Anti-alpha V beta 8 integrin [EM13309], Mouse IgG1, Kappa\u8ca8\u865fAb00892-1.1 \u00a0<\/strong><\/a><\/span><\/p>\n Immunohistochemical staining of various human tumour cell lines using anti-alpha-V beta 8 integrin antibody (Ab00892) EM13309 <\/b> Anti-alpha-V beta 8 integrin staining of formaldehyde fixed paraffin embedded Hs 852 T, IGROV-1 and HCC-827 cell line samples exhibiting varying degrees of target expression (level in parentheses). The rabbit-chimeric version of EM13309 (Ab00892-23.0) was used to stain samples at a concentration of 2 \u00b5g\/ml.<\/p>\n <\/p>\n Alternative Name(s) of Target:<\/strong> \u03b1v\u03b28; Vitronectin receptor subunit alpha; CD51; Integrin beta-8; ITGB8; P06756; P26012; avb8<\/p>\n Immunogen:<\/strong> This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas<\/p>\n Specificity:<\/strong> This antibody is specific for the\u00a0extracellular domain of human recombinant transmembrane-truncated (DTM) \u03b1v\u03b28 integrin, a dimeric cell surface protein that mediates cell attachment. \u03b1v\u03b28 integrin promotes the activation of latent TGF-\u03b2, as well as inhibiting tumour cell growth.<\/p>\n Application Notes:<\/strong> This antibody has been used to specifically bind \u03b1v\u03b28 in Western blots and in flow cytometry, and to specifically stain \u03b1v\u03b28 integrins in FFPE(formalin fixed paraffin embedded)\/TMA material, where murine antibodies function poorly (Goodman et al, 2012; He\u00df et al., 2014; Roth et al, 2013). \u03b1v\u03b28 is weakly expressed in human tumour specimens and non-small-cell lung carcinoma, with some expression in ovarian carcinoma and melanoma cells.<\/p>\n Antibody first published in:<\/strong> Goodman et al. Matched rabbit monoclonal antibodies against \u03b1v-series integrins reveal a novel \u03b1v\u03b23-LIBS epitope, and permit routine staining of archival paraffin samples of human tumours Biol Open. 2012 Apr 15; 1(4): 329\u2013340. PMID:23213423<\/a> <\/p>\n <\/p>\n <\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" \u514d\u75ab\u7642\u6cd5\u7814\u7a76(Immunotherapy Research) \u6d3b\u9ad4\u7528\u91cd\u7d44\u6297\u9ad4 \u91dd\u5c0d\u6cbb\u7642\u7814\u7a76\uff0c\u6297\u86cb\u767d\u8cea\u7684\u6297\u9ad4\u53c3\u8207 […]<\/p>\n","protected":false},"author":3,"featured_media":3519,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[1544],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24790"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24790"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24790\/revisions"}],"predecessor-version":[{"id":24793,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24790\/revisions\/24793"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/3519"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24790"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24790"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24790"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}![\"Binding](\"https:\/\/absoluteantibody.com\/wp-content\/uploads\/2018\/08\/IMG00297Ab00892-23.0IHCfull.png\")
<\/p>\n
\nNote on publication:<\/strong> Describes the generation and characterisation of this antibody.<\/p>\n\n\n
\n \u7522\u54c1\u8ca8\u865f<\/td>\n Mouse target<\/td>\n \u6297\u9ad4clone<\/td>\n Original Antibody format<\/td>\n<\/tr>\n \n \nAb01052<\/a><\/td>\n 4-1BB (CD137)<\/td>\n LOB12.3<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n<\/thead>\n \n Ab01054<\/a><\/td>\n 4-1BBL (CD137L)<\/td>\n AT113-2<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n \n Ab00881<\/a><\/td>\n 4-1BBL (CD137L)<\/td>\n TKS-1<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01027<\/a><\/td>\n BTLA<\/td>\n HMBT-6B2 <\/a><\/td>\n Hamster IgG<\/td>\n<\/tr>\n \n Ab00670<\/a><\/td>\n CD27<\/td>\n LG.3A10<\/a><\/td>\n Hamster IgG<\/td>\n<\/tr>\n \n Ab00895<\/a><\/td>\n CD27<\/td>\n RM27-3E5 <\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab00286<\/a><\/td>\n CD28<\/td>\n E18 <\/a><\/td>\n Mouse IgG2b<\/td>\n<\/tr>\n \n Ab00285<\/a><\/td>\n CD28<\/td>\n D665 <\/a><\/td>\n Mouse IgG1<\/td>\n<\/tr>\n \n Ab01109<\/a><\/td>\n CD40<\/td>\n 3\/23 <\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01087<\/a><\/td>\n CD40L (CD154)<\/td>\n MR1 <\/a><\/td>\n Hamster IgG<\/td>\n<\/tr>\n \n Ab00816<\/a><\/td>\n CD70<\/td>\n TAN 1-7 <\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01056<\/a><\/td>\n CD70<\/td>\n TAN1-6 <\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01028<\/td>\n CD80 (B7-1)<\/td>\n RM80<\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01029<\/a><\/td>\n CD86 (B7-2)<\/td>\n PO.3<\/a><\/td>\n Rat IgG2b<\/td>\n<\/tr>\n \n Ab01089<\/a><\/td>\n CD153<\/td>\n RM153<\/a><\/td>\n Rat IgG2b<\/td>\n<\/tr>\n \n Ab01297<\/a><\/td>\n CD96<\/td>\n 6A6<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab00894<\/a><\/td>\n CTLA-4<\/td>\n 9H10<\/a><\/td>\n Hamster IgG2<\/td>\n<\/tr>\n \n Ab01018<\/a><\/td>\n CTLA-4<\/td>\n 9D9<\/a><\/td>\n Mouse IgG2b<\/td>\n<\/tr>\n \n Ab01088<\/a><\/td>\n GAL-9<\/td>\n RG9-35<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01060<\/a><\/td>\n GITR<\/td>\n DTA-1<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab00374<\/a><\/td>\n GITR<\/td>\n YGITR 860.103.5<\/a><\/td>\n Rat IgG2b<\/td>\n<\/tr>\n \n Ab00631<\/a><\/td>\n GITR<\/td>\n YGITR765<\/a><\/td>\n Rat IgG2b<\/td>\n<\/tr>\n \n Ab00630<\/a><\/td>\n GITRL<\/td>\n YGL386<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n \n Ab01026<\/a><\/td>\n HVEM<\/td>\n HMHV-1B18<\/a><\/td>\n Hamster IgG<\/td>\n<\/tr>\n \n Ab00814<\/a><\/td>\n ICOS<\/td>\n 7E.17G9<\/a><\/td>\n Rat IgG2b<\/td>\n<\/tr>\n \n Ab00882<\/a><\/td>\n ICOSL<\/td>\n HK5.3<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01295<\/a><\/td>\n LAG3<\/td>\n C9B7W<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n \n Ab01295<\/a><\/td>\n LAG3<\/td>\n C9B7W<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n \n Ab00110<\/a><\/td>\n OX40 (CD134)<\/td>\n OX86<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n \n Ab00564<\/a><\/td>\n OX40L<\/td>\n OX-89<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n \n Ab00813<\/a><\/td>\n PD-1<\/td>\n RMP1-14<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01017<\/a><\/td>\n PD-1H (VISTA)<\/td>\n mam82<\/a><\/td>\n Mouse IgG1<\/td>\n<\/tr>\n \n Ab01016<\/a><\/td>\n PD-1H (VISTA)<\/td>\n MH5A<\/a><\/td>\n Hamster IgG<\/td>\n<\/tr>\n \n Ab00373<\/a><\/td>\n PD-L1<\/td>\n YDC 127.1.1<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab00883<\/a><\/td>\n PD-L2<\/td>\n TY25<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab00875<\/a><\/td>\n PDPN (MAP-Tag)<\/td>\n PMab-1<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab00973<\/a><\/td>\n TIGIT<\/td>\n 4D4<\/a><\/td>\n Hamster IgG<\/td>\n<\/tr>\n \n Ab01258<\/a><\/td>\n TIGIT<\/td>\n 1B4<\/a><\/td>\n Mouse IgG1<\/td>\n<\/tr>\n \n Ab01032<\/a><\/td>\n TIM-1<\/td>\n 3B3<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01110<\/a><\/td>\n TIM-2<\/td>\n RMT2-14<\/a><\/td>\n Rat IgG2a<\/td>\n<\/tr>\n \n Ab01057<\/a><\/td>\n TIM-3<\/td>\n 2C12<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n \n Ab01058<\/a><\/td>\n TIM-4<\/td>\n 5G3<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n \n Ab01059<\/a><\/td>\n TIM-4<\/td>\n 3A1<\/a><\/td>\n Rat IgG1<\/td>\n<\/tr>\n \n <\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n