{"id":22571,"date":"2019-12-04T15:31:55","date_gmt":"2019-12-04T07:31:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22571"},"modified":"2022-07-19T17:53:10","modified_gmt":"2022-07-19T09:53:10","slug":"fumarate%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-%e8%b2%a8%e8%99%9fk633-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=22571","title":{"rendered":"Fumarate\u5448\u8272\u5206\u6790\u5957\u7d44 | \u8ca8\u865fK633-100"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=22571\">Fumarate\u5448\u8272\u5206\u6790\u5957\u7d44 <strong>| <\/strong><strong>\u8ca8\u865f<\/strong>K633-100<\/a><\/span><\/p>\n<p>Fumarate Colorimetric Assay Kit<\/p>\n<p>\u7522\u54c1\u4ecb\u7d39: Fumarate\uff08HO 2 CCH = CHCO 2 H-\uff09\u662f\u514b\u96f7\u5e03\u5faa\u74b0(Kreb\u2019s cycle)\u4e2d\u7684\u4e00\u7a2e\u4e2d\u9593\u9ad4(intermediate)\uff0c\u7d30\u80de\u7528\u65bc\u7d30\u80de\u4ee3\u8b1d\u98df\u7269\u4ee5\u5f62\u6210ATP\u3002 \u5728\u54fa\u4e73\u52d5\u7269\u809d\u81df(liver)\u4e2d\uff0c\u5bcc\u99ac\u9178\u916f (Fumarate)\u4e5f\u662f\u5c3f\u7d20\u5faa\u74b0(Urea cycle)\u7684\u7522\u7269\uff0c\u5728\u5c3f\u7d20\u5faa\u74b0\u4e2d\uff0c\u5bcc\u99ac\u9178\u916f\u5728cytosol\u4e2d\u7684\u91cb\u653e\u5c0e\u81f4\u5176\u8f49\u5316\u70ba\u860b\u679c\u9178 (malate)\uff0c\u7136\u5f8c\u8f49\u5316\u70ba\u8349\u9170\u4e59\u9178(oxaloacetate)\uff0c\u540c\u6642\u5728cytosol\u4e2d\u751f\u6210NADH\u3002\u4eba\u9ad4\u76ae\u819a\u5728\u967d\u5149\u4e0b\u81ea\u7136\u6703\u7522\u751f\u5bcc\u99ac\u9178(fumaric acid)\u3002 \u5be6\u969b\u4e0a\uff0c\u5bcc\u99ac\u9178\u916f(fumaric acid esters)\u5df2\u7d93\u88ab\u7528\u65bc\u6cbb\u7642\u725b\u76ae\u766c(psoriasis)\uff0c\u9019\u53ef\u80fd\u662f\u7531\u65bc\u76ae\u819a\u4e2d\u5bcc\u99ac\u9178(fumaric acid)\u7684\u7522\u751f\u53d7\u640d\u3002 \u5bcc\u99ac\u9178\u4e5f\u5df2\u7528\u65bc\u98f2\u6599(beverages)\uff0c\u767c\u9175\u7c89(baking powders)\u548c\u7cd6\u679c(candy)\u4e2d\u3002 BioVision\u7684\u5bcc\u99ac\u9178\u916f\u6e2c\u5b9a\u8a66\u5291\u76d2\u70ba\u9748\u654f\u6aa2\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684\u5bcc\u99ac\u9178\u916f\u63d0\u4f9b\u4e86\u65b9\u4fbf\u7684\u5de5\u5177\u3002 \u5bcc\u99ac\u9178\u916f\u9176\u6df7\u5408\u7269\u5c07\u5bcc\u99ac\u9178\u916f\u8b58\u5225\u70ba\u5c0e\u81f4\u6210\u6bd4\u4f8b\u984f\u8272\u986f\u8272\u7684\u7279\u5b9a\u5e95\u7269\u3002 \u56e0\u6b64\uff0c\u53ef\u4ee5\u4f7f\u7528\u6bd4\u8272\u6e2c\u5b9a\u6cd5\uff08\u03bb= 450 nm\uff09\u8f15\u9b06\u78ba\u5b9a\u5bcc\u99ac\u9178\u916f\u7684\u542b\u91cf\u3002 \u6bcf\u5b54\uff0820 \u00b5M\uff09\u53ef\u4ee5\u6aa2\u6e2c\u5230\u4f4e\u81f31 nmol\u7684\u5bcc\u99ac\u9178\u916f\u3002.\u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf<span style=\"color: #ff9900;\"><strong>\u00a0<\/strong><a style=\"color: #ff9900;\" href=\"http:\/\/biopioneer.com.tw\/?p=1120\"><strong>ELISA<\/strong><strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/strong><\/a><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/6\/k633.jpg\" alt=\"\" width=\"473\" height=\"250\" \/><\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K633-100<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Absorbance (450 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Mammalian<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Kit can detect as low as 1 nmol of fumarate per well (20 \u00b5M).<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Simple procedure; takes ~ 40 minutes<br \/>\n\u2022 Fast and convenient<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 Fumarate Assay Buffer<br \/>\n\u2022 Fumarate Enzyme Mix<br \/>\n\u2022 Fumarate Developer<br \/>\n\u2022 Fumarate Standard (0.1 M)<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage Conditions<\/strong><\/td>\n<td>-20\u00b0C<\/td>\n<\/tr>\n<tr>\n<td><strong>Shipping Conditions<\/strong><\/td>\n<td>Gel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fumarate\u5448\u8272\u5206\u6790\u5957\u7d44 | \u8ca8\u865fK633-100 Fumarate Colorimetric Assay [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1805],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22571"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22571"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22571\/revisions"}],"predecessor-version":[{"id":22572,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22571\/revisions\/22572"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22571"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22571"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22571"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}