{"id":22340,"date":"2019-10-18T16:59:29","date_gmt":"2019-10-18T08:59:29","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22340"},"modified":"2022-07-20T10:38:36","modified_gmt":"2022-07-20T02:38:36","slug":"channelrhodopsin-2-eyfp-lentiviral-vector-c-reinhardtii-%e8%b2%a8%e8%99%9f-lv010100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=22340","title":{"rendered":"channelrhodopsin-2-EYFP Lentiviral Vector (C.reinhardtii) | \u8ca8\u865f LV010100"},"content":{"rendered":"

channelrhodopsin-2-EYFP Lentiviral Vector (C.reinhardtii) | \u8ca8\u865f\u00a0 LV010100<\/a><\/span><\/h3>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> channelrhodopsin-2 fused with EYFP \u67b6\u69cb\u65bcpLenti-SynapsinI<\/a>\u8868\u73fe\u8f09\u9ad4\u4e0a\u3002H134R mutation in ChR2, fused to EYFP; Driven by human synapsin1 promoter\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Insert<\/strong><\/td>\nchannelrhodopsin-2 fused with EYFP<\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-SynapsinI<\/a><\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n9000bp<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\n1662 bp<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nC.reinhardtii<\/td>\n<\/tr>\n
Reporter\/Tag<\/strong><\/td>\nNo Tags<\/td>\n<\/tr>\n
Promoter<\/strong><\/td>\nhuman SynapsinI<\/td>\n<\/tr>\n
Selection Marker<\/strong><\/td>\nAmp<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nPlasmid<\/td>\n<\/tr>\n
Viral Packaging<\/strong><\/td>\nabm<\/strong>\u00a0recommends using Cat#\u00a0G074, Lentifectin\u2122<\/a>\u00a0in conjunction with one of the following packaging mixes. The suggested packaging mix for this vector is Cat#\u00a0LV003, 2nd Generation Packaging Mix<\/a>. Alternatively, you can also use Cat#\u00a0LV053, 3rd Generation Packaging Mix<\/a><\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Stability<\/strong><\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

channelrhodopsin-2-EYFP Lentiviral Vector (C.reinhardti […]<\/p>\n","protected":false},"author":3,"featured_media":22341,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1867],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22340"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22340"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22340\/revisions"}],"predecessor-version":[{"id":22344,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22340\/revisions\/22344"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/22341"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22340"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22340"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22340"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}