{"id":21524,"date":"2019-07-29T18:08:54","date_gmt":"2019-07-29T10:08:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21524"},"modified":"2022-07-20T11:02:48","modified_gmt":"2022-07-20T03:02:48","slug":"%e8%91%a1%e8%90%84%e7%b3%96%e6%bf%80%e9%85%b6%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a-%e8%b2%a8%e8%99%9fk969-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21524","title":{"rendered":"\u8461\u8404\u7cd6\u6fc0\u9176\u6d3b\u6027\u6e2c\u5b9a | \u8ca8\u865fK969-100"},"content":{"rendered":"

\u8461\u8404\u7cd6\u6fc0\u9176\u6d3b\u6027\u6e2c\u5b9a\u5957\u7d44 | <\/strong>\u8ca8\u865f<\/strong>K969-100<\/a><\/span><\/p>\n

PicoProbe\u2122 Glucokinase Activity Assay Kit (Fluorometric)<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>Glucokinase\u8461\u8404\u7cd6\u6fc0\u9176\uff08\u4e5f\u7a31\u70baGCK\uff0c\u5df1\u7cd6\u6fc0\u9176IV\u578b\u6216D\u548cATP\uff1aD-\u5df1\u7cd66-\u78f7\u9178\u8f49\u79fb\u9176; EC 2.7.1.1\uff09\u5728\u7279\u5b9a\u985e\u578b\u7684\u7d44\u7e54\u4e2d\u8868\u9054\uff1a\u809d\u81df(liver)\uff0c\u80f0\u817a(pancreas)\uff0c\u5c0f\u8178(small intestine)\u548c\u8166(brain)\u3002\u8461\u8404\u7cd6\u6fc0\u9176(Glucokinase)\u4f5c\u70ba\u8461\u8404\u7cd6\u50b3\u611f\u5668(glucose sensor)\u8d77\u4f5c\u7528\uff0c\u53cd\u61c9\u65bc\u8840\u6db2\u4e2d\u8461\u8404\u7cd6\u7684\u6c34\u5e73\uff0c\u4f8b\u5982\u71df\u990a(nutritional)\u548c\u6fc0\u7d20\u5206\u5b50\u9014\u5f91(hormonal molecular pathways)\uff0c\u5f15\u767c\u78b3\u6c34\u5316\u5408\u7269\u4ee3\u8b1d(hyperglycemia)\u6216\u7d30\u80de\u529f\u80fd\u7684\u8b8a\u5316\u3002\u8207\u5176\u4ed6\u5df1\u7cd6\u6fc0\u9176(Hexokinases)\u4e0d\u540c\uff0c\u8461\u8404\u7cd6\u6fc0\u9176\u5c0d\u8461\u8404\u7cd6\u7684\u89aa\u548c\u529b\u76f8\u5c0d\u8f03\u4f4e\uff0c\u4e26\u4e14\u4e0d\u53d7\u751f\u7406\u6fc3\u5ea6\u7684\u8461\u8404\u7cd6-6-\u78f7\u9178\u7684\u6291\u5236\u3002gene encoding GCK\u7684\u57fa\u56e0\u7a81\u8b8a\u53ef\u5f15\u8d77\u9ad8\u8840\u7cd6\u75c7(hyperglycemia)\u548c\u4f4e\u8840\u7cd6\u75c7(hypoglycemia)\u3002\u7531\u65bc\u8461\u8404\u7cd6\u6fc0\u9176(Glucokinase)\u5728\u63a7\u5236\u8840\u7cd6\u7a69\u614b\u4e2d\u5177\u4e3b\u8981\u4f5c\u7528\uff0c\u8461\u8404\u7cd6\u6fc0\u9176(Glucokinase)\u76ee\u524d\u88ab\u8a8d\u70ba\u662f\u6cbb\u7642\u9ad8\u8840\u7cd6\u75c7\u5f37\u6709\u529b\u5019\u9078\u9776\u6a19\uff0c\u9ad8\u8840\u7cd6\u75c7\u662f2\u578b\u7cd6\u5c3f\u75c5(Type 2 Diabetic patients)\u60a3\u8005\u4e2d\u9047\u5230\u7684\u75c5\u75c7\u3002BioVision\u7684PicoProbe\u2122\u8461\u8404\u7cd6\u6fc0\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 (PicoProbe\u2122 Glucokinase Activity Assay Kit, K816-6-100)\u3002\u70ba\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684GCK\u6d3b\u6027\u76e3\u6e2c\uff0c\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u7c21\u4fbf\u7684\u65b9\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cGCK\u5c07\u8461\u8404\u7cd6\u8f49\u5316\u70ba\u8461\u8404\u7cd6-6-\u78f7\u9178(glucose-6-phosphate)\uff0c\u5176\u53c8\u8f49\u5316\u70ba\u4e00\u7cfb\u5217\u4e2d\u9593\u9ad4\uff0c\u5176\u6e1b\u5c11PicoProbe TM<\/sup>\uff0c\u7522\u751f\u5f37\u87a2\u5149\u7522\u7269\uff08Ex \/ Em = 535 \/ 587nm\uff09\u3002\u8a72\u6e2c\u5b9a\u6cd5\u7c21\u55ae\uff0c\u7279\u7570\uff0c\u9748\u654f\u4e14\u9069\u61c9\u9ad8\u901a\u91cf\uff0c\u53ef\u6aa2\u6e2c\u4f4e\u81f32\u03bcU\u7684GCK\u6d3b\u6027\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK969-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Fluorescence (Ex\/Em = 535\/587 nm)
\n\u2022 Applications-Measurement of Glucokinase activity in various tissues\/cells.
\n\u2022 Analysis of Glucose metabolism and Cell signaling in various cell types.
\n\u2022 Screening anti-diabetic drugs.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em = 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Measurement of Glucokinase activity in various tissues\/cells.
\n\u2022 Analysis of Glucose metabolism and Cell signaling in various cell types.
\n\u2022 Screening anti-diabetic drugs.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple and rapid procedure
\n\u2022 Highly Sensitive
\n\u2022 Can detect glucokinase activity even as low as 2 \u00b5U<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 GCK Assay Buffer
\n\u2022 PicoProbe\u2122 (in DMSO)
\n\u2022 DTT (1 M)
\n\u2022 GCK Substrate
\n\u2022 Sample Background Reagent
\n\u2022 ATP
\n\u2022 GCK Enzyme Mix
\n\u2022 GCK Developer
\n\u2022 GCK Positive Control
\n\u2022 NAPDH Standard (200 nmol)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u8461\u8404\u7cd6\u6fc0\u9176\u6d3b\u6027\u6e2c\u5b9a\u5957\u7d44 | \u8ca8\u865fK969-100 PicoProbe\u2122 Glucokinase Activit […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2014],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21524"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21524"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21524\/revisions"}],"predecessor-version":[{"id":21525,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21524\/revisions\/21525"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21524"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21524"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21524"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}