{"id":21920,"date":"2019-09-11T14:45:16","date_gmt":"2019-09-11T06:45:16","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21920"},"modified":"2022-07-20T10:48:10","modified_gmt":"2022-07-20T02:48:10","slug":"%e8%b0%b7%e6%b0%a8%e9%85%b0%e8%83%ba%e9%85%b6%ef%bc%88gls1%ef%bc%89%e6%8a%91%e5%88%b6%e5%8a%91%e7%af%a9%e9%81%b8%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk479","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21920","title":{"rendered":"\u8c37\u6c28\u9170\u80fa\u9176\uff08GLS1\uff09\u6291\u5236\u5291\u7be9\u9078| Glutaminase (GLS1) Inhibitor Screening Kit \u8ca8\u865fK479"},"content":{"rendered":"

\u8c37\u6c28\u9170\u80fa\u9176\uff08GLS1\uff09\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 | \u8ca8\u865fK479<\/a><\/span><\/p>\n

Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)<\/em>\u00a0<\/em><\/p>\n

\"\"\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u8c37\u6c28\u9170\u80fa\u9176\uff08Glutaminase , EC 3.5.1.2\uff0cGLS\uff09\u662f\u6c34\u89e3\u7522\u751f\u8c37\u6c28\u9170\u80fa(glutamate)\u548c\u6c28(ammonia)\u7684\u8c37\u6c28\u9170\u80fa\u7684\u9176\u3002\u5b83\u5728\u591a\u500b\u5668\u5b98\u4e2d\u5177\u6709\u7d44\u7e54\u7279\u7570\u6027(tissue-specific)\u4f5c\u7528\uff0c\u5176\u4e2d\u4e00\u4e9b\u5305\u62ec\u7528\u65bc\u809d\u81df\u4e2d\u5c3f\u7d20\u5408\u6210(urea synthesis)\u7684\u6c28\u751f\u6210(ammonia generation); \u5728\u814e\u81df\u814e\u9178\u4e2d\u6bd2\u671f\u9593\u901a\u904e\u6c28\u751f\u6210\u7dad\u6301\u9178\u9e7c\u7a69\u614b;\u548c\u8abf\u7bc0\u8166\u4e2d\u7684\u795e\u7d93\u905e\u8cea\u8c37\u6c28\u9178\u3002\u540c\u7a2e\u578b\u8c37\u6c28\u9170\u80fa\u91761\uff08GLS1\uff09\u6216\u78f7\u9178\u9e7d\u6fc0\u6d3b\u7684\u7dda\u7c92\u9ad4\u8c37\u6c28\u9170\u80fa\u9176(phosphate-activated mitochondrial glutaminase) \u5c0d\u764c\u7d30\u80de\u5229\u7528\u8c37\u6c28\u9170\u80fa\u81f3\u95dc\u91cd\u8981\uff0c\u4e26\u4e14\u5df2\u77e5\u8c37\u6c28\u9170\u80fa\u9176\u7684\u901f\u7387\u5728\u816b\u7624\u4e2d\u589e\u52a0\uff0c\u4f7f\u5176\u5c0d\u816b\u7624\u7d30\u80de\u4ee3\u8b1d\u6975\u70ba\u91cd\u8981\u3002GLS1\u6291\u5236\u5291\u88ab\u8a8d\u70ba\u662f\u764c\u75c7\u6cbb\u7642(cancer therapy)\u7684\u6f5b\u5728\u5019\u9078\u85e5\u7269\u3002 BioVision\u7684\u8c37\u6c28\u9170\u80fa\u9176\uff08GLS1\uff09\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u7528\u65bc\u7be9\u9078GLS1\u6291\u5236\u5291\u4e4b\u87a2\u5149\u8a66\u5291\u76d2(plate-based)\u3002\u8c37\u6c28\u9170\u80fa\u6c34\u89e3\u8c37\u6c28\u9170\u80fa\u5f62\u6210\u8c37\u6c28\u9178\u548c\u6c28\u3002\u5728\u986f\u5f71\u5291\u548c\u9176\u6df7\u5408\u7269\u5b58\u5728\u4e0b\uff0c\u8c37\u6c28\u9178\u901a\u904e\u9176\u4fc3\u53cd\u61c9\u5c07\u975e\u87a2\u5149\u63a2\u91dd\u8f49\u5316\u70ba\u87a2\u5149\u7522\u7269\u3002\u63d0\u4f9b\u8c37\u6c28\u9170\u80fa\u9176\u6291\u5236\u5291-CB-839\u4f5c\u70ba\u6291\u5236\u5291\u5c0d\u7167\u3002<\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0|\u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668<\/strong> Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK479-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorometric (Ex\/Em = 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nScreening of potential inhibitors of Glutaminase<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Sensitive
\n\u2022 Specific assay for screening GLS1 inhibitors
\n\u2022 Includes hrGLS1 and CB-839 (inhibitor control)<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 GLS Probe
\n\u2022 GLS Developer
\n\u2022 GLS Enzyme Mix
\n\u2022 GLS Substrate
\n\u2022 GLS Assay Buffer
\n\u2022 GLS Dilution Buffer
\n\u2022 GLS1
\n\u2022 GLS Inhibitor (CB-839)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u8c37\u6c28\u9170\u80fa\u9176\uff08GLS1\uff09\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 | \u8ca8\u865fK479 Glutaminase (GLS1) Inhibit […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1937],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21920"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21920"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21920\/revisions"}],"predecessor-version":[{"id":21922,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21920\/revisions\/21922"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21920"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21920"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21920"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}