\u6297\u9ad4\u4ecb\u7d39: \u6b64\u6297\u9ad4\u7279\u7570\u6027\u91dd\u5c0dPD-1H\u6297\u539f\u3002The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL\/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-\u03bcg dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011).\u3002\u53ef\u61c9\u7528\u65bctherapeutic, Block, ELISA, IHC\u7b49\u5be6\u9a57\u3002Species Reactivity: Mouse<\/p>\n
![\"Binding](\"https:\/\/absoluteantibody.com\/wp-content\/uploads\/2018\/10\/IMG00456Ab01016-23.0IFfull.png\")
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Immunofluorescence staining of fixed mouse splenocytes with anti-PD-1H antibody MH5A (Ab01016) <\/b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of MH5A (Ab01016-23.0) at 10 \u00b5g\/ml for 1h followed by Alexa Fluor\u00ae\u00a0488 secondary antibody (2 \u00b5g\/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01016-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (Ab178-23.0) followed by Alexa Fluor\u00ae\u00a0488 secondary antibody.<\/p>\n
![\"Binding](\"https:\/\/absoluteantibody.com\/wp-content\/uploads\/2019\/05\/IMG00471Ab01016-23.0ELISAfull.png\")
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ELISA of anti-VISTA antibody on VISTA-Fc fusion protein.<\/b> Binding curve of the rabbit chimeric version of the anti-VISTA antibody MH5A (Ab01016-23.0; blue line) to an ELISA plate coated with mouse VISTA-Fc fusion protein (Pr00164-1.9) at a concentration of 5 \u00b5g\/ml. A 3-fold serial dilution from 10000 to 1.7 ng\/ml was performed using Ab01016-23.0. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP (BioRad) antibody was used.<\/p>\n
Purified antibody.
\nPurification: Protein A affinity purified
\nSupplied in: PBS with 0.02% Proclin 300.
\nStorage Recommendation: Store at 4\u2070C for up to 3 months. For longer storage, aliquot and store at -20\u2070C.
\nConcentration: 1 mg\/ml.<\/p>\n
UniProt Accession Number of Target Protein:<\/strong> Q9D659<\/p>\n Alternative Name(s) of Target:<\/strong> VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set immunoregulatory receptor Specificity:<\/strong> This antibody specifically binds PD-1H.<\/p>\n Application Notes:<\/strong> The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL\/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-\u03bcg dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011).<\/p>\n Antibody first published in:<\/strong> Flies D et al. Cutting Edge: A Monoclonal Antibody Specific for the Programmed Death-1 Homolog Prevents Graft-versus-Host Disease in Mouse Models J Immunol August 15, 2011, 187 (4) 1537-1541 PMID:21768399<\/a> <\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" \u514d\u75ab\u7642\u6cd5\u7814\u7a76(Immunotherapy Research) \u6d3b\u9ad4\u7528\u91cd\u7d44\u6297\u9ad4 \u91dd\u5c0d\u6cbb\u7642\u7814\u7a76\uff0c\u6297\u86cb\u767d\u8cea\u7684\u6297\u9ad4\u53c3\u8207 […]<\/p>\n","protected":false},"author":3,"featured_media":3519,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1538,1305,1539,1304],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24830"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24830"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24830\/revisions"}],"predecessor-version":[{"id":24838,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24830\/revisions\/24838"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/3519"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24830"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24830"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24830"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nImmunogen:<\/strong> The antibody was generated by immunising Armenian hamsters with mPD-1HIg.<\/p>\n
\nNote on publication:<\/strong> Describes the generation of the antibody and characterisation of its theraputic function in models of graft-versus-host disease.<\/p>\n