{"id":23524,"date":"2020-02-07T12:03:49","date_gmt":"2020-02-07T04:03:49","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23524"},"modified":"2022-07-19T17:34:25","modified_gmt":"2022-07-19T09:34:25","slug":"sars-cov-spike-protein-%e5%8c%97%e4%ba%ac2002-beijing02-%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-293-cell-%e8%b2%a8%e8%99%9fit-002-001p","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=23524","title":{"rendered":"SARS-CoV Spike \u91cd\u7d44\u86cb\u767d | \u5317\u4eac2002, Beijing02 \u8ca8\u865fIT-002-001p"},"content":{"rendered":"

SARS-CoV Spike protein (1-1190)(\u5317\u4eac2002, Beijing02)\u91cd\u7d44\u86cb\u767d (293 cell) | <\/strong>\u8ca8\u865f<\/strong>IT-002-001p<\/a><\/span><\/p>\n

S (1-1190)(Beijing02)<\/strong> (amino acid 1-1190)<\/p>\n

\"\"<\/p>\n

\u51a0\u72c0\u75c5\u6bd2\u4ecb\u7d39<\/p>\n

\u51a0\u72c0\u75c5\u6bd2\uff08Coronavirus [CoV]\uff09\u70ba\u5177\u5916\u5957\u819c\uff08envelope\uff09\u7684\u75c5\u6bd2\uff0c\u5728\u96fb\u5b50\u986f\u5fae\u93e1\u4e0b\u53ef\u770b\u5230\u985e\u4f3c\u7687\u51a0\u7684\u7a81\u8d77\u56e0\u6b64\u5f97\u540d\u3002\u4eba\u985e\u611f\u67d3\u51a0\u72c0\u75c5\u6bd2\u4ee5\u547c\u5438\u9053\u75c7\u72c0\u70ba\u4e3b\uff0c\u4f46\u56b4\u91cd\u6025\u6027\u547c\u5438\u9053\u75c7\u5019\u7fa4\u51a0\u72c0\u75c5\u6bd2\uff08SARS-CoV\uff09\u3001\u4e2d\u6771\u547c\u5438\u75c7\u5019\u7fa4\u51a0\u72c0\u75c5\u6bd2\uff08MERS-CoV\uff09\u8207\u65b0\u578b\u51a0\u72c0\u75c5\u6bd2\uff082019-nCoV\uff09\u611f\u67d3\u5f8c\u6bd4\u4e00\u822c\u4eba\u985e\u51a0\u72c0\u75c5\u6bd2\u75c7\u72c0\u56b4\u91cd\uff0c\u90e8\u5206\u500b\u6848\u53ef\u80fd\u51fa\u73fe\u56b4\u91cd\u7684\u80ba\u708e\u8207\u547c\u5438\u8870\u7aed\u7b49\u3002\u51a0\u72c0\u75c5\u6bd2\u6703\u5f15\u8d77\u4eba\u985e\u548c\u810a\u690e\u52d5\u7269\u7684\u75be\u75c5\uff0c\u5c6c\u65bc\u4eba\u755c\u5171\u901a\u50b3\u67d3\u75be\u75c5\u3002\u5927\u90e8\u5206\u7684\u4eba\u985e\u51a0\u72c0\u75c5\u6bd2\u90fd\u662f\u56e0\u70ba\u76f4\u63a5\u63a5\u89f8\u5230\u5e36\u6709\u75c5\u6bd2\u7684\u5206\u6ccc\u7269\u6216\u98db\u6cab\u50b3\u67d3\u70ba\u4e3b\u3002\u6709\u90e8\u5206\u52d5\u7269\u7684\u51a0\u72c0\u75c5\u6bd2\u6703\u8b93\u52d5\u7269\u51fa\u73fe\u8179\u7009\u75c7\u72c0\uff0c\u53ef\u4ee5\u5728\u7cde\u4fbf\u7576\u4e2d\u627e\u5230\u75c5\u6bd2\uff0c\u56e0\u6b64\u4e5f\u6709\u53ef\u80fd\u85c9\u7531\u63a5\u89f8\u5230\u611f\u67d3\u52d5\u7269\u7684\u7cde\u4fbf\u800c\u50b3\u64ad\u30022002\u5e74\u767c\u73fe\u7684\u56b4\u91cd\u6025\u6027\u547c\u5438\u9053\u75c7\u5019\u7fa4\uff08SARS\uff09\u8207\u63a5\u89f8\u5230\u51a0\u72c0\u75c5\u6bd2\u611f\u67d3\u4e4b\u9e9d\u9999\u8c93\u6216\u8759\u8760\u7b49\u52d5\u7269\u53ef\u80fd\u6709\u95dc\uff1b2012\u5e74\u767c\u73fe\u7684\u4e2d\u6771\u547c\u5438\u75c7\u5019\u7fa4\u51a0\u72c0\u75c5\u6bd2\u611f\u67d3\u75c7\uff08MERS\uff09\u8207\u63a5\u89f8\u99f1\u99dd\u6216\u98f2\u7528\u99f1\u99dd\u5976\u6709\u95dc\u3002\u8cc7\u6599\u4f86\u6e90CDC\u3002<\/p>\n

\u51a0\u72c0\u75c5\u6bd2(Coronaviruses)\u662f\u5c6c\u4e8e\u51a0\u72c0\u75c5\u6bd2\u79d1\uff08\u51a0\u72c0\u75c5\u6bd2\u79d1\uff09\u4e2d\u51a0\u72c0\u75c5\u6bd2\u4e9e\u79d1\u7684\u75c5\u6bd2\u7a2e\uff0c\u9806\u5e8f\u70baNidovirales\u3002 \u51a0\u72c0\u75c5\u6bd2(\u00a0Coronaviruses)\u662f\u5177\u6709\u6b63\u5411\u55ae\u93c8RNA\u57fa\u56e0\u7d44(\u00a0positive-sense single-stranded RNA genome )\u4e14\u5177\u6709\u87ba\u65cb\u5c0d\u7a31\u6838\u8863\u6bbc\u7684\u5305\u819c\u75c5\u6bd2(enveloped viruses)\u3002 \u69cb\u6210\u6240\u6709\u51a0\u72c0\u75c5\u6bd2\u6574\u9ad4\u7d50\u69cb\u7684\u86cb\u767d\u8cea\u662f\u523a\u7a81\uff08\u00a0spike, S\uff09\uff0c\u5305\u819c\uff08envelope, E\uff09\uff0c\u819c\uff08membrane, M\uff09\u548c\u6838\u8863\u6bbc\uff08nucleocapsid , N\uff09\u3002 \u5728SARS\u51a0\u72c0\u75c5\u6bd2\u7684\u7279\u5b9a\u60c5\u6cc1\u4e0b\uff0cS\u4e0a\u5b9a\u7fa9\u7684\u53d7\u9ad4\u7d50\u5408\u7d50\u69cb\u57df\u4ecb\u5c0e\u75c5\u6bd2\u8207\u5176\u7d30\u80de\u53d7\u9ad4(cellular receptor) \u8840\u7ba1\u7dca\u5f35\u7d20\u8f49\u5316\u91762\uff08angiotensin-converting enzyme 2 , ACE2\uff09\u7684\u7d50\u5408\u3002 \u4e00\u4e9b\u51a0\u72c0\u75c5\u6bd2\uff1b\u7279\u5225\u662fBeta\u51a0\u72c0\u75c5\u6bd2\u4e9e\u7d44A (\u00a0Betacoronavirus subgroup A) \u7684\u6210\u54e1\uff0c\u4e5f\u5177\u6709\u7a31\u70ba\u8840\u51dd\u7d20\u916f\u9176\uff08hemagglutinin esterase, HE\uff09\u7684\u8f03\u77ed\u7684\u00a0spike-like\u86cb\u767d\u3002<\/p>\n

\u7522\u54c1<\/strong>\u540d\u7a31<\/strong>:<\/strong> S (1-1190)(Beijing02)\u91cd\u7d44\u86cb\u767d\uff0cSARS-CoV (\u5317\u4eac2002) Spike protein (\u80fa\u57fa1-1190) C-terminal 6xHis tagged (Genebank Accession No. AY278487)\uff1b\u53ef\u61c9\u7528WB standard,\u7b49\u5be6\u9a57\u3002 \u7d14\u5ea6\u5927\u65bc95%\u3002Each vial contains 100 \u00b5g of lyophilized protein (1 mg\/ml) in PBS. Reconstitute the protein with 100\u00b5l of Millipore water.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n

\nProduct Name:<\/strong><\/td>\n		S (1-1190)(Beijing02)<\/strong><\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
Cat No.:<\/strong><\/td>\nIT-002-001p<\/td>\n<\/tr>\n
Source:<\/strong><\/td>\nViral protein purified from 293 cell culture<\/td>\n<\/tr>\n
Formulation:<\/strong><\/td>\nEach vial contains 100 \u00b5g purified protein (1 mg\/ml) in PBS.<\/td>\n<\/tr>\n
Product Type:<\/strong><\/td>\nPurified Protein<\/td>\n<\/tr>\n
Protein:<\/strong><\/td>\n6xHis tagged Spike protein (amino acid 1-1190) (SARS-CoV) (Beijing02) (Genebank No. AY278487)<\/td>\n<\/tr>\n
Applications:<\/strong><\/td>\nWB,etc<\/td>\n<\/tr>\n
Specificity:<\/strong><\/td>\n>= 95% purity<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0Chloroquine Diphosphate<\/a>\u00a0|\u00a0MK-1439\/ Doravirine<\/a>\u00a0|\u00a0SARS-CoV Spike protein\u00a0<\/a>\u00a0|\u00a0MERS-Cov Spike protein<\/a>\u00a0|\u00a0\u4f0a\u6ce2\u62c9glycoprotein<\/a><\/span><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/strong><\/td>\nDescription<\/strong><\/td>\nProduct Type<\/strong><\/td>\nApplications<\/strong><\/td>\n<\/tr>\n
IT-002-001p<\/a><\/td>\nS (1-1190)(Beijing02)<\/a><\/td>\nPurified Protein<\/a><\/td>\nWB,etc<\/td>\n<\/tr>\n
IT-002-010p<\/a><\/td>\nSpike Protein (MERS-CoV\/EMC 2C\/2012)<\/a><\/td>\nPurified Protein<\/a><\/td>\nWB standard, antibody ELISA, etc<\/td>\n<\/tr>\n
IT-002-051Ep<\/a><\/td>\nSARS Core (1-49 a.a.)<\/a><\/td>\nE.coli Protein<\/a><\/td>\nWB standard, antibody ELISA, immunogen, etc.<\/td>\n<\/tr>\n
IT-002-052Ep<\/a><\/td>\nSARS Core (340-390 a.a.)<\/a><\/td>\nE.coli Protein<\/a><\/td>\nWB standard, antibody ELISA, immunogen, etc.<\/td>\n<\/tr>\n
IT-002-053Ep<\/a><\/td>\nSARS Envelope<\/a><\/td>\nE.coli Protein<\/a><\/td>\nWB standard, antibody ELISA, immunogen, etc. Specificity: >95% , based on SDS PAGE<\/td>\n<\/tr>\n
IT-002-054Ep<\/a><\/td>\nSARS Matrix<\/a><\/td>\nE.coli Protein<\/a><\/td>\nWB standard, antibody ELISA, immunogen, etc.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

SARS-CoV Spike protein (1-1190)(\u5317\u4eac2002, Beijing02)\u91cd\u7d44\u86cb\u767d […]<\/p>\n","protected":false},"author":3,"featured_media":23526,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1699,1698],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23524"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23524"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23524\/revisions"}],"predecessor-version":[{"id":23545,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23524\/revisions\/23545"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/23526"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23524"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23524"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23524"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}