{"id":21613,"date":"2019-08-08T16:06:48","date_gmt":"2019-08-08T08:06:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21613"},"modified":"2022-07-20T10:53:52","modified_gmt":"2022-07-20T02:53:52","slug":"sdh%e5%b1%b1%e6%a2%a8%e7%b3%96%e9%86%87%e8%84%ab%e6%b0%ab%e9%85%b6%e6%aa%a2%e6%b8%ac-sorbitol-dehydrogenase-activity-assay-kit-%e8%b2%a8%e8%99%9fk935","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21613","title":{"rendered":"SDH\u5c71\u68a8\u7cd6\u9187\u812b\u6c2b\u9176\u6aa2\u6e2c | Sorbitol Dehydrogenase Activity Assay Kit \u8ca8\u865fK935"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=21613\">SDH\u5c71\u68a8\u7cd6\u9187\u812b\u6c2b\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0 |\u00a0<strong>\u8ca8\u865f<\/strong>K935<\/a><\/span><\/p>\n<p>Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric)<\/p>\n<p><strong>\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u5c71\u68a8\u7cd6\u9187\u812b\u6c2b\u9176\uff08Sorbitol Dehydrogenase , SDH\uff09\uff0c\u4e5f\u7a31\u70baL-\u827e\u675c\u7cd6\u9187\u812b\u6c2b\u9176\uff08EC 1.1.1.14\uff09\u53c3\u8207\u591a\u5143\u9187(polyol pathway)\u9014\u5f91\u3002\u4e5f\u7a31\u70ba\u5c71\u68a8\u7cd6\u9187 &#8211; \u919b\u7cd6\u9014\u5f91(sorbitol-aldose pathway)\uff0c\u8a72\u9014\u5f91\u5206\u70ba\u5169\u500b\u6b65\u9a5f\uff1a\u9996\u5148\uff0c\u8461\u8404\u7cd6\u901a\u904e\u919b\u7cd6\u9084\u539f\u9176\uff08L-iditol dehydrogenase ,AR\uff09\u7684\u50ac\u5316\u4f5c\u7528\u9084\u539f\u70ba\u5c71\u68a8\u7cd6\u9187(sorbitol)\uff0c\u800c\u7b2c\u4e8c\u6b65\u7531\u5c71\u68a8\u7cd6\u9187\u812b\u6c2b\u9176(sorbitol dehydrogenase)\u50ac\u5316\uff0c\u5c71\u68a8\u9187\u812b\u6c2b\u9176(sorbitol dehydrogenase)\u5229\u7528\u5c71\u68a8\u7cd6\u9187(sorbitol)\u4f5c\u70ba\u5e95\u7269\u7136\u5f8c\u7522\u751f\u679c\u7cd6(fructose)\u3002\u5c71\u68a8\u7cd6\u9187(sorbitol)\u4e0d\u662f\u7d30\u80de\u819c\u53ef\u6ef2\u900f\u7684\u5206\u5b50;\u800c\u679c\u7cd6(fructose)\u53ef\u4ee5\u5728\u7d30\u80de\u5167\u9032\u4e00\u6b65\u4ee3\u8b1d\u3002\u6700\u8fd1\u7684\u7814\u7a76\u767c\u73fe\u5c71\u68a8\u9187\u812b\u6c2b\u9176(sorbitol dehydrogenase)\u7684\u7f3a\u4e4f\u5c0e\u81f4\u5c71\u68a8\u7cd6\u9187(sorbitol)\u7684\u7d30\u80de\u5167\u7a4d\u7d2f\uff0c\u9019\u5c0e\u81f4\u7cd6\u5c3f\u75c5\u7684\u4e26\u767c\u75c7\u3002\u8a72\u9176\u4e3b\u8981\u5728\u809d\u7d30\u80de\u4e2d\u8868\u9054\uff0c\u4e26\u4e14\u50c5\u5728\u6025\u6027\u809d\u640d\u50b7\u5f8c\u91cb\u653e\u5230\u8840\u6d41\u4e2d\u3002\u56e0\u6b64\uff0c\u8840\u6e05\u4e2d\u5b58\u5728\u986f\u8457\u7684SDH\u6d3b\u6027\u662f\u809d\u640d\u50b7\u6216\u75be\u75c5\u7684\u6f5b\u5728\u6307\u6a19\u3002 BioVision\u7684SDH\u6aa2\u6e2c\u8a66\u5291\u76d2\u70ba\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684SDH\u6d3b\u6027\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u7c21\u4fbf\u7684\u65b9\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u5c71\u68a8\u7cd6\u9187\u812b\u6c2b\u9176(sorbitol dehydrogenase)\u5229\u7528\u63d0\u4f9b\u7684\u5e95\u7269\uff0c\u540c\u6642\u9084\u539fNAD +\u4ee5\u5f62\u6210NADH\u3002 NADH\u8207\u986f\u5f71\u5291\u53cd\u61c9\uff0c\u5c0e\u81f4\u5728OD 450nm\u8655\u5f62\u6210\u5177\u6709\u5f37\u5438\u5149\u5ea6\u7684\u9aee\u8272\u5718\u3002\u8a72\u6e2c\u5b9a\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u53ef\u4ee5\u6aa2\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u4f4e\u65bc50\u03bcU\u7684\u5c71\u68a8\u9187\u812b\u6c2b\u9176(sorbitol dehydrogenase)\u6d3b\u6027\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/9\/k935-1.jpg\" alt=\"\" width=\"272\" height=\"266\" \/><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/9\/k935-3.jpg\" alt=\"\" width=\"292\" height=\"269\" \/><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/9\/k935-2.jpg\" alt=\"\" width=\"275\" height=\"275\" \/><\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K935-100<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Summary<\/strong><\/td>\n<td>\u2022 Detection method- Absorbance (450 nm)<br \/>\n\u2022 Applications- &#8211; Measurement of sorbitol dehydrogenase (SDH) activity in various tissues\/cells<br \/>\n&#8211; \u2022 Analysis of polyol-pathways<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Absorbance (450 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Eukaryotes<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>\u2022 Measurement of Sorbitol Dehydrogenase Activity in various tissues\/cells. \u2022 Analysis of polyol-pathway.<br \/>\n\u2022 Studies of chemically-induced hepatotoxicity and acute liver injury.<br \/>\n\u2022 Freshly obtained serum from animals with suspected liver damage<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Simple, rapid &amp; convenient<br \/>\n\u2022 Can measure SDH activity as low as 50 \u00b5U in a variety of samples<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 SDH Assay Buffer<br \/>\n\u2022 SDH Substrate<br \/>\n\u2022 SDH Developer<br \/>\n\u2022 NADH Standard<br \/>\n\u2022 SDH Positive Control<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage Conditions<\/strong><\/td>\n<td>-20\u00b0C<\/td>\n<\/tr>\n<tr>\n<td><strong>Shipping Conditions<\/strong><\/td>\n<td>Gel Pack<\/td>\n<\/tr>\n<tr>\n<td><strong>USAGE<\/strong><\/td>\n<td>For Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><a href=\"http:\/\/biopioneer.com.tw\/?p=11354\"><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2017\/09\/tag6-e1505358740644-300x106.jpg\" alt=\"\u76f8\u95dc\u8a66\u5291\" width=\"87\" height=\"36\" \/><\/a><\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=21611\">Sorbitol\u5c71\u68a8\u7cd6\u9187\u6aa2\u6e2c<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=21613\">SDH\u5c71\u68a8\u7cd6\u9187\u812b\u6c2b\u9176\u6aa2\u6e2c<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=21615\">SORD\u91cd\u7d44\u86cb\u767d<\/a><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>SDH\u5c71\u68a8\u7cd6\u9187\u812b\u6c2b\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0 |\u00a0\u8ca8\u865fK935 Sorbitol Dehydrogenase Activi [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1980],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21613"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21613"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21613\/revisions"}],"predecessor-version":[{"id":21619,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21613\/revisions\/21619"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21613"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21613"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21613"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}