{"id":24122,"date":"2020-03-22T10:55:29","date_gmt":"2020-03-22T02:55:29","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24122"},"modified":"2022-07-19T17:24:26","modified_gmt":"2022-07-19T09:24:26","slug":"ace2-orf-vector-abmgood%e8%b2%a8%e8%99%9f1116701","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=24122","title":{"rendered":"ACE2 ORF Vector| ABMGOOD\u8ca8\u865f1116701"},"content":{"rendered":"

ACE2 ORF Vector| <\/strong>\u8ca8\u865f<\/strong>1116701 (1.0 \u00b5g DNA)<\/a><\/span><\/p>\n

Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u5e8f\u5217\u9a57\u8b49\u958b\u653e\u95b1\u8b80\u6846\uff08ORF\uff09\u5eab\uff0c\u5305\u62ec\u4eba(human)\uff0c\u5c0f\u9f20(mouse)\u548c\u5927\u9f20(rat)\uff0c\u53efinsertion\u81f3\u60a8\u9078\u64c7\u7684\u86cb\u767d\u8cea\u8868\u9054\u7cfb\u7d71(protein expression systems.)\u3002\u53bb\u96645’UTR\u548c3’UTR\uff0cORF\u63d2\u5165\u517c\u5bb9\u7684\u8868\u9054\u8f09\u9ad4(compatible expression vector) \u5f8c\u5373\u53ef\u5feb\u901f\u53ef\u9760\u5730\u8868\u9054\u86cb\u767d\u8cea\u3002\u4e5f\u53ef\u7528\u4f5cPCR\u64f4\u589e (PCR amplification experiments) \u5be6\u9a57\u4e2d\u967d\u6027\u5c0d\u7167(positive control)\u7684\u6a21\u677f(template)\u3002Abmgood\u63d0\u4f9b\u7684ORF\u5305\u542b\u4e00\u500b\u7a81\u8b8a\u7684\u7d42\u6b62\u5bc6\u78bc\u5b50(mutated stop codon)\uff0c\u5982\u679c\u53d7\u9ad4\u8f09\u9ad4\u4e2d\u5b58\u5728C\u672b\u7aef\u6a19\u7c64\u6216\u5831\u544a\u57fa\u56e0\uff0c\u5247\u5141\u8a31\u5176\u647b\u5165\u3002 abm\u7684ORF\u4e5f\u53ef\u4ee5\u4ee5\u5373\u7528\u578b\u86cb\u767d\u8868\u9054\u8f09\u9ad4(protein expression vectors<\/a>)\uff0c\u6162\u75c5\u6bd2\u8f09\u9ad4(lentiviral vectors<\/a>)\u6216\u5305\u88dd\u7684\u6162\u75c5\u6bd2(\u00a0lentivirus<\/a>)\u548c\u817a\u75c5\u6bd2(\u00a0adenovirus\u00a0<\/a>)\u4e4b\u67b6\u69cb\u597d\u4e4b\u8f09\u9ad4\u6216\u75c5\u6bd2\u6db2\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n

The ACE2 ORF Vector holds the gene (cloned by a restriction enzyme-independent method) between an AflII and EcoRV cut site. Inserts are flanked by and can be excised using AflII and EcoRV as long as inserts do not contain any internal AflII and EcoRV sites. In the case that the insert contains these internal cut sites, the end user can PCR amplify the insert with preferred restriction sites for subcloning.<\/p>\n

ACE2 ORF<\/strong>\u8f09\u9ad4\u4ecb\u7d39: ACE2 ORF\u8f09\u9ad4\uff1b Accession Number <\/strong>BC039902<\/a>,NM_027286<\/a>,NM_001130513<\/a>,NM_001012006<\/a>\u3002\u5728AflII\u548cEcoRV\u5207\u5272\u4f4d\u9ede\u4e4b\u9593\u4fdd\u7559\u4e86\u8a72\u57fa\u56e0\uff08\u9650\u5236\u6027\u9176\u5207\u65b9\u6cd5\uff09\u3002Inserts\u4e0d\u5305\u542b\u4efb\u4f55\u5167\u90e8AflII\u548cEcoRV cut site\uff0c\u53ef\u4ee5AflII\u548cEcoRV\u5207\u9664\u3002\u5728\u63d2\u5165\u7247\u6bb5\u5305\u542b\u9019\u4e9b\u5167\u90e8\u5207\u5272\u4f4d\u9ede\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5PCR\u64f4\u589e\u5e36\u6709\u512a\u9078\u9650\u5236\u6027\u9176\u5207\u4f4d\u9ede\u7684\u63d2\u5165\u7247\u6bb5\uff0c\u4ee5\u9032\u884csubcloning\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Description<\/em><\/td>\nThe ACE2 ORF Vector holds the gene (cloned by a restriction enzyme-independent method) between an AflII and EcoRV cut site. Inserts are flanked by and can be excised using AflII and EcoRV as long as inserts do not contain any internal AflII and EcoRV sites. In the case that the insert contains these internal cut sites, the end user can PCR amplify the insert with preferred restriction sites for subcloning.<\/td>\n<\/tr>\n
HGNC ID<\/em><\/td>\nHGNC:13557<\/td>\n<\/tr>\n
Accession Number<\/em><\/td>\nBC039902,NM_027286,NM_001130513,NM_001012006<\/td>\n<\/tr>\n
Accession Number<\/em><\/td>\nBC039902<\/a>,NM_027286<\/a>,NM_001130513<\/a>,NM_001012006<\/a><\/td>\n<\/tr>\n
Gene Name<\/em><\/td>\nACE2<\/td>\n<\/tr>\n
Full Gene Name<\/em><\/td>\nangiotensin I converting enzyme (peptidyl-dipeptidase A) 2<\/td>\n<\/tr>\n
Appearance<\/em><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Insert Size<\/em><\/td>\nBC039902:2418
\nNM_027286:2418
\nNM_001130513:2418
\nNM_001012006:2418<\/td>\n<\/tr>\n
Vector Size<\/em><\/td>\n2596bp<\/td>\n<\/tr>\n
Storage Buffer<\/em><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Species<\/em><\/td>\nThis gene is available from: Human,Mouse,Rat<\/td>\n<\/tr>\n
List of bacterial markers<\/em><\/td>\nSpectinomycin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

ACE2 ORF Vector| \u8ca8\u865f1116701 (1.0 \u00b5g DNA) Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u5e8f\u5217\u9a57 […]<\/p>\n","protected":false},"author":3,"featured_media":24012,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1613],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24122"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24122"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24122\/revisions"}],"predecessor-version":[{"id":24127,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24122\/revisions\/24127"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24012"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24122"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24122"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24122"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}