{"id":24706,"date":"2020-05-13T12:38:14","date_gmt":"2020-05-13T04:38:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24706"},"modified":"2022-07-19T17:15:07","modified_gmt":"2022-07-19T09:15:07","slug":"aposensor-atp-%e7%b4%b0%e8%83%9e%e7%94%9f%e7%89%a9%e7%99%bc%e5%85%89%e6%b8%ac%e5%ae%9a-%e8%b2%a8%e8%99%9fk254","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=24706","title":{"rendered":"ApoSENSOR\u2122 ATP \u7d30\u80de\u751f\u7269\u767c\u5149\u6e2c\u5b9a | \u8ca8\u865fK254"},"content":{"rendered":"<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-614\" style=\"text-align: justify;\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biovision_head.png\" alt=\"bv_head\" width=\"676\" height=\"87\" srcset=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biovision_head.png 676w, http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biovision_head-300x38.png 300w\" sizes=\"(max-width: 676px) 100vw, 676px\" \/><\/p>\n<p style=\"text-align: justify;\"><span style=\"color: #000000;\">\u3000\u3000Biovision\u7814\u767c\u591a\u7a2e\u7522\u54c1\uff0c\u5305\u62ec\u8a66\u5291\u5957\u7d44\u3001\u91cd\u7d44\u86cb\u767d\u3001\u9175\u7d20\u3002\u7522\u54c1\u7dda\u5305\u542b\uff1a\u7d30\u80de\u51cb\u4ea1\u3001\u4ee3\u8b1d\u3001\u7d30\u80de\u589e\u751f\u3001\u6c27\u5316\u58d3\u529b\u3001\u7d30\u80de\u640d\u50b7\u8207\u5fa9\u539f\u3001\u7cd6\u5c3f\u75c5\u3001\u80a5\u80d6\u3001\u4ee3\u8b1d\u75c7\u5019\u7fa4\u3001\u5e79\u7d30\u80de\u751f\u7406\u3001\u57fa\u56e0\u8abf\u7bc0\u3001\u8a0a\u606f\u50b3\u905e\u7b49\u9818\u57df\u4e4b\u7814\u7a76\u5de5\u5177\u3002\u53ef\u7528\u4f86\u5075\u6e2c\u7d30\u80de\u3001\u8840\u6e05\u3001\u53ca\u7d44\u7e54\u7b49\u6a23\u54c1\u7a2e\u985e\u3002<\/span><\/p>\n<p><span style=\"color: #008000;\"><strong>Biovision ADP\/ATP\u5206\u6790\u5957\u7d44<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u5229\u7528luciferin\uff0bATP\u8207lucifarase\u53cd\u61c9\u767c\u51fa\u51b7\u5149\u7684\u7279\u6027\uff0c\u5075\u6e2cATP\u51b7\u5149\u5f37\u5ea6\uff0c\u9032\u4e00\u6b65\u63a8\u7b97ADP\/ATP\u6bd4\u503c\u8207\u7d30\u80de\u5b58\u6d3b\u7684\u95dc\u4fc2\u3002\u9664\u51b7\u5149\u5075\u6e2c\u5916\uff0cBiovision\u5229\u7528\u7279\u5b9a\u7684\u5075\u6e2cprobe\uff0c\u4f7f\u7528\u5316\u5b78\u5448\u8272\u5206\u6790\u7d30\u80de\u4e2dATP\u6216ADP\u542b\u91cf\u3002<\/p>\n<p><span style=\"font-size: medium; color: #008000;\"><strong>ApoSENSOR\u2122 ADP\/ATP \u51b7\u5149\u5206\u6790\u5957\u7d44<\/strong><\/span><\/p>\n<ul>\n<li>\u7d44\u7e54\u3001\u7d30\u80de\u7686\u53ef\u4f7f\u7528\u3002<\/li>\n<li>\u5075\u6e2cATP\u9020\u6210\u51b7\u5149\u500d\u6578\u7684\u6539\u8b8a\uff0c\u9032\u800c\u63a8\u7b97ATP\/ADP\u6bd4\u503c\u3002<\/li>\n<li>\u6709ADP converting Enzyme, \u53ef\u5c07ADP\u8f49\u8b8a\u70baATP\uff0c\u9054\u5230\u6e2c\u91cfADP\/ATP\u7684\u76ee\u7684\u3002<\/li>\n<li>\u51b7\u5149\u5075\u6e2c\u9ad8\u654f\u611f\u5ea6\uff08100 cells\/well\uff09\uff0c\u9069\u7528\u65bc\u5927\u91cf\u5206\u6790\u4f7f\u7528\u3002<\/li>\n<\/ul>\n<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=24706\">ApoSENSOR\u2122 ATP \u7d30\u80de\u751f\u7269\u767c\u5149\u6e2c\u5b9a | <strong>\u8ca8\u865f<\/strong>K254<\/a><\/span><\/p>\n<p>ApoSENSOR\u2122 ATP Cell Viability Bioluminescence Assay Kit<\/p>\n<p>\u7522\u54c1\u4ecb\u7d39:\u7d30\u80de\u6b7b\u4ea1(Cell death ) \uff0c\u7279\u5225\u662f\u51cb\u4ea1(apoptosis)\u662f\u4e00\u500b\u80fd\u91cf\u4f9d\u8cf4\u7684\u904e\u7a0b,\u9700\u8981ATP\u3002\u7576ATP\u6c34\u6e96\u4e0b\u964d\u5230\u7d30\u80de\u4e0d\u518d\u80fd\u5920\u57f7\u884c\u57fa\u672c\u4ee3\u8b1d\u529f\u80fd\u7684\u7a0b\u5ea6\u6642,\u7d30\u80de\u5c07\u6b7b\u4ea1\u3002\u5178\u578b\u7684\u51cb\u4ea1\u7d30\u80de\u986f\u793aATP\u6c34\u5e73\u986f\u8457\u4e0b\u964d\u3002\u56e0\u6b64,\u7d30\u80de\u4e2dATP\u6c34\u5e73\u7684\u55aa\u5931\u88ab\u7528\u4f5c\u7d30\u80de\u6b7b\u4ea1(cell death)\u7684\u6307\u6a19(indicator)\u3002\u76f8\u6bd4\u4e4b\u4e0b,\u7d30\u80de\u589e\u6b96(cell proliferation)\u5df2\u88abATP\u6c34\u6e96\u5347\u9ad8\u6240\u8b58\u5225\u3002ApoSENSOR\u2122\u7d30\u80de\u53ef\u751f\u5b58\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2(ApoSENSOR\u2122 Cell Viability Assay Kit, K254) \u5229\u7528ATP\u6c34\u6e96\u7684\u751f\u7269\u767c\u5149\u6aa2\u6e2c,\u540c\u6642\u5feb\u901f\u7be9\u9078\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u4e2d\u7684\u51cb\u4ea1(apoptosis)\u548c\u7d30\u80de\u589e\u6b96(cell proliferation)\u3002\u8a72\u6e2c\u5b9a\u5229\u7528\u87a2\u5149\u9176\u50ac\u5316\u4f86\u81eaATP\u548c\u87a2\u5149\u7d20\u7684\u5149\u7684\u5f62\u6210,\u4e26\u4e14\u53ef\u4ee5\u4f7f\u7528luminometer\u6216Beta\u8a08\u6578\u5668\u6e2c\u91cf\u5149\u3002\u8a72\u6e2c\u5b9a\u53ef\u4ee5\u5b8c\u5168\u81ea\u52d5\u9032\u884c\u9ad8\u901a\u91cf(10\u79d2\/\u6a23\u54c1),\u4e26\u4e14\u6975\u5176\u654f\u611f(\u6aa2\u6e2c10-100\u500b\u54fa\u4e73\u52d5\u7269\u7d30\u80de\/\u4e95)\u3002\u9019\u7a2e\u6e2c\u5b9a\u7684\u9ad8\u9748\u654f\u5ea6\u5c0e\u81f4\u8a31\u591a\u5176\u4ed6\u61c9\u7528\u6aa2\u6e2cATP\u751f\u7522\u5728\u5404\u7a2e\u9176\u53cd\u61c9,\u4ee5\u53ca\u6aa2\u6e2c\u4f4e\u6c34\u6e96\u7d30\u83cc\u6c61\u67d3\u6a23\u54c1,\u5982\u8840\u6db2,\u725b\u5976,\u5c3f\u6db2,\u571f\u58e4\u548c\u6c61\u6ce5\u3002<\/p>\n<p><img decoding=\"async\" class=\"cboxPhoto\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/2\/k254.jpg\" alt=\"\" \/><\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Luminometer or Beta Counter.<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Mammalian<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells.<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Simple one-step procedure; takes only 30 minutes \u2022 Fast and convenient \u2022 The assay can be done directly in culture plates requiring no harvest\/washing\/or sample preparations. The assay can be fully automatic for high throughput (10 seconds\/sample) and is highly sensitive (detects 10-100 mammalian cells\/well).<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 Nucleotide Releasing Buffer \u2022 ATP Monitoring Enzyme \u2022 Enzyme Reconstitution Buffer \u2022 ATP (MW 551)<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage Conditions<\/strong><\/td>\n<td>-20\u00b0C<\/td>\n<\/tr>\n<tr>\n<td><strong>Shipping Conditions<\/strong><\/td>\n<td>Gel Pack<\/td>\n<\/tr>\n<tr>\n<td><strong>USAGE<\/strong><\/td>\n<td>For Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?p=7010\">\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0<a href=\"http:\/\/biopioneer.com.tw\/?p=18043\">FRAP\u6aa2\u6e2c<\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?p=8004\"><strong>\u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/strong><\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?p=18876\">Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?p=6035\">\u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?p=9470\">Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?p=14101\"><strong>\u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong><strong>MDA<\/strong><strong>\uff09\u6e2c\u5b9a<\/strong><\/a><strong> | <\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=15520\"><strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a><strong> BCAA<\/strong><strong>\u6aa2\u6e2c<\/strong><\/p>\n<p>&nbsp;<\/p>\n<table style=\"width: 582px;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td nowrap=\"nowrap\" width=\"100\">\n<p align=\"center\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/p>\n<\/td>\n<td nowrap=\"nowrap\" width=\"476\">\n<p align=\"center\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/p>\n<\/td>\n<td nowrap=\"nowrap\" width=\"100\">\n<p align=\"center\"><strong>\u5305\u88dd<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" nowrap=\"nowrap\" width=\"582\">\n<p align=\"center\"><span style=\"color: #333399;\"><strong>\u51b7\u5149\u5448\u8272\u5206\u6790<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" nowrap=\"nowrap\" width=\"84\">\u00a0K255-200<\/td>\n<td nowrap=\"nowrap\" width=\"409\">\u00a0ApoSENSOR\u2122 ADP\/ATP Ratio Bioluminescence Assay Kit<\/td>\n<td nowrap=\"nowrap\" width=\"88\">\n<p align=\"center\">200 assays<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" nowrap=\"nowrap\" width=\"84\">\u00a0K254-200<br \/>\n<span style=\"font-size: 11px; line-height: 1.5em;\">K254-1000<\/span><\/td>\n<td nowrap=\"nowrap\" width=\"409\">\u00a0ApoSENSOR\u2122 ATP Cell Viability Bioluminescence Assay Kit<\/td>\n<td nowrap=\"nowrap\" width=\"88\">\n<p align=\"center\">200 assays<br \/>\n1000 assays<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" nowrap=\"nowrap\" width=\"84\">\u00a0K791-100<br \/>\n<span style=\"font-size: 11px; line-height: 1.5em;\">K791-1000<\/span><\/td>\n<td nowrap=\"nowrap\" width=\"409\">\u00a0StayBrite\u2122 Highly Stable ATP Bioluminescence Assay Kit\u2014\u9577\u6548\u578b\u51b7\u5149ATP\u5206\u6790<\/td>\n<td nowrap=\"nowrap\" width=\"88\">\n<p align=\"center\">100 assays<br \/>\n1000 assays<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" nowrap=\"nowrap\" width=\"582\">\n<p align=\"center\"><span style=\"color: #333399;\"><strong>\u5316\u5b78\u5448\u8272\u5206\u6790<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" nowrap=\"nowrap\" width=\"84\">\u00a0K356-100<\/td>\n<td nowrap=\"nowrap\" width=\"409\">\u00a0ADP Colorimetric Assay Kit II<\/td>\n<td nowrap=\"nowrap\" width=\"88\">\n<p align=\"center\">100 assays<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" nowrap=\"nowrap\" width=\"84\">\u00a0K355-100<\/td>\n<td nowrap=\"nowrap\" width=\"409\">\u00a0ADP Colorimetric\/Fluorometric Assay Kit<\/td>\n<td nowrap=\"nowrap\" width=\"88\">\n<p align=\"center\">100 assays<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" nowrap=\"nowrap\" width=\"84\">\u00a0K354-100<\/td>\n<td nowrap=\"nowrap\" width=\"409\">\u00a0ATP Colorimetric\/Fluorometric Assay Kit<\/td>\n<td nowrap=\"nowrap\" width=\"88\">\n<p align=\"center\">100 assays<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u3000Biovision\u7814\u767c\u591a\u7a2e\u7522\u54c1\uff0c\u5305\u62ec\u8a66\u5291\u5957\u7d44\u3001\u91cd\u7d44\u86cb\u767d\u3001\u9175\u7d20\u3002\u7522\u54c1\u7dda\u5305\u542b\uff1a\u7d30\u80de\u51cb\u4ea1\u3001\u4ee3\u8b1d\u3001\u7d30\u80de\u589e\u751f\u3001\u6c27\u5316\u58d3 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1562],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24706"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24706"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24706\/revisions"}],"predecessor-version":[{"id":24707,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24706\/revisions\/24707"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24706"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24706"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24706"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}