{"id":22365,"date":"2019-10-22T11:48:54","date_gmt":"2019-10-22T03:48:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22365"},"modified":"2022-07-20T09:33:24","modified_gmt":"2022-07-20T01:33:24","slug":"pca3-orf-vector-human-porf-%e8%b2%a8%e8%99%9forf027448-1-0-%c2%b5g-dna","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=22365","title":{"rendered":"PCA3 ORF Vector (Human) (pORF) | \u8ca8\u865fORF027448 (1.0 \u00b5g DNA)"},"content":{"rendered":"

PCA3 ORF Vector (Human) (pORF) | <\/strong>\u8ca8\u865f<\/strong>ORF027448 (1.0 \u00b5g DNA)<\/a><\/span><\/p>\n

Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u5e8f\u5217\u9a57\u8b49\u958b\u653e\u95b1\u8b80\u6846\uff08ORF\uff09\u5eab\uff0c\u5305\u62ec\u4eba(human)\uff0c\u5c0f\u9f20(mouse)\u548c\u5927\u9f20(rat)\uff0c\u53efinsertion\u81f3\u60a8\u9078\u64c7\u7684\u86cb\u767d\u8cea\u8868\u9054\u7cfb\u7d71(protein expression systems.)\u3002\u53bb\u96645’UTR\u548c3’UTR\uff0cORF\u63d2\u5165\u517c\u5bb9\u7684\u8868\u9054\u8f09\u9ad4(compatible expression vector) \u5f8c\u5373\u53ef\u5feb\u901f\u53ef\u9760\u5730\u8868\u9054\u86cb\u767d\u8cea\u3002\u4e5f\u53ef\u7528\u4f5cPCR\u64f4\u589e (PCR amplification experiments) \u5be6\u9a57\u4e2d\u967d\u6027\u5c0d\u7167(positive control)\u7684\u6a21\u677f(template)\u3002Abmgood\u63d0\u4f9b\u7684ORF\u5305\u542b\u4e00\u500b\u7a81\u8b8a\u7684\u7d42\u6b62\u5bc6\u78bc\u5b50(mutated stop codon)\uff0c\u5982\u679c\u53d7\u9ad4\u8f09\u9ad4\u4e2d\u5b58\u5728C\u672b\u7aef\u6a19\u7c64\u6216\u5831\u544a\u57fa\u56e0\uff0c\u5247\u5141\u8a31\u5176\u647b\u5165\u3002 abm\u7684ORF\u4e5f\u53ef\u4ee5\u4ee5\u5373\u7528\u578b\u86cb\u767d\u8868\u9054\u8f09\u9ad4(protein expression vectors<\/a><\/span>)\uff0c\u6162\u75c5\u6bd2\u8f09\u9ad4(lentiviral vectors<\/a><\/span>)\u6216\u5305\u88dd\u7684\u6162\u75c5\u6bd2(\u00a0lentivirus<\/a><\/span>)\u548c\u817a\u75c5\u6bd2(\u00a0adenovirus<\/span>\u00a0<\/a>)\u4e4b\u67b6\u69cb\u597d\u4e4b\u8f09\u9ad4\u6216\u75c5\u6bd2\u6db2\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n

PCA3 <\/strong>ORF\u8f09\u9ad4\u4ecb\u7d39: Accession Number<\/strong> NR_015342<\/a>\u00a0\u67b6\u69cb
\npORF<\/a>\u3002\u00a0PCA3 ORF\u8f09\u9ad4\u5728AflII\u548cEcoRV\u5207\u5272\u4f4d\u9ede\u4e4b\u9593\u4fdd\u7559\u4e86\u8a72PCA3\u00a0\u57fa\u56e0\uff08\u9650\u5236\u6027\u9176\u5207\u65b9\u6cd5\uff09\u3002Inserts\u4e0d\u5305\u542b\u4efb\u4f55\u5167\u90e8AflII\u548cEcoRV cut site\uff0c\u53ef\u4ee5AflII\u548cEcoRV\u5207\u9664\u3002\u5728\u63d2\u5165\u7247\u6bb5\u5305\u542b\u9019\u4e9b\u5167\u90e8\u5207\u5272\u4f4d\u9ede\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5PCR\u64f4\u589e\u5e36\u6709\u512a\u9078\u9650\u5236\u6027\u9176\u5207\u4f4d\u9ede\u7684\u63d2\u5165\u7247\u6bb5\uff0c\u4ee5\u9032\u884csubcloning\u3002<\/p>\n

\"pORF\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Insert<\/strong><\/td>\nPCA3<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nThe PCA3 ORF Vector holds the gene (cloned by a restriction enzyme-independent method) between an AflII and EcoRV cut site. Inserts are flanked by and can be excised using AflII and EcoRV as long as inserts do not contain any internal AflII and EcoRV sites. In the case that the insert contains these internal cut sites, the end user can PCR amplify the insert with preferred restriction sites for subcloning.<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nNR_015342<\/a><\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npORF<\/a><\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n2596bp<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\n3735bp<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Selection Marker<\/strong><\/td>\nBacterial: Spectinomycin<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Stability<\/strong><\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n
Caution<\/strong><\/td>\nPCA3 ORF Vector (Human) (pORF) is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5176\u5b83\u5546\u54c1<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/span><\/p>\n

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PCA3 ORF Vector (Human) (pORF) | \u8ca8\u865fORF027448 (1.0 \u00b5g DN […]<\/p>\n","protected":false},"author":3,"featured_media":14324,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[1862],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22365"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22365"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22365\/revisions"}],"predecessor-version":[{"id":22366,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22365\/revisions\/22366"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14324"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22365"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22365"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22365"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}