{"id":23931,"date":"2020-03-08T14:26:47","date_gmt":"2020-03-08T06:26:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23931"},"modified":"2024-06-24T12:45:21","modified_gmt":"2024-06-24T04:45:21","slug":"%e8%84%82%e8%82%aa%e9%85%b8%e6%94%9d%e5%8f%96%e6%b8%ac%e5%ae%9a-fatty-acid-uptake-assay-kit-cell-based%e8%b2%a8%e8%99%9fk408-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=23931","title":{"rendered":"\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a | Fatty Acid Uptake Assay Kit (Cell-Based)\u8ca8\u865fK408-100 (ABCAM ab287857)"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=23931\">\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u7d30\u80de\uff09<strong> | <\/strong><strong>\u8ca8\u865f<\/strong>K408-100<\/a><\/span><\/p>\n<p>Fatty Acid Uptake Assay Kit (Cell-Based) (ABCAM ab287857)<\/p>\n<p>\u7522\u54c1\u4ecb\u7d39: \u8102\u80aa\u9178(Fatty acids)\u662f\u5fc5\u9700\u7684\u98f2\u98df\u71df\u990a\u7269\u8cea(essential dietary nutrients)\uff0c\u7531\u8207\u9577\u8102\u80aa\u65cf\u70f4\u93c8(long aliphatic hydrocarbon chain)\u76f8\u9023\u7684\u7fa7\u9178\u90e8\u5206\u7d44\u6210\u3002\u5b83\u5011\u662f\u66f4\u8907\u96dc\u7684\u8102\u8cea\uff08\u4f8b\u5982\u5f62\u6210\u7d30\u80de\u819c\u7684\u8102\u8cea\uff09\u7684\u57fa\u672c\u69cb\u5efa\u57fa\u584a\u3002\u5728\u8840\u6db2\u4e2d\uff0c\u9577\u93c8\u8102\u80aa\u9178\u8207\u8840\u6e05\u767d\u86cb\u767d(serum albumin)\u7d50\u5408\uff0c\u4e26\u901a\u904eSLC27\u8de8\u819c\u8f49\u904b\u86cb\u767d(transmembrane transporter proteins)\u5bb6\u65cf\u7684\u6210\u54e1\u8f49\u904b\u5230\u7d30\u80de\u4e2d\u3002\u8102\u80aa\u7d30\u80de\u651d\u53d6\u9577\u93c8\u6e38\u96e2\u8102\u80aa\u9178(adipocyte fatty acid uptake)\u5c0d\u65bc\u8abf\u7bc0\u5faa\u74b0\u8102\u8cea\u6c34\u5e73\u548c\u7d30\u80de\u8102\u8cea\u4ee3\u8b1d\u81f3\u95dc\u91cd\u8981\u3002\u8102\u80aa\u7d30\u80de\u8102\u80aa\u9178\u651d\u5165\u548c\u8102\u8cea\u4ee3\u8b1d\u7570\u5e38\u662f\u80a5\u80d6(obesity)\uff0c\u7cd6\u5c3f\u75c5(diabetes )\/\u4ee3\u8b1d\u7d9c\u5408\u5fb5(metabolic syndrome)\uff0c\u5fc3\u8840\u7ba1\u75be\u75c5(cardiovascular disorders)\u548c\u975e\u9152\u7cbe\u6027\u8102\u80aa\u809d\u75be\u75c5\uff08nonalcoholic fatty liver disease , NAFLD\uff09\u7684\u4e3b\u8981\u4fc3\u6210\u56e0\u7d20\u3002\u7531\u65bc\u98f2\u98df\u548c\u904b\u52d5\u5728\u5c0d\u6297\u80a5\u80d6\u65b9\u9762\u59cb\u7d42\u672a\u80fd\u53d6\u5f97\u4efb\u4f55\u6301\u4e45\u7684\u7d50\u679c\uff0c\u56e0\u6b64\u6b63\u5728\u7814\u7a76\u4ee5\u8102\u80aa\u9178\u70ba\u5438\u6536\u76ee\u6a19\u7684\u85e5\u7406\u5b78\u4f5c\u70ba\u4e00\u7a2e\u6f5b\u5728\u7684\u6cbb\u7642\u7b56\u7565\u3002 BioVision\u7684\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2(Fatty Acid Uptake Assay Kit)\u53ef\u5feb\u901f\u6e2c\u5b9a\u8868\u9054\u8102\u80aa\u9178\u8f49\u904b\u86cb\u767d\u7684\u6d3b\u7d30\u80de\u4e2d\u4e4b\u5373\u6642\u8102\u80aa\u9178\u651d\u53d6\u52d5\u529b\u5b78(real-time fatty acid uptake kinetics)\u3002\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u9ad8\u87a2\u5149\u9577\u93c8\u8102\u80aa\u9178\u985e\u4f3c\u7269\uff08Ex \/ Em = 488\/523 nm\uff09\uff0c\u8a72\u985e\u4f3c\u7269\u5145\u7576\u8de8\u819c\u8102\u80aa\u9178\u8f49\u904b\u86cb\u767d\uff08transmembrane fatty acid transporters , FATP\uff09\u7684\u5e95\u7269\u4e26\u7a4d\u805a\u5728\u7d30\u80de\u5167\u8102\u8cea\u5c0f\u6ef4\u4e2d\u3002\u5c08\u6709\u7684\u7121\u6bd2\u4e0d\u53ef\u6ef2\u900f\u819c\u6dec\u6ec5\u5291\u6d88\u9664\u4e86\u7d30\u80de\u5916\u7a7a\u9593\u7522\u751f\u7684\u4efb\u4f55\u87a2\u5149\uff0c\u5f9e\u800c\u78ba\u4fdd\u4e86\u7d30\u80de\u5167\u8102\u80aa\u9178\u7a4d\u7d2f\u7684\u7279\u5b9a\u6e2c\u91cf\uff0c\u800c\u7121\u9700\u4efb\u4f55\u7e41\u7463\u7684\u6d17\u6ecc\u6b65\u9a5f\u3002\u8a72\u6e2c\u5b9a\u6cd5\u9ad8\u5ea6\u9748\u654f\uff0c\u5177\u6709\u7c21\u55ae\u7684\u201cmix-and-read\u201d\u65b9\u6848\uff0c\u4e26\u4e14\u9ad8\u901a\u91cf\u9069\u7528\u65bc\u7be9\u9078\u8102\u80aa\u9178\u651d\u53d6\u8abf\u7bc0\u6027\u6e2c\u8a66\u5316\u5408\u7269\u6216\u6cbb\u7642\u689d\u4ef6\u3002\u8a72\u8a66\u5291\u76d2\u5305\u542b\u4e00\u5957\u5b8c\u6574\u7684\u8a66\u5291\uff0c\u8db3\u4ee5\u572896\u5b54\u677f\u4e2d\u9032\u884c100\u6b21\u53cd\u61c9<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abcam.com\/ps\/products\/287\/ab287857\/Images\/ab287857-477992-Fatty-Acid-Uptake-Assay-Kit.jpg\" \/><\/p>\n<div class=\"legend__title\"><span style=\"font-size: 12pt;\"><strong>\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong><\/span><\/div>\n<div class=\"legend__description\">\n<p>\u87a2\u5149\u6210\u50cf\u986f\u793a 60 \u5206\u9418\u5f8c\u87a2\u5149\u8102\u80aa\u9178\u985e\u4f3c\u7269\u7684\u5438\u6536\u7a0b\u5ea6\u3002 (i) \u672a\u5206\u5316\u76843T3-L1 \u7e96\u7dad\u6bcd\u7d30\u80de\uff0c(ii-iv) \u4ee5(ii) \u8f09\u9ad4\uff08\u542b0.1% DMSO \u7684\u57f9\u990a\u57fa\uff09\u3001(iii) 250 nM \u80f0\u5cf6\u7d20\u6216(iv) 250 nM \u80f0\u5cf6\u7d20\u548c5 \u03bcM \u9810\u8655\u7406\u7684\u5206\u53163T3-L1 \u8102\u80aa\u7d30\u80de\u6e25\u66fc\u9752\u9ef4\u7d20\u3002\u4f7f\u7528 Nikon TE2000 \u5012\u7f6e\u986f\u5fae\u93e1\u4f7f\u7528 20X Plan Fluor \u7269\u93e1\u548c FITC \u6ffe\u5149\u7247\u7acb\u65b9\u9ad4\u53d6\u5f97\u87a2\u5149\u5f71\u50cf\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abcam.com\/ps\/products\/287\/ab287857\/Images\/ab287857-477991-Fatty-Acid-Uptake-Assay-Kit.jpg\" \/><\/p>\n<div class=\"legend__title\"><span style=\"font-size: 12pt; color: #800000;\"><strong>\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong><\/span><\/div>\n<div class=\"legend__description\">\n<p>\u5728\u5b58\u5728\u5291\u91cf\u905e\u589e\u7684\u80f0\u5cf6\u7d20\uff080\u300125 \u548c 250 nM\uff09\u4ee5\u53ca\u80f0\u5cf6\u7d20\u548c\u6e25\u66fc\u9752\u9ef4\u7d20\u6df7\u5408\u7269\uff08250 nM + 5 \u03bcM\uff09\u7684\u60c5\u6cc1\u4e0b\uff0c3T3-L1 \u8102\u80aa\u7d30\u80de\u4e2d\u7684\u7d42\u9ede\u87a2\u5149\u3002\u52a0\u5165\u651d\u53d6\u53cd\u61c9\u6df7\u5408\u7269 60 \u5206\u9418\u5f8c\u6e2c\u91cf\u87a2\u5149\u3002\u6578\u64da\u70ba 4 \u500b\u7368\u7acb\u677f\u7684\u5e73\u5747\u503c RFU \u00b1 SEM\uff0c\u6bcf\u500b\u6578\u64da\u9ede\u4ee3\u8868\u56db\u500b\u5b54\u7684\u5e73\u5747\u503c\u3002<\/p>\n<\/div>\n<\/div>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K408-100 (ABCAM ab287857)<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Summary<\/strong><\/td>\n<td>\u2022 Detection method- Fluorescence (Ex\/Em = 488\/523 nm)<br \/>\n\u2022 Species reactivity- Mammalian<br \/>\n\u2022 Applications- This easy-to-use, one-step, non-radioactive kit can detect free fatty acid uptake using a variety of cell types.<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Fluorescence (Ex\/Em: 488\/523 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Eukaryotes<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>\u2022 Characterization of fatty acid uptake kinetics in live cells.<br \/>\n\u2022 Screening and characterization of drugs and test compounds for modulation of fatty acid uptake.<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Simple procedure (One Step)<br \/>\n\u2022 Fast and convenient<br \/>\n\u2022 The assay is easy, non-radioactive, and highly sensitive<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 Uptake Assay Buffer<br \/>\n\u2022 Extracellular Quenching Solution (100X)<br \/>\n\u2022 Fluorescent Fatty Acid Probe (200X)<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage Conditions<\/strong><\/td>\n<td>-20\u00b0C<\/td>\n<\/tr>\n<tr>\n<td><strong>Shipping Conditions<\/strong><\/td>\n<td>Gel Pack<\/td>\n<\/tr>\n<tr>\n<td><strong>USAGE<\/strong><\/td>\n<td>For Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u7d30\u80de\uff09 | \u8ca8\u865fK408-100 Fatty Acid Uptake Assay Kit [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":23932,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,5380,3],"tags":[1659],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23931"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23931"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23931\/revisions"}],"predecessor-version":[{"id":49692,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23931\/revisions\/49692"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/23932"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23931"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23931"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23931"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}