{"id":24128,"date":"2020-03-22T11:44:36","date_gmt":"2020-03-22T03:44:36","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24128"},"modified":"2022-07-19T17:24:12","modified_gmt":"2022-07-19T09:24:12","slug":"cd274%e6%85%a2%e7%97%85%e6%af%92%e8%a1%a8%e7%8f%be%e8%bc%89%e9%ab%94-cd274-lentiviral-vectorplenti-giii-cmv-%e8%b2%a8%e8%99%9f155160610195","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=24128","title":{"rendered":"CD274\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4 | CD274 Lentiviral Vector(pLenti-GIII-CMV) \u8ca8\u865f155160610195"},"content":{"rendered":"

CD274 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) <\/strong>\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4 | \u8ca8\u865f155160610195<\/a><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:<\/strong> CD274 Lentiviral Vector (Human) (CMV) (<\/u>BC113734) <\/u>\u67b6\u69cb\u65bcpLenti-GIII-CMV<\/a>\u8868\u73fe\u8f09\u9ad4\u4e0a\uff0c\u69cb\u6210CD274 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)\u3002\u6162\u75c5\u6bd2\u8f09\u9ad4\uff0c\u53ef\u7d50\u5408packaging mix (\u6539\u826f\u5f0f\u00a0<\/strong>\u75c5\u6bd2\u5305\u88dd\u7cfb\u7d71\u00a0<\/strong><\/a><\/span>LV003)<\/span> <\/strong><\/span>\u4ee5\u5728\u591a\u7a2e\u5bbf\u4e3b\u7d30\u80de\u6216\u52d5\u7269\u6a21\u578b\u4e2d\u904e\u8868\u9054\u76ee\u6a19miRNA\u3002 \u8a72\u69cb\u5efa\u9ad4\u542b\u6709\u7528\u65bc\u76e3\u6e2c\u8f49\u67d3\u6548\u7387\u7684GFP\u5831\u544a\u57fa\u56e0(GFP reporter )\uff0c\u53ef\u4ee5Monitor\u8f49\u67d3\u6548\u7387\u3002\u53ef\u76f4\u63a5\u4ee5\u975e\u75c5\u6bd2\u8cea\u7c92\u8f49\u67d3 (non-viral plasmid transfection) \u7acb\u5373\u8868\u9054\u76ee\u6a19\u86cb\u767d\uff0c\u6216\u7d93\u7531\u5305\u88dd\u6210\u6162\u75c5\u6bd2\u9846\u7c92\uff0c\u5be6\u73fe\u9ad8\u6548\u8f49\u5c0e\u548c\u7a69\u5b9a\u6574\u5408\u7684\u8868\u9054\u76ee\u6a19\u86cb\u767d\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/td>\nCD274 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)<\/td>\n<\/tr>\n
Accession Number<\/td>\nBC113734<\/td>\n<\/tr>\n
Description<\/td>\nThe CD274 expression will be driven by a CMV promoter, without any Tag or reporter. Inserts are flanked by and can be excised using NheI and XhoI as long as inserts do not contain any internal NheI or XhoI sites.<\/td>\n<\/tr>\n
Cat.No<\/td>\n155160610195<\/td>\n<\/tr>\n
Gene Name<\/td>\nCD274<\/td>\n<\/tr>\n
Full Gene Name<\/td>\nCD274 molecule<\/td>\n<\/tr>\n
Aliases<\/td>\nB7-H, B7H1, PD-L1, PDL1<\/td>\n<\/tr>\n
List of species<\/td>\nHuman<\/td>\n<\/tr>\n
Units<\/td>\n1.0 \u00b5g DNA<\/td>\n<\/tr>\n
Vector<\/td>\npLenti-GIII-CMV<\/a><\/td>\n<\/tr>\n
Applications<\/td>\n\n
    \n
  • Direct non-viral plasmid transfection for immediate expression<\/li>\n
  • Package into lentiviral particles for high efficiency transduction and stably integrated expression<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
System<\/td>\nLentiviral Vector<\/td>\n<\/tr>\n
Insert Size<\/td>\n873bp<\/td>\n<\/tr>\n
List of Markers<\/td>\nBacterial: Kanamycin. Mammalian: Puromycin<\/td>\n<\/tr>\n
Promoters<\/td>\nCMV<\/td>\n<\/tr>\n
Appearance<\/td>\nClear Liquid<\/td>\n<\/tr>\n
Vector Size<\/td>\n8074bp<\/td>\n<\/tr>\n
Format<\/td>\nLentiviral Plasmid<\/td>\n<\/tr>\n
Storage Buffer<\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Storage<\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n
Internal Leadtime<\/td>\nIn Stock*
\n*lead time will be 1-2 weeks for a Quality Control check prior to shipment<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

CD274 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) […]<\/p>\n","protected":false},"author":3,"featured_media":24012,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1611],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24128"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24128"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24128\/revisions"}],"predecessor-version":[{"id":24129,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24128\/revisions\/24129"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24012"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24128"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24128"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24128"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}