{"id":21846,"date":"2019-08-29T14:06:12","date_gmt":"2019-08-29T06:06:12","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21846"},"modified":"2022-07-20T10:50:05","modified_gmt":"2022-07-20T02:50:05","slug":"%e7%9a%ae%e8%86%9a%e7%b5%84%e7%b9%94%e8%9b%8b%e7%99%bd%e8%b3%aa%e8%90%83%e5%8f%96-minute-total-protein-extraction-kit-for-skin-tissue-%e8%b2%a8%e8%99%9fsa-01-sk","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21846","title":{"rendered":"\u76ae\u819a\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6 | Minute\u2122 Total Protein Extraction Kit for Skin Tissue \u8ca8\u865fSA-01-SK"},"content":{"rendered":"<p><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=21846\">\u76ae\u819a\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44 | Minute\u2122 Total Protein Extraction Kit for Skin Tissue \u8ca8\u865fSA-01-SK<\/a><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/products\/SA-01-SK_1024x1024_2fb41b3f-8a34-4805-867f-390b4897d813_1024x1024@2x.png?v=1541101102\" alt=\"\" width=\"378\" height=\"606\" \/><\/p>\n<p>\u7522\u54c1\u4ecb\u7d39: \u76ae\u819a\u7d44\u7e54(Skin tissue)\u7531\u8868\u76ae(epidermis)\uff0c\u771f\u76ae(dermis)\u548c\u76ae\u4e0b\u8102\u80aa(subcutaneous fat)\u7d44\u6210\u3002\u7531\u65bc\u5176\u7368\u7279\u7684\u7d50\u69cb(unique structure)\uff0c\u773e\u6240\u5468\u77e5\uff0c\u76ae\u819a\u7d44\u7e54\u96e3\u4ee5\u5747\u8cea\u5316\u3002\u88c2\u89e3\u7d44\u7e54\u4e2d\u7684\u7d30\u80de\u4ee5\u9032\u884c\u7e3d\u86cb\u767d\u8cea\u63d0\u53d6\u4e5f\u662f\u975e\u5e38\u56f0\u96e3\u7684\u3002\u50b3\u7d71\u7684\u57fa\u65bc\u6eb6\u6db2\u7684\u86cb\u767d\u8cea\u63d0\u53d6\u65b9\u6cd5\uff0c\u4f8b\u5982RIPA\uff0c\u6548\u7387\u4f4e\uff0c\u7522\u91cf\u975e\u5e38\u4f4e\u3002\u4f7f\u7528\u57fa\u65bc\u6eb6\u6db2\u7684\u65b9\u6cd5\uff0c\u63d0\u53d6\u7684\u86cb\u767d\u8cea\u7684\u5206\u4f48\u4e5f\u662f\u4e0d\u5b8c\u6574\u7684\u3002\u8a72\u8a66\u5291\u76d2\u901a\u904e\u7d50\u5408\u6a5f\u68b0\u63d0\u53d6(mechanical extraction) \u548c\u76ae\u819a\u7d44\u7e54\u7684\u5316\u5b78\u88c2\u89e3(chemical lysis)\uff0c\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5f9e\u4eba\u6216\u52d5\u7269\u76ae\u819a\u7d44\u7e54\u4e2d\u63d0\u53d6\u7e3d\u86cb\u767d\u7684\u9ad8\u6548\u65b9\u6cd5\u3002\u8a72\u8a66\u5291\u76d2\u5177\u6709\u7c21\u55ae\u5feb\u901f\u7684\u55ae\u7ba1\u65b9\u6848\u548c\u512a\u5316\u7684\u76ae\u819a\u7d44\u7e54\u7de9\u885d\u6db2\u3002\u7814\u7a76\u4eba\u54e1\u53ef\u4ee5\u9078\u64c7\u8b8a\u6027(denaturing)\u6216\u5929\u7136(native)\u7d30\u80de\u88c2\u89e3\u7de9\u885d\u6db2\uff0c\u9019\u662f\u5c08\u70ba\u76ae\u819a\u7d44\u7e54\u5b9a\u5236\u7684\u3002\u6574\u500b\u904e\u7a0b\u9700\u8981\u4e0d\u523010\u5206\u9418\u5b8c\u6210\uff0c\u86cb\u767d\u8cea\u7522\u91cf\u57281-5\u6beb\u514b\/\u6beb\u5347\u7684\u7bc4\u570d\u5167\u3002\u63d0\u4f9b\u7684\u6750\u6599\u8db3\u4ee5\u9032\u884c50\u6b21\u63d0\u53d6\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/SA-01_grande.jpg?v=1541178029\" alt=\"\" \/><\/p>\n<p><strong>Minute\u2122<\/strong><strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong><strong>Column Filter<\/strong><strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong><strong>\u00a0<\/strong><strong>\u4e00<\/strong> <strong>\u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong><strong><em>vortex<\/em><\/strong><strong><em>\u3001<\/em><\/strong><strong><em>transfer<\/em><\/strong><strong><em>\u3001<\/em><\/strong><strong><em>spin <\/em><\/strong><strong><em>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>Items<\/strong><\/td>\n<td><strong>Quantity<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Denaturing Buffer<\/td>\n<td>25 ml<\/td>\n<\/tr>\n<tr>\n<td>Native Buffer<\/td>\n<td>25 ml<\/td>\n<\/tr>\n<tr>\n<td>Protein Extraction Powder<\/td>\n<td>5g<\/td>\n<\/tr>\n<tr>\n<td>Plastic Rods<\/td>\n<td>2 units<\/td>\n<\/tr>\n<tr>\n<td>Filter Cartridges<\/td>\n<td>50 units<\/td>\n<\/tr>\n<tr>\n<td>Collection Tubes<\/td>\n<td>50 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>\u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1 <span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7531\">\u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7484\">\u8cea\u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a> |<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u76ae\u819a\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44 | Minute\u2122 Total Protein Extraction Kit for  [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1952],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21846"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21846"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21846\/revisions"}],"predecessor-version":[{"id":21847,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21846\/revisions\/21847"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21846"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21846"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21846"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}