{"id":21883,"date":"2019-09-02T13:51:16","date_gmt":"2019-09-02T05:51:16","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21883"},"modified":"2022-09-08T16:26:55","modified_gmt":"2022-09-08T08:26:55","slug":"goat-anti-human-igg-monkey-ads-unlb%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f2049-01","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21883","title":{"rendered":"Goat Anti-Human IgG, Monkey ads-UNLB\u6297\u9ad4 | \u8ca8\u865f2049-01"},"content":{"rendered":"

Goat Anti-Human IgG, Monkey ads-UNLB\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>2049-01 <\/strong><\/a><\/span>\u898f\u683c<\/strong>1.0 mg \u00a0(1.0 mg\/mL)<\/p>\n

\"SB\"<\/a><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0Goat Anti-Human IgG, Monkey ads-UNLB\u6297\u9ad4\uff0c\u4e0d\u5177\u6a19\u8a8c\u3002\u91dd\u5c0dhuman IgG\u91cd\u93c8(heavy chain)\u5177\u7279\u7570\u6027\u3002\u4f86\u6e90\u70baPooled antisera from goats hyperimmunized with human IgG\u3002Isotype\u70baGoat IgG\u3002Isotype Control Goat IgG-UNLB<\/a>\u3002 \u7de9\u885d\u6db2\u914d\u65b9Borate buffered saline, pH 8.2\u3002<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n

ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-UNLB (SB Cat. No. 2049-01) followed by Swine Anti-Goat IgG(H+L), Human\/Rat\/Mouse SP ads-HRP (SB Cat. No. 6300-05).<\/div>\n
\"\"<\/div>\n
<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Specificity<\/th>\nReacts with the heavy chain of human IgG<\/td>\n<\/tr>\n
Source<\/th>\nPooled antisera from goats hyperimmunized with human IgG<\/td>\n<\/tr>\n
Cross Adsorption<\/th>\nHuman IgM and IgA; rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) IgG; may react with IgG from other species<\/td>\n<\/tr>\n
Purification<\/th>\nAffinity chromatography on human IgG covalently linked to agarose<\/td>\n<\/tr>\n
Isotype<\/th>\nGoat IgG<\/td>\n<\/tr>\n
Isotype Control<\/th>\nGoat IgG-UNLB<\/a><\/td>\n<\/tr>\n
Format\/Conjugate<\/th>\nUNLB (Unconjugated)<\/td>\n<\/tr>\n
Buffer Formulation<\/th>\nBorate buffered saline, pH 8.2<\/td>\n<\/tr>\n
Concentration<\/th>\n1.0 mg\/mL<\/td>\n<\/tr>\n
Volume<\/th>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/th>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/th>\n\n
Quality tested applications for relevant formats include –<\/div>\n
ELISA
\nFLISA<\/div>\n
Other referenced applications for relevant formats include –<\/div>\n
Immunoprecipitation\u00a01,2<\/sup><\/div>\n<\/td>\n<\/tr>\n
Recommended Dilutions<\/th>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/th>\nAB_2795694<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e8c\u7d1a\u6297\u9ad4 (Secondary antibodies) \u61c9\u7528:<\/p>\n

\u4e8c\u6297(Secondary antibodies)\u662f\u91dd\u5c0d\u514d\u75ab\u7403\u86cb\u767d\uff08immunoglobulin , Ig\uff09\u5206\u5b50\u7684\u591a\u514b\u9686(polyclonal) \u548c\u55ae\u514b\u9686\u6297\u9ad4(monoclonal antibodies)\u3002\u5b83\u5011\u901a\u5e38\u8207\u6297\u539f\u7279\u7570\u6027\u4e00\u6297(specific primary antibody) \u4e00\u8d77\u4f7f\u7528\uff0c\u4e26\u4e14\u901a\u5e38\u88ab\u6a19\u5b9a\u9175\u7d20\u6216\u87a2\u5149\u7269\u8cea\u4ee5\u5e6b\u52a9\u6aa2\u6e2c\u3002\u4e8c\u6297\u53ef\u7528\u65bc\u591a\u7a2e\u61c9\u7528\uff0c\u4f8b\u5982ELISA\u3001\u514d\u75ab\u5370\u8de1(Immunoblotting)\u3001\u6d41\u5f0f\u7d30\u80de\u5100(Flow Cytometry)\u3001\u514d\u75ab\u7d44\u7e54\u67d3\u8272(Immunohistochemistry)\u3001\u514d\u75ab\u7d30\u80de\u5316\u5b78(Immuocytochemistry)\u3002\u4e8c\u7d1a\u6297\u9ad4\u901a\u5e38\u7528\u9176\u6216\u87a2\u5149\u67d3\u6599\u6a19\u8a18\uff0c\u9019\u4f7f\u5f97\u80fd\u5920\u901a\u904e\u6bd4\u8272\uff0c\u5316\u5b78\u767c\u5149\u548c\u87a2\u5149\u6280\u8853\u9032\u884c\u6aa2\u6e2c\u3002\u4f8b\u5982\uff0c\u9176\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08enzymes horseradish peroxidase , HRP\uff09\u548c\u9e7c\u6027\u78f7\u9178\u9176\uff08alkaline phosphatase , AP\uff09\u901a\u904e\u6bd4\u8272\u548c\u5316\u5b78\u767c\u5149\u5e73\u53f0\u5728ELISA\u548c\u86cb\u767d\u8cea\u5370\u8de1\u61c9\u7528\u4e2d\u662f\u6709\u50f9\u503c\u7684\u3002\u87a2\u5149\u86cb\u767d\u8af8\u5982\u87a2\u5149\u7d20\uff08fluorescein , FITC\uff09\uff0c\u85fb\u7d05\u86cb\u767d\uff08phycoerythrin , PE\uff09\u548c\u82b1\u9752\uff08cyanine , CY\uff09\u7684\u87a2\u5149\u67d3\u6599\u5728\u87a2\u5149\u7684\u61c9\u7528\u4e2d\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\uff0c\u5305\u62ec\u6d41\u5f0f\u7d30\u80de\u8853\uff0c\u514d\u75ab\u87a2\u5149\u986f\u5fae\u8853\u548c\u591a\u91cd\u6e2c\u5b9a\u3002\u4e8c\u6297\u5141\u8a31\u6a19\u8a18\u548c\u6aa2\u6e2c\u7684\u9748\u6d3b\u6027\u4ee5\u53ca\u901a\u904e\u4fe1\u865f\u653e\u5927\uff0c\u53ef\u589e\u52a0\u7684\u9748\u654f\u5ea6\u3002
\nSBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/span><\/p>\n

\u5f15\u7528\u6587\u737bReferences<\/p>\n

    \n
  1. Zhang Q, Spellman DS, Song Y, Choi B, Hatcher NG, Tomazela D, et al. Generic automated method for liquid chromatography-multiple reaction monitoring mass spectrometry based monoclonal antibody quantitation for preclinical pharmacokinetic studies. Anal Chem. 2014;86:8776-84. (IP)<\/li>\n
  2. Schroeder KM, Beyer TP, Hansen RJ, Han B, Pickard RT, Wroblewski VJ, et al. Proteolytic cleavage of antigen extends the durability of an anti-PCSK9 monoclonal antibody. J Lipid Res. 2015 44075. pii: jlr.M061903. [Epub ahead of print]. (IP)<\/li>\n<\/ol>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Goat Anti-Human IgG, Monkey ads-UNLB\u6297\u9ad4 | \u8ca8\u865f2049-01 \u898f\u683c1. […]<\/p>\n","protected":false},"author":3,"featured_media":34065,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[1761,1948],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21883"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21883"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21883\/revisions"}],"predecessor-version":[{"id":34066,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21883\/revisions\/34066"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34065"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21883"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21883"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21883"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}