ABlank – A \u7a7a\u767d\u5c0d\u7167\u3002<\/p>\n

    \u7a7a\u767d\u5b54\u548c\u7a7a\u767d\u5c0d\u7167\u5b54\u61c9\u5206\u5225\u8a2d\u7f6e\u4e00\u500b\u5b54\u3002\u6bcf\u500b\u4e0d\u540c\u984f\u8272\u7684\u6a23\u54c1\u61c9\u8a2d\u5b9a\u76f8\u61c9\u7684\u5c0d\u7167\u5b54\uff0c\u4ee5\u6d88\u9664\u6a23\u54c1\u672c\u8eab\u7684\u80cc\u666fOD\u503c\u3002<\/p>\n

    \u76e1\u91cf\u4fdd\u6301\u6a23\u672c\u7684\u6291\u5236\u767e\u5206\u6bd4\u572810-90%\u7bc4\u570d\u5167\u3002\u5982\u679c\u8a08\u7b97\u51fa\u7684\u6291\u5236\u767e\u5206\u6bd4\u5c0f\u65bc10%\u6216\u5927\u65bc90%\uff0c\u901a\u5e38\u9700\u8981\u8abf\u6574\u6a23\u54c1\u91cf\u3002\u82e5\u6e2c\u5b9a\u7684\u6291\u5236\u7387\u904e\u9ad8\uff0c\u9700\u7528\u6a23\u54c1\u7a00\u91cb\u6db2\u5c0d\u6a23\u54c1\u9032\u884c\u9069\u7576\u7a00\u91cb\uff1b\u5982\u679c\u6e2c\u5b9a\u7684\u6291\u5236\u7387\u8f03\u4f4e\uff0c\u5247\u9700\u8981\u63d0\u9ad8\u6a23\u54c1\u6fc3\u5ea6\u3002<\/p>\n

    <\/p>\n

     <\/p>\n

    <\/p>\n

    (\u5f15\u7528\u6587\u737b<\/a>) Colorimetric determination of MDA and SOD concentrations in rat placenta (n<\/span>\u00a0= 5). Each data point represents the mean \u00b1 S.E.M.\u00a0#<\/sup>\u00a0p<\/span>\u00a0< 0.05 and\u00a0##<\/sup>\u00a0p<\/span>\u00a0< 0.01 versus CK.<\/p>\n

    <\/p>\n

    (\u5f15\u7528\u6587\u737b<\/a>) \u00a0(a<\/b>\u2013d<\/b>) Role of\u00a0C. papaya<\/span>\u00a0on enzymatic antioxidants levels.\u00a0p<\/span>\u00a0< 0.05 was considered statistically significant among the groups: *-control; #-diabetes;\u00a0$-C. papaya<\/span>-treated T2DM.<\/p>\n

    <\/p>\n

    (\u5f15\u7528\u6587\u737b<\/a>) Effect of treatment with CCNE (100 and 400\u00a0mg\/kg) on AChE (A<\/strong>), MDA level (B<\/strong>),\u00a0SOD<\/a>\u00a0(C<\/strong>),\u00a0CAT<\/a>\u00a0(D<\/strong>) and\u00a0GPx<\/a>\u00a0(E<\/strong>). Results were expressed as mean\u00a0\u00b1\u00a0SD (n\u00a0=\u00a06) and comparison between groups was analysed using one way ANOVA with Newman-Keuls multiple comparison test. **p\u00a0<\u00a00.001 vs NCA group; ##p\u00a0<\u00a00.001 vs CCA group; $$p\u00a0<\u00a00.05 vs CCA group.<\/p>\n

    <\/p>\n

    (\u5f15\u7528\u6587\u737b<\/a>) Outcome of C. papaya on the enzymatic antioxidants levels in the control and treated rats. Each bar depicts the mean \u00b1 SEM of eight rats, with p<\/span>\u00a0< 0.05 designating statistically significant differences between the groups as follows: p\u2014control; q\u2014diabetes; r\u2014T2DM rats + C. papaya; s\u2014T2DM rats + Metformin.<\/p>\n

    <\/p>\n

    (\u5f15\u7528\u6587\u737b<\/a>) Effects of AuNPs on: (1) malondialdehyde (MDA) levels; (2) superoxide dismutase (SOD) activity level in skeletal tissue. (ND: Non-Diabetic group, D: Diabetic group, D + AuNPs: Diabetic treated with AuNPs, \u2217P<\/em>\u00a0< 0.05, \u2217\u2217P<\/em>\u00a0< 0.01, #P<\/em>\u00a0< 0.1).<\/span><\/p>\n

    <\/p>\n

    (\u5f15\u7528\u6587\u737b<\/a>) miR-424-5p upregulation alleviates ISO-induced inflammatory response and oxidative stress. After treating the hESC-derived neurons with or without 1.00% of ISO for 24\u2009h, the follow-up experiments were conducted. (a) ELISA was employed for the evaluation of IL-6 and TNF-\u03b1\u00a0levels after transfection of NC mimics or miR-424-5p mimics. (b) The levels of SOD, GSH, and MDA in hESC-derived neurons after transfection were evaluated through the relative kits.\u00a0,\u00a0,\u00a0and\u00a0.<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
    Product name<\/strong><\/td>\nCheKine\u2122 Micro Superoxide Dismutases (SOD) Activity Assay Kit<\/a><\/td>\n<\/tr>\n
    Applications notes<\/strong><\/td>\nAbbkine CheKine\u2122 Micro Superoxide dismutase (SOD) Activity Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of SOD enzyme activity in serum, plasma, tissue\/cell lysates and other biological fluids. In the assay, superoxide anion (O2\uff0d) is provided by xanthine oxidase (XO) catalyzed reaction. O2\uff0d reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product. SOD scavenges the O2\uff0d thus less O2\uff0d is available for the chromogenic reaction. This inhibition activity of<\/td>\n<\/tr>\n
    Kit components<\/strong><\/td>\n\u2022 Assay Buffer
    \n\u2022Sample Diluent
    \n\u2022Lysis Buffer (5\u00d7)
    \n\u2022WST-8<\/td>\n<\/tr>\n
    Features & Benefits<\/strong><\/td>\n\u2022 Determination of SOD enzyme activity in serum, plasma, plant or animal tissue\u3001cell lysates and other biological fluids.
    \n\u2022 Determining SOD activity by WST-8 method, the maximum inhibition percentage can be close to 100 %, and it can not be interfered by some common interfering factors.<\/td>\n<\/tr>\n
    Usage notes<\/strong><\/td>\n\u2022 Briefly centrifuge small vials at low speed prior to opening.
    \n\u2022 Prior to assay, bring all reagents to room temperature (25\u00b0C). The Xanthine reagent may appear to be turbid. Briefly vortext this tube before pipetting.
    \n\u2022 If not assayed immediately, samples can be stored at -80\u00b0C.<\/td>\n<\/tr>\n
    Storage instructions<\/strong><\/td>\nStorage at -20\u00b0C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.<\/td>\n<\/tr>\n
    Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

     <\/p>\n

    \"\"<\/a><\/p>\n

    Fig. Plot the Standard Curve inhibition rate vs SOD activity.<\/p>\n

    \u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/span><\/p>\n

    \u6aa2\u6e2c\u8102\u8cea\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6-\uff08SOD\u3001CAT\u3001GPx\u3001MDA\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/span><\/strong><\/span><\/h1>\n

    \u5224\u65b7\u6a5f\u9ad4\u6216\u7d30\u80de\u7684\u8102\u8cea\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6\uff0c\u4e00\u65b9\u9762\u9700\u8981\u6aa2\u6e2c\u6297\u6c27\u5316\u9176\uff08SOD\u3001CAT\u3001GPx\uff09\u6d3b\u529b\u4ee5\u5224\u65b7\u85e5\u7269\u7b49\u6e05\u9664\u6c27\u81ea\u7531\u57fa\u7684\u80fd\u529b\uff0c\u53e6\u4e00\u65b9\u9762\u6aa2\u6e2c\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269MDA\u7684\u6c34\u5e73\u4ee5\u9593\u63a5\u5224\u65b7\u7d30\u80de\u53d7\u81ea\u7531\u57fa\u653b\u64ca\u7684\u56b4\u91cd\u7a0b\u5ea6\u3002\u540c\u6642\u5206\u6790SOD\u3001CAT\u3001GPx\u3001MDA\u7684\u6c34\u5e73\u7d50\u679c\u5206\u6790\u53ef\u66f4\u5168\u9762\u8a55\u4f30\u6c27\u5316\u58d3\u529b\u6c34\u5e73\u7684\u8b8a\u5316\u3002\u773e\u6240\u5468\u77e5\uff0c\u6c27\u5316\u58d3\u529b(oxidative stress)\u662f\u56e0\u70ba\u6a5f\u9ad4\u6d3b\u6027\u6c27\u6c34\u5e73\u548c\u5167\u6e90\u6027\u6297\u6c27\u5316\u80fd\u529b\u4e4b\u9593\u5931\u53bb\u4e86\u5e73\u8861\uff0c\u5c0e\u81f4\u7d30\u80de\u6297\u6c27\u5316\u80fd\u529b\u4e0d\u8db3\uff0c\u7d30\u80de\u5167\u6bd2\u6027\u6d3b\u6027\u6c27\uff08ROS\uff09\u589e\u52a0\uff0c\u7522\u751f\u6c27\u81ea\u7531\u57fa\uff08 O2-\uff0c.OH, H2O2\uff09\uff0c\u4f7f\u751f\u7269\u5927\u5206\u5b50\u53d7\u5230\u653b\u64ca\uff0c\u56e0\u800c\u9020\u6210\u7684\u7d30\u80de\u548c\u7d44\u7e54\u640d\u50b7\u3002<\/p>\n

    <\/p>\n

    \u8102\u8cea\u6c27\u5316\u50b7\u5bb3<\/strong><\/h3>\n

    \u8eab\u9ad4\u4e2d\u6c27\u5316\u9084\u539f\u72c0\u614b\u5931\u8861\u52e2\u5fc5\u6703\u9020\u6210\u56b4\u91cd\u5f8c\u679c\uff0c\u4e0d\u540c\u7d44\u7e54\u7d30\u80de\u7b49\u6703\u906d\u53d7\u5230\u4e0d\u540c\u7a0b\u5ea6\u7684\u640d\u50b7\u8207\u7834\u58de\uff0c\u4e3b\u8981\u8868\u73fe\u5728\u8102\u8cea\u3001\u80fa\u57fa\u9178\u548c\u86cb\u767d\u8cea\u7684\u6c27\u5316\u640d\u50b7\u3001\u6838\u9178\u548c\u67d3\u8272\u9ad4\u7b49\u6c27\u5316\u640d\u50b7\u65b9\u9762\u3002\u8102\u8cea\u8207ROS\uff08\u5c24\u5176\u662f\u2022 OH\uff09\u53cd\u61c9\u662f\u7d30\u80de\u6c27\u5316\u640d\u50b7\u7684\u666e\u904d\u6a5f\u5236\uff0c\u6b64\u53cd\u61c9\u4e3b\u8981\u53d6\u6c7a\u65bc\u7d30\u80de\u819c\u6d41\u52d5\u6027\u3002\u81ea\u7531\u57fa\u653b\u64ca\u4e0d\u98fd\u548c\u8102\u80aa\u9178\uff0c\u964d\u4f4e\u819c\u7684\u6d41\u52d5\u6027\uff0c\u5f71\u97ff\u7d30\u80de\u751f\u7406\u529f\u80fd\uff1b\u8102\u8cea\u904e\u6c27\u5316\u904e\u7a0b\u4e2d\u7522\u751f\u7684\u6c27\u81ea\u7531\u57fa\u53ef\u6c27\u5316\u4fee\u98fe\u7d30\u80de\u819c\u4e0a\u548c\u8cea\u5167\u7684\u9176\uff0c\u4f7f\u5176\u5931\u53bb\u6d3b\u6027\u3002\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269\uff0c\u5982\u4e19\u4e8c\u919b\uff08MDA\uff09\u80fd\u8207\u86cb\u767d\u3001\u6838\u9178\u53ca\u8166\u78f7\u8102\u7b49\u542b\u6c28\u57fa\u5316\u5408\u7269\u53cd\u61c9\uff0c\u4f7f\u4e4b\u767c\u751f\u4ea4\u806f\u3002\u8102\u8cea\u904e\u6c27\u5316\u53ef\u85c9\u7531\u7522\u751f\u7684\u81ea\u7531\u57fa\u640d\u50b7\u7d30\u80de\uff0c\u8a98\u5c0e\u7d30\u80de\u51cb\u4ea1\uff0c\u540c\u6642\u5176\u7d30\u80de\u6bd2\u6027\u53ef\u5f71\u97ff\u591a\u7a2e\u9175\u7d20\u7684\u6d3b\u6027\u53caATP\u7684\u5408\u6210\u3002<\/p>\n

    <\/p>\n

    \u6b63\u5e38\u60c5\u6cc1\u4e0b\uff0c\u751f\u7269\u9ad4\u4e2d\u5929\u7136\u7684\u6297\u6c27\u5316\u5291\u9632\u79a6\u7cfb\u7d71\u4e2d\u7684\u6297\u6c27\u5316\u9176\u53ef\u4ee5\u8207\u98f2\u98df\u6216\u85e5\u7269\u4e2d\u7684\u6297\u6c27\u5316\u5291\u5354\u540c\u4f5c\u7528\uff0c\u6e05\u9664\u904e\u6c27\u5316\u7269\u3002\u5176\u4e2d\u6700\u91cd\u8981\u7684\u6297\u6c27\u5316\u9175\u7d20\u5305\u62ec\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\uff0c\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\uff08GPx\uff09\u548c\u904e\u6c27\u5316\u6c2b\u9176\uff08Cat\uff09\u3002SOD\u8ca0\u8cac\u5c07\u8d85\u6c27\u9670\u96e2\u5b50\u6b67\u5316\u70ba\u904e\u6c27\u5316\u6c2b\uff0c\u800cCat\u548cGPxs\u5247\u53ef\u4ee5\u6e1b\u5c11\u904e\u6c27\u5316\u6c2b\uff0c\u963b\u6b62\u9ad8\u6bd2\u6027\u7fa5\u57fa\u81ea\u7531\u57fa\u7684\u7522\u751f\u3002\u91cd\u8981\u7684\u662f\uff0cGPxs\u9084\u53ef\u4ee5\u6e1b\u5c11\u591a\u5143\u4e0d\u98fd\u548c\u8102\u80aa\u9178\u7684\u6c2b\u904e\u6c27\u5316\u7269\uff0c\u62b5\u6d88\u8102\u8cea\u904e\u6c27\u5316\u7684\u6bd2\u6027\u4f5c\u7528\u3002<\/p>\n

    <\/p>\n

    \u540c\u6642\u56e0\u70ba\u8102\u8cea\u904e\u6c27\u5316\u662f\u7531\u81ea\u7531\u57fa\u555f\u52d5\u7684\uff0c\u4e0d\u98fd\u548c\u8102\u80aa\u9178\u5728\u81ea\u7531\u57fa\u548c\u4e00\u4e9b\u6c27\u5316\u5f15\u767c\u8102\u985e\u6c27\u5316\u7684\u6700\u521d\u968e\u6bb5\u5f62\u6210\u5171\u8edb\u4e8c\u70ef, \u7136\u5f8c\u5f62\u6210\u74b0\u72c0\u904e\u6c27\u5316\u7269, \u6700\u5f8c\u7684\u4e3b\u8981\u7522\u7269\u662f\u4e19\u4e8c\u919b\uff08MDA\uff09\u3002<\/p>\n

    <\/p>\n

    \u6240\u4ee5\uff0c\u5224\u65b7\u6a5f\u9ad4\u6216\u7d30\u80de\u7684\u8102\u8cea\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6\uff0c\u4e00\u65b9\u9762\u9700\u8981\u6aa2\u6e2c\u6297\u6c27\u5316\u9176\uff08SOD\u3001CAT\u3001GPx\uff09\u6d3b\u529b\u4ee5\u5224\u65b7\u85e5\u7269\u7b49\u6e05\u9664\u6c27\u81ea\u7531\u57fa\u7684\u80fd\u529b\uff0c\u53e6\u4e00\u65b9\u9762\u6aa2\u6e2c\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269MDA\u7684\u6c34\u5e73\u4ee5\u9593\u63a5\u5224\u65b7\u7d30\u80de\u53d7\u81ea\u7531\u57fa\u653b\u64ca\u7684\u56b4\u91cd\u7a0b\u5ea6\u3002\u540c\u6642\u5206\u6790SOD\u3001CAT\u3001GPx\u3001MDA\u7684\u6c34\u5e73\u7d50\u679c\u5206\u6790\u53ef\u66f4\u5168\u9762\u8a55\u4f30\u6c27\u5316\u58d3\u529b\u6c34\u5e73\u7684\u8b8a\u5316\u3002<\/p>\n

    Abbkine \u53ef\u63d0\u4f9b\u5168\u5957\u8102\u8cea\u6c27\u5316\u640d\u50b7\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u6aa2\u6e2c\u65b9\u6cd5\u7c21\u55ae\u3001\u65b9\u4fbf\u3001\u9748\u654f\uff0c\u7522\u54c1\u8a73\u60c5\u5982\u4e0b\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n
    \u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u6aa2\u6e2c\u65b9\u6cd5<\/strong><\/td>\n\u9748\u654f\u5ea6<\/strong>&<\/strong>\u6aa2\u6e2c\u7bc4\u570d<\/strong><\/td>\n\u9069\u5408\u6a23\u672c<\/strong><\/td>\n\u8ca8\u865f<\/strong><\/td>\n<\/tr>\n
    CheKine\u2122 \u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176(SOD) \u6d3b\u529b\u6aa2\u6e2c\u8a66\u5291\u76d2<\/td>\n\u8f03\u7a69\u5b9a\/\u9748\u654f\u7684WST-8\u986f\u8272\u6cd5<\/td>\n3.13U\/ml -200U\/ml<\/td>\n\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2<\/td>\nKTB1030<\/a><\/td>\n<\/tr>\n
    \n

    CheKine\u2122 Catalase (CAT)<\/p>\n

    \u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/td>\n

    		\u76f4\u63a5\u5229\u7528\u904e\u6c27\u5316\u6c2b\u9176\u7684\u904e\u6c27\u5316\u529f\u80fd\u76f4\u63a5\u6e2c\u5b9a\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027<\/td>\n\u9748\u654f\u5ea6\u4f4e\u81f32 U\/ml<\/td>\n\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d05\u8840\u7403\u88c2\u89e3\u7522\u7269\u3001\u7d44\u7e54\u52fb\u6f3f\u3001\u7d30\u80de\u88c2\u89e3\u6db2\u7b49\u751f\u7269\u6a23\u54c1<\/td>\nKTB1040<\/a><\/td>\n<\/tr>\n
    CheKine\u2122\u8102\u8cea\u904e\u6c27\u5316\uff08MDA\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2<\/td>\n\u6539\u826f\u7684\u786b\u4ee3\u5df4\u6bd4\u59a5\u9178(TBA)\u6cd5<\/td>\n1-100 \u00b5M\/L<\/td>\n\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u5c3f\u6db2\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2<\/td>\nKTB1050<\/a><\/td>\n<\/tr>\n
    \n

    CheKine\u2122\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\uff08GSH-Px\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2<\/p>\n

     <\/td>\n

    		\u76f4\u63a5\u5229\u7528GSH-Px\u9176\u50ac\u5316H\u00a02<\/sub>\u00a0O\u00a02<\/sub>\u7684\u6d3b\u6027\u9032\u884c\u6aa2\u6e2c<\/td>\n\/\/<\/td>\n\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2<\/td>\nKTB1640<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u76f8\u95dc\u767c\u8868\u7684\u6587\u737b\uff1a<\/strong><\/span><\/p>\n

    Anton Bahtiar*, Putri Sagita Utami\uff0cetc. The Antioxidant Effects of the Ethanolic Extract of Binahong Leaves Unilateral Ureteral Obstruction Rat Model\uff0cPharmacogn J. DOI : 10.5530\/pj.2021.13.26.<\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Superoxide dismutase Activity Ass […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[1503],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25184"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25184"}],"version-history":[{"count":27,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25184\/revisions"}],"predecessor-version":[{"id":54575,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25184\/revisions\/54575"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25184"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25184"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25184"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}