{"id":21660,"date":"2019-08-19T15:29:25","date_gmt":"2019-08-19T07:29:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21660"},"modified":"2022-07-20T10:52:22","modified_gmt":"2022-07-20T02:52:22","slug":"taq-dna-ligase-%e8%b2%a8%e8%99%9f-e090","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21660","title":{"rendered":"Taq DNA Ligase | \u8ca8\u865f E090"},"content":{"rendered":"

Taq DNA Ligase<\/strong> | <\/strong>\u8ca8\u865f<\/strong> E090<\/strong>\u00a0 <\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39: Taq DNA\u9023\u63a5\u9176(Taq DNA Ligase)\u662f\u71b1\u7a69\u5b9a\u9023\u63a5\u9176(thermostable ligase)\uff0c\u5176\u50ac\u5316\u96d9\u93c8DNA\u7684\u76f8\u91305′-\u78f7\u9178\u548c3′-\u7fa5\u57fa(3\u2019-hydroxyl groups)\u4e4b\u9593\u7684\u78f7\u9178\u4e8c\u916f\u9375\u7684\u5f62\u6210\u3002 \u8a72\u9176\u662fNAD +\u4f9d\u8cf4\u6027DNA\u9023\u63a5\u9176(NAD+ dependent DNA ligase)\u3002\u4e00\u500b\u55ae\u4f4d\u5b9a\u7fa9\u70ba\u572845\u2103\u4e0b15\u5206\u9418\u5167\u572850\u03bcl\u7684\u7e3d\u53cd\u61c9\u9ad4\u7a4d\u4e2d1\u03bcg BstEII\u6d88\u5316\u7684DNA\u768412bp\u7c98\u6027\u672b\u7aef50\uff05\u9023\u63a5\u7684\u91cf\u3002<\/p>\n

\"\"<\/p>\n

Nick-selective ligation activity means there is no end-joining of DNA fragments.<\/p>\n

High-fidelity ligation \u2013 no ligation of nicks containing base pair mismatches.<\/p>\n

SNP detection using Ligase Chain Reaction and Ligase Detection Reaction.<\/p>\n

Mutagenesis by incorporation of a mutagenic oligonucletide that has been phosphorylated.<\/p>\n

\u5176\u5b83<\/strong>\u5546\u54c1\u00a0<\/strong>AKT2<\/u><\/strong>\u00a0<\/u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/a> | <\/strong>EGFR<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>HER2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>LIMK1<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>MEK1 (EE)<\/u><\/strong>\u00a0<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>\u00a0<\/u><\/strong>ROCK2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>ROS1 (G2032R)<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nTaq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between adjacent 5\u2019-phosphate and 3\u2019-hydroxyl groups of duplex DNA. This enzyme is a NAD+ dependent DNA ligase.<\/td>\n<\/tr>\n
Application<\/strong><\/td>\n\n

\u00b7\u00a0 Nick-selective ligation activity means there is no end-joining of DNA fragments.<\/p>\n

\u00b7\u00a0 High-fidelity ligation \u2013 no ligation of nicks containing base pair mismatches.<\/p>\n

\u00b7\u00a0 SNP detection using Ligase Chain Reaction and Ligase Detection Reaction.<\/p>\n

\u00b7\u00a0 Mutagenesis by incorporation of a mutagenic oligonucletide that has been phosphorylated.<\/td>\n<\/tr>\n

Form<\/strong><\/td>\nEnzyme supplied with a 10X Reaction Buffer.
\nIncubate at 45\u00b0C.<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n40000 U\/ml<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nStore all components at -20\u00b0C upon arrival. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. For long term storage, store the enzyme at -20\u00b0C, and the reaction buffer at -80\u00b0C to extend the half life of NAD+.<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol.<\/td>\n<\/tr>\n
Notes<\/strong><\/td>\n\n

One unit is defined as the amount of enzyme required to give 50% ligation of the 12 bp cohesive ends of 1 \u00b5g of BstEII-digested \u03bb DNA in a total reaction volume of 50 \u00b5l in 15 min at 45\u00b0C.<\/p>\n

Taq DNA Ligase cannot be used as a substitute for T4 DNA Ligase.<\/p>\n

This product is distributed for laboratory research only.
\nCaution: Not for diagnostic use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Taq DNA Ligase | \u8ca8\u865f E090\u00a0 \u7522\u54c1\u4ecb\u7d39: Taq DNA\u9023\u63a5\u9176(Taq DNA Liga […]<\/p>\n","protected":false},"author":3,"featured_media":21661,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1969],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21660"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21660"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21660\/revisions"}],"predecessor-version":[{"id":50750,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21660\/revisions\/50750"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/21661"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21660"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21660"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21660"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}