{"id":21912,"date":"2019-09-05T16:42:37","date_gmt":"2019-09-05T08:42:37","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21912"},"modified":"2022-07-20T10:48:39","modified_gmt":"2022-07-20T02:48:39","slug":"%e6%a0%b8%e5%9f%ba%e8%b3%aa%e8%9b%8b%e7%99%bd%e6%8f%90%e5%8f%96%e8%a9%a6%e5%8a%91%e7%9b%92detergent-free-invent%e8%b2%a8%e8%99%9fnm-033","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21912","title":{"rendered":"\u6838\u57fa\u8cea\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2(detergent-free) | Invent\u8ca8\u865fNM-033"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31<\/strong>:<\/strong> \u6838\u57fa\u8cea\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2(detergent-free) | Invent<\/strong>\u8ca8\u865f<\/strong>NM-033<\/strong><\/a><\/span><\/p>\n

Minute\u2122 Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells\/Tissues) (20 Preps)<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u6838\u8cea(Nucleoplasm)\uff0c\u4e5f\u7a31\u70ba\u6838\u8cea(karyoplasm )\/\u6838\u6db2(nucleus sap)\uff0c\u7531\u67d3\u8272\u9ad4(chromosomes)\uff0c\u6838\u4ec1(nucleolus)\u548c\u6838\u57fa\u8cea(nuclear matrix)\u7d44\u6210\u3002\u6838\u8cea (Nucleoplasm)\u5728\u907a\u50b3\u4fe1\u606f\u6d41(genetic information flow)\u548c\u57fa\u56e0\u8868\u9054\u8abf\u63a7\u4e2d\u8d77\u626e\u6f14\u91cd\u8981\u4f5c\u7528\u3002\u6838\u86cb\u767d\u5206\u96e2\u50b3\u7d71\u4e0a\u901a\u904e\u4f7f\u7528\u542b\u6709\u53bb\u6c61\u5291\u7684\u7de9\u885d\u6db2\u7684\u63d0\u53d6\u65b9\u6848\u4f86\u5b8c\u6210\uff0c\u5176\u6709\u6548\u5730\u63d0\u53d6\u7e3d\u6838\u86cb\u767d\u4f46\u4e0d\u80fd\u5206\u96e2\u819c\u7d50\u5408\u86cb\u767d\u548c\u6c34\u6eb6\u6027\u57fa\u8cea\u86cb\u767d\u3002\u4e0d\u542bDetergent\u7684\u6838\u57fa\u8cea\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2(The detergent-free nuclear matrix protein extraction kit)\uff0c\u4e3b\u8981\u4f7f\u7528\u7372\u5f97\u5c08\u5229\u7684\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u6280\u8853\u5c07\u7d30\u80de\u86cb\u767d\u5206\u6210\u4e09\u500b\u90e8\u5206\uff1a\u7d30\u80de\u6eb6\u8cea(cytosolic)\uff0c\u6838\u57fa\u8cea(nuclear matrix)\u548c\u6c34\u4e0d\u6eb6\u6027\u90e8\u5206(water-insoluble fractions) ; \u6c34\u4e0d\u6eb6\u6027\u90e8\u5206\u4e3b\u8981\u662f\u5305\u819c(envelope)\u548c\u6838\u9178\u76f8\u95dc\u86cb\u767d(nucleic acid-associated proteins)\u3002\u4f7f\u7528\u7684\u7de9\u6c96\u5291\u662f\u6d17\u6ecc\u5291(detergent)\u548c\u4e0d\u542bEDTA(EDTA-free)\u3002\u64cd\u4f5c\u6642\u9593\u4e0d\u52301\u5c0f\u6642\u5167\u5b8c\u6210\u3002\u63d0\u53d6\u7684\u86cb\u767d\u8cea\u8655\u65bc\u5176\u5929\u7136\u72c0\u614b(native state)\uff0c\u53ef\u7528\u65bc\u8a31\u591a\u4e0b\u6e38\u61c9\u7528\uff0c\u5982gel retardation\u3001\u8f49\u9304\u56e0\u5b50\u5206\u6790(transcription factor analysis)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u548c\u86cb\u767d\u8cea\u904b\u8f38(protein trafficking)\u7814\u7a76\u3002<\/p>\n

\"\"<\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n
Items<\/strong><\/td>\nQuantity<\/strong><\/td>\n<\/tr>\n
Buffer A<\/td>\n30 ml<\/td>\n<\/tr>\n
Buffer B<\/td>\n5 ml<\/td>\n<\/tr>\n
Protein Extraction Filter Cartridges<\/td>\n20 units<\/td>\n<\/tr>\n
Plastic Rods<\/td>\n2 units<\/td>\n<\/tr>\n
Collection Tubes<\/td>\n20 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1 \u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | \u8cea\u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a> |<\/p>\n","protected":false},"excerpt":{"rendered":"

\u7522\u54c1\u540d\u7a31: \u6838\u57fa\u8cea\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2(detergent-free) | Invent\u8ca8\u865fNM-033 Minut […]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1939],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21912"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21912"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21912\/revisions"}],"predecessor-version":[{"id":21913,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21912\/revisions\/21913"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21912"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21912"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21912"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}