{"id":24044,"date":"2020-03-14T17:30:17","date_gmt":"2020-03-14T09:30:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24044"},"modified":"2024-01-26T18:33:54","modified_gmt":"2024-01-26T10:33:54","slug":"%e8%8d%89%e9%85%b0%e4%b9%99%e9%85%b8%e6%af%94%e8%89%b2%e6%b3%95%e8%9e%a2%e5%85%89%e6%b8%ac%e5%ae%9a%e2%80%8b%e2%80%8b%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk659-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=24044","title":{"rendered":"\u8349\u9170\u4e59\u9178\u6bd4\u8272\u6cd5\/\u87a2\u5149\u6e2c\u5b9a\u200b\u200b\u8a66\u5291\u76d2 | \u8ca8\u865fK659-100 (abcam ab83428)"},"content":{"rendered":"

\u00a0<\/strong>Home<\/strong><\/a> \u00a0 \u00a0<\/strong>Literature<\/strong><\/a>\u00a0 \u00a0<\/strong>Promotions<\/strong><\/a>\u00a0\u00a0<\/strong>Product Citations<\/strong><\/a><\/p>\n

\u8349\u9170\u4e59\u9178\u6bd4\u8272\u6cd5\/\u87a2\u5149\u6e2c\u5b9a\u200b\u200b\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K659-100 (abcam ab83428)<\/a><\/span><\/p>\n

Oxaloacetate Colorimetric\/Fluorometric Assay Kit<\/em><\/strong>\u00a0<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u8349\u9170\u4e59\u9178\u916f\uff08Oxaloacetate , OAA\uff0cHOOC-CO-CH 2 -COOH\uff09\u662fTCA\u5faa\u74b0\u4e2d\u9593\u9ad4(TCA cycle intermediate)\u3002\u5b83\u5148\u65bc\u7531\u4e59\u9170\u57fa(acetyl group)\u8f49\u79fb\u81f3OAA\u5f62\u6210\u7684\u6ab8\u6aac\u9178\u9e7d(citrate)\u3002 OAA\u662f\u7531\u5929\u51ac\u6c28\u9178\u812b\u9170\u80fa(aspartate)\u6216\u4e8c\u6c27\u5316\u78b3\u8207\u4e19\u916e\u9178\u6216PEP\u7e2e\u5408\u5f62\u6210\u7684\u3002\u7531\u65bc\u54fa\u4e73\u52d5\u7269\u4e0d\u5177\u5099\u5f9e\u4e59\u9170\u8f14\u9176A\u5f62\u6210TCA\u5faa\u74b0\u4e2d\u9593\u9ad4\u7684\u9176\u4fc3\u6a5f\u5236\uff0c\u56e0\u6b64OAA\u662f\u7d93\u7531\u4e19\u916e\u9178\u548c\u4e19\u916e\u9178\u7fa7\u6fc0\u9176\u7684\u7121\u53cd\u61c9\u9032\u5165\u9ede\u4e4b\u4e00\u3002 BioVision\u7684\u8349\u9170\u4e59\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u5feb\u901f\u7684\u5b9a\u91cf\u591a\u7a2e\u6a23\u54c1\u4e2dOAA\u7684\u65b9\u6cd5\u3002\u5728\u6e2c\u5b9a\u4e2d\uff0cOAA\u88ab\u8f49\u5316\u70ba\u4e19\u916e\u9178\uff0c\u4e19\u916e\u9178\u88ab\u7528\u65bc\u5c07\u5e7e\u4e4e\u7121\u8272\u7684\u63a2\u91dd\u8f49\u5316\u70ba\u6df1\u8272\uff08\u03bbmax= 570nm\uff09\u548c\u87a2\u5149\uff08Ex \/ Em = 535 \/ 587nm\uff09\u7522\u7269\u3002\u8349\u9170\u4e59\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u6aa2\u6e2c\u52300.1-10nmol\uff082-200 \u00b5M\uff09\u7684OAA\u3002\u3002\u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf\u00a0<\/strong>ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK659-100 (abcam ab83428)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (570 nm) or Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 \u00b5M) of OAA.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 40 minutes
\n\u2022 Fast and convenient
\n\u2022 Kit contains all necessary reagents for accurate measurement of OAA levels<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 OAA Assay Buffer
\n\u2022 OAA Probe ( in DMSO)
\n\u2022 OAA Enzyme Mix
\n\u2022 Developer
\n\u2022 OAA Standard (10 \u00b5mol)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0Home \u00a0 \u00a0Literature\u00a0 \u00a0Promotions\u00a0\u00a0Product Citations \u8349\u9170\u4e59 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1632],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24044"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24044"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24044\/revisions"}],"predecessor-version":[{"id":48491,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24044\/revisions\/48491"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24044"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24044"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24044"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}