{"id":23477,"date":"2020-02-06T13:21:01","date_gmt":"2020-02-06T05:21:01","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23477"},"modified":"2022-07-19T17:35:26","modified_gmt":"2022-07-19T09:35:26","slug":"anti-myc-tag-antibody-%e8%b2%a8%e8%99%9fg077","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=23477","title":{"rendered":"Anti-Myc Tag Antibody | \u8ca8\u865fG077"},"content":{"rendered":"

Anti-Myc Tag Antibody | \u8ca8\u865fG077<\/a><\/span><\/p>\n

\u8868\u4f4d\u6a19\u7c64(Epitope tags)\u662f\u77ed\u80bd\u5e8f\u5217(short peptide sequences)\uff0c\u6613\u65bc\u88ab\u6a19\u7c64\u7279\u7570\u6027\u6297\u9ad4\u8b58\u5225(tag-specific antibodies)\u3002 \u7531\u65bc\u8868\u4f4d\u6a19\u7c64\u5f88\u5c0f\uff0c\u56e0\u6b64\u4e0d\u6703\u5f71\u97ff\u6a19\u7c64\u86cb\u767d\u7684\u751f\u5316\u7279\u6027(biochemical properties)\u3002\u5728\u514b\u9686\u6642\uff0c\u7de8\u78bc\u8868\u4f4d\u6a19\u7c64\u7684\u5e8f\u5217\u6700\u5e38\u5305\u542b\u5728\u76ee\u6a19DNA\u4e2d\uff0c\u4ee5\u7522\u751f\u5305\u542b\u8868\u4f4d\u6a19\u7c64\u5e8f\u5217(epitope tag sequence)\u7684\u878d\u5408\u86cb\u767d\u3002 \u9019\u4f7f\u5f97\u6297\u8868\u4f4d\u6a19\u7c64\u6297\u9ad4(anti-epitope tag antibodies)\u53ef\u4ee5\u7528\u4f5c\u901a\u7528\u6aa2\u6e2c\u8a66\u5291\uff0c\u7528\u65bc\u901a\u904e\u91cd\u7d44\u65b9\u6cd5\u7522\u751f\u7684\u4efb\u4f55\u5305\u542b\u6a19\u7c64\u7684\u86cb\u767d\u8cea\u3002 \u9019\u610f\u5473\u8457\u6297\u8868\u4f4d\u6a19\u7c64\u6297\u9ad4\u662f\u7522\u751f\u7279\u7570\u6027\u6297\u9ad4\u4ee5\u9451\u5b9a\uff0c\u514d\u75ab\u6c89\u6fb1(immunoprecipitate)\u6216\u514d\u75ab\u89aa\u548c\u7d14\u5316(immunoaffinity purify)\u91cd\u7d44\u86cb\u767d\u7684\u6709\u7528\u66ff\u4ee3\u65b9\u6cd5\u3002\u7522\u751f\u8868\u4f4d\u6a19\u7c64\u878d\u5408\u86cb\u767d\u7684\u8868\u9054\u8f09\u9ad4\u53ef\u7528\u65bc\u591a\u7a2e\u5bbf\u4e3b\u8868\u9054\u7cfb\u7d71\uff0c\u5305\u62ec\u7d30\u83cc(bacteria)\uff0c\u9175\u6bcd(yeast)\uff0c\u6606\u87f2(insect)\u548c\u54fa\u4e73\u52d5\u7269\u7d30\u80de(mammalian cells)\u3002<\/p>\n

\"Functionality<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/strong><\/td>\nAnti-Myc Tag Antibody (Rabbit Polyclonal)<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve a universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.<\/td>\n<\/tr>\n
Subtype<\/strong><\/td>\ntag<\/td>\n<\/tr>\n
Application<\/strong><\/td>\nWB, ELISA<\/td>\n<\/tr>\n
Recommended Dilution<\/strong><\/td>\nELISA 1:1,000 ; WESTERN BLOT 1:500 – 1:1,000 ; IMMUNOHISTOCHEMISTRY User Optimized ; OTHER APPLICATIONS User Optimized<\/td>\n<\/tr>\n
Raised In<\/strong><\/td>\nRabbit<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nThis polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.<\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nMyc epitope tag peptide E-Q-K-L-I-S-E-E-D-L<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Quantity<\/strong><\/td>\n100 \u03bcg<\/td>\n<\/tr>\n
Clone<\/strong><\/td>\nPolyclonal<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nThis product is stable for several weeks at 4\u00b0C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20\u00b0C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\nPBS, pH 7.4 with 0.05% sodium azide.<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1 mg\/ml<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\nAffinity purified<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nIgG<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nother<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Anti-Myc Tag Antibody | \u8ca8\u865fG077 \u8868\u4f4d\u6a19\u7c64(Epitope tags)\u662f\u77ed\u80bd\u5e8f\u5217( […]<\/p>\n","protected":false},"author":3,"featured_media":5254,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[476],"tags":[1706],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23477"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23477"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23477\/revisions"}],"predecessor-version":[{"id":23478,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23477\/revisions\/23478"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5254"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23477"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23477"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23477"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}