{"id":21808,"date":"2019-08-28T11:01:55","date_gmt":"2019-08-28T03:01:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21808"},"modified":"2023-07-10T17:05:03","modified_gmt":"2023-07-10T09:05:03","slug":"%e7%a3%b7%e9%85%b8%e6%9e%9c%e7%b3%96%e6%bf%80%e9%85%b6%ef%bc%88pfk%ef%bc%89%e6%b4%bb%e6%80%a7%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk776","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21808","title":{"rendered":"\u78f7\u9178\u679c\u7cd6\u6fc0\u9176\uff08PFK\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | Phosphofructokinase (PFK) Activity Colorimetric Assay Kit\u8ca8\u865fk776 (abcam ab155898)"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=21808\">\u78f7\u9178\u679c\u7cd6\u6fc0\u9176\uff08PFK\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 <strong>| <\/strong><strong>\u8ca8\u865f<\/strong>k776 (abcam ab155898)<\/a><\/span><\/p>\n<p><strong><em>Phosphofructokinase (PFK) Activity Colorimetric Assay Kit (&lt; 1 mU\/well)<\/em><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/7\/k776.jpg\" alt=\"\" \/><\/p>\n<p><strong>\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong> \u78f7\u9178\u679c\u7cd6\u6fc0\u9176\uff08Phosphofructokinase ,PFK\uff09\u662f\u4e00\u7a2e\u95dc\u9375\u7684\u7cd6\u9175\u89e3\u9176\uff0c\u5728\u7cd6\u9175\u89e3(glycolysis)\u904e\u7a0b\u4e2d\u8d77\u8457\u91cd\u8981\u7684\u8abf\u7bc0\u4f5c\u7528\u3002\u8a72\u9176\u5b58\u5728\u65bc\u7d30\u83cc(bacteria)\uff0c\u690d\u7269(plants)\u548c\u52d5\u7269(animals)\u4e2d\u3002\u6709\u5169\u7a2e\u985e\u578b\u7684PFK\u00a0 &#8211;\u00a0 PFK1\u548cPFK2\u3002\u5728ATP\u5b58\u5728\u4e0b\uff0cPFK1\u548cPFK2\u5206\u5225\u50ac\u5316\u679c\u7cd6-6-\u78f7\u9178\u8f49\u5316\u70ba\u679c\u7cd6-1,6-\u4e8c\u78f7\u9178\u548c\u679c\u7cd6-2,6-\u4e8c\u78f7\u9178\u548cADP\u3002 PFK\u5728\u54fa\u4e73\u52d5\u7269\u4e2d\u67093\u7a2e\u4e3b\u8981\u7684\u540c\u7a2e\u578b\uff1aPFK-M\uff08\u808c\u8089\uff09\uff0cPFK-L\uff08\u809d\u81df\uff09\u548cPFK-P\uff08\u8840\u5c0f\u677f\uff09\u3002\u5728\u4eba\u985e\u4e2d\uff0cPFK\u7f3a\u4e4f\u5c0e\u81f4\u7cd6\u539f\u8caf\u7a4d\u75c5\uff0c\u4e5f\u7a31\u70baTarui\u75c5\uff0c\u5176\u7279\u5fb5\u5728\u65bc\u904b\u52d5\u5f15\u8d77\u7684\u808c\u8089\u7121\u529b\u548c\u75d9\u6523\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u589e\u52a0\u7684PFK\u6d3b\u6027\u6709\u52a9\u65bc\u764c\u7d30\u80de\u589e\u6b96\u548c\u81f4\u816b\u7624\u6027\u3002\u65e9\u671f\u6aa2\u6e2c\u7570\u5e38\u7684\u78f7\u9178\u679c\u7cd6\u6fc0\u9176\u6d3b\u6027\u5c0d\u65bc\u8a3a\u65b7\uff0c\u9810\u6e2c\u548c\u6cbb\u7642\u7b56\u7565\u662f\u81f3\u95dc\u91cd\u8981\u7684\u3002\u5728BioVision\u7684\u78f7\u9178\u679c\u7cd6\u6fc0\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u4e2d\uff0cPFK\u5c07\u679c\u7cd6-6-\u78f7\u9178\u548cATP\u8f49\u5316\u70ba\u679c\u7cd6\u4e8c\u78f7\u9178\u548cADP\u3002\u5728\u5e95\u7269\u548c\u9176\u6df7\u5408\u7269\u5b58\u5728\u4e0b\u7684ADP\u8f49\u5316\u70baAMP\u548cNADH\uff0c\u5176\u5c07\u7121\u8272\u63a2\u91dd\u9084\u539f\u6210\u6709\u8272\u7522\u7269\uff0c\u5728450nm\u8655\u5177\u6709\u5f37\u5438\u5149\u5ea6\u3002 PFK\u6d3b\u6027\u6e2c\u5b9a\u7c21\u55ae\uff0c\u7a69\u5065\u4e14\u5feb\u901f\uff0c\u53ef\u6aa2\u6e2c\u78f7\u9178\u679c\u7cd6\u6fc0\u9176\u6d3b\u6027\u5c0f\u65bc1 mU\u3002<\/p>\n<p>\u5f15\u7528\u6587\u737b<\/p>\n<p>Thurley, Kevin et al. (2017) Principles for circadian orchestration of metabolic pathways,\u00a0<a href=\"http:\/\/www.pnas.org\/content\/114\/7\/1572.abstract?sid=e8f1c9aa-147d-4daa-a058-7d4e0c684921\">Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1572-1577.<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u78f7\u9178\u679c\u7cd6\u6fc0\u9176\uff08PFK\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fk776 (abcam ab155898) Phosphof [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1954],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21808"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21808"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21808\/revisions"}],"predecessor-version":[{"id":43898,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21808\/revisions\/43898"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21808"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21808"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21808"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}