{"id":21623,"date":"2019-08-08T17:58:59","date_gmt":"2019-08-08T09:58:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21623"},"modified":"2022-07-20T10:53:38","modified_gmt":"2022-07-20T02:53:38","slug":"gfp-tag%e5%a4%9a%e6%a0%aa%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9fg095","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21623","title":{"rendered":"GFP Tag\u591a\u682a\u6297\u9ad4 |polyclonal anti-GFP antibody \u8ca8\u865fG095"},"content":{"rendered":"

GFP Tag\u591a\u682a\u6297\u9ad4 | \u8ca8\u865fG095, 100ug<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39: GFP Tag\u591a\u682a\u6297\u9ad4\uff0c\u514d\u75ab\u539f\u70ba\u514d\u75ab\u539f\u662f\u7da0\u8272\u87a2\u5149\u86cb\u767d\uff08Green Fluorescent Protein , GFP\uff09\u878d\u5408\u86cb\u767d\uff0c\u4f86\u81ea\u7dad\u591a\u5229\u4e9e\u6c34\u6bcd\uff08Aequorea victoria\uff09\u7684\u5168\u9577\u6c28\u57fa\u9178\u5e8f\u5217\uff08246aa\uff09\u3002\u6b64\u6297\u9ad4\u901a\u904e\u514d\u75ab\u89aa\u548c\u5c64\u6790\uff0c\u4f7f\u7528\u8207\u74ca\u8102\u7cd6\u73e0\u5076\u806f\u7684\u7da0\u8272\u87a2\u5149\u86cb\u767d\uff08Aequorea victoria\uff09\uff0c\u7136\u5f8c\u9032\u884c\u56fa\u76f8\u5438\u9644\uff0c\u5f9e\u55ae\u7279\u7570\u6027\u6297\u8840\u6e05\u88fd\u5099\u8a72\u7522\u7269\uff0c\u4ee5\u9664\u53bb\u4efb\u4f55\u4e0d\u9700\u8981\u7684\u53cd\u61c9\u6027\u3002\u6297\u9ad4\u7d14\u5316\u5f8c\uff0c\u4fdd\u5b58\u65bcPBS, pH 7.4 with 0.05% sodium azide\u3002\u6297\u9ad4\u6fc3\u5ea6\u70ba100\u00b5g\/100ul\uff0c\u6700\u4f73\u5be6\u9a57\u6fc3\u5ea6\u8acb\u53c3\u8003\u64cd\u4f5c\u624b\u518a\u3002<\/p>\n

\"Functionality<\/p>\n

\u6297\u9ad4\u4e4b\u4fdd\u5b58<\/strong>:<\/strong> \u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\uff1b4\u00b0C\u4fdd\u5b58\uff1b\u82e5\u9700\u9577\u671f\u4fdd\u5b58\u8acb\u8caf\u5b58\u65bc-20\u00b0C\u3002\u53ef\u61c9\u7528\u65bcWB, IHC, IP, ELISA, IF\u5be6\u9a57\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/strong><\/td>\nAnti-GFP Tag Antibody (Goat Polyclonal)<\/td>\n<\/tr>\n
Subtype<\/strong><\/td>\ntag<\/td>\n<\/tr>\n
Application<\/strong><\/td>\nWB, IHC, IP, ELISA, IF<\/td>\n<\/tr>\n
Recommended Dilution<\/strong><\/td>\nELISA 1:40,000; WESTERN BLOT 1:400 – 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 – 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200<\/td>\n<\/tr>\n
Raised In<\/strong><\/td>\ngoat<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nRecognize GFP and its variants.<\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nThe anti-GFP immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.<\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Formulation<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Quantity<\/strong><\/td>\n100 \u03bcg<\/td>\n<\/tr>\n
Clone<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nThis product is stable for several weeks at 4\u00b0C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20\u00b0C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\nPBS, pH 7.4 with 0.05% sodium azide.<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n100\u00b5g\/100ul<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\nThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nIgG<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nother<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Sutton, LM\u00a0et al<\/em>. “Hip14l-deficient mice develop neuropathological and behavioural features of Huntington disease.” Hum Mol Genet 22 (3):452-465 (2013).\u00a0DOI: 10.1093\/hmg\/dds441<\/a>. Application: Immunoprecipitation.<\/p>\n

Luchniak, A\u00a0et al<\/em>. “Structure-function analysis of yeast.” Methods Cell Biol 115:355-374 (2013).\u00a0DOI: 10.1016\/B978-0-12-407757-7.00022-0<\/a>. Application: SDS-PAGE.<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

GFP Tag\u591a\u682a\u6297\u9ad4 | \u8ca8\u865fG095, 100ug \u7522\u54c1\u4ecb\u7d39: GFP Tag\u591a\u682a\u6297\u9ad4\uff0c\u514d\u75ab\u539f\u70ba\u514d\u75ab\u539f\u662f\u7da0 […]<\/p>\n","protected":false},"author":3,"featured_media":21624,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[1978],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21623"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21623"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21623\/revisions"}],"predecessor-version":[{"id":21627,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21623\/revisions\/21627"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/21624"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21623"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21623"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21623"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}