{"id":25083,"date":"2020-05-31T19:25:56","date_gmt":"2020-05-31T11:25:56","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25083"},"modified":"2022-07-19T17:01:17","modified_gmt":"2022-07-19T09:01:17","slug":"anti-epcam-aua1%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9fab00609-1-1","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25083","title":{"rendered":"Anti-EpCAM [AUA1]\u6297\u9ad4 |\u8ca8\u865fAb00609-1.1"},"content":{"rendered":"

Anti-EpCAM [AUA1]\u6297\u9ad4 |\u8ca8\u865fAb00609-1.1\u00a0<\/a><\/span><\/p>\n

EpCAM\u53ef\u4f5c\u70ba\u8178\u9053\u4e0a\u76ae\u7d30\u80de(intestinal epithelial cells , IECs)\u548c\u5167\u76ae\u6dcb\u5df4\u7d30\u80de( intraepithelial lymphocytes , IEL)\u4e4b\u9593\u7684\u7269\u7406\u540c\u7fa9\u76f8\u4e92\u4f5c\u7528\u5206\u5b50,\u5728\u7c98\u819c\u4e0a\u76ae\u4e0a\u76ae\u63d0\u4f9b\u514d\u75ab\u5c4f\u969c\u4f5c\u70ba\u62b5\u79a6\u7c98\u819c\u611f\u67d3\u7684\u7b2c\u4e00\u9053\u9632\u7dda\u3002\u5728\u80da\u80ce\u5e79\u7d30\u80de( embryonic stem cells) \u589e\u6b96(proliferation)\u548c\u5206\u5316( differentiation)\u4e2d\u767c\u63ee\u4f5c\u7528\u3002\u5411\u4e0a\u8abf\u7bc0 FABP5\u3001MYC \u548ccyclins A \u548c cyclins E \u7684\u8868\u9054\u3002<\/p>\n

\"Binding<\/p>\n

Immunofluorescence staining of fixed Caco-2 cells with anti-EpCAM antibody AUA1 (Ab00609)\u00a0<\/b>Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix\u2122 coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of AUA1 (Ab00609-1.1) at 10 \u00b5g\/ml for 1h followed by Alexa Fluor\u00ae\u00a0488 secondary antibody (2 \u00b5g\/ml), showing strong membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00609-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Ab00178-1.1) followed by Alexa Fluor\u00ae\u00a0488 secondary antibody.<\/p>\n

\u6297\u9ad4\u4ecb\u7d39<\/strong>:<\/strong>\u00a0\u00a0Anti-EpCAM [AUA1]\u6297\u9ad4\u5c0dEpCAM\u7684\u7d50\u5408\u7279\u7570\u6027 (Specificity)\u3002Species Reactivity: Human\u3002\u6b64\u6297\u9ad4\u53ef\u61c9\u7528\u65bcFACS, ELISA, IF, IHC\u7b49\u5be6\u9a57\u3002Alternative Name(s) of Target: CD326; Epithelial Cell Adhesion Molecule\u3002\u514d\u75ab\u539f(Immunogen: LoVo cell line, a colon carcinoma cell line)\u3002Isotype and Format: Mouse IgG1, Kappa Clone Number\u3002AUA1\u61c9\u7528\u8aaa\u660e\uff1aAUA1\u8207\u4e73\u817a\u764c(breast carcinomas)\uff0c\u814e\u5c0f\u7ba1(kidney tubules)\uff0c\u6241\u6843\u9ad4\u96b1\u7aa9\u4e0a\u76ae(tonsil crypt epithelium)\u548c\u7d30\u80de\u767c\u751f\u56b4\u91cd\u53cd\u61c9(serious effusions)\u6642\u6703\u767c\u751f\u4ea4\u53c9\u53cd\u61c9(cross reacts)\u3002\u9019\u662f\u7528\u65bc\u8868\u5fb5\u6240\u6709\u4e0a\u76ae\u7d30\u80de(epithelial cells)\u7684\u826f\u597d\u6297\u9ad4\u3002\u6297\u9ad4\u5177\u6709Alkaline phoshatase\u3001Allophycocyanin\u3001APC\/Cy7\u3001Atto488\u3001Biotin Type A\u3001Biotin Type B\u3001FluoProbes647H\u3001Fluorescein\u3001HRP\u3001PE\/CY7\u3001R-Phycoerythrin\u3001Streptavidin\u7b49\u4e0d\u540c\u67d3\u5291\u4e4b\u6a19\u8a8c\u3002\u5728\u9078\u8cfc\u6642\u8acb\u8a3b\u660e\u6a19\u8a8c\uff0c\u82e5\u672a\u9078\u64c7\u5247\u70ba\u672a\u6a19\u8a8c\u5305\u88dd\u3002<\/p>\n

 <\/p>\n

Purified antibody.<\/p>\n

Purification:<\/strong>\u00a0Protein A affinity purified<\/p>\n

Supplied in:<\/strong>\u00a0PBS with 0.02% Proclin 300.<\/p>\n

Storage Recommendation:<\/strong>\u00a0Store at 4\u2070C for up to 3 months. For longer storage, aliquot and store at -20\u2070C.<\/p>\n

Concentration:<\/strong>\u00a01 mg\/ml.<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\nMouse target<\/td>\n\u6297\u9ad4clone<\/td>\nOriginal Antibody format<\/td>\n<\/tr>\n
Ab01052<\/a><\/td>\n4-1BB (CD137)<\/td>\nLOB12.3<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n<\/thead>\n
Ab01054<\/a><\/td>\n4-1BBL (CD137L)<\/td>\nAT113-2<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00881<\/a><\/td>\n4-1BBL (CD137L)<\/td>\nTKS-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01027<\/a><\/td>\nBTLA<\/td>\nHMBT-6B2 <\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00670<\/a><\/td>\nCD27<\/td>\nLG.3A10<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00895<\/a><\/td>\nCD27<\/td>\nRM27-3E5 <\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00286<\/a><\/td>\nCD28<\/td>\nE18 <\/a><\/td>\nMouse IgG2b<\/td>\n<\/tr>\n
Ab00285<\/a><\/td>\nCD28<\/td>\nD665 <\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01109<\/a><\/td>\nCD40<\/td>\n3\/23 <\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01087<\/a><\/td>\nCD40L (CD154)<\/td>\nMR1 <\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00816<\/a><\/td>\nCD70<\/td>\nTAN 1-7 <\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01056<\/a><\/td>\nCD70<\/td>\nTAN1-6 <\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01028<\/td>\nCD80 (B7-1)<\/td>\nRM80<\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01029<\/a><\/td>\nCD86 (B7-2)<\/td>\nPO.3<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab01089<\/a><\/td>\nCD153<\/td>\nRM153<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab01297<\/a><\/td>\nCD96<\/td>\n6A6<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00894<\/a><\/td>\nCTLA-4<\/td>\n9H10<\/a><\/td>\nHamster IgG2<\/td>\n<\/tr>\n
Ab01018<\/a><\/td>\nCTLA-4<\/td>\n9D9<\/a><\/td>\nMouse IgG2b<\/td>\n<\/tr>\n
Ab01088<\/a><\/td>\nGAL-9<\/td>\nRG9-35<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01060<\/a><\/td>\nGITR<\/td>\nDTA-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00374<\/a><\/td>\nGITR<\/td>\nYGITR 860.103.5<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00631<\/a><\/td>\nGITR<\/td>\nYGITR765<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00630<\/a><\/td>\nGITRL<\/td>\nYGL386<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01026<\/a><\/td>\nHVEM<\/td>\nHMHV-1B18<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00814<\/a><\/td>\nICOS<\/td>\n7E.17G9<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00882<\/a><\/td>\nICOSL<\/td>\nHK5.3<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01295<\/a><\/td>\nLAG3<\/td>\nC9B7W<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01295<\/a><\/td>\nLAG3<\/td>\nC9B7W<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00110<\/a><\/td>\nOX40 (CD134)<\/td>\nOX86<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00564<\/a><\/td>\nOX40L<\/td>\nOX-89<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00813<\/a><\/td>\nPD-1<\/td>\nRMP1-14<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01017<\/a><\/td>\nPD-1H (VISTA)<\/td>\nmam82<\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01016<\/a><\/td>\nPD-1H (VISTA)<\/td>\nMH5A<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00373<\/a><\/td>\nPD-L1<\/td>\nYDC 127.1.1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00883<\/a><\/td>\nPD-L2<\/td>\nTY25<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00875<\/a><\/td>\nPDPN (MAP-Tag)<\/td>\nPMab-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00973<\/a><\/td>\nTIGIT<\/td>\n4D4<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab01258<\/a><\/td>\nTIGIT<\/td>\n1B4<\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01032<\/a><\/td>\nTIM-1<\/td>\n3B3<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01110<\/a><\/td>\nTIM-2<\/td>\nRMT2-14<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01057<\/a><\/td>\nTIM-3<\/td>\n2C12<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01058<\/a><\/td>\nTIM-4<\/td>\n5G3<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01059<\/a><\/td>\nTIM-4<\/td>\n3A1<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
 <\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Anti-EpCAM [AUA1]\u6297\u9ad4 |\u8ca8\u865fAb00609-1.1\u00a0 EpCAM\u53ef\u4f5c\u70ba\u8178\u9053\u4e0a\u76ae\u7d30\u80de(inte […]<\/p>\n","protected":false},"author":3,"featured_media":25084,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[459],"tags":[1513],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25083"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25083"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25083\/revisions"}],"predecessor-version":[{"id":25085,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25083\/revisions\/25085"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/25084"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25083"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25083"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25083"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}