{"id":25149,"date":"2020-06-02T15:39:47","date_gmt":"2020-06-02T07:39:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25149"},"modified":"2022-07-19T17:00:44","modified_gmt":"2022-07-19T09:00:44","slug":"hsp70-ab%e7%86%b1%e4%bc%91%e5%85%8b%e8%9b%8b%e7%99%bd70%e6%8a%97%e9%ab%94elisa%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fkte62472","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25149","title":{"rendered":"HSP70-Ab\u71b1\u4f11\u514b\u86cb\u767d70\u6297\u9ad4ELISA\u8a66\u5291\u76d2| \u8ca8\u865fKTE62472"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=25149\">HSP70-Ab\u71b1\u4f11\u514b\u86cb\u767d70\u6297\u9ad4ELISA\u8a66\u5291\u76d2<strong>| <\/strong><strong>\u8ca8\u865f<\/strong>KTE62472<\/a><\/span><\/p>\n<p>Human Heat Shock Protein 70 antibody (HSP70-Ab) ELISA Kit<\/p>\n<p>\u71b1\u4f11\u514b\u86cb\u767d70\uff08Heat shock protein 70, HSP70\uff09\u5728\u8a31\u591a\u985e\u578b\u7684\u816b\u7624(tumors)\u4e2d\u5747\u904e\u8868\u9054(overexpressed)\uff0c\u4e26\u88ab\u8a8d\u70ba\u8207\u816b\u7624\u7684\u9032\u5c55(tumor progression)\u5bc6\u5207\u76f8\u95dc\u3002\u96deHSP70\uff08chHSP70\uff09\u7570\u7a2eDNA\u75ab\u82d7(xenogeneic DNA vaccine)\uff0c\u53ef\u5728CTVT\uff08beagle canine transmissible venereal tumor , CTVT\uff09\u6a21\u578b\u4e2d\u5f15\u767c\u514d\u75ab\u61c9\u7b54\u3002 CTVT\u72ac\u63a5\u53d7\u7a7a\u8f09\u9ad4\u75ab\u82d7\u63a5\u7a2e\u4e14\u672a\u9032\u884c\u4efb\u4f55\u6cbb\u7642\uff08CW\uff09\u4f5c\u70ba\u5c0d\u7167\u3002\u7d50\u8ad6\u662f\uff0c\u7570\u7a2echHSP70 DNA\u75ab\u82d7\u5148\u5f8c\u7834\u58de\u4e86\u5c0d\u72acHSP70\u7684\u816b\u7624\u8010\u53d7\u6027\uff0c\u4e26\u8868\u73fe\u51fa\u6709\u5e0c\u671b\u7684\u9810\u9632\u6d3b\u6027\uff0c\u5f9e\u800c\u6291\u5236\u4e86\u8868\u9054HSP70\u7684\u816b\u7624\u7684\u751f\u9577\u3002 70 kilodalton\u7684\u71b1\u6fc0\u86cb\u767d\uff08Hsp70s\uff09\u662f\u4e00\u500b\u666e\u904d\u8868\u9054\u7684\u71b1\u6fc0\u86cb\u767d\u5bb6\u65cf(ubiquitously expressed heat shock proteins)\u3002\u5e7e\u4e4e\u6240\u6709\u6d3b\u751f\u7269\u9ad4\u4e2d\u90fd\u5b58\u5728\u5177\u6709\u76f8\u4f3c\u7d50\u69cb\u7684\u86cb\u767d\u8cea\u3002 Hsp70s\u662f\u7d30\u80de\u6298\u758a\u86cb\u767d\u8cea\u6a5f\u5236\u7684\u91cd\u8981\u7d44\u6210\u90e8\u5206\uff0c\u6709\u52a9\u65bc\u4fdd\u8b77\u7d30\u80de\u514d\u53d7\u58d3\u529b\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/www.abbkine.com\/file\/img\/KTE62472.png\" alt=\"\" width=\"499\" height=\"300\" \/><\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong>\u8a72\u4eba\u985e\u71b1\u6fc0\u86cb\u767d70\u6297\u9ad4\uff08HSP70-Ab\uff09ELISA\u8a66\u5291\u76d2 (<strong>\u8ca8\u865f<\/strong>KTE62472)\u63a1\u7528\u4e86\u7af6\u722d\u6027\u9176\u514d\u75ab\u5206\u6790\u6280\u8853\u3002\u8a72\u8a66\u5291\u76d2\u4e2d\u63d0\u4f9b\u7684\u5fae\u91cf\u6ef4\u5b9a\u677f\u5df2\u9810\u5148\u5857\u6709\u5c0dHSP70-Ab\u7279\u7570\u7684\u6297\u9ad4\u3002\u7136\u5f8c\u5c07\u6a19\u6e96\u54c1\u6216\u6a23\u54c1\u8207\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08HRP\uff09\u5171\u8edb\u7684HSP70-Ab\u52a0\u5165\u9069\u7576\u7684\u5fae\u91cf\u6ef4\u5b9a\u677f\u5b54\u4e2d\u4e26\u9032\u884c\u5b75\u80b2\u3002\u5728\u5177\u6709\u6297\u9ad4\u7684HRP\u6a19\u8a18\u7684HSP70-Ab\u548c\u672a\u6a19\u8a18\u7684HSP70-Ab\u4e4b\u9593\u555f\u52d5\u7af6\u722d\u6027\u6291\u5236\u53cd\u61c9\u3002\u5c07\u5e95\u7269\u6eb6\u6db2\u6dfb\u52a0\u5230\u5b54\u4e2d\uff0c\u4e26\u4e14\u984f\u8272\u8207\u6a23\u54c1\u4e2dHSP70-Ab\u7684\u6578\u91cf\u76f8\u53cd\u3002\u505c\u6b62\u986f\u8272\u4e26\u6e2c\u91cf\u984f\u8272\u5f37\u5ea6\u3002Detection range is 30pg\/ml &#8211; 480pg\/ml\u3002limit of detection value is 1 pg\/ml\u3002\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: Cell culture supernatants, Other biological fluids, Plasma, Serum<\/p>\n<ul>\n<li><strong>Product name<\/strong><\/li>\n<\/ul>\n<p>Human Heat Shock Protein 70 antibody (HSP70-Ab) ELISA Kit<\/p>\n<ul>\n<li><strong>Reactivity<\/strong><\/li>\n<\/ul>\n<p>Human<\/p>\n<ul>\n<li><strong>Applications<\/strong><\/li>\n<\/ul>\n<p>ELISA<\/p>\n<ul>\n<li><strong>Application notes<\/strong><\/li>\n<\/ul>\n<p>This Human Heat Shock Protein 70 antibody (HSP70-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP70-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated HSP70-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled HSP70-Ab and unlabeled HSP70-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of HSP70-Ab in the sample. The color development is stopped and the intensity of the color is measured.<\/p>\n<ul>\n<li><strong>Detection method<\/strong><\/li>\n<\/ul>\n<p>Colorimetric<\/p>\n<ul>\n<li><strong>Sample type<\/strong><\/li>\n<\/ul>\n<p>Cell culture supernatants, Other biological fluids, Plasma, Serum<\/p>\n<ul>\n<li><strong>Assay type<\/strong><\/li>\n<\/ul>\n<p>Sandwich ELISA (quantitative)<\/p>\n<ul>\n<li><strong>Assay duration<\/strong><\/li>\n<\/ul>\n<p>Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.<\/p>\n<ul>\n<li><strong>Kit components<\/strong><\/li>\n<li>Human Heat Shock Protein 70 antibody microplate<br \/>\n\u2022 Human Heat Shock Protein 70 antibody standard<br \/>\n\u2022 Human Heat Shock Protein 70 antibody detect antibody<br \/>\n\u2022 Streptavidin-HRP<br \/>\n\u2022 Standard diluent<br \/>\n\u2022 Assay buffer<br \/>\n\u2022 HRP substrate<br \/>\n\u2022 Stop solution<br \/>\n\u2022 Wash buffer<br \/>\n\u2022 Plate covers<\/li>\n<li><strong>Features &amp; Benefits<\/strong><\/li>\n<\/ul>\n<p>Human Heat Shock Protein 70 antibody (HSP70-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human HSP70-Ab. No significant cross-reactivity or interference between Human HSP70-Ab and analogues was observed.<\/p>\n<p>\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805<\/p>\n<ul>\n<li>\u8acb\u52ff\u6df7\u7528\u4e0d\u540c\u8a66\u5291\u76d2\u4e2d\u7684\u7d44\u5206\uff0c\u6216\u5728\u8a66\u5291\u76d2\u5230\u671f\u65e5\u671f\u4e4b\u5f8c\u4f7f\u7528\u8a66\u5291\u3002<\/li>\n<li>\u6253\u958b\u4e4b\u524d\uff0c\u8b93\u6240\u6709\u8a66\u5291\u81f3\u5c11\u52a0\u5ba4\u6eab30\u5206\u9418\u3002<\/li>\n<li>\u7528\u8a66\u5291\u9810\u6e05\u6d17\u79fb\u6db2\u5668\u5438\u982d\uff0c\u70ba\u6bcf\u500b\u6a23\u54c1\uff0c\u6a19\u6e96\u6db2\u548c\u8a66\u5291\u4f7f\u7528\u65b0\u9bae\u7684\u79fb\u6db2\u5668\u5438\u982d\uff0c\u4ee5\u514d\u6c61\u67d3\u3002<\/li>\n<li>\u672a\u4f7f\u7528\u7684\u5b54\u5fc5\u9808\u5728\u63d0\u4f9b\u7684\u5bc6\u5c01\u888b\u4e2d\u65bc4\u00b0C\u4e7e\u71e5\u3002<\/li>\n<li>\u5fb9\u5e95\u6df7\u5408\u5c0d\u65bc\u7d50\u679c\u975e\u5e38\u91cd\u8981\u3002\u5efa\u8b70\u6bcf10\u5206\u9418\u4f7f\u7528\u4f4e\u983b\u632f\u76ea\u5668\u6216\u7a0d\u7a0d\u6416\u52d5\u624b\u3002<\/li>\n<li>\u5efa\u8b70\u6240\u6709\u6a23\u54c1\u548c\u6a19\u6e96\u54c1\u4e00\u5f0f\u5169\u4efd\u6216\u4e00\u5f0f\u4e09\u4efd\u9032\u884c\u6e2c\u5b9a\u3002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>HSP70-Ab\u71b1\u4f11\u514b\u86cb\u767d70\u6297\u9ad4ELISA\u8a66\u5291\u76d2| \u8ca8\u865fKTE62472 Human Heat Shock  [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":24540,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452],"tags":[1509],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25149"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25149"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25149\/revisions"}],"predecessor-version":[{"id":25159,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25149\/revisions\/25159"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24540"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25149"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25149"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}