{"id":24135,"date":"2020-03-22T12:37:38","date_gmt":"2020-03-22T04:37:38","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24135"},"modified":"2022-11-30T17:03:10","modified_gmt":"2022-11-30T09:03:10","slug":"%e7%84%a1%e6%a9%9f%e5%a4%9a%e7%a3%b7%e9%85%b8%e9%b9%bd%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk2025","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=24135","title":{"rendered":"\u7121\u6a5f\u591a\u78f7\u9178\u9e7d\u6aa2\u6e2c | BioVision\u2019s Inorganic Polyphosphate Assay Kit\u8ca8\u865fK2025 (ABCAM ab284528)"},"content":{"rendered":"

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\u7121\u6a5f\u591a\u78f7\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K2025<\/a>\u00a0\u00a0(ABCAM ab284528)<\/span><\/p>\n

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\u7522\u54c1\u63cf\u8ff0:<\/strong> \u7121\u6a5f\u591a\u78f7\u9178\u9e7d\uff08Inorganic polyphosphate , Poly P\uff09\u662f\u7531\u6578\u767e\u500b\u6b63\u78f7\u9178\u9e7d(Inorganic polyphosphate)\uff08Pi\uff09\u6b98\u57fa\u901a\u904e\u9ad8\u80fd\u78f7\u9178\u9150\u9375(phosphoanhydride bonds.)\u9023\u63a5\u7684\u7dda\u6027\u805a\u5408\u7269(linear polymer)\u3002\u805a\u78f7 (Poly P)\u7121\u8655\u4e0d\u5728\uff0c\u53ef\u4ee5\u5b58\u5728\u65bc\u5f9e\u7d30\u83cc\u5230\u54fa\u4e73\u52d5\u7269\u7684\u6240\u6709\u6d3b\u751f\u7269\u9ad4\u4e2d\u3002\u805a\u78f7 (Poly P)\u5177\u6709\u8a31\u591a\u91cd\u8981\u7684\u7d30\u80de\u529f\u80fd\u3002\u5728\u5fae\u751f\u7269\u4e2d\uff0c\u805a\u78f7 (Poly P)\u5728\u6bd2\u529b(virulence)\uff0c\u751f\u7269\u819c\u5f62\u6210(biofilm formation)\uff0c\u904b\u52d5(motility)\uff0c\u7fa4\u9ad4\u611f\u61c9(quorum sensing)\uff0c\u61c9\u6fc0\u53cd\u61c9(stress response)\u548c\u5728\u71df\u990a\u7f3a\u4e4f\u671f\u9593\u5b58\u6d3b\u4e2d\u5177\u91cd\u8981\u4f5c\u7528\u3002\u5728\u54fa\u4e73\u52d5\u7269\u4e2d\uff0c\u5b83\u5c0d\u65bc\u51dd\u8840(blood coagulation)\uff0c\u9223\u6c89\u6fb1(calcium precipitation)\uff0c\u514d\u75ab\u53cd\u61c9(immune response)\uff0c\u7d30\u80de\u51cb\u4ea1(apoptosis)\uff0c\u4fe1\u865f\u8f49\u5c0e(signal transduction)\u548c\u7c92\u817a\u9ad4\u4ee3\u8b1d(mitochondrial metabolism)\u626e\u6f14\u91cd\u8981\u89d2\u8272\u3002\u805a\u78f7 (Poly P)\u5728\u764c\u7d30\u80de\u589e\u6b96(cancer cell proliferation)\u4e2d\u4e5f\u5177\u91cd\u8981\u4f5c\u7528\u3002\u805a\u78f7 (Poly P)\u5b9a\u91cf\u7684\u50b3\u7d71\u6e2c\u5b9a\u65b9\u6cd5\uff0c\u70ba\u4f7f\u7528\u653e\u5c04\u6027\u540c\u4f4d\u7d20\u65b9\u6cd5\uff0c\u8a72\u65b9\u6cd5\u4e0d\u65b9\u4fbf\u57f7\u884c\u3002 BioVision\u7121\u6a5f\u591a\u78f7\u9178\u9e7d\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2(BioVision\u2019s Inorganic Polyphosphate Assay Kit, K2025) \u662f\u4e00\u7a2e\u7c21\u55ae\u7684\u6e2c\u5b9a\u65b9\u6cd5\uff0c\u53ef\u4ee5\u5b9a\u91cf\u5404\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u7684\u805a\u78f7 (Poly P)\u542b\u91cf\u3002\u8a72\u6e2c\u5b9a\u4f7f\u7528\u4e00\u7a2e\u87a2\u5149\u67d3\u6599\uff0c\u8a72\u87a2\u5149\u67d3\u6599\u8207\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u805a\u78f7 (Poly P)\u5f62\u6210\u8907\u5408\u7269\uff0c\u4e26\u5728Ex \/ Em = 415\/550 nm\u4e0b\u6e2c\u91cf\u8a72\u87a2\u5149\u8907\u5408\u7269\u3002\u87a2\u5149\u4fe1\u865f\u8207\u6a23\u54c1\u4e2d\u7684Poly P\u6fc3\u5ea6\u6210\u6b63\u6bd4\u3002 BioVision\u7684\u7121\u6a5f\u805a\u78f7\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2(BioVision\u2019s Inorganic Polyphosphate Assay Kit, K2025)\u662f\u4e00\u7a2e\u5feb\u901f\uff0c\u9748\u654f\u4e14\u65b9\u4fbf\u7684\u6aa2\u6e2cPoly P\u7684\u5de5\u5177\u3002\u5728\u6aa2\u6e2c\u689d\u4ef6\u4e0b\uff0c\u5b83\u53ef\u4ee5\u6aa2\u6e2c\u4f4e\u81f350 pmole\u3002<\/p>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0\u00a0\u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/strong>\u6aa2\u6e2c<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n100 assay<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em = 415\/550 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetermination of Poly P in different biological samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 A convenient tool for detecting Inorganic polyphosphate in various biological samples
\n\u2022 Sensitive
\n\u2022 Rapid<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Poly P Assay Buffer
\n\u2022 Poly P Extraction Buffer
\n\u2022 Poly P Standard (45 mer)
\n\u2022 Poly P Dye
\n\u2022 RNase
\n\u2022 DNase
\n\u2022 Proteinase K<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

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