{"id":21736,"date":"2019-08-22T14:45:50","date_gmt":"2019-08-22T06:45:50","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21736"},"modified":"2025-07-07T10:55:19","modified_gmt":"2025-07-07T02:55:19","slug":"elisa-ultrasensitive-tmb-substrate-for-hrp-%e8%b2%a8%e8%99%9f-g444","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21736","title":{"rendered":"TMB Substrate | ELISA Ultrasensitive TMB Substrate for HRP\u8ca8\u865f G444"},"content":{"rendered":"

ELISA Ultrasensitive TMB Substrate for HRP<\/span><\/span><\/span><\/strong> | <\/strong>\u8ca8\u865f<\/strong> G444<\/span><\/a><\/span>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/strong><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39: 3,3’5,5′-Tetramethylbenzidine (TMB) is a safe-sensitive substrate for the detection of horseradish peroxidase (HRP) activity. It yields a blue color (Amax = 652nm) and changes to yellow (Amax = 450nm) upon the addition of either sulfuric or phosphoric acid. ABM\u2019s ELISA Ultrasensitive TMB Substrate for HRP yields the greatest sensitivity among the TMB substrates. 3,3’5,5′-\u56db\u7532\u57fa\u806f\u82ef\u80fa\uff08,3’5,5′-Tetramethylbenzidine , TMB\uff09\u662f\u4e00\u7a2e\u5b89\u5168\u654f\u611f\u7684\u5e95\u7269\uff0c\u7528\u65bc\u6aa2\u6e2c\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08HRP\uff09\u6d3b\u6027\u3002 \u7576\u52a0\u5165\u786b\u9178\u6216\u78f7\u9178\u6642\uff0c\u5b83\u7522\u751f\u85cd\u8272\uff08Amax = 652nm\uff09\u4e26\u8b8a\u70ba\u9ec3\u8272\uff08Amax = 450nm\uff09\u3002 ABM\u7528\u65bcHRP\u7684ELISA\u8d85\u654f\u611fTMB\u5e95\u7269\u5728TMB\u5e95\u7269\u4e2d\u7522\u751f\u6700\u5927\u7684\u9748\u654f\u5ea6\u3002<\/p>\n

\u5176\u5b83<\/strong>\u5546\u54c1\u00a0<\/strong>AKT2<\/u><\/strong>\u00a0<\/u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/a> | <\/strong>EGFR<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>HER2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>LIMK1<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>MEK1 (EE)<\/u><\/strong>\u00a0<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>\u00a0<\/u><\/strong>ROCK2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>ROS1 (G2032R)<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/span><\/p>\n\n\n\n\n\n\n
escription<\/strong><\/td>\n3,3’5,5′-Tetramethylbenzidine (TMB) is a safe-sensitive substrate for the detection of horseradish peroxidase (HRP) activity. It yields a blue colour (Amax = 652nm) and changes to yellow (Amax = 450nm) upon the addition of either sulfuric or phosphoric acid. ABM\u2019s ELISA Ultrasensitive TMB Substrate for HRP yields the greatest sensitivity among the TMB substrates.<\/td>\n<\/tr>\n
Protocol<\/strong><\/td>\n\n

1. Complete all required incubations with antibodies, probes and HRP labeled reagents.<\/p>\n

2. Wash plate, at least 4 times, with phosphate buffered saline or Tris buffered saline containing 0.1% Tween-20.<\/p>\n

3. After the final wash, shake and blot all residual buffers from plate wells.<\/p>\n

4. Add 0.1ml of ABM\u2019s Ultrasensitive TMB Substrate for HRP to appropriate wells and incubate 5-30 minutes.<\/p>\n

5. The reaction can be monitored as a function of time for kinetic assays or stopped with 0.1ml of 0.3mol\/l of sulphuric acid or 0.1% sodium fluoride and read at 650nm.<\/p>\n

6. If reaction needs to be converted to yellow diimine, add 0.1ml of either sulfuric or phosphoric acid and record the absorbance within 5 minutes.<\/td>\n<\/tr>\n

Application<\/strong><\/td>\nSuitable for kinetic and endpoint assays involving Horseradish Peroxidase (HRP).<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nStore at room temperature (15-28\u00b0C) or at 2-4\u00b0C and then warm up to assay temperature prior to use. Prevent exposure to direct sunlight. Discard if solution turns blue or turbid.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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ELISA Ultrasensitive TMB Substrate for HRP | \u8ca8\u865f G444\u00a0\u00a0\u00a0 […]<\/p>\n","protected":false},"author":3,"featured_media":2639,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1961],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21736"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21736"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21736\/revisions"}],"predecessor-version":[{"id":51990,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21736\/revisions\/51990"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2639"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21736"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21736"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21736"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}