{"id":24602,"date":"2020-05-09T14:04:24","date_gmt":"2020-05-09T06:04:24","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24602"},"modified":"2022-07-19T17:18:32","modified_gmt":"2022-07-19T09:18:32","slug":"anti-pdl1-%e6%8a%97%e9%ab%94-absoluteantibody-%e8%b2%a8%e8%99%9f-ab00373-1-32","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=24602","title":{"rendered":"Anti-PDL1 \u6297\u9ad4 |absoluteantibody \u8ca8\u865f Ab00373-1.32"},"content":{"rendered":"

Anti-PDL1 [YDC 127.1.1], Mouse IgG1-D265A, Fc Silenced, Kappa\u6297\u9ad4 | Anti-TIGIT [1B4], Mouse IgG1, Lambda\u8ca8\u865f\u00a0Ab00373-1.32<\/a> <\/strong>\u00a0\u00a0<\/strong><\/p>\n

\u6297\u9ad4\u4ecb\u7d39<\/strong>:<\/strong>\u00a0\u00a0Anti-PDL1 \u6297\u9ad4\u5c0dmurine PDL1.\u7684\u7d50\u5408\u7279\u7570\u6027 (Specificity)\u3002\u6b64\u6297\u9ad4\u53ef\u61c9\u7528\u65bcELISA, FC, IF\u7b49\u5be6\u9a57\u3002<\/p>\n

\"Binding<\/p>\n

Flow-cytometry using anti-PD-L1 antibody YDC 127.1.1 (Ab00373)\u00a0<\/b>Mouse spleenocytes
\nwere stained with an isotype control (Ab00102-23.0, purple) or the rabbit-chimeric version of YDC 127.1.1 (Ab00373-23.0, grey) at a concentration of 1 \u00b5g\/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.<\/p>\n

\"Binding<\/p>\n

Immunofluorescence staining of fixed mouse splenocytes with anti-PDL1 (CD274) antibody YDC 127.1.1 (Ab00373)\u00a0<\/b>Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus<\/i>) splenocytes on Shi-fix\u2122 coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of YDC 127.1.1 (Ab00373-23.0) at 10 \u00b5g\/ml for 1h followed by Alexa Fluor\u00ae\u00a0488 secondary antibody (1 \u00b5g\/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00373-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor\u00ae\u00a0488 secondary antibody.<\/p>\n

\"Binding<\/p>\n

Flow-cytometry using anti-PDL1 antibody YDC 127.1.1 (Ab00373)\u00a0<\/b>Mouse lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of YDC 127.1.1 (Ab00373-23.0, panel B) at a concentration of 1 \u00b5g\/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.<\/p>\n

Alternative Name(s) of Target: : CD274; Programmed death-ligand 1; PD-L1; B7 Homolog 1; B7-H1; B7-H; PDCD1L1; PDCD1LG1<\/p>\n

\u514d\u75ab\u539f(Immunogen: Murine PDL1 UniProt Accession Number of Target Protein: Q9EP73<\/p>\n

Size: 100 \u03bcg Purified antibody. Purification: Protein A affinity purified Supplied In: PBS with 0.02% Proclin 300. Storage Recommendation: Store at 4\u2070C for up to 3 months. For longer storage, aliquot and store at – 20\u2070C. Concentration: 1 mg\/ml.<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\nMouse target<\/td>\n\u6297\u9ad4clone<\/td>\nOriginal Antibody format<\/td>\n<\/tr>\n
Ab01052<\/a><\/td>\n4-1BB (CD137)<\/td>\nLOB12.3<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n<\/thead>\n
Ab01054<\/a><\/td>\n4-1BBL (CD137L)<\/td>\nAT113-2<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00881<\/a><\/td>\n4-1BBL (CD137L)<\/td>\nTKS-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01027<\/a><\/td>\nBTLA<\/td>\nHMBT-6B2
\n<\/a><\/td>\n		Hamster IgG<\/td>\n<\/tr>\n
Ab00670<\/a><\/td>\nCD27<\/td>\nLG.3A10<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00895<\/a><\/td>\nCD27<\/td>\n\n

RM27-3E5<\/a><\/p>\n

 <\/td>\n

Rat IgG2a<\/td>\n<\/tr>\n
Ab00286<\/a><\/td>\nCD28<\/td>\nE18
\n<\/a><\/td>\n		Mouse IgG2b<\/td>\n<\/tr>\n
Ab00285<\/a><\/td>\nCD28<\/td>\nD665
\n<\/a><\/td>\n		Mouse IgG1<\/td>\n<\/tr>\n
Ab01109<\/a><\/td>\nCD40<\/td>\n3\/23
\n<\/a><\/td>\n		Rat IgG2a<\/td>\n<\/tr>\n
Ab01087<\/a><\/td>\nCD40L (CD154)<\/td>\nMR1
\n<\/a><\/td>\n		Hamster IgG<\/td>\n<\/tr>\n
Ab00816<\/a><\/td>\nCD70<\/td>\nTAN 1-7
\n<\/a><\/td>\n		Rat IgG2a<\/td>\n<\/tr>\n
Ab01056<\/a><\/td>\nCD70<\/td>\nTAN1-6
\n<\/a><\/td>\n		Rat IgG2a<\/td>\n<\/tr>\n
Ab01028<\/td>\nCD80 (B7-1)<\/td>\nRM80<\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01029<\/a><\/td>\nCD86 (B7-2)<\/td>\nPO.3<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab01089<\/a><\/td>\nCD153<\/td>\nRM153<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab01297<\/a><\/td>\nCD96<\/td>\n6A6<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00894<\/a><\/td>\nCTLA-4<\/td>\n9H10<\/a><\/td>\nHamster IgG2<\/td>\n<\/tr>\n
Ab01018<\/a><\/td>\nCTLA-4<\/td>\n9D9<\/a><\/td>\nMouse IgG2b<\/td>\n<\/tr>\n
Ab01088<\/a><\/td>\nGAL-9<\/td>\nRG9-35<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01060<\/a><\/td>\nGITR<\/td>\nDTA-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00374<\/a><\/td>\nGITR<\/td>\nYGITR 860.103.5<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00631<\/a><\/td>\nGITR<\/td>\nYGITR765<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00630<\/a><\/td>\nGITRL<\/td>\nYGL386<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01026<\/a><\/td>\nHVEM<\/td>\nHMHV-1B18<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00814<\/a><\/td>\nICOS<\/td>\n7E.17G9<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00882<\/a><\/td>\nICOSL<\/td>\nHK5.3<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01295<\/a><\/td>\nLAG3<\/td>\nC9B7W<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01295<\/a><\/td>\nLAG3<\/td>\nC9B7W<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00110<\/a><\/td>\nOX40 (CD134)<\/td>\nOX86<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00564<\/a><\/td>\nOX40L<\/td>\nOX-89<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00813<\/a><\/td>\nPD-1<\/td>\nRMP1-14<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01017<\/a><\/td>\nPD-1H (VISTA)<\/td>\nmam82<\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01016<\/a><\/td>\nPD-1H (VISTA)<\/td>\nMH5A<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00373<\/a><\/td>\nPD-L1<\/td>\nYDC 127.1.1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00883<\/a><\/td>\nPD-L2<\/td>\nTY25<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00875<\/a><\/td>\nPDPN (MAP-Tag)<\/td>\nPMab-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00973<\/a><\/td>\nTIGIT<\/td>\n4D4<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab01258<\/a><\/td>\nTIGIT<\/td>\n1B4<\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01032<\/a><\/td>\nTIM-1<\/td>\n3B3<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01110<\/a><\/td>\nTIM-2<\/td>\nRMT2-14<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01057<\/a><\/td>\nTIM-3<\/td>\n2C12<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01058<\/a><\/td>\nTIM-4<\/td>\n5G3<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01059<\/a><\/td>\nTIM-4<\/td>\n3A1<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Anti-PDL1 [YDC 127.1.1], Mouse IgG1-D265A, Fc Silenced, […]<\/p>\n","protected":false},"author":3,"featured_media":3519,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,3],"tags":[1569],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24602"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24602"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24602\/revisions"}],"predecessor-version":[{"id":24604,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24602\/revisions\/24604"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/3519"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24602"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24602"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}