{"id":23855,"date":"2020-02-28T15:17:27","date_gmt":"2020-02-28T07:17:27","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23855"},"modified":"2023-02-14T14:26:45","modified_gmt":"2023-02-14T06:26:45","slug":"normal-goat-serum-southern-biotech%e8%b2%a8%e8%99%9f0060-01","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=23855","title":{"rendered":">>Normal Goat Serum | Southern Biotech\u8ca8\u865f0060-01"},"content":{"rendered":"

\u6b63\u5e38\u5c71\u7f8a\u8840\u6e05(<\/span><\/span>Normal Goat Serum)<\/span><\/span><\/strong> | <\/span><\/span><\/strong>\u8ca8\u865f<\/span><\/span><\/strong>0060-01<\/span><\/span><\/strong><\/a>\u898f\u683c<\/span><\/span><\/strong>10ML<\/span><\/span><\/span><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u6297\u9ad4\u7a2e\u985e\u7e41\u591a\u3001\u5177\u9ad8\u9748\u654f\u5ea6\u3002\u5176\u7522\u54c1\u7dda\u5305\u542b\u4e86\u5404\u5f0f\u5177\u6a19\u8a8c\u7684\u4e8c\u7d1a\u6297\u9ad4\u3001\u514d\u75ab\u7403\u86cb\u767d\u3001\u52d5\u7269\u8840\u6e05\u7b49\u7522\u54c1\uff0c\u53ef\u61c9\u7528\u65bcBlotting\u3001Control \u3001ELISA\u3001Flow cytometry\u3001Immunohistochemistry\u3001 IF\u3001Immunoprecipitation\u7b49\u7814\u7a76\u3002<\/span><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39: <\/span><\/span><\/strong>\u5c01\u9589\u8840\u6e05(Blocking serum)\u5ee3\u6cdb\u61c9\u7528\u65bc\u514d\u75ab\u53cd\u61c9\u904e\u7a0b\u4e2d\uff0c\u963b\u65b7(blocking)\u7d44\u7e54\u548c\u7d30\u80de\u975e\u7279\u7570\u6027\u6297\u9ad4\u7d50\u5408\uff0c\u800c\u964d\u4f4e\u975e\u7279\u7570\u6027\u7d50\u5408\u6240\u9020\u6210\u7684\u80cc\u666f\u503c\u3002\u5c01\u9589\u8840\u6e05(Blocking serum) \u963b\u65b7\u4e86\u6a23\u54c1\u4e2dFc\u53d7\u9ad4(Fc receptors)\u8207\u5be6\u9a57\u4e2d\u4f7f\u7528\u7684\u4e00\u6297\u548c\u4e8c\u6297\u7684\u7d50\u5408\uff0c\u4e26\u4e14\u963b\u65b7\u4e86\u6297\u9ad4\u8207\u6a23\u54c1\u7684\u975e\u7279\u7570\u6027\u7d50\u5408(non-specific binding)\u3002\u901a\u5e38\uff0c\u7528\u65bc\u5c01\u9589\u7684\u8840\u6e05\u4f86\u81ea\u8207\u7522\u751f\u7b2c\u4e00\u6297\u9ad4\u7684\u7269\u7a2e\u4e0d\u540c\u7684\u7269\u7a2e\u3002\u5c01\u9589\u6027\u8840\u6e05\u901a\u5e38\u4f86\u81ea\u7522\u751f\u7b2c\u4e8c\u6297\u9ad4\u7684\u7269\u7a2e\u3002\u53ef\u61c9\u7528\u65bcwestern blot\u3001 ELISA\u3001IHC\u3001Flow\u5206\u6790\uff0c\u964d\u4f4e\u975e\u7279\u7570\u6027\u6297\u9ad4\u7d50\u5408\u3002\u8840\u6e05\u53ef\u76f4\u63a5\u7528\u65bcblocking\uff0c\u6216\u4f5c\u70bablocking buffer\u7684\u6210\u5206\u3002\u5e38\u898b\u7684\u5c01\u9589\u8840\u6e05\u6709\u6b63\u5e38\u9a62\u8840\u6e05(Normal\u00a0donkey\u00a0serum)\u3001\u6b63\u5e38\u5154\u8840\u6e05\u3001(Normal\u00a0Rabbit\u00a0serum) \u3001\u6b63\u5e38\u8001\u9f20\u8840\u6e05(Normal\u00a0Mouse\u00a0serum)\u3001\u6b63\u5e38\u5c71\u7f8a\u8840\u6e05(Normal\u00a0Goat\u00a0serum)\u3002<\/span><\/span><\/p>\n

\"\"\"\"<\/p>\n

\u4eba\u80f0\u817a\u764c\u7d30\u80de\u7cfb MIA PaCa-2 \u7528 10% \u6b63\u5e38\u5c71\u7f8a\u8840\u6e05\uff08SB \u76ee\u9304\u865f 0060-01\uff09\u5c01\u9589\uff0c\u4e26\u7528\u5c0f\u9f20\u6297\u7d30\u80de\u89d2\u86cb\u767d 18-UNLB\uff08SB \u76ee\u9304\u865f 10085-01\uff1b\u53f3\uff09\u67d3\u8272\uff0c\u96a8\u5f8c\u901a\u904e\u5c71\u7f8a\u6297\u5c0f\u9f20 IgG(H+L)\u3001\u4eba ads-AF555\uff08SB \u76ee\u9304\u865f 1031-32\uff09\u548c DAPI<\/span><\/span><\/p>\n

\u7522\u54c1\u7279\u6027: \u6b63\u5e38\u5c71\u7f8a\u8840\u6e05(Normal Goat Serum)\uff0c\u4f86\u6e90\u70baAdult mixed breed goats\u3002\u8655\u7406\u904e\u7a0b\u4ee5\u6e1b\u5c11\u6eb6\u8840\u60c5\u6cc1\uff0c\u4e26\u4ee50.2\u03bcm filter\u904e\u6ffe\u3002\u8840\u6e05\u6eb6\u6db2\u5305\u542b0.1% sodium azide\u3002\u8caf\u5b58\u65bc2-8\u00b0C\u3002\u9577\u671f\u4fdd\u5b58-20\u00b0C<\/span><\/span><\/p>\n\n\n\n\n\n\n\n
\u00a0\u8c93\u3002<\/span>\u4e0d\u3002<\/span><\/span><\/strong><\/td>\n\u7522\u54c1\u63cf\u8ff0<\/span><\/span><\/strong><\/td>\n\u514b\u9686<\/span><\/span><\/strong><\/td>\n\u5c3a\u5bf8<\/span><\/span><\/strong><\/td>\n<\/tr>\n
0030-01<\/span><\/span><\/strong><\/a><\/td>\n\u6b63\u5e38\u9a62\u8840\u6e05<\/span><\/span><\/a>\u00a0(<\/span><\/span>Normal Donkey Serum)<\/span><\/span><\/strong><\/td>\n<\/td>\n10\u6beb\u5347<\/span><\/span><\/td>\n<\/tr>\n
0040-01<\/span><\/span><\/strong><\/a><\/td>\n\u6b63\u5e38\u5154\u8840\u6e05<\/span><\/span><\/a>\u00a0( <\/span><\/span>Normal Rabbit Serum)<\/span><\/span><\/strong><\/td>\n<\/td>\n5\u6beb\u5347<\/span><\/span><\/td>\n<\/tr>\n
0050-01<\/span><\/span><\/strong><\/a><\/td>\n\u6b63\u5e38\u8001\u9f20\u8840\u6e05<\/span><\/span><\/a>\u00a0( <\/span><\/span>Normal Mouse Serum)<\/span><\/span><\/strong><\/td>\n<\/td>\n5\u6beb\u5347<\/span><\/span><\/td>\n<\/tr>\n
0060-01<\/span><\/span><\/strong><\/a><\/td>\n\u6b63\u5e38\u5c71\u7f8a\u8840\u6e05<\/span><\/span><\/a>\u00a0( <\/span><\/span>Normal Goat Serum)<\/span><\/span><\/strong><\/td>\n<\/td>\n10\u6beb\u5347<\/span><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

SBA<\/span><\/span><\/strong>\u71b1\u92b7\u5546\u54c1<\/span><\/span><\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/span><\/span><\/a>\u00a0|\u00a0<\/span><\/span>monocloal\u00a0isotyping\u5957\u7d44<\/span><\/span><\/a>\u00a0|\u00a0<\/span><\/span>Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/span><\/span><\/a><\/u>\u00a0|\u00a0<\/span><\/span><\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/span><\/span><\/a>\u00a0|<\/span><\/span><\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/span><\/a>|\u00a0<\/span><\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/span><\/a>\u00a0| <\/span><\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/span><\/a>| <\/span>\u4e00\u7d1a\u6297\u9ad4<\/span><\/a><\/span>\u00a0<\/u>\u00a0<\/u><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u6b63\u5e38\u5c71\u7f8a\u8840\u6e05(Normal Goat Serum) | \u8ca8\u865f0060-01\u898f\u683c10ML \u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Sout […]<\/p>\n","protected":false},"author":3,"featured_media":6751,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1673],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23855"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23855"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23855\/revisions"}],"predecessor-version":[{"id":38343,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23855\/revisions\/38343"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6751"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23855"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23855"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23855"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}