{"id":21734,"date":"2019-08-22T11:46:52","date_gmt":"2019-08-22T03:46:52","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21734"},"modified":"2022-10-19T14:09:20","modified_gmt":"2022-10-19T06:09:20","slug":"tmb-3355-%e5%9b%9b%e7%94%b2%e5%9f%ba%e8%81%af%e8%8b%af%e8%83%ba-%e8%b2%a8%e8%99%9f1215-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21734","title":{"rendered":"(TMB) 3,3’5,5′-\u56db\u7532\u57fa\u806f\u82ef\u80fa| TMB, Peroxidase substrate (Ultrasensitive)\u8ca8\u865f1215-100 (Abcam ab142042)"},"content":{"rendered":"

(TMB) 3,3’5,5′-\u56db\u7532\u57fa\u806f\u82ef\u80fa| \u00a0TMB, Peroxidase substrate (Ultrasensitive)<\/strong>\u8ca8\u865f<\/strong>1215-100<\/a>\u00a0\u00a0(Abcam ab142042)<\/span><\/p>\n

TMB, Ultrasensitive<\/em><\/strong><\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

3,3’5,5′-\u56db\u7532\u57fa\u806f\u82ef\u80fa\uff083,3’5,5′-Tetramethylbenzidine , TMB\uff09\u662f\u7528\u65bc\u6e2c\u5b9a\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08horseradish peroxidase , HRP\uff09\u7684\u5b89\u5168\u654f\u611f\u5e95\u7269\u3002\u6700\u521d\uff0c\u5728HRP\u548c\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\u5b58\u5728\u4e0b\uff0c\u5f62\u6210\u55ae\u96fb\u5b50\u6c27\u5316\u7522\u7269(one-electron oxidation product)\u3002\u8a72\u5316\u5408\u7269\u662f\u967d\u96e2\u5b50\u81ea\u7531\u57fa(cation free radical)\uff0c\u70ba\u85cd\u8272\uff0c\u6700\u5927\u5438\u9644\u5728653nm\u3002\u8207HRP \/ H 2 O 2\u7684\u9032\u4e00\u6b65\u53cd\u61c9\u6216\u7528\u9178\u9178\u5316\u81ea\u7531\u57fa\u7522\u751f\u4e8c\u4e9e\u80fa\u672b\u7aef\u6c27\u5316\u7522\u7269\u5728450nm\u8655\u5438\u6536\u5149\u3002\u81ea\u7531\u57fa\u7684\u6d88\u5149\u4fc2\u6578\uff08E653 nm = 3.9×10\u2074mo-1 cm -1\uff09\u548c\u4e8c\u4e9e\u80fa\uff08E450 nm = 5.9 x 10 6 mol -1 cm -1\uff09\u70baHRP\u548cHRP\u6a19\u8a18\u63a2\u91dd\u7684\u6e2c\u5b9a\u63d0\u4f9b\u4e86\u975e\u5e38\u9748\u654f\u7684\u7cfb\u7d71\u3002Biovision Inc.\u7372\u5f97\u7684TMBUS\u662f\u4e00\u7a2e\u5728\u5ba4\u6eab\u4e0b\u7a69\u5b9a\u4e14\u5c0d\u666e\u901a\u5be6\u9a57\u5ba4\u5149\u4e0d\u654f\u611f\u7684\u55ae\u7d44\u5206\u8a66\u5291\u3002\u5b83\u91dd\u5c0dTMB\u548c\u904e\u6c27\u5316\u6c2b\u6fc3\u5ea6\u9032\u884c\u4e86\u512a\u5316\uff0c\u4e26\u4e14\u901a\u5e38\u5728\u514d\u75ab\u6e2c\u5b9a\u4e2d\u4f7f\u7528HRP\u6fc3\u5ea6\u7522\u751f\u7dda\u6027\u97ff\u61c9\u3002<\/p>\n

\"\u64cd\u4f5c\u624b\u518a2\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\n1215-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 ml<\/td>\n<\/tr>\n
Alternate Name<\/strong><\/td>\n3,3′,5,5′-Tetramethylbenzidine; TMBUS<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\n2.08 mMol L\u207b\u00b9 and Hydrogen Peroxide, citric acid buffer at pH 3.3.<\/td>\n<\/tr>\n
CAS #<\/strong><\/td>\n54827-17-7<\/td>\n<\/tr>\n
Molecular Formula<\/strong><\/td>\nC\u2081\u2086H\u2082\u2080N\u2082<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n240.35<\/td>\n<\/tr>\n
SMILES<\/strong><\/td>\nCC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C<\/td>\n<\/tr>\n
InChi<\/strong><\/td>\nInChI=1S\/C16H20N2\/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14\/h5-8H,17-18H2,1-4H3<\/td>\n<\/tr>\n
InChi Key<\/strong><\/td>\nUAIUNKRWKOVEESUHFFFAOYSA-N<\/td>\n<\/tr>\n
PubChem CID<\/strong><\/td>\n41206<\/a><\/td>\n<\/tr>\n
MDL Number<\/strong><\/td>\nMFCD00007748<\/td>\n<\/tr>\n
Handling<\/strong><\/td>\nProtect from light<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u71b1\u92b7\u5100\u5668\"<\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a>\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a>\"ELISA<\/a><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

(TMB) 3,3’5,5′-\u56db\u7532\u57fa\u806f\u82ef\u80fa| \u00a0TMB, Peroxidase sub […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1962,1963,1964],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21734"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21734"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21734\/revisions"}],"predecessor-version":[{"id":34966,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21734\/revisions\/34966"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21734"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21734"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21734"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}