{"id":22573,"date":"2019-12-04T15:52:06","date_gmt":"2019-12-04T07:52:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22573"},"modified":"2022-07-19T17:53:03","modified_gmt":"2022-07-19T09:53:03","slug":"fumarase%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-fumarase-activity-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk596-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=22573","title":{"rendered":"Fumarase\u6d3b\u6027\u5206\u6790\u5957\u7d44 | Fumarase Activity Colorimetric Assay Kit\u8ca8\u865fK596-100"},"content":{"rendered":"

Fumarase\u6d3b\u6027\u5206\u6790\u5957\u7d44 | <\/strong>\u8ca8\u865f<\/strong>K596-100<\/a><\/span><\/p>\n

Fumarase Activity Colorimetric Assay Kit<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u5bcc\u99ac\u9178\u9176\uff08Fumarase\uff09\u5bcc\u99ac\u9178\u9e7d\u6c34\u5408\u9176\uff1aEC 4.2.1.2\uff09\u662f\u4e00\u7a2e\u50ac\u5316\u5bcc\u99ac\u9178\u9e7d\u6c34\u5408(hydration)\u548c\u812b\u6c34(dehydration)\u6210\u860b\u679c\u9178(Malate)\u7684\u53ef\u9006\u53cd\u61c9\u7684\u9176\u3002\u5b83\u6709\u5169\u7a2e\u5f62\u5f0f\uff1a\u7dda\u7c92\u9ad4\u5f62\u5f0f(mitochondrial)\u548c\u80de\u8cea(cytosolic forms)\u5f62\u5f0f\u3002\u5bcc\u99ac\u9178\u9176\uff08Fumarase\uff09\u7684\u7dda\u7c92\u9ad4\u5f62\u5f0f(mitochondrial)\u662f\u6ab8\u6aac\u9178\u5faa\u74b0(citric acid cycle)\u4e2d\u7684\u95dc\u9375\u9176\u4e4b\u4e00\uff0c\u800c\u80de\u8cea\u5f62\u5f0f(cytosolic forms)\u5c0d\u65bc\u6c28\u57fa\u9178\u548c\u5bcc\u99ac\u9178\u916f\u7684\u4ee3\u8b1d\u5f88\u91cd\u8981\u3002\u5728\u4eba\u985e\u4e2d\uff0c\u5bcc\u99ac\u9178\u9176\u7f3a\u4e4f\u6703\u5c0e\u81f4\u56b4\u91cd\u7684\u5065\u5eb7\u554f\u984c\uff0c\u4f8b\u5982\u80ce\u5152\u8166\u7578\u5f62(fetal brain abnormality)\uff0c\u808c\u5f35\u529b\u4f4e\u4e0b(hypotonia)\u548c\u814e\u7d30\u80de\u764c(renal cell carcinoma)\u3002\u56e0\u6b64\uff0c\u6e96\u78ba\u6e2c\u91cf\u5bcc\u99ac\u9178\u9176\u6d3b\u6027\u5c0d\u65bc\u9810\u9632\uff0c\u8a3a\u65b7\u5bcc\u99ac\u9178\u9176\u7f3a\u4e4f\u75c7\u548c\u6a5f\u5236\u7814\u7a76\u5f88\u91cd\u8981\u3002 Biovision\u7684Fumarase\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u7c21\u4fbf\u7684\u65b9\u6cd5\u4f86\u76e3\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684fumarase\u6d3b\u6027\u3002\u5728\u8a72\u8a66\u5291\u76d2\u4e2d\uff0c\u5bcc\u99ac\u9178\u9176\u5c07\u5bcc\u99ac\u9178\u916f\u8f49\u5316\u70ba\u860b\u679c\u9178(Malate)\uff0c\u7136\u5f8c\u8207\u9176\u6df7\u5408\u7269\u53cd\u61c9\u5f62\u6210\u4e2d\u9593\u9ad4\u3002\u4e2d\u9593\u9ad4\u96a8\u5f8c\u5c07\u986f\u5f71\u5291\u9084\u539f\u70ba\u5728450 nm\u8655\u5177\u6709\u5f37\u5438\u6536\u7387\u7684\u6709\u8272\u7522\u54c1\u3002\u8a72\u6e2c\u5b9a\u65b9\u6cd5\u7c21\u55ae\u6613\u884c\uff0c\u4e26\u4e14\u5177\u6709\u9ad8\u901a\u91cf\u7684\u7279\u9ede\uff0c\u4e26\u4e14\u5728\u5404\u7a2e\u6a23\u54c1\u985e\u578b\u4e2d\u5747\u53ef\u6aa2\u6e2c\u5230\u4f4e\u65bc50 U \/ ml\u7684\u5bcc\u99ac\u9178\u9176\u6d3b\u6027\u3002.\u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf\u00a0<\/strong>ELISA<\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/strong><\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK596-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (OD 450 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of fumarase activity in various tissues\/cells.
\nAnalysis of citric acid cycle, reductive carboxylation cycle (CO2 fixation) and renal cell carcinomas.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple & Rapid Protocol
\n\u2022 High-Throughput
\n\u2022 This kit can detect less than 50 U\/ml of fumarase activity in variety of sample types<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Fumarase Assay Buffer
\n\u2022 Fumarase Substrate
\n\u2022 Fumarase Enzyme Mix (Lyophilized)
\n\u2022 Fumarase Developer (Lyophilized)
\n\u2022 NADH Standard (Lyophilized)
\n\u2022 Fumarase Positive Control (Lyophilized)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Fumarase\u6d3b\u6027\u5206\u6790\u5957\u7d44 | \u8ca8\u865fK596-100 Fumarase Activity Colorimet […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1804],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22573"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22573"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22573\/revisions"}],"predecessor-version":[{"id":22574,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22573\/revisions\/22574"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22573"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22573"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22573"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}