{"id":23154,"date":"2020-01-20T16:29:48","date_gmt":"2020-01-20T08:29:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23154"},"modified":"2022-07-19T17:38:45","modified_gmt":"2022-07-19T09:38:45","slug":"sumo%e8%9b%8b%e7%99%bd%e9%85%b61%ef%bc%88his%ef%bc%89%e9%85%b5%e6%af%8d%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9f7868-500","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=23154","title":{"rendered":"SUMO\u86cb\u767d\u91761\uff08His\uff09\u9175\u6bcd\u91cd\u7d44\u86cb\u767d| \u8ca8\u865f7868-500"},"content":{"rendered":"

SUMO\u86cb\u767d\u91761\uff08His\uff09\u9175\u6bcd\u91cd\u7d44\u86cb\u767d| <\/strong>\u8ca8\u865f<\/strong>7868-500<\/a><\/span><\/p>\n

EZCut\u2122 SUMO Protease 1 (His-tagged), Yeast Recombinant<\/p>\n

\u7522\u54c1\u4ecb\u7d39: SUMO\uff08Small Ubiquitin-like Modifiers, \u5c0f\u6cdb\u7d20\u6a23\u4fee\u98fe\u7269\uff09\u86cb\u767d\u91761\uff08Protease 1, Ulp1\uff0c\u4f86\u81ea\u91c0\u9152\u9175\u6bcd\u7684Ubl\u7279\u7570\u6027\u86cb\u767d\u91761\uff09\u662f\u4e00\u7a2e\u9ad8\u6d3b\u6027\u7684\u534a\u80f1\u6c28\u9178\u86cb\u767d\u9176(cysteine protease)\u3002\u5b83\u5177\u6709\u5f88\u9ad8\u7684\u7279\u7570\u6027\uff0c\u56e0\u70ba\u5b83\u8b58\u5225\u6cdb\u7d20\u6a23\uff08UBL\uff09\u86cb\u767dSUMO\uff08Smt3\uff09\u7684\u4e09\u7d1a\u7d50\u69cb(tertiary structure)\uff0c\u800c\u4e0d\u662f\u5176\u6c28\u57fa\u9178\u5e8f\u5217(amino acid sequence)\u3002 SUMO fusion tag\u4f5c\u70baN\u672b\u7aef\u878d\u5408(N-terminal fusion partner)\uff0c\u5df2\u986f\u793a\u51fa\u53ef\u4ee5\u986f\u8457\u63d0\u9ad8\u86cb\u767d\u8cea\u7684\u7a69\u5b9a\u6027\u548c\u6eb6\u89e3\u6027\u4f86\u589e\u5f37\u539f\u6838(prokaryotic)\u548c\u771f\u6838(eukaryotic)\u8868\u9054\u7cfb\u7d71\u4e2d\u529f\u80fd\u6027\u86cb\u767d\u8cea\u7684\u7522\u751f\u3002\u542b\u6709N\u7aefHis\u6a19\u7c64\u7684\u91cd\u7d44EZCut\u2122SUMO\u86cb\u767d\u91761\u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\uff0c\u4e26\u901a\u904e\u5c08\u6709\u8272\u8b5c\u6280\u8853(chromatographic techniques)\u7d14\u5316\u3002 EZCut\u2122SUMO\u86cb\u767d\u91761\u53ef\u7528\u65bc\u5f9e\u91cd\u7d44SUMO\u878d\u5408\u86cb\u767d\u4e0a\u5207\u5272SUMO\u86cb\u767d\u6a19\u7c64\u3002\u88c2\u89e3\u7684\u6700\u4f73\u6eab\u5ea6\u70ba30\u00b0C\uff1b\u4f46\u662f\uff0c\u8a72\u9176\u5728\u5f88\u5bec\u7684\u6eab\u5ea6\u548cpH\u503c\u7bc4\u570d\u5167\u90fd\u5177\u6709\u6d3b\u6027\u3002\u88c2\u89e3\u53cd\u61c9\u5b8c\u6210\u5f8c\uff0c\u53ef\u4ee5\u901a\u904e\u4f7f\u7528\u93b3\u87af\u5408\u6a39\u8102(Ni chelating resin)\uff08\u8ca8\u865f6562-1\u300110\u3001100\u3001500\uff09\u7684\u89aa\u548c\u8272\u8b5c\u6cd5(affinity chromatography)\u8f15\u9b06\u5730\u5f9e\u53cd\u61c9\u4e2d\u53bb\u9664\u86cb\u767d\u9176\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/strong><\/td>\nUlp1 peptidase, SUMO Protease<\/td>\n<\/tr>\n
Gene Symbol<\/strong><\/td>\nULP1<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n856087<\/td>\n<\/tr>\n
Accession #<\/strong><\/td>\nQ02724<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nE. coli<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Physical Form Description<\/strong><\/td>\n0.1 mg\/ml (100 U\/\u00b5l) solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n28.7 kDa (403-621 aa + N-terminal Poly-His tag).<\/td>\n<\/tr>\n
Purity by SDS-PAGE<\/strong><\/td>\n\u226590%<\/td>\n<\/tr>\n
Biological Activity<\/strong><\/td>\nThe EZCut\u2122 SUMO Protease 1 (His-tagged) has an activity of \u2265 1 X 106 units\/mg.<\/td>\n<\/tr>\n
Amino Acid Sequence<\/strong><\/td>\n403-621 aa + N-terminal Poly-His tag<\/td>\n<\/tr>\n
Handling<\/strong><\/td>\nCentrifuge the vial prior to opening.<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-80\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nDry Ice<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not to be used in humans<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

SUMO\u86cb\u767d\u91761\uff08His\uff09\u9175\u6bcd\u91cd\u7d44\u86cb\u767d| \u8ca8\u865f7868-500 EZCut\u2122 SUMO Protease 1 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[461],"tags":[1723],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23154"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23154"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23154\/revisions"}],"predecessor-version":[{"id":23155,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23154\/revisions\/23155"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23154"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23154"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23154"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}