{"id":22314,"date":"2019-10-14T13:38:39","date_gmt":"2019-10-14T05:38:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22314"},"modified":"2025-10-17T18:50:07","modified_gmt":"2025-10-17T10:50:07","slug":"%e7%a3%ba%e8%83%ba%e5%a4%9a%e5%b7%b4%e9%85%9ab%ef%bc%88sulforhodamine-b-srb%ef%bc%89%e7%b4%b0%e8%83%9e%e7%b4%b0%e8%83%9e%e6%af%92%e6%80%a7%e6%b8%ac%e5%ae%9a-%e8%b2%a8%e8%99%9fk943","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=22314","title":{"rendered":"\u78fa\u80fa\u591a\u5df4\u915aB\uff08Sulforhodamine B ,SRB\uff09\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a| \u8ca8\u865fK943 (ab235935)"},"content":{"rendered":"

\u78fa\u80fa\u591a\u5df4\u915aB\uff08Sulforhodamine B ,SRB\uff09\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u6cd5 | <\/span><\/strong>\u8ca8\u865f<\/strong>K943\u00a0 (ab235935)<\/span><\/a><\/span><\/p>\n

Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric)<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u78fa\u80fa\u591a\u5df4\u915aB\uff08Sulforhodamine B ,SRB\uff09\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u6cd5\u662f\u6aa2\u6e2c\u7d30\u80de\u6d3b\u529b(cell viability)\u6216\u85e5\u7269\u7d30\u80de\u6bd2\u6027(drug cytotoxicity)\u6700\u5ee3\u6cdb\u7684\u65b9\u6cd5\u4e4b\u4e00\u3002\u8a72\u6e2c\u5b9a\u6cd5\u4f9d\u8cf4SRB\u7d50\u5408\u7d30\u80de\u86cb\u767d\u6210\u5206\u4e26\u6e2c\u91cf\u7e3d\u751f\u7269\u91cf(total biomass)\u7684\u80fd\u529b\u3002 SRB\u662f\u4e00\u7a2e\u4eae\u7c89aminoxanthene\u67d3\u6599(bright-pink aminoxanthene dye)\uff0c\u53ef\u4ee5\u5728\u5f31\u9178\u6027\u689d\u4ef6\u4e0b\u8207\u86cb\u767d\u8cea\u7684\u9e7c\u6027\u6c28\u57fa\u9178\u6b98\u57fa(basic amino acid residues)\u5f62\u6210\u975c\u96fb\u8907\u5408\u7269(electrostatic complex)\uff0c\u4f46\u5728\u9e7c\u6027\u689d\u4ef6(basic conditions)\u4e0b\u53ef\u4ee5\u89e3\u96e2\u3002\u5b83\u5df2\u88ab\u5ee3\u6cdb\u7528\u65bc\u91dd\u5c0d\u4e0d\u540c\u985e\u578b\u7684\u764c\u7d30\u80de(cancerous)\u548c\u975e\u764c\u7d30\u80de\u682a(non-cancerous cell lines)\u7684\u85e5\u7269\u6bd2\u6027\u7be9\u9078\u3002\u53e6\u5916\uff0c\u8a72\u6e2c\u5b9a\u6cd5\u4e0d\u4f9d\u8cf4\u65bc\u7d30\u80de\u4ee3\u8b1d\u6d3b\u6027\uff0c\u56e0\u6b64\u61c9\u986f\u793a\u8f03\u5c11\u7684\u53d7\u8a66\u5316\u5408\u7269(testing compounds)\u5e72\u64fe\u3002\u7531\u65bcSRB\u7684\u7d50\u5408\u662f\u5316\u5b78\u8a08\u91cf\u7684\uff0c\u56e0\u6b64\u6d17\u6ecc\u5f8c\u5f9e\u67d3\u8272\u7d30\u80de\u91cb\u653e\u7684\u647b\u5165\u67d3\u6599\u8207\u7d30\u80de\u751f\u7269\u91cf\u6210\u6b63\u6bd4\uff0c\u53ef\u4ee5\u5728565 nm\u8655\u6e2c\u91cf\u3002 BioVision\u7684SRB\u7d30\u80de\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u7c21\u55ae\uff0c\u6e96\u78ba\uff0c\u53ef\u91cd\u8907\u4e14\u9748\u654f\u3002\u8a72\u8a66\u5291\u76d2\u70ba\u9ad4\u5916\u7d30\u80de\u6bd2\u6027\u7814\u7a76\u4ee5\u53ca\u9ad8\u901a\u91cf\u85e5\u7269\u7be9\u9078\u63d0\u4f9b\u4e86\u51fa\u8272\u800c\u6709\u6548\u7684\u65b9\u6cd5\uff0c\u6bcf\u5b54\u53ef\u6aa2\u6e2c\u52305,000-50,000\u500b\u7d30\u80de\u3002<\/p>\n

\"\"\"Functional<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK943-1000\u00a0 (ab235935)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n1000 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nDetection method- Absorbance (565 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nSpecies reactivity- Mammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThis assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than 2 hours
\n\u2022 Fast, sensitive and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Fixation Solution
\n\u2022 20X Washing Solution
\n\u2022 10X Solubilization Solution
\n\u2022 SRB Dye Solution
\n\u2022 20 mM Doxorubicin<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u78fa\u80fa\u591a\u5df4\u915aB\uff08Sulforhodamine B ,SRB\uff09\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u6cd5 | \u8ca8\u865fK943\u00a0 (ab23593 […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,3],"tags":[1873,1872],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22314"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22314"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22314\/revisions"}],"predecessor-version":[{"id":53112,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22314\/revisions\/53112"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22314"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22314"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22314"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}