{"id":21690,"date":"2019-08-20T14:53:29","date_gmt":"2019-08-20T06:53:29","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21690"},"modified":"2022-07-20T10:51:42","modified_gmt":"2022-07-20T02:51:42","slug":"trueprime%e5%96%ae%e7%b4%b0%e8%83%9ewga%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9f351100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21690","title":{"rendered":"TruePrime\u00ae\u55ae\u7d30\u80deWGA\u8a66\u5291\u76d2 | \u8ca8\u865f351100"},"content":{"rendered":"

TruePrime\u00ae\u55ae\u7d30\u80deWGA\u8a66\u5291\u76d2\u00a0|\u00a0<\/strong>\u8ca8\u865f<\/strong>351100<\/strong><\/a><\/span><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong>\u00a0TruePrime\u00ae\u55ae\u7d30\u80deWGA\u8a66\u5291\u76d2\u63a1\u7528\u4e86\u4e00\u7a2e\u65b0\u7a4e\u53ef\u9760\u7684\u65b9\u6cd5\uff0c\u53ef\u4ee5\u5f9e\u55ae\u500b\u7d30\u80de\u6216\u66f4\u5c11\u7684\u7d30\u80de\u4e2d\u5be6\u73fe\u6e96\u78ba\u7684\u57fa\u56e0\u7d44\u64f4\u589e(genome amplification)\u3002\u5982\u679c\u60a8\u7684\u5be6\u9a57\u8a2d\u8a08\u5141\u8a31\uff0c\u653e\u5927\u4e00\u4e9b\u7d30\u80de\u800c\u4e0d\u662f\u4e00\u500b\u7d30\u80de\u53ef\u4ee5\u63d0\u9ad8total coverage breadth\u3002TruePrime\u00ae\u55ae\u7d30\u80deWGA\u8a66\u5291\u76d2\u4f7f\u7528alkaline incubation\uff0c\u5141\u8a31\u7d30\u80de\u88c2\u89e3(cell lysis)\u548c\u57fa\u56e0\u7d44DNA (genomic DNA)\u7684DNA\u8b8a\u6027(DNA denaturation)\uff0cDNA\u788e\u7247\u975e\u5e38\u4f4e\u3002\u9019\u5c0e\u81f4\u64f4\u589e\u7684DNA\u5177\u6709\u9ad8\u5b8c\u6574\u6027\u548c\u7247\u6bb5\u9577\u5ea6\uff0c\u56e0\u6b64\u5927\u591a\u6578\u5e8f\u5217\u5747\u52fb\u8868\u793a\u3002<\/p>\n

\"TruePrime<sup>\u00c2\u00ae<\/sup><\/p>\n

\u7279\u9ede\u548c\u512a\u9ede<\/strong>Features and Benefits:<\/strong><\/p>\n

    \n
  1. \u5305\u62ec\u7d30\u80de\u88c2\u89e3\u7de9\u885d\u6db2 Cell lysis buffer included\u00a0\u2013 (if DNA already purified select the\u00a0TruePrime\u00ae<\/sup>\u00a0WGA kit<\/a>\u00a0instead)<\/li>\n
  2. Primer-free \u65b9\u6cd5 \u2013 TthPrimPol synthesizes the primers for Phi29 DNA polymerase<\/li>\n
  3. No primer artefacts \u2013 No random extension of primer dimers<\/li>\n
  4. Insensitive to external DNA contamination<\/li>\n
  5. Works with all types of genomes<\/li>\n
  6. Reduced amplification bias in genome coverage \u2013 reliable results<\/li>\n
  7. \u975e\u5e38\u9069\u5408Next Generation Sequencing<\/li>\n<\/ol>\n

    \"Trueprime<sup>\u00c2\u00ae<\/sup><\/p>\n

    \n

    \u71b1\u9580\u5546\u54c1\u00a0400nm\u4e73\u81a0\u73e0\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (\u7d05\u8272)<\/a>\u00a0|\u00a0400nm\u4e73\u81a0\u73e0\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (\u85cd\u8272)\u00a0<\/a>|\u00a0400nm\u4e73\u81a0\u73e0\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (\u9ed1\u8272)<\/a>\u76f8\u95dc\u5546\u54c1\u00a0Streptavidin-gold\u5948\u7c73\u91d1\u7c92\u5b50(40nm)<\/a>\u00a0|\u00a0Streptavidin-gold\u5948\u7c73\u91d1\u7c92\u5b50(20nm)<\/a>\u00a0|\u00a0Biotin-gold\u5948\u7c73\u91d1\u7c92\u5b50(40nm)<\/a>\u00a0|\u00a010-80nm\u5948\u7c73\u91d1\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44<\/a>\u00a0|\u00a0\u5948\u7c73\u91d1\u6eb6\u6db2<\/a><\/span><\/p>\n

    \u5f15\u7528\u6587\u737b<\/p>\n

    Picher, A. J. et al. TruePrime\u00ae\u00a0is a novel method for whole-genome amplification from single cells based on TthPrimPol.\u00a0Nat. Commun.\u00a07, 13296 doi: 10.1038\/ncomms13296 (2016)<\/p>\n

    Circular Single-Stranded Nucleid Acid, Method for Preparing the same, and Method for using the same (FreePatentsOnline, 2018)<\/p>\n<\/div>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    TruePrime\u00ae\u55ae\u7d30\u80deWGA\u8a66\u5291\u76d2\u00a0|\u00a0\u8ca8\u865f351100 \u7522\u54c1\u4ecb\u7d39:\u00a0TruePrime\u00ae\u55ae\u7d30\u80deWGA\u8a66\u5291 […]<\/p>\n","protected":false},"author":3,"featured_media":21682,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1965],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21690"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21690"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21690\/revisions"}],"predecessor-version":[{"id":21694,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21690\/revisions\/21694"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/21682"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21690"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21690"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21690"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}