{"id":22523,"date":"2019-11-21T10:30:20","date_gmt":"2019-11-21T02:30:20","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22523"},"modified":"2025-03-21T14:28:48","modified_gmt":"2025-03-21T06:28:48","slug":"aiz-ar-stable-22rv1-luciferase-reporter-cell-line-%e8%b2%a8%e8%99%9ft4108","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=22523","title":{"rendered":"Human Endometrial Somatic Stem Cells (Stromal Fraction hESP-ICEs) \u8ca8\u865fT4108"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u85e5\u7269\u7be9\u9078\u5e73\u53f0<\/strong> | <\/strong>\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line\u00a0 | \u8ca8\u865fT4108 <\/a>\u00a0<\/span>\u7d30\u80de\u6578\u91cf1×106<\/sup> cells \/ 1.0 ml<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u5b50\u5bae\u5167\u819c\u9ad4\u5e79\u7d30\u80de(specialized somatic stem cell, SSC)\u662f\u6839\u64da\u5074\u9762\u7fa4\u9ad4\uff08Side Population , SP\uff09\u6280\u8853\u5206\u96e2\u7684\uff0c\u8a72\u6280\u8853\u4f9d\u8cf4\u65bc\u7d30\u80de\u6392\u9664\u8207DNA\u7d50\u5408\u7684Hoechst 33342\u67d3\u6599\u7684\u80fd\u529b\u3002\u57fa\u65bc\u4f4eHoechst 333\uff0c\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u5c0d\u7d30\u80de\u9032\u884c\u6a19\u8a18\u548c\u5206\u985e\u3002\u4eba\u5011\u901a\u5e38\u901a\u904e\u4f7f\u7528\u201c\u5074\u9762\u7a2e\u7fa4\u201d\uff08Side Population , SP\uff09\u6280\u8853\u4f86\u9451\u5b9a\u4eba\u5b50\u5bae\u5167\u819c\u4e2d\u7684\u7279\u5b9a\u9ad4\u7d30\u80de\u5e79\u7d30\u80de\uff08specialized somatic stem cell, SSC\uff09\u7a2e\u7fa4\uff0c\u4ee5\u4ecb\u5c0e\u5b50\u5bae\u5167\u819c\u7684\u518d\u751f\u3002\u4eba\u5b50\u5bae\u5167\u819c\u9ad4\u5e79\u7d30\u80de\u7531\u4eba\u5b50\u5bae\u5167\u819c\u5074\u7fa4\u7d30\u80de\u5efa\u7acb\uff0c\u7279\u5225\u662f\u7531\u4e0a\u76ae\u90e8\u5206\uff08ICEp; \u8ca8\u865fT4107\uff09<\/a><\/span>\u6216\u57fa\u8cea\u90e8\u5206\uff08ICEs\uff1b\u8ca8\u865fT4108\uff09<\/a><\/span>\u5efa\u7acb\u3002\u5169\u7a2e\u7d30\u80de\u985e\u578b\u90fd\u5177\u6709\u5728\u9ad4\u5916\u7d93\u6b77\u6210\u9aa8(osteogenic)\u548c\u6210\u8102\u5206\u5316(adipogenic differentiation)\u7684\u80fd\u529b\u3002\u6210\u8102\u8a98\u5c0e (adipogenic induction) \u5f8c\uff0c\u7d30\u80de\u986f\u793a\u8102\u8cea\u5728\u7d30\u80de\u8cea\u4e2d\u84c4\u7a4d\u4e26\u589e\u52a0\u8102\u86cb\u767d\u8102\u80aa\u9176\uff08LPL\uff09\u7684\u8868\u9054\u3002\u5728\u6210\u9aa8\u8a98\u5c0e\u4e0b\uff0c\u9019\u4e9b\u7d30\u80de\u7684\u9aa8\u553e\u6db2\u86cb\u767d\uff08BSP\uff09\u67d3\u8272\u5448\u967d\u6027\uff0c\u4e26\u986f\u793aosterix\u7684\u8868\u9054\u589e\u52a0\u3002\u6b64\u5916\uff0c\u7576\u5c07\u5169\u7a2e\u7d30\u80de\u985e\u578b\u79fb\u690d\u5230NOD-SCID\u5c0f\u9f20\u7684\u814e\u56ca\u4e0b\u65b9\u6642\uff0c\u5b83\u5011\u90fd\u80fd\u5920\u5728\u9ad4\u5167\u91cd\u5efa\u5168\u90e8\u5b50\u5bae\u5167\u819c\u6a23\u7d44\u7e54\u3002\u4eba\u5b50\u5bae\u5167\u819c\u9ad4\u5e79\u7d30\u80de\u53ef\u4f5c\u70ba\u6e2c\u8a66\u5b50\u5bae\u5167\u819c\u751f\u7406\u5b78\u548c\u75c5\u7406\u5b78\u76f8\u95dc\u9776\u6a19\u7684\u53ef\u9760\u6a21\u578b\u3002<\/p>\n

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 <\/p>\n\n\n\n
Organism<\/strong><\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Homo sapiens<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nHuman endometrium from healthy women<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nFibroblast-like<\/td>\n<\/tr>\n
Population Doubling<\/strong><\/td>\nProliferation ceases after about 20 weeks of culture.<\/td>\n<\/tr>\n
Disease<\/strong><\/td>\nNormal<\/td>\n<\/tr>\n
Isolation<\/strong><\/td>\nEndometrial Somatic Stem Cells are isolated based on the Side Population (SP) technique- a method that replies on the capability of cells to exclude the DNA binding Hoechst 33342 dye. Cells are labeled and sorted by flow cytometry based on low Hoechst 333.<\/td>\n<\/tr>\n
Description<\/strong><\/td>\n\n

A specialized somatic stem cell (SSC) population in the human endometrium, often identified by using the Side Population (SP) technique, is thought to mediate the endometrial regeneration. The Human Endometrial Somatic Stem Cells are established from human endometrial side population cells, specifically from the Epithelial fraction (ICEp; Cat. No. T4107) or the Stromal fraction (ICEs; Cat. No. T4108).<\/p>\n

Both cell types retain the ability to undergo osteogenic and adipogenic differentiation\u00a0in vitro\u00a0<\/em>. After adipogenic induction, the cells show lipid accumulation in the cytoplasm and increased expression of lipoprotein lipase (LPL). When under osteogenic induction, the cells stain positive for bonesialoprotein (BSP) and show increased expression of osterix. Furthermore, both cell types are capable of reconstructing total endometrial-like tissue\u00a0in vivo<\/em>\u00a0when transplanted beneath the renal capsule of NOD-SCID mice. The Human Endometrial Somatic Stem Cells serve as a reliable model to test relevant targets for endometrial physiology and pathology.<\/td>\n<\/tr>\n

Materials from<\/strong><\/td>\nIVI Foundation (C\/ Catedr\u00e1tico Agust\u00edn Escardino n\u00ba 9, PARC CIENTIFIC UNIVERSITAT DE VALENCIA Edificio 3, CUE. 2\u00aa Planta. Despacho 2.02 46980 Paterna (Valencia) Tel. +34 96 390 33 05\u00b7 Fax +34 96 390 25 22 )<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\n1) Purity and origin of cells are confirmed by expression of CD9 (epithelial marker) or vimentin (stromal marker) and CD90, CD73 and CD105 (mesenchymal attributes); 2) Undifferentiated status of the cells are confirmed by expression of OCT4, NANOG, GDF3.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5b50\u5bae\u5167\u819c\u9ad4\u5e79\u7d30\u80de(specialized somatic stem cell, SSC)\u7d30\u80de\u682a |\u5b50\u5bae\u5167\u819c\u9ad4\u5e79\u7d30\u80de(specialized somatic stem cell, SSC)\u662f\u6839\u64da\u5074\u9762\u7fa4\u9ad4\uff08Side Population , SP\uff09\u6280\u8853\u5206\u96e2\u7684\uff0c\u8a72\u6280\u8853\u4f9d\u8cf4\u65bc\u7d30\u80de\u6392\u9664\u8207DNA\u7d50\u5408\u7684Hoechst 33342\u67d3\u6599\u7684\u80fd\u529b\u3002\u57fa\u65bc\u4f4eHoechst 333\uff0c\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u5c0d\u7d30\u80de\u9032\u884c\u6a19\u8a18\u548c\u5206\u985e\u3002 \u5668\u5b98\u4f86\u6e90: \u5065\u5eb7\u5973\u6027\u7684\u5b50\u5bae\u5167\u819cHuman endometrium from healthy women | \u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a><\/span>)\uff0c\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a><\/span>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowIV (<\/u>\u8ca8\u865f<\/u>\u00a0<\/u>TM004<\/u>)<\/u><\/span>\u3002\u70ba\u4e86\u88fd\u5099\u5b8c\u6574\u7684\u751f\u9577\u57f9\u990a\u57fa\uff0c\u5c07\u4e0b\u5217\u7d44\u5206\u6dfb\u52a0\u5230\u57fa\u790e\u57f9\u990a\u57fa\u4e2d\uff1a\u80ce\u725b\u8840\u6e05\uff08TM999\uff09\u81f3\u6700\u7d42\u6fc3\u5ea610\uff05\uff0c2 mM L-glutamine (G275<\/a><\/span>)\uff0c\u548cPenicillin\/Streptomycin\u9752\u9ef4\u7d20\/\u93c8\u9ef4\u7d20\u6eb6\u6db2\uff08G255\uff09\u81f3\u6700\u7d42\u6fc3\u5ea6\u70ba1\uff05\u3002\u4e8c\u6c27\u5316\u78b3\uff08CO2\uff09\uff1a5\uff05\uff0c\u6eab\u5ea6\uff1a37.0\u2103\u3002<\/p>\n

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\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

Abmgood<\/strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

Decomplement<\/strong>\u52a0\u71b1\u975e\u6d3b\u5316<\/strong>FBS<\/strong>\uff1a<\/strong><\/p>\n

1.\u8b93FBS\u74f6\u57284\u00b0C\u4e0b\u5b8c\u5168\u89e3\u51cd\u904e\u591c\u3002<\/p>\n

2.\u5c07FBS\u74f6\u7f6e\u65bc56\u00b0C\u7684\u6c34\u6d74\u4e2d30\u5206\u9418\u3002<\/p>\n

Decomplemented FBS\u53ef\u4ee5\u5728-20\u00b0C\u4e0b\u9577\u671f\u4fdd\u5b58\u3002\u907f\u514d\u983b\u7e41\u7684\u51cd\u878d\u5faa\u74b0\u3002\u6bcf2-3\u5929\u66f4\u63db\u4e00\u6b21\u5b8c\u6574\u7684\u5a92\u9ad4\u3002\u4e0d\u8981\u8b93\u4ecb\u8cea\u984f\u8272\u8b8a\u70ba\u9ec3\u8272\u3002<\/p>\n

Thawing<\/strong>\u7d30\u80de\u89e3\u51cd\u65b9\u6cd5\uff1a<\/strong>(<\/strong>\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f<\/strong>)<\/strong><\/p>\n

1.\u572837\u2103\u6c34\u6d74\u4e2d\u89e3\u51cd\u5c0f\u74f6\uff0c\u76f4\u5230\u53ea\u6709\u4e00\u5c0f\u584a\u51b0\u584a\u3002<\/p>\n

2.\u5c07\u7d30\u80de\u8f49\u79fb\u5230\u542b\u67095ml pre-warmed complete media \u4e4b15ml\u7ba1\u4e2d\u3002<\/p>\n

3.\u5c07\u7d30\u80de\u4ee5290xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

4.\u5c0f\u5fc3\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u4e0d\u8981\u6253\u64fe\u7d30\u80de\u6c89\u6fb1\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u57281ml\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

5.\u5c07\u7d30\u80de\u57f9\u990a\u5728150,000-200,000\u500b\u7d30\u80de\/ cm2\u3002<\/p>\n

Subculture<\/strong>\u7d30\u80de\u7e7c\u4ee3\u65b9\u6cd5<\/strong>:<\/strong>\u00a0(<\/strong>\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f<\/strong>)<\/strong><\/p>\n

1.\u7576\u7d30\u80de70-90\uff05 confluency\u6642\uff0c\u5efa\u8b70\u7e7c\u4ee3\u57f9\u990a\u3002<\/p>\n

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  1. Aspirate old media\u3002\u61f8\u6d6e\u6db2\u4e2d\u7684\u4e00\u4e9b\u7d30\u80de\u4ecd\u7136\u662f\u6d3b\u7d30\u80de\u3002\u6216\u8005\uff0c\u4e5f\u53ef\u4ee5\u6536\u96c6\u542b\u6709\u61f8\u6d6e\u7d30\u80de\u7684\u4e0a\u6e05\u6db2\u4e26\u96e2\u5fc3\uff08Skip to Step 6 in protocol\uff09\u3002<\/li>\n<\/ol>\n

    3.\u52a0\u51651\uff1a1\u6bd4\u4f8b\u76841X\u7121\u83ccPBS\u548c0.25\uff05\u80f0\u86cb\u767d\u9176-EDTA\uff08TM051\uff09\u3002<\/p>\n

    4.\u5728\u5ba4\u6eab\u4e0b\u5b75\u80b2\u7d30\u80de3-5\u5206\u9418\u4e26\u652a\u62cc\u57f9\u990a\u5bb9\u5668\u76f4\u81f390\uff05\u7684\u7d30\u80de\u812b\u843d\u3002<\/p>\n

      \n
    1. \u4e2d\u548c\u80f0\u86cb\u767d\u9176:\u6dfb\u52a0\u7b49\u91cf\u4e4btrypsin + PBS\u3002<\/li>\n<\/ol>\n

      6.\u6536\u96c6\u7d30\u80de\u4e26\u4ee5290xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

      7.\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u5728\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

      8.\u5efa\u8b70split ratio\u6bd4\u4e0d\u8d85\u904e1\uff1a3\u3002<\/p>\n

      Freezing<\/strong>\u7d30\u80de\u51cd\u7d50\uff1a<\/strong>(<\/strong>\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f<\/strong>)<\/strong><\/p>\n

      Complete growth medium with decomplemented FBS to a final concentration of 50% from PAN Biotech (P40-37500) or from Nucleus Biologics (FBS1824-001) and 5% DMSO.<\/p>\n

      \u5132\u5b58\u6eab\u5ea6\uff1a\u6db2\u6c2e\u6c23\u76f8\u3002\u91cd\u8981\u63d0\u793a\uff1a\u6b64\u7d30\u80de\u4fc2\u5c0dFBS\u6279\u6b21\u975e\u5e38\u654f\u611f\u3002\u6211\u5011\u5f37\u70c8\u5efa\u8b70\u6700\u7d42\u7528\u6236\u4f7f\u7528\u5f9ePAN biotech\u6216Nucleus Biologics \u4e4bFBS\u3002<\/p>\n

      \u6162\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217\u7522\u54c1Lentivirus<\/u>\u6162\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>| Lentivirus <\/u>\u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>|\u00a0Lentivirus<\/u>\u75c5\u6bd2<\/u>RT-QPCR<\/u>\u5b9a\u91cf<\/u>(\u5b9a\u91cf)<\/a>\u00a0<\/span><\/p>\n

       <\/p>\n","protected":false},"excerpt":{"rendered":"

      Abmgood\u85e5\u7269\u7be9\u9078\u5e73\u53f0 | \u7d30\u80de\u7cfb\u5217\u5546\u54c1 (immortalized cell) AIZ-AR Stabl […]<\/p>\n","protected":false},"author":3,"featured_media":22524,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[494],"tags":[1814],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22523"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22523"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22523\/revisions"}],"predecessor-version":[{"id":50800,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22523\/revisions\/50800"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/22524"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22523"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22523"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22523"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}