{"id":21599,"date":"2019-08-07T10:08:14","date_gmt":"2019-08-07T02:08:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21599"},"modified":"2022-07-20T10:58:54","modified_gmt":"2022-07-20T02:58:54","slug":"%e8%86%9c%e8%81%af%e8%9b%8b%e7%99%bdv-pe%e7%b4%b0%e8%83%9e%e5%87%8b%e4%ba%a1%e6%aa%a2%e6%b8%ac-annexin-v-pe-apoptosis-detection-kit-plus-%e8%b2%a8%e8%99%9fk203","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21599","title":{"rendered":"\u819c\u806f\u86cb\u767dV-PE\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c | Annexin V-PE Apoptosis Detection Kit Plus \u8ca8\u865fK203"},"content":{"rendered":"

\u819c\u806f\u86cb\u767dV-PE\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c | <\/strong>\u8ca8\u865f<\/strong>K203<\/a><\/span><\/p>\n

Annexin V-PE Apoptosis Detection Kit Plus\u00a0<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u819c\u806f\u86cb\u767dV-PE\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2Plus(Annexin V-PE Apoptosis Detection Kit Plus, K203) \u53ef\u61c9\u7528\u65bc\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\u89c0\u5bdf (initiating apoptosis)\u3002\u5728\u958b\u59cb\u51cb\u4ea1\u5f8c\u4e0d\u4e45\uff0c\u5927\u591a\u6578\u7d30\u80de\u985e\u578b\u5c07\u819c\u78f7\u8102\u78f7\u8102\u9170\u7d72\u6c28\u9178\uff08phosphatidylserine , PS\uff09\u5f9e\u8cea\u819c(plasma membrane)\u5167\u8868\u9762\u8f49\u79fb\u81f3\u7d30\u80de\u8868\u9762(cell surface)\u3002\u4e00\u65e6\u9032\u5165\u7d30\u80de\u8868\u9762(cell surface)\uff0c\u901a\u904e\u7528\u819c\u806f\u86cb\u767dV(Annexin V- fluorescent)\u7684\u87a2\u5149\u7db4\u5408\u7269\u67d3\u8272\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u6aa2\u6e2cPS\uff0c\u819c\u806f\u86cb\u767dV (Annexin V)\u662f\u4e00\u7a2e\u5c0dPS\u5177\u6709\u5f37\u70c8\u5929\u7136\u89aa\u548c\u529b\u7684\u86cb\u767d\u8cea\u3002\u4e00\u6b65\u67d3\u8272\u7a0b\u5e8f\u50c5\u970010\u5206\u9418\u3002\u6b64\u5916\uff0c\u8a72\u6e2c\u5b9a\u53ef\u76f4\u63a5\u5728\u6d3b\u7d30\u80de\u4e0a\u9032\u884c\uff0c\u7121\u9700\u56fa\u5b9a\u3002Annexin V-PE\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2Plus\u5305\u62ec\u819c\u806f\u86cb\u767dV-PE (annexin V-PE)\uff0cSYTOX\u7da0\u8272\u67d3\u6599(SYTOX Green dye)\u548c\u7d50\u5408\u7de9\u885d\u6db2(binding buffer)\u3002 SYTOX\u7da0\u8272\u67d3\u6599(SYTOX green dye)\u5c0d\u6d3b\u7d30\u80de\u548c\u51cb\u4ea1\u7d30\u80de\u4e0d\u53ef\u6ef2\u900f\uff0c\u4f46\u901a\u904e\u8207\u7d30\u80de\u6838\u9178(cellular nucleic acids)\u7d50\u5408\u800c\u67d3\u8272\u58de\u6b7b\u7d30\u80de(necrotic cells)\uff0c\u5177\u6709\u5f37\u70c8\u7684\u7da0\u8272\u87a2\u5149\u3002\u5728\u63d0\u4f9b\u7684\u7d50\u5408\u7de9\u885d\u6db2\u4e2d\u7528\u819c\u806f\u86cb\u767dV-PE\u548cSYTOX Green\u67d3\u6599\u67d3\u8272\u7d30\u80de\u7fa4\u5f8c\uff0c\u51cb\u4ea1\u7d30\u80de\u986f\u793a\u7d05\u8272\u87a2\u5149\uff0c\u6b7b\u7d30\u80de\u986f\u793a\u7da0\u8272\u87a2\u5149\u548c\uff0c\u6d3b\u7d30\u80de\u986f\u793a\u5f88\u5c11\u6216\u6c92\u6709\u87a2\u5149\u3002\u9019\u4e9b\u7fa4\u9ad4\u53ef\u901a\u904e\u87a2\u5149\u986f\u5fae\u93e1\u4f7f\u7528FITC\u548cPE filters\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u4f7f\u7528FL1 channel\uff08Ex.488 nm \/ Em.530 nm\uff09\u7528\u65bcSYTOX Green\u67d3\u6599\u548cFL2\u901a\u9053\u7528\u65bcAnnexin<\/em> V-PE\uff08Ex.488 nm\uff09\u8f15\u9b06\u5340\u5206\/ Em.578nm\uff09\u3002<\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0|\u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668<\/strong> Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n
Detection Method<\/strong><\/td>\nFlow cytometry using FL1 channel for SYTOX Green dye (Ex = 488 nm; Em = 530 nm) and FL2 channel for Annexin V-PE (Ex = 488 nm; Em = 578 nm).<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetecting apoptosis in living cells by flow cytometry or fluorescence microscopy. PE shows brighter red fluorescence. Sytox green dye is also included to control necrosis.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple one-step procedure; takes only 10 minutes
\n\u2022 Fast and convenient
\n\u2022 Apoptotic cells show red fluorescence, dead cells show green fluorescence and \u2022 Live cells show little or no fluorescence.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Annexin V-PE
\n\u2022 SYTOX Green Dye
\n\u2022 Binding Buffer<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n4\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Epstein Shochet, et. al. Hormone-dependent placental manipulation of breast cancer cell migration.\u00a0Hum. Reprod.,<\/a>Nov 2011; 10.1093\/humrep\/der365.<\/p>\n

Horton HM et al (2008) Cancer Res. 68: 8049 – 8057.<\/p>\n

Snow A.L. et al.(2006) J. Immunol. 177: 3283-3293.<\/p>\n

Tiwari M.M. et al. (2006) Toxicol. Sci. 91: 493-500.<\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u819c\u806f\u86cb\u767dV-PE\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c | \u8ca8\u865fK203 Annexin V-PE Apoptosis Detectio […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[190],"tags":[1984],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21599"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21599"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21599\/revisions"}],"predecessor-version":[{"id":21600,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21599\/revisions\/21600"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21599"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21599"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21599"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}