{"id":22060,"date":"2019-09-24T17:01:30","date_gmt":"2019-09-24T09:01:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22060"},"modified":"2022-07-20T10:43:52","modified_gmt":"2022-07-20T02:43:52","slug":"ecl-substrate-western-blot-hrp%e5%86%b7%e5%85%89%e8%a9%a6%e5%8a%91t-pro%e8%b2%a8%e8%99%9f-jt96-k002s","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=22060","title":{"rendered":"ECL substrate | western blot HRP\u51b7\u5149\u8a66\u5291T-PRO\u8ca8\u865f JT96-K002S"},"content":{"rendered":"

T-Pro LumiFast Plus Chemiluminescent Substrate Kit\u00a0 JT96-K002S 100ml*2\u00a0 JT96-K002M\u00a0250ml*2\u00a0 \u00a0\u00a0<\/strong><\/p>\n

\u7522\u54c1\u8aaa\u660e<\/strong><\/p>\n

T-Pro LumiFast Plus Chemiluminescent Substrate Kit\u70ba\u5229\u7528\u5316\u5b78\u5448\u8272\u6cd5\u5075\u6e2cHorseradish peroxide (HRP)\u6d3b\u6027\u7684substrate\u8a66\u5291\u5957\u7d44\uff0c\u53ef\u9069\u7528\u65bc\u8a31\u591a\u5206\u6790\u8a66\u9a57\uff0c\u4f8b\u5982\uff1a\u897f\u65b9\u58a8\u9ede\u6cd5\u3001\u5357\u65b9\u58a8\u9ede\u6cd5\u8207\u5317\u65b9\u58a8\u9ede\u6cd5\u3002HRP\u53ef\u7d93\u7531H2<\/sub>O2<\/sub>\u50ac\u5316\u74b0\u72c0diacylhydrazides\u7684\u5316\u5b78\u767c\u5149\u6c27\u5316\uff0c\u4f8b\u5982\uff1aluminol\u3002T-Pro LumiFast Plus Chemiluminescent Substrate Kit\u53ef\u4ee5\u52a0\u5f37luminol-dependent\u4e4b\u5316\u5b78\u767c\u5149\u5448\u8272\uff0c\u4e26\u53ef\u5ee3\u6cdb\u4f7f\u7528\u5728\u5229\u7528HRP-conjugated\u4e4b\u6297\u9ad4\u8fa8\u8b58\u7d50\u5408\u4e4b\u6297\u539f\u6216\u662f\u5229\u7528streptavidin<\/p>\n

\u7522\u54c1\u7279\u8272<\/strong><\/p>\n

\u8a0a\u865f\u6301\u4e45\u5ea6\u597d\u00a0– 6-8 hours
\n\u5efa\u8b70\u6297\u9ad4\u7a00\u91cb\u500d\u7387\u00a0–\u00a0\u521d\u7d1a\u6297\u9ad41\/1,000 \u2013 1\/5,000\uff1b\u4e8c\u7d1a\u6297\u9ad4\u7684\u7a00\u91cb\u500d\u73871\/20,000 \u2013 1\/100,000
\n\u6700\u4f4e\u5075\u6e2c\u9650\u5236\u00a0 – Low: Picomoles: 10-12<\/sup>\uff1bHigh: Femtogram: 10-1<\/sup><\/p>\n

\"\"<\/p>\n

\u5be6\u9a57\u8ad6\u8b49<\/strong><\/p>\n

None<\/p>\n

\u4fdd\u5b58\u689d\u4ef6<\/strong><\/p>\n

\u4fdd\u5b58\u65bc4\u2103<\/p>\n

\u8a02\u8cfc\u8cc7\u8a0a<\/strong><\/p>\n

\"\"<\/p>\n

 <\/p>\n

T-Pro LumiLong Plus Chemiluminescent Substrate Kit (20)<\/strong><\/p>\n

\u7522\u54c1\u8aaa\u660e<\/strong><\/p>\n

T-Pro LumiLong Plus Chemiluminescent Substrate Kit\u70ba\u5229\u7528\u5316\u5b78\u5448\u8272\u6cd5\u5075\u6e2cHorseradish peroxide (HRP)\u6d3b\u6027\u7684substrate\u8a66\u5291\u5957\u7d44\uff0c\u53ef\u9069\u7528\u65bc\u8a31\u591a\u5206\u6790\u8a66\u9a57\uff0c\u4f8b\u5982\uff1a\u897f\u65b9\u58a8\u9ede\u6cd5\u3001\u5357\u65b9\u58a8\u9ede\u6cd5\u8207\u5317\u65b9\u58a8\u9ede\u6cd5\u3002HRP\u53ef\u7d93\u7531H2<\/sub>O2<\/sub>\u50ac\u5316\u74b0\u72c0diacylhydrazides\u7684\u5316\u5b78\u767c\u5149\u6c27\u5316\uff0c\u4f8b\u5982\uff1aluminol\u3002T-Pro LumiLong Plus Chemiluminescent Substrate Kit\u53ef\u4ee5\u52a0\u5f37luminol-dependent\u4e4b\u5316\u5b78\u767c\u5149\u5448\u8272\uff0c\u4e26\u53ef\u5ee3\u6cdb\u4f7f\u7528\u5728\u5229\u7528HRP-conjugated\u4e4b\u6297\u9ad4\u8fa8\u8b58\u7d50\u5408\u4e4b\u6297\u539f\u6216\u662f\u5229\u7528streptavidin<\/p>\n

\u7522\u54c1\u7279\u8272<\/strong><\/p>\n

\u8a0a\u865f\u6301\u4e45\u5ea6\u597d\u00a0– 12 hours
\n\u5efa\u8b70\u6297\u9ad4\u7a00\u91cb\u500d\u7387\u00a0–\u00a0\u521d\u7d1a\u6297\u9ad41\/1,000 \u2013 1\/50,000\uff1b\u4e8c\u7d1a\u6297\u9ad4\u7684\u7a00\u91cb\u500d\u73871\/50,000 \u2013 1\/250,000
\n\u6700\u4f4e\u5075\u6e2c\u9650\u5236\u00a0– Low-Picomoles: 10-14<\/sup>\uff1bHigh-Zeptogram: 10-19<\/sup><\/p>\n

\u5be6\u9a57\u8ad6\u8b49<\/strong><\/p>\n

None<\/p>\n

\u4fdd\u5b58\u689d\u4ef6<\/strong><\/p>\n

\u4fdd\u5b58\u65bc4\u2103<\/p>\n

\u8a02\u8cfc\u8cc7\u8a0a<\/strong><\/p>\n

\"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

T-Pro LumiFast Plus Chemiluminescent Substrate Kit\u00a0 JT9 […]<\/p>\n","protected":false},"author":3,"featured_media":783,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[135],"tags":[1901],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22060"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22060"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22060\/revisions"}],"predecessor-version":[{"id":22063,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22060\/revisions\/22063"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/783"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22060"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22060"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22060"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}