{"id":21937,"date":"2019-09-16T11:39:30","date_gmt":"2019-09-16T03:39:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21937"},"modified":"2022-07-20T10:47:23","modified_gmt":"2022-07-20T02:47:23","slug":"%e5%b7%a6%e6%97%8b%e8%82%89%e9%b9%bc%e6%aa%a2%e6%b8%ac-l-carnitine-assay-kit%e8%b2%a8%e8%99%9fk642-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21937","title":{"rendered":"\u5de6\u65cb\u8089\u9e7c\u6aa2\u6e2c | L-Carnitine Assay Kit\u8ca8\u865fK642-100"},"content":{"rendered":"

\u5de6\u65cb\u8089\u9e7c\u6aa2\u6e2c\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K642-100<\/a><\/span><\/p>\n

L-Carnitine Assay Kit<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u8089\u9e7c(Carnitine)\u6c28\u57fa\u9178\u8cf4\u6c28\u9178(Lysine)\u548c\u86cb\u6c28\u9178(methionine)\u751f\u7269\u5408\u6210\u7684\u5b63\u92a8\u5316\u5408\u7269(quaternary ammonium)\u9700\u8981\u901a\u904e\u8089\u6bd2\u9e7c\/\u9170\u57fa\u8089\u9e7c\u7a7f\u68ad(carnitine\/acylcarnitine shuttle)\u5c07\u8102\u80aa\u9178(fatty acids)\u8f49\u904b\u5230\u7dda\u7c92\u9ad4\u57fa\u8cea(mitochondrial matrix)\u4e2d\uff0c\u5176\u4e2d\u767c\u751f\u03b2-\u6c27\u5316(\u03b2-oxidation)\uff0c\u7522\u751f\u4e59\u9178\u9e7d(acetate)\u4e26\u4e14\u5728TCA\u5faa\u74b0\u4e2d\u5229\u7528\u4e59\u9178\u9e7d(acetate)\u7522\u751f\u80fd\u91cf\u3002 \u5de6\u65cb\u8089\u9e7c(L-Carnitine)\u901a\u5e38\u4f5c\u70ba\u71df\u990a\u88dc\u5145\u5291\u51fa\u552e\u3002 \u8089\u9e7c\u4ee5\u5169\u7a2e\u7acb\u9ad4\u7570\u69cb\u9ad4(stereoisomers)\u5b58\u5728\u3002 \u53ea\u6709\u5de6\u65cb\u8089\u9e7c(L-Carnitine)\u5177\u6709\u751f\u7269\u6d3b\u6027\u3002 BioVision\u7684\u5de6\u65cb\u8089\u9e7c\u6aa2\u6e2c\u8a66\u5291\u76d2(L-Carnitine Assay Kit, k777)\u662f\u6e2c\u91cf\u751f\u7269\u6a23\u54c1\uff08\u5982\u8840\u6e05\uff09\u4e2d\u6e38\u96e2L-\u8089\u9e7c(free L-Carnitine)\u7684\u7c21\u4fbf\u65b9\u6cd5\u3002 \u8a72\u6e2c\u5b9a\u5c07\u4e59\u9170\u57fa(acetyl group)\u5f9eCoA\u8f49\u79fb\u81f3\u8089\u6bd2\u9e7c(carnitine)\uff0c\u4e26\u4e14\u96a8\u5f8c\u6c27\u5316Oxi-Red\u63a2\u91dd\u9032\u4e00\u6b65\u8655\u7406\u5f62\u6210\u7684\u6e38\u96e2CoA\uff0c\u5f97\u5230\u87a2\u5149\uff08Ex \/ Em 535nm 587nm\uff09\u548c\u5438\u5149\u5ea6\uff08570nm\uff09\u3002 \u8840\u6e05L-\u8089\u9e7c\u7684\u6b63\u5e38\u7bc4\u570d\u662f~20-100\u03bcM\u3002 \u87a2\u5149\u6e2c\u5b9a\u6cd5\u7684\u6aa2\u6e2c\u9748\u654f\u5ea6\u70ba~1\u03bcM\uff0c\u6bd4\u8272\u6e2c\u5b9a\u6cd5\u7684\u6aa2\u6e2c\u9748\u654f\u5ea6\u70ba~10\u03bcM\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK642-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (570 nm) or Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe detection sensitivity is ~1 \u00b5M for the fluorometric assay and ~10 \u00b5M for the colorimetric assay.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 30 minutes
\n\u2022 Fast and convenient
\n\u2022 Kit contains all necessary reagents for accurate measurement of L-Carnitine levels<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Carnitine Assay Buffer
\n\u2022 Carnitine Probe (in DMSO)
\n\u2022 Carnitine Converting Enzyme
\n\u2022 Carnitine Substrate Mix
\n\u2022 Carnitine Development Mix
\n\u2022 Carnitine Standard (10 \u00b5mol)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Jamie A. et al., Characterization of\u00a0Pseudomonas aeruginosa<\/em>\u00a0Growth on\u00a0O<\/em>-Acylcarnitines and Identification of a Short-Chain Acylcarnitine Hydrolase.\u00a0Appl. Envir. Microbiol., Jun 2013; 79: 3355 – 3363.<\/a><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u5de6\u65cb\u8089\u9e7c\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK642-100 L-Carnitine Assay Kit \u7522\u54c1\u63cf\u8ff0\uff1a\u8089\u9e7c(Ca […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1930],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21937"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21937"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21937\/revisions"}],"predecessor-version":[{"id":21938,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21937\/revisions\/21938"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21937"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21937"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21937"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}