{"id":24999,"date":"2020-05-27T20:34:56","date_gmt":"2020-05-27T12:34:56","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24999"},"modified":"2025-12-01T17:15:30","modified_gmt":"2025-12-01T09:15:30","slug":"%e6%b4%bb%e7%b4%b0%e8%83%9e%e5%92%8c%e6%ad%bb%e7%b4%b0%e8%83%9e%e9%9b%99%e6%9f%93%e8%89%b2%e5%a5%97%e7%b5%84-live-and-dead-cell-double-staining-kit%e8%b2%a8%e8%99%9fkta1001","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=24999","title":{"rendered":"\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44 | Live and Dead Cell Double Staining Kit\u8ca8\u865fKTA1001"},"content":{"rendered":"

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44 | Live and Dead Cell Double Staining Kit\u8ca8\u865fKTA1001<\/a><\/span><\/strong><\/span><\/p>\n

Detect using either microscopy or FACS.<\/p>\n

\u7d30\u80de\u4ecb\u5c0e\u7d30\u80de\u6bd2\u6027(Cell-mediated cytotoxicity)\u662f\u514d\u75ab\u7cfb\u7d71(immune system) \u5c0d\u9ad4\u5167\u53d7\u640d\u7d30\u80de\uff0ccytolysis\u7684\u4e00\u500b\u91cd\u8981\u73fe\u8c61\u3002\u5340\u5206\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u5c0d\u65bc\u7814\u7a76\u751f\u9577\u63a7\u5236\u548c\u7d30\u80de\u6b7b\u4ea1\u5177\u91cd\u8981\u6027\u3002Abbkine \u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2 (Abbkine Live and Dead Cell Double Staining Kit) \u63d0\u4f9b\u4e86\u4e00\u500b\u65b9\u4fbf\u7684\u6e2c\u5b9a,\u9032\u884c\u8a55\u4f30\u7d30\u80de\u7684\u53ef\u884c\u6027\uff1b\u5957\u7d44\u63d0\u4f9b\u5169\u7a2eprobes\u540c\u6642\u6e2c\u5b9a\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de, \u53ef\u7528\u65bc\u6e2c\u91cf\u7d30\u80de\u5065\u5eb7\u7684\u8b58\u5225\u53c3\u6578:\u8840\u6f3f\u819c\u5b8c\u6574\u6027(plasma membrane integrity) \u548c\u7d30\u80de\u5167\u916f\u9176\u6d3b\u6027(intracellular esterase activity)\u3002\u8a72\u8a66\u5291\u76d2\u5229\u7528LiveDye\u6d3b\u7d30\u80de\u67d3\u5291; \u4e00\u7a2e\u7d30\u80de\u6ef2\u900f\u7684\u7da0\u8272\u87a2\u5149\u67d3\u6599(cell-permeable green fluorescent dye , Ex\/Em = 488\/530 nm)\u67d3\u8272\u6d3b\u7d30\u80de(live cells)\u3002NucleiDye\u6838\u67d3\u6599,\u70ba\u4e00\u7a2e\u7d30\u80de\u4e0d\u53ef\u6ef2\u900f\u7684\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617) , \u7528\u65bc\u67d3\u8272\u6b7b\u7d30\u80de(dead cells)\u3002<\/p>\n

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\nAbbkine \u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2 (Live and Dead Cell Double Staining Kit, KTA1001) \u63d0\u4f9b\u4e86\u4e00\u500b\u65b9\u4fbf\u7684\u6e2c\u5b9a\u8a55\u4f30\u6b7b\u6d3b\u7d30\u80de\u7684\u5feb\u901f\u65b9\u6cd5,\u57fa\u65bc\u540c\u6642\u6e2c\u5b9a\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u8207\u5169\u7a2eProbe\u3002\u6e2c\u91cf\u7d30\u80de\u5065\u5eb7\u3001\u8840\u6f3f\u819c\u5b8c\u6574\u6027\u3001\u548c\u7d30\u80de\u5167\u916f\u9176\u6d3b\u6027\u3002\u8a72\u8a66\u5291\u76d2\u5229\u7528LiveDye\u4e00\u7a2e\u7d30\u80de\u6ef2\u900f\u7684\u7da0\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 488\/530 nm)\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u6838\u67d3\u6599\uff1bNucleiDye\u4e00\u7a2e\u7d30\u80de\u4e0d\u53ef\u6ef2\u900f\u7684\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)\u4ee5\u67d3\u8272\u6b7b\u7d30\u80de\u3002<\/p>\n

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<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nLive and Dead Cell Double Staining Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nAbbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. The kit utilizes LiveDye, a cell-permeable green fluorescent dye (Ex\/Em = 488\/530 nm), to stain live cells and NucleiDye, a cell non-permeable red fluorescent dye (Ex\/Em = 535\/617), to stain dead cells.<\/td>\n<\/tr>\n
it components<\/strong><\/td>\n\u2022 LiveDye
\n\u2022 NucleiDye
\n\u2022 Assay Buffer (10X)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Suitable for mammalian cells staining.
\n\u2022 Useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy.
\n\u2022 LiveDye (green, Ex\/Em=488\/530nm), NucleiDye (red, Ex\/Em= 535\/617nm).<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\nNucleiDye is a potential mutagen. Use appropriate precautions when handling this reagent.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nRefer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nCell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Distinguishing between live and dead cells is very important for investigation of growth control and cell death.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

1. Ocular surface repair using decellularized porcine conjunctiva
\nL Zhao, Y Jia, C Zhao. Acta biomaterialia, 2019 – Elsevier
\n2. Grafting antibacterial polymer brushes from titanium surface via polydopamine chemistry and activators regenerated by electron transfer ATRP
\nShuangshuang Wang, Jixuan Song, Yuchao Li, et al. Reactive and Functional Polymers. July 2019, 140: 48-55.<\/p>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n

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\"\"<\/strong><\/span>\u7d30\u80de\u589e\u6b96 \/ \u7d30\u80de\u6bd2\u6027 \/ \u7d30\u80de\u8870\u8001<\/span><\/h3>\n<\/div>\n