{"id":24740,"date":"2020-05-15T16:24:15","date_gmt":"2020-05-15T08:24:15","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24740"},"modified":"2024-04-26T11:45:00","modified_gmt":"2024-04-26T03:45:00","slug":"jc-1%e7%b7%9a%e7%b2%92%e9%ab%94%e8%86%9c%e9%9b%bb%e4%bd%8d%e6%b8%ac%e5%ae%9a-mitochondrial-membrane-potential-assay-kit-%e8%b2%a8%e8%99%9fkta4001","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=24740","title":{"rendered":"JC-1\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a | Mitochondrial Membrane Potential Assay Kit \u8ca8\u865fKTA4001"},"content":{"rendered":"

JC-1<\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>CAT # KTA4001 (6well\/T)<\/a><\/span><\/p>\n

Mitochondrial Membrane Potential Assay Kit (JC-1)<\/p>\n

\"\"<\/p>\n

Fig. Hela cells stained with Abbkine Mitochondrial Membrane Potential Assay Kit (JC-1). A: Red fluorescence indicates healthy mitochondria, B: Green fluorescence indicates mitochondria in poor health (30min incubation in 20uM CCCP).<\/p>\n

Abbkine\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08Mitochondrial Membrane Potential Assay Kit , JC-1\u8ca8\u865fKTA4001\uff09\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u65b9\u6cd5\uff0c\u57fa\u65bc\u78b3\u83c1\u67d3\u6599(carbocyanine dye ) JC-1\u901a\u904e\u6aa2\u6e2c\u7dda\u7c92\u9ad4\u8de8\u819c\u96fb\u4f4d\u7684\u8b8a\u5316\u4f86\u5340\u5206\u5065\u5eb7\u7d30\u80de\u548c\u51cb\u4ea1\u7d30\u80de(apoptotic cells)\u3002\u5728\u5065\u5eb7\u7d30\u80de\u4e2d\uff0c\u9019\u7a2e\u67d3\u6599\u5728\u7c92\u817a\u9ad4\u4e2d\u7a4d\u7d2f(accumulates)\u548c\u805a\u96c6(aggregates)\uff0c\u5f62\u6210\u9bae\u7d05\u8272\u7684\u87a2\u5149\u5718\u584a\u3002\u8017\u6563\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u90fd\u6703\u963b\u6b62JC-1\u67d3\u6599\u5728\u7dda\u7c92\u9ad4\u4e2d\u7a4d\u805a\uff0c\u56e0\u6b64\u8a72\u67d3\u6599\u4ee5\u5176\u55ae\u9ad4\u5f62\u5f0f\u4fdd\u7559\u5728\u7d30\u80de\u8cea\u4e2d\uff0c\u5c0e\u81f4\u5f9e\u7d05\u8272\u7684\u8f49\u8b8a\uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09\u3002 JC-1\u53ef\u7528\u4f5c\u591a\u7a2e\u7d30\u80de\u985e\u578b\uff08\u5305\u62ec\u808c\u7d30\u80de\u548c\u795e\u7d93\u5143\uff09\u4ee5\u53ca\u5b8c\u6574\u7d44\u7e54\u548c\u5206\u96e2\u7684\u7c92\u817a\u9ad4\u4e2d\u7c92\u817a\u9ad4\u6f5b\u529b\u7684\u6307\u6a19\u3002\u8a72\u8a66\u5291\u76d2\u5305\u542bCCCP\uff0cCCCP\u5c0e\u81f4\u8cea\u5b50\u68af\u5ea6\u89e3\u5076\u806f\uff0c\u8a72\u8cea\u5b50\u68af\u5ea6\u5728\u96fb\u5b50\u50b3\u8f38\u93c8\u4e2d\u96fb\u5b50\u8f09\u9ad4\u7684\u6b63\u5e38\u6d3b\u52d5\u904e\u7a0b\u4e2d\u5efa\u7acb\uff0c\u4e26\u56e0\u6b64\u6d88\u9664\u4e86\u7dda\u7c92\u9ad4\u7684\u96fb\u5316\u5b78\u52e2\uff0c\u53ef\u4ee5\u7528\u4f5c\u9632\u6b62JC-1\u805a\u96c6\u7684\u5c0d\u7167\u7d44\u3002<\/p>\n

\"KTA4001\"<\/a><\/p>\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nMitochondrial Membrane Potential Assay Kit (JC-1)<\/a><\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 JC-1 Stain
\n\u2022 CCCP (10 mM)
\n\u2022 Assay Buffer (5\u00d7)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Measure mitochondrial membrane potential as an indicator of cell health.
\n\u2022 Use JC-1, a fluorescent, lipophilic, cationic dye.
\n\u2022 Red fluorescence indicates healthy mitochondria and green fluorescence indicates mitochondria in poor health.
\n\u2022 Optimized for flow cytometery or fluorescent microscopy.
\n\u2022 Contain CCC (Potent mitochondrial oxidative phosphorylation uncoupler).<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 JC-1 Stain is difficult to prepare due to its low solubility. Make sure JC-1 Stain is completely thawed and warmed to room temperature before using.
\n\u2022 JC-1 Stain is light senstive. Incubations need to be done in the dark.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nRefer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipmen<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u8ca8\u865fCatalog no<\/td>\n\u71b1\u9580\u5546\u54c1\u63a8\u85a6<\/td>\n<\/tr>\n
A21010<\/td>\nHRP, Goat Anti-Mouse IgG<\/td>\n<\/tr>\n
A21020<\/td>\nHRP, Goat Anti-Rabbit IgG<\/td>\n<\/tr>\n
A01050<\/td>\nAnti-Plant Actin Mouse Monoclonal Antibody (3T3)<\/td>\n<\/tr>\n
A23920<\/td>\nDylight 800, Goat Anti-Rabbit IgG<\/td>\n<\/tr>\n
A25112<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG HCS<\/td>\n<\/tr>\n
A23220<\/td>\nDylight 488, Goat Anti-Rabbit IgG<\/td>\n<\/tr>\n
A23420<\/td>\nDylight 594, Goat Anti-Rabbit IgG<\/td>\n<\/tr>\n
A25022<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG LCS<\/td>\n<\/tr>\n
KET6017<\/td>\nEliKine\u2122 Human IL-6 ELISA Kit<\/td>\n<\/tr>\n
A01090<\/td>\nAnti-Lamin B1 Monoclonal Antibody (15T1)<\/td>\n<\/tr>\n
A02010<\/td>\nAnti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n<\/tr>\n
A01030<\/td>\nAnti-\u03b2-Tubulin Mouse Monoclonal Antibody (3G6)<\/td>\n<\/tr>\n
A01060<\/td>\nAnti-COX IV Mouse Monoclonal Antibody (14Y2)<\/td>\n<\/tr>\n
A01070<\/td>\nAnti-Histone H3 Mouse Monoclonal Antibody (2D10)<\/td>\n<\/tr>\n
BMD00084<\/td>\nAbFluor\u2122 594-Phalloidin<\/td>\n<\/tr>\n
BMM3001<\/td>\nColorcode Prestained Protein Marker (10-180 kDa)<\/td>\n<\/tr>\n
BMP1001<\/td>\nProtease Inhibitor Cocktail (100X)<\/td>\n<\/tr>\n
A23310<\/td>\nDylight 549, Goat Anti-Mouse IgG<\/td>\n<\/tr>\n
BMR20100<\/td>\nPurKine\u2122 GST-Tag Glutathione Resin<\/td>\n<\/tr>\n
BMR20206<\/td>\nPurKine\u2122 MBP-Tag Dextrin Resin 6FF<\/td>\n<\/tr>\n
BMS0050<\/td>\nNormal Goat Serum<\/td>\n<\/tr>\n
A02020<\/td>\nAnti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n<\/tr>\n
A02040<\/td>\nAnti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n<\/tr>\n
A02050MGB<\/td>\nAnti-His Tag Mouse Monoclonal Antibody (5C3), Magnetic Beads<\/td>\n<\/tr>\n
A22110<\/td>\nFITC, Goat Anti-Mouse IgG<\/td>\n<\/tr>\n
A22120<\/td>\nFITC, Goat Anti-Rabbit IgG<\/td>\n<\/tr>\n
A23120<\/td>\nDyLight 405, Goat Anti-Rabbit IgG<\/td>\n<\/tr>\n
KTA4001<\/td>\nMitochondrial Membrane Potential Assay Kit (JC-1)<\/td>\n<\/tr>\n
A23620<\/td>\nDylight 649, Goat Anti-Rabbit IgG<\/td>\n<\/tr>\n
A01025<\/td>\nAnti-GAPDH Mouse Monoclonal Antibody (2B5), HRP Conjugated<\/td>\n<\/tr>\n
KET9007<\/td>\nEliKine\u2122 Rat TNF-\u03b1 ELISA Kit<\/td>\n<\/tr>\n
ABM0052<\/td>\n\u03b1-SMA Monoclonal Antibody<\/td>\n<\/tr>\n
ABM40111<\/td>\nNFkB p65 Monoclonal Antibody<\/td>\n<\/tr>\n
ABP0163<\/td>\nPI 3-kinase p85\/p55 (phospho Tyr467\/199) Polyclonal Antibody<\/td>\n<\/tr>\n
ABP51894<\/td>\nNa+\/K+-ATPase \u03b11 Polyclonal Antibody<\/td>\n<\/tr>\n
A25012<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG LCS<\/td>\n<\/tr>\n
A25222<\/td>\nIPKine\u2122 HRP, Goat Anti-Rabbit IgG HCS<\/td>\n<\/tr>\n
KTC4300<\/td>\nTraKine\u2122 Pro Live-cell Mitochondrion Staining kit (Deep Red Fluorescence with Super Resolution)<\/td>\n<\/tr>\n
KTD3001<\/td>\nProtein Quantification Kit (BCA Assay)<\/td>\n<\/tr>\n
ABP57259<\/td>\nGAPDH Polyclonal Antibody<\/td>\n<\/tr>\n
BMD00007<\/td>\nBCECF, AM ester<\/td>\n<\/tr>\n
BMD00063<\/td>\nDAPI<\/td>\n<\/tr>\n
BMD00082<\/td>\nAbFluor\u2122 488-Phalloidin<\/td>\n<\/tr>\n
KET7005<\/td>\nEliKine\u2122 Mouse IL-1\u03b2 ELISA Kit<\/td>\n<\/tr>\n
KET6019<\/td>\nEliKine\u2122 Human IL-10 ELISA Kit<\/td>\n<\/tr>\n
KTA3022<\/td>\nCaspase-3 Assay Kit (Colorimetric)<\/td>\n<\/tr>\n
KTB1050<\/td>\nCheKine\u2122 Lipid Peroxidation (MDA) Assay kit<\/td>\n<\/tr>\n
BMS0140<\/td>\nNormal Donkey Serum<\/td>\n<\/tr>\n
K22020<\/td>\nSuperLumia ECL HRP Substrate Kit<\/td>\n<\/tr>\n
K22030<\/td>\nSuperLumia ECL Plus HRP Substrate Kit<\/td>\n<\/tr>\n
KET0001<\/td>\nEliKine\u2122 Testosterone ELISA Kit<\/td>\n<\/tr>\n
KET6013<\/td>\nEliKine\u2122 Human IL-1\u03b2 ELISA Kit<\/td>\n<\/tr>\n
KTC4008<\/td>\nTraKine\u2122 F-actin Staining Kit (Green Fluorescence)<\/td>\n<\/tr>\n
KTC4100<\/td>\nTraKine\u2122 Pro Live-cell Tubulin Staining kit (Green Fluorescence with Super Resolution)<\/td>\n<\/tr>\n
KET7009<\/td>\nEliKine\u2122 Mouse IL-6 ELISA Kit<\/td>\n<\/tr>\n
KTP4003<\/td>\nExKine\u2122 Mitochondrion Extraction Kit (Cultured Cells)<\/td>\n<\/tr>\n
KET0003<\/td>\nEliKine\u2122 Estradiol ELISA Kit<\/td>\n<\/tr>\n
KTC011001<\/td>\nCell Counting Kit-8 (CCK-8)<\/td>\n<\/tr>\n
KET9006<\/td>\nEliKine\u2122 Rat TGF-\u03b21 ELISA Kit<\/td>\n<\/tr>\n
KTA0002<\/td>\nAnnexin V-AbFluor\u2122 488 Apoptosis Detection Kit<\/td>\n<\/tr>\n
KTA1001<\/td>\nLive and Dead Cell Double Staining Kit<\/td>\n<\/tr>\n
KTA3010<\/td>\nCell Senescence Kit (\u03b2-Galactosidase Assay)<\/td>\n<\/tr>\n
K21010<\/td>\nProtein Gel Flash Staining Kit<\/td>\n<\/tr>\n
KTB1400<\/td>\nCheKine\u2122 Nitric Oxide (NO) Assay Kit<\/td>\n<\/tr>\n
KTP3005<\/td>\nExKine\u2122 Membrane and Cytoplasmic Protein Extraction Kit<\/td>\n<\/tr>\n
KTB1100<\/td>\nCheKine\u2122 Lactate Assay Kit<\/td>\n<\/tr>\n
KTB1310<\/td>\nCheKine\u2122 Glucose Oxidase Activity (GOD) Assay Kit<\/td>\n<\/tr>\n
KTC4001<\/td>\nTraKine\u2122 Cell Plasma Membrane Staining Kit (Green Fluorescence)<\/td>\n<\/tr>\n
KET6032<\/td>\nEliKine\u2122 Human TNF-\u03b1 ELISA Kit<\/td>\n<\/tr>\n
A24221<\/td>\nIFKine\u2122 Green Donkey Anti-Rabbit IgG<\/td>\n<\/tr>\n
KTC4210<\/td>\nTraKine\u2122 Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution)<\/td>\n<\/tr>\n
KTA1030<\/td>\nLDH Cytotoxicity Assay Kit<\/td>\n<\/tr>\n
KTD3002<\/td>\nProtein Quantification Kit (Bradford Assay)<\/td>\n<\/tr>\n
KTP21400<\/td>\nPurKine\u2122 Endotoxin Removal Kit (polymyxin B)<\/td>\n<\/tr>\n
KET7015<\/td>\nEliKine\u2122 Mouse TNF-\u03b1 ELISA Kit<\/td>\n<\/tr>\n
ABP50010<\/td>\nCleaved-Caspase-9 p35 (D315) Polyclonal Antibody<\/td>\n<\/tr>\n
KTL0520<\/td>\nLinKine\u2122 AbFluor\u2122488 Labeling Kit<\/td>\n<\/tr>\n
KTA3026<\/td>\nCaspase-9 Assay Kit (Colorimetric)<\/td>\n<\/tr>\n
A24421<\/td>\nIFKine\u2122 Red Donkey Anti-Rabbit IgG<\/td>\n<\/tr>\n
K21010<\/td>\nProtein Gel Flash Staining Kit<\/td>\n<\/tr>\n
A02010AGB<\/td>\nAnti-DDDDK Tag Mouse Monoclonal Antibody (1B10), Agarose<\/td>\n<\/tr>\n
KTL0210<\/td>\nLinKine\u2122 FITC Labeling Kit<\/td>\n<\/tr>\n
KTL0540<\/td>\nLinKine\u2122 AbFluor\u2122 594 Labeling Kit<\/td>\n<\/tr>\n
BMR21504<\/td>\nPurKine\u2122 Anti-DDDDK Tag Resin 4FF<\/td>\n<\/tr>\n
BMD00062<\/td>\nHoechst 33342<\/td>\n<\/tr>\n
BMS0070<\/td>\nNormal Mouse Serum<\/td>\n<\/tr>\n
KTA1030<\/td>\nLDH Cytotoxicity Assay Kit<\/td>\n<\/tr>\n
PRP100274<\/td>\nHuman IL-2 protein<\/td>\n<\/tr>\n
PRP100400<\/td>\nMouse BDNF Protein<\/td>\n<\/tr>\n
ABM0021<\/td>\nGFAP Monoclonal Antibody<\/td>\n<\/tr>\n
ABM0068<\/td>\nPI3 Kinase P85\u03b1 Monoclonal Antibody<\/td>\n<\/tr>\n
KET6033<\/td>\nEliKine\u2122 Human VEGF ELISA Kit<\/td>\n<\/tr>\n
KTE30164<\/td>\nChicken Marek’s disease virus IgG antibody (MDV-Ab-IgG) ELISA Kit<\/td>\n<\/tr>\n
KTA2010<\/td>\nTUNEL Apoptosis Detection Kit (Green Fluorescence)<\/td>\n<\/tr>\n
PRP100159<\/td>\nHuman EGF protein<\/td>\n<\/tr>\n
KET6011<\/td>\nEliKine\u2122 Human IFN-\u03b3 ELISA Kit<\/td>\n<\/tr>\n
KET6027<\/td>\nEliKine\u2122 Human MMP-9 ELISA Kit<\/td>\n<\/tr>\n
KTE62512<\/td>\nHuman Collagen Type I Alpha 1 (COL1A1) ELISA Kit<\/td>\n<\/tr>\n
A01010<\/td>\nAnti-\u03b2-Actin Mouse Monoclonal Antibody (1C7)<\/td>\n<\/tr>\n
A01020<\/td>\nAnti-GAPDH Mouse Monoclonal Antibody (2B5)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

JC-1\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fCAT # KTA4001 (6well\/T) Mitochondri […]<\/p>\n","protected":false},"author":3,"featured_media":29551,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1552],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24740"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24740"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24740\/revisions"}],"predecessor-version":[{"id":49305,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24740\/revisions\/49305"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29551"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24740"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24740"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24740"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}