{"id":22068,"date":"2019-09-24T17:09:38","date_gmt":"2019-09-24T09:09:38","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22068"},"modified":"2023-07-21T11:32:35","modified_gmt":"2023-07-21T03:32:35","slug":"ecl-substrate-western-blot-hrp%e5%86%b7%e5%85%89%e8%a9%a6%e5%8a%91t-pro%e8%b2%a8%e8%99%9f-jt96-k004m","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=22068","title":{"rendered":"ECL substrate | Western blot HRP\u51b7\u5149\u8a66\u5291T-PRO\u8ca8\u865f JT96-K004M"},"content":{"rendered":"

T-Pro LumiLond Plus Chemiluminescent Substrate Kit\u00a0 JT96-K004S 100ml*2\u00a0 JT96-K004M 250ml*2\u00a0 \u00a0\u00a0<\/strong><\/p>\n

T-Pro LumiLong Plus Chemiluminescent Substrate Kit (20)<\/strong><\/a><\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/p>\n

T-Pro LumiLong Plus Chemiluminescent Substrate Kit\u70ba\u5229\u7528\u5316\u5b78\u5448\u8272\u6cd5\u5075\u6e2cHorseradish peroxide (HRP)\u6d3b\u6027\u7684substrate\u8a66\u5291\u5957\u7d44\uff0c\u53ef\u9069\u7528\u65bc\u8a31\u591a\u5206\u6790\u8a66\u9a57\uff0c\u4f8b\u5982\uff1a\u897f\u65b9\u58a8\u9ede\u6cd5\u3001\u5357\u65b9\u58a8\u9ede\u6cd5\u8207\u5317\u65b9\u58a8\u9ede\u6cd5\u3002HRP\u53ef\u7d93\u7531H2<\/sub>O2<\/sub>\u50ac\u5316\u74b0\u72c0diacylhydrazides\u7684\u5316\u5b78\u767c\u5149\u6c27\u5316\uff0c\u4f8b\u5982\uff1aluminol\u3002T-Pro LumiLong Plus Chemiluminescent Substrate Kit\u53ef\u4ee5\u52a0\u5f37luminol-dependent\u4e4b\u5316\u5b78\u767c\u5149\u5448\u8272\uff0c\u4e26\u53ef\u5ee3\u6cdb\u4f7f\u7528\u5728\u5229\u7528HRP-conjugated\u4e4b\u6297\u9ad4\u8fa8\u8b58\u7d50\u5408\u4e4b\u6297\u539f\u6216\u662f\u5229\u7528streptavidin<\/p>\n

\u8a0a\u865f\u6301\u4e45\u5ea6\u597d\u00a0– 12 hours
\n\u5efa\u8b70\u6297\u9ad4\u7a00\u91cb\u500d\u7387 – \u521d\u7d1a\u6297\u9ad41\/1,000 \u2013 1\/5,000\uff1b\u4e8c\u7d1a\u6297\u9ad4\u7684\u7a00\u91cb\u500d\u73871\/20,000 \u2013 1\/100,000
\n\u6700\u4f4e\u5075\u6e2c\u9650\u5236\u00a0– Low-Picomoles: 10-10<\/sup>\uff1bHigh-Zeptogram: 10-13<\/sup><\/p>\n

\u5be6\u9a57\u8ad6\u8b49<\/strong><\/p>\n

None<\/p>\n

\u4fdd\u5b58\u689d\u4ef6<\/strong><\/p>\n

\u4fdd\u5b58\u65bc4\u2103<\/p>\n

\u8a02\u8cfc\u8cc7\u8a0a<\/strong><\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
JB02-B006S<\/td>\nT-Pro EZ Gel Solution 6%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B006M<\/td>\nT-Pro EZ Gel Solution 6%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB02-B008S<\/td>\nT-Pro EZ Gel Solution 8%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B008M<\/td>\nT-Pro EZ Gel Solution 8%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB02-B010S<\/td>\nT-Pro EZ Gel Solution 10%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B010M<\/td>\nT-Pro EZ Gel Solution 10%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB02-B012S<\/td>\nT-Pro EZ Gel Solution 12%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B012M<\/td>\nT-Pro EZ Gel Solution 12%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB02-B015S<\/td>\nT-Pro EZ Gel Solution 15%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B015M<\/td>\nT-Pro EZ Gel Solution 15%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB03-C001<\/td>\nT-Pro Separating or Resolving Buffer<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB03-C002<\/td>\nT-Pro Stacking Buffer<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB04-D001<\/td>\nT-Pro BCA Protein Assay kit<\/td>\n500assay\/KIT<\/td>\n<\/tr>\n
JB04-D001A<\/td>\nT-Pro BCA Protein Assay Reagent A<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB04-D001B<\/td>\nT-Pro BCA Protein Assay Reagent B<\/td>\n12ml\/BT<\/td>\n<\/tr>\n
JB04-D001C<\/td>\nAlbumin Standard (2.0 mg\/ml)<\/td>\n5*1ml\/kit<\/td>\n<\/tr>\n
JB04-D002<\/td>\nT-Pro Bradford\u00a0 Protein Assay kit (1X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB06-F002<\/td>\nT-Pro Laemmli (SDS sample) Reagent (reducing 4X)<\/td>\n10ml\/BT<\/td>\n<\/tr>\n
JB06-F003<\/td>\nT-Pro Laemmli (SDS sample) Reagent (non-reducing 4X)<\/td>\n10ml\/BT<\/td>\n<\/tr>\n
JB07-G001<\/td>\nT-Pro Tris-Glycine-SDS running buffer (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB07-G002<\/td>\nT-Pro Tris-Glycine-Native running buffer (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB07-G003<\/td>\nT-Pro MOPS\/SDS running buffer (20X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB08-H001<\/td>\nT-Pro Transfer Blotting buffer (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB08-H002<\/td>\nT-Pro Semi Dry Transfer Blotting buffer (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB09-I001<\/td>\nT-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB09-I002<\/td>\nT-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB09-I003<\/td>\nT-Pro Washing buffer in PBS and Tween-20 (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB09-I004<\/td>\nT-Pro Washing buffer in TBS and Tween-20 (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB11-K002<\/td>\nT-Pro Western Blot Stripping Reagent<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JT90-R001S<\/td>\nT-Pro Aqua EZ Clean (S)<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JT90-R001M<\/td>\nT-Pro Aqua EZ Clean (M)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JT90-R002<\/td>\nT-Pro MycoClean spray<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JT90-R005S<\/td>\nT-Pro EZ stain Gel solution<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JT90-R005M<\/td>\nT-Pro EZ stain Gel solution<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JK92-W001<\/td>\nT-Pro Fast Blocking Buffer (in PBS)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JK92-W002<\/td>\nT-Pro Fast Blocking Buffer (in TBS)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JK92-W003<\/td>\nT-Pro Protein Free Blocking Buffer (in PBS)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JK92-W004<\/td>\nT-Pro Protein Free Blocking Buffer (in TBS)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JT96-K002S<\/td>\nT-Pro LumiFast Plus ECL Kit<\/td>\n100ml*2\/Kit<\/td>\n<\/tr>\n
JT96-K002M<\/td>\nT-Pro LumiFast Plus ECL Kit<\/td>\n250ml*2\/Kit<\/td>\n<\/tr>\n
JT96-K004S<\/td>\nT-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)<\/td>\n100ml*2\/Kit<\/td>\n<\/tr>\n
JT96-K004M<\/td>\nT-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)<\/td>\n250ml*2\/Kit<\/td>\n<\/tr>\n
JT97-N001M<\/td>\nT-Pro Nonliposomal Transfection Reagent I (NTR I)<\/td>\n1.0ml\/vial<\/td>\n<\/tr>\n
JT97-N002M<\/td>\nT-Pro Nonliposomal Transfection Reagent II (NTR II)<\/td>\n1.0ml\/vial<\/td>\n<\/tr>\n
JT97-N006M<\/td>\nT-Pro Nonliposomal Transfection Reagent III (NTR III)<\/td>\n1.0ml\/vial<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

T-Pro LumiLond Plus Chemiluminescent Substrate Kit\u00a0 JT9 […]<\/p>\n","protected":false},"author":3,"featured_media":783,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[135],"tags":[1898],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22068"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22068"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22068\/revisions"}],"predecessor-version":[{"id":44233,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22068\/revisions\/44233"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/783"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22068"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22068"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22068"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}