{"id":23546,"date":"2020-02-07T17:41:50","date_gmt":"2020-02-07T09:41:50","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23546"},"modified":"2026-04-01T17:56:47","modified_gmt":"2026-04-01T09:56:47","slug":"amp%e5%96%ae%e7%a3%b7%e9%85%b8%e8%85%ba%e8%8b%b7%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90-%e8%b2%a8%e8%99%9fk229-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=23546","title":{"rendered":"AMP\u55ae\u78f7\u9178\u817a\u82f7\u5448\u8272\u5206\u6790 | \u8ca8\u865fK229-100 (ABCAM ab273275)"},"content":{"rendered":"

AMP\u55ae\u78f7\u9178\u817a\u82f7\u5448\u8272\u5206\u6790 | \u8ca8\u865f<\/strong>K229-100 (ABCAM ab273275)<\/a><\/span><\/p>\n

AMP Colorimetric Assay Kit<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u55ae\u78f7\u9178\u817a\u82f7\uff08Adenosine Monophosphate , AMP\uff09\uff0c\u4e5f\u7a31\u70ba5′-\u817a\u82f7\u9178 (5\u2019-adenylic acid,)\uff0c\u7531\u817a\u560c\u5464(adenine)\uff0c\u6838\u7cd6(ribose)\u548c\u78f7\u9178\u9e7d(phosphate)\u7d44\u6210\u3002\u7531\u65bcATP \/ ADP\u7684\u6c34\u89e3\u4f5c\u7528\u6216\u7576RNA\u5206\u89e3\u6642\uff0c\u901a\u904e\u7d50\u5408\u5169\u500bADP\u5206\u5b50\uff0c\u817a\u82f7\u9178\u6fc0\u9176(adenylate kinase)\u53ef\u5728ATP\u5408\u6210\u904e\u7a0b\u4e2d\u7522\u751fAMP\u3002 AMP\u5728\u8a31\u591a\u7d30\u80de\u4ee3\u8b1d\u904e\u7a0b\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\uff0c\u4f8b\u5982Ca2 +\u4fe1\u865f\u50b3\u5c0e(Ca2+ signaling)\uff0c\u7d30\u80de\u9077\u79fb(cell migration)\uff0c\u7d30\u80de\u56e0\u5b50\u5206\u6ccc(cytokine secretion)\u7b49\u3002AMP\u662fAMP\u6fc0\u6d3b\u7684\u86cb\u767d\u6fc0\u9176\u7684\u6fc0\u6d3b\u5291\uff0c\u5b83\u8abf\u7bc0\u8461\u8404\u7cd6\u7684\u651d\u53d6(glucose uptake)\uff0c\u8102\u80aa\u9178\u7684\u651d\u53d6(fatty acid uptake)\uff0c\u8102\u80aa\u9178\u7684\u6c27\u5316(fatty acid oxidation)\u7b49\u3002AMP\u7684\u6c34\u5e73\u53ef\u4ee5\u901a\u904e\u87a2\u5149\u7d20\u9176(luciferase )\/\u87a2\u5149\u7d20(luciferin)\u4ecb\u5c0e\u7684\u6e2c\u5b9a\u4f86\u6e2c\u91cf\u3002\u4f46\u662f\uff0c\u87a2\u5149\u7d20\u9176\u4fe1\u865f\u4e0d\u7a69\u5b9a\uff0c\u5927\u591a\u6578\u5be6\u9a57\u5ba4\u901a\u5e38\u6c92\u6709luminescent\u51b7\u5149\u8a2d\u5099\u3002 BioVision\u7684AMP\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 (AMP Colorimetric Assay Kit) \u63d0\u4f9b\u4e86\u4e00\u7a2e\u65b9\u4fbf\u7684\u65b9\u6cd5\u4f86\u6aa2\u6e2c\u751f\u7269\u6a23\u54c1\u4e2d\u7684AMP\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u5728\u7126\u78f7\u9178\u9e7d(Pyrophosphate)\u548c\u78f7\u9178\u70ef\u9187\u4e19\u916e\u9178(Phosphoenolpyruvate)\u5b58\u5728\u4e0b\uff0cAMP\u88ab\u8f49\u5316\u70ba\u4e19\u916e\u9178(pyruvate)\u3002\u96a8\u5f8c\u9032\u884c\u4e00\u7cfb\u5217\u7684\u9176\u4fc3\u53cd\u61c9(enzymatic reactions)\uff0c\u751f\u6210\u6709\u8272\u7522\u7269\uff0c\u5728570 nm\u8655\u5177\u6709\u5f88\u5f37\u7684\u5438\u5149\u5ea6\u3002\u5438\u5149\u5ea6\u8207\u6a23\u54c1\u4e2dAMP\u7684\u542b\u91cf\u6210\u6b63\u6bd4\u3002\u8a72\u5957\u4ef6\u5feb\u901f\uff0c\u9748\u654f\uff0c\u6613\u65bc\u4f7f\u7528\u4e14\u5177\u6709\u9ad8\u901a\u91cf\u9069\u61c9\u6027\u3002\u5b83\u53ef\u4ee5\u6e2c\u91cf\u5404\u7a2e\u6a23\u54c1\u985e\u578b\u4e2d\u4f4e\u65bc10 \u00b5M\u7684AMP\u6c34\u5e73\u3002<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK229-100 (ABCAM ab273275)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nBioVision\u2019s AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 \u00b5M in various sample types.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance, OD 570 nm<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nAll<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Study the regulation of AMPK by AMP
\n\u2022 Mechanistic study of key cellular processes such as Ca2+ signaling, glucose uptake, lipid uptake, etc.
\n\u2022 Measurement of AMP level in various cell or tissue types<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Sensitive
\n\u2022 High-throughput adaptable
\n\u2022 It can measure AMP level lower than 10 \u00b5M in various sample types
\n\u2022 Easy to use
\n\u2022 Rapid<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 AMP Standard
\n\u2022 AMP Enzyme
\n\u2022 AMP Assay Buffer
\n\u2022 AMP Substrate Mix
\n\u2022 AMP Developer
\n\u2022 AMP Probe<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

AMP\u55ae\u78f7\u9178\u817a\u82f7\u5448\u8272\u5206\u6790 | \u8ca8\u865fK229-100 (ABCAM ab273275) AMP Colorime […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,3],"tags":[1693],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23546"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23546"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23546\/revisions"}],"predecessor-version":[{"id":54545,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23546\/revisions\/54545"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23546"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23546"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23546"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}