{"id":21805,"date":"2019-08-28T10:28:54","date_gmt":"2019-08-28T02:28:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21805"},"modified":"2022-07-20T10:50:43","modified_gmt":"2022-07-20T02:50:43","slug":"%e4%b8%99%e9%85%ae%e9%85%b8%e6%af%94%e8%89%b2%e8%9e%a2%e5%85%89%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk609-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21805","title":{"rendered":"\u4e19\u916e\u9178\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c | Pyruvate Colorimetric\/Fluorometric Assay Kit\u8ca8\u865fK609-100"},"content":{"rendered":"

\u4e19\u916e\u9178\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K609-100<\/a><\/span><\/p>\n

Pyruvate Colorimetric\/Fluorometric Assay Kit<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u4e19\u916e\u9178(Pyruvate)\u662f\u4ee3\u8b1d\u4e2d\u7684\u4e2d\u5fc3\u5206\u5b50\uff0c\u7cd6\u901a\u904e\u8a72\u5206\u5b50\u9032\u5165\u6ab8\u6aac\u9178\u5faa\u74b0(citric acid cycle)\u3002 \u4e19\u916e\u9178(Pyruvate)\u53ef\u5728\u7cd6\u7570\u751f\u904e\u7a0b\u4e2d\u8f49\u5316(converted)\u70ba\u78b3\u6c34\u5316\u5408\u7269(carbohydrates)\uff0c\u6216\u901a\u904e\u4e59\u9170\u8f14\u9176A\u8f49\u5316(acetyl CoA)\u70ba\u8102\u80aa\u9178(fatty acids)\u3002 \u9ad8\u6c34\u5e73\u7684\u4e19\u916e\u9178(pyruvate)\u8207\u809d\u81df\u75be\u75c5(liver disease)\u548c\u907a\u50b3\u6027\u75be\u75c5(genetic disorders)\u6709\u95dc\u3002 \u4e19\u916e\u9178\u4e5f\u88ab\u7528\u65bc\u523a\u6fc0\u4ee3\u8b1d\u5c0e\u81f4\u9ad4\u91cd\u6e1b\u8f15\u3002 BioVision\u63d0\u4f9b\u7c21\u55ae\uff0c\u76f4\u63a5\u548c\u81ea\u52d5\u5316(automation-ready)\u7684\u7a0b\u5e8f\uff0c\u7528\u65bc\u6e2c\u91cf\u5404\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u7684\u4e19\u916e\u9178\u6fc3\u5ea6(pyruvate concentration)\uff0c\u5982\u8840\u6db2(blood)\uff0c\u7d30\u80de(cells)\uff0c\u57f9\u990a\u7269(culture)\u548c\u767c\u9175\u57f9\u990a\u57fa(fermentation media)\u7b49\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u4e19\u916e\u9178\u88ab\u4e19\u916e\u9178\u6c27\u5316\u9176(pyruvate oxidase)\u901a\u904e\u9176\u53cd\u61c9\u6c27\u5316\u7522\u751f\u984f\u8272\uff08\u03bb = 570nm\uff09\u548c\u87a2\u5149\uff08Ex \/ Em = 535 \/ 587nm\uff09\u3002 \u7531\u65bc\u984f\u8272\u87a2\u5149\u5f37\u5ea6\u8207\u4e19\u916e\u9178(Pyruvate)\u542b\u91cf\u6210\u6bd4\u4f8b\uff0c\u56e0\u6b64\u53ef\u4ee5\u7cbe\u78ba\u5730\u6e2c\u91cf\u4e19\u916e\u9178(Pyruvate)\u6fc3\u5ea6\u3002 \u8a72\u8a66\u5291\u76d2\u6aa2\u6e2c1\u03bcM\u81f310 mM\u4e19\u916e\u9178(Pyruvate)\u3002<\/p>\n

\u5546\u54c1\u9700\u7528\u5100\u5668\u6e2c\u91cf<\/span>\u00a0\u00a0\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100\u8acb\u6309\u6b64\u00a0<\/a><\/span>(\u6a19\u914d405\u3001450\u3001492\u3001630 nm, \u53ef\u64f4\u589e\u81f37\u500b\u6ffe\u7247)<\/p>\n

\"RT-6900\"<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/strong>\u6aa2\u6e2c<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK609-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (570 nm) or Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit detects 1 \u00b5M to 10 mM pyruvate.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~40 minutes
\n\u2022 Fast and convenient
\n\u2022 Kit contains all necessary reagents for accurate measurement of pyruvate concentrations in biological samples<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Pyruvate Assay Buffer
\n\u2022 Pyruvate Probe (in DMSO)
\n\u2022 Pyruvate Enzyme Mix
\n\u2022 Pyruvate Standard (100 nmol\/\u00b5l)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Qi Wu, et al., ( 2018) Tumour-originated exosomal miR-155 triggers cancer-associated cachexia to promote tumour progression,\u00a0Molecular Cancer, Oct. 2018, 30359265<\/a><\/p>\n

Ana I. Duarte, et al., (2018) Dual Therapy with Liraglutide and Ghrelin Promotes Brain and Peripheral Energy Metabolism in the R6\/2 Mouse Model of Huntington\u2019s Disease,\u00a0Scientific Reports, Jun. 2018, 29895889<\/a><\/p>\n

Chem et al., Mitochondrial Citrate Transporter-dependent Metabolic Signature in the 22q11.2 Deletion Syndrome.\u00a0J. Biol. Chem.,\u00a0Sep 2015; 290: 23240 – 23253.<\/a><\/p>\n

Kim et al., Mice expressing reduced levels of hepatic glucose-6-phosphatase-\u03b1 activity do not develop age-related insulin resistance or obesity.\u00a0 Hum. \u00a0Mol. Genet.,\u00a0Sep 2015; 24: 5115 – 5125.<\/a><\/p>\n

Chen et al., Cadmium toxicity induces ER stress and apoptosis via impairing energy homoeostasis in cardiomyocytes.\u00a0Biosci. Rep., Jun 2015; 35: e00214.<\/a><\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e19\u916e\u9178\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK609-100 Pyruvate Colorimetric\/Fluoro […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1955],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21805"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21805"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21805\/revisions"}],"predecessor-version":[{"id":26654,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21805\/revisions\/26654"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21805"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21805"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21805"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}