{"id":21386,"date":"2019-07-18T15:24:43","date_gmt":"2019-07-18T07:24:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21386"},"modified":"2022-07-20T11:07:02","modified_gmt":"2022-07-20T03:07:02","slug":"rabbit-anti-goat-igghl-human-sp-ads-hrp%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f6164-05","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=21386","title":{"rendered":"Rabbit Anti-Goat IgG(H+L), Human SP ads-HRP\u6297\u9ad4 | \u8ca8\u865f6164-05"},"content":{"rendered":"

Rabbit Anti-Goat IgG(H+L), Human SP ads-HRP\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong><\/a><\/span>6164-05\u00a0\u00a0<\/a><\/span> \u00a0\u00a0<\/strong>\u898f\u683c<\/strong>1.0 mL<\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

\u4e8c\u7d1a\u6297\u9ad4 (Secondary antibodies) \u61c9\u7528:<\/p>\n

\u4e8c\u6297(Secondary antibodies)\u662f\u91dd\u5c0d\u514d\u75ab\u7403\u86cb\u767d\uff08immunoglobulin , Ig\uff09\u5206\u5b50\u7684\u591a\u514b\u9686(polyclonal) \u548c\u55ae\u514b\u9686\u6297\u9ad4(monoclonal antibodies)\u3002\u5b83\u5011\u901a\u5e38\u8207\u6297\u539f\u7279\u7570\u6027\u4e00\u6297(specific primary antibody) \u4e00\u8d77\u4f7f\u7528\uff0c\u4e26\u4e14\u901a\u5e38\u88ab\u6a19\u5b9a\u9175\u7d20\u6216\u87a2\u5149\u7269\u8cea\u4ee5\u5e6b\u52a9\u6aa2\u6e2c\u3002\u4e8c\u6297\u53ef\u7528\u65bc\u591a\u7a2e\u61c9\u7528\uff0c\u4f8b\u5982ELISA\u3001\u514d\u75ab\u5370\u8de1(Immunoblotting)\u3001\u6d41\u5f0f\u7d30\u80de\u5100(Flow Cytometry)\u3001\u514d\u75ab\u7d44\u7e54\u67d3\u8272(Immunohistochemistry)\u3001\u514d\u75ab\u7d30\u80de\u5316\u5b78(Immuocytochemistry)\u3002\u4e8c\u7d1a\u6297\u9ad4\u901a\u5e38\u7528\u9176\u6216\u87a2\u5149\u67d3\u6599\u6a19\u8a18\uff0c\u9019\u4f7f\u5f97\u80fd\u5920\u901a\u904e\u6bd4\u8272\uff0c\u5316\u5b78\u767c\u5149\u548c\u87a2\u5149\u6280\u8853\u9032\u884c\u6aa2\u6e2c\u3002\u4f8b\u5982\uff0c\u9176\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08enzymes horseradish peroxidase , HRP\uff09\u548c\u9e7c\u6027\u78f7\u9178\u9176\uff08alkaline phosphatase , AP\uff09\u901a\u904e\u6bd4\u8272\u548c\u5316\u5b78\u767c\u5149\u5e73\u53f0\u5728ELISA\u548c\u86cb\u767d\u8cea\u5370\u8de1\u61c9\u7528\u4e2d\u662f\u6709\u50f9\u503c\u7684\u3002\u87a2\u5149\u86cb\u767d\u8af8\u5982\u87a2\u5149\u7d20\uff08fluorescein , FITC\uff09\uff0c\u85fb\u7d05\u86cb\u767d\uff08phycoerythrin , PE\uff09\u548c\u82b1\u9752\uff08cyanine , CY\uff09\u7684\u87a2\u5149\u67d3\u6599\u5728\u87a2\u5149\u7684\u61c9\u7528\u4e2d\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\uff0c\u5305\u62ec\u6d41\u5f0f\u7d30\u80de\u8853\uff0c\u514d\u75ab\u87a2\u5149\u986f\u5fae\u8853\u548c\u591a\u91cd\u6e2c\u5b9a\u3002\u4e8c\u6297\u5141\u8a31\u6a19\u8a18\u548c\u6aa2\u6e2c\u7684\u9748\u6d3b\u6027\u4ee5\u53ca\u901a\u904e\u4fe1\u865f\u653e\u5927\uff0c\u53ef\u589e\u52a0\u7684\u9748\u654f\u5ea6\u3002<\/p>\n

\"\"<\/p>\n

Frozen newborn mouse rib section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01) followed by Rabbit Anti-Goat IgG(H+L), Human SP ads-FITC (SB Cat. No. 6164-02) and DAPI.<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Rabbit Anti-Goat IgG(H+L), Human SP ads-HRP\u6297\u9ad4\uff0c\u5177HRP\u6a19\u8a8c\u3002\u91dd\u5c0dgoat IgG\u8f15\u93c8\u53ca\u91cd\u93c8\u5177\u7279\u7570\u6027\u3002\u4f86\u6e90\u70baPooled antisera from rabbits hyperimmunized with goat IgG\u3002Isotype\u70baRabbit IgG\u3002\u7de9\u885d\u6db2\u914d\u65b9\u572850% Glycerol\/50% Phosphate buffered saline, pH 7.4\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Specificity<\/strong><\/td>\nReacts with the heavy and light chains of goat IgG<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nPooled antisera from rabbits hyperimmunized with goat IgG<\/td>\n<\/tr>\n
Cross Adsorption<\/strong><\/td>\nHuman serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins<\/td>\n<\/tr>\n
Purification<\/strong><\/td>\nAffinity chromatography on goat IgG covalently linked to agarose<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nRabbit IgG<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nHRP (Horseradish Peroxidase)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\n50% Glycerol\/50% Phosphate buffered saline, pH 7.4<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Quality tested applications for relevant formats include –<\/p>\n

ELISA\u00a01<\/sup>
\nFLISA<\/p>\n

Other referenced applications for relevant formats include –<\/p>\n

Flow Cytometry\u00a02-6<\/sup>
\nImmunohistochemistry-Frozen Sections\u00a07<\/sup>
\nImmunohistochemistry-Paraffin Sections\u00a08-10<\/sup>
\nImmunocytochemistry\u00a011<\/sup>
\nWestern Blot\u00a012-14<\/sup><\/td>\n<\/tr>\n

Recommended Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2796238<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/strong><\/p>\n

    \n
  1. Boxx GM, Kozel TR, Nishiya CT, Zhang MX. Influence of mannan and glucan on complement activation and C3 binding by Candida albicans. Infect Immun. 2010;78:1250-9. (ELISA)<\/li>\n
  2. Fu S, Gong F, Xie C, Zhu W, Wang W, Shen H, et al. S100A12 on circulating endothelial cells surface in children with Kawasaki disease. Pediatr Res. 2010;68:165-8. (FC)<\/li>\n
  3. Rezaee F, Rellick SL, Piedimonte G, Akers SM, O’Leary HA, Martin K, et al. Neurotrophins regulate bone marrow stromal cell IL-6 expression through the MAPK pathway. PLoS One. 2010;5(3):e9690. (FC)<\/li>\n
  4. Sekiya I, Ojima M, Suzuki S, Yamaga M, Horie M, Koga H, et al. Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis. J Orthop Res. 2012;30:943-9. (FC)<\/li>\n
  5. Gong F, Zhang Y, Xie C, Zhu W, Wang W, Fu S, et al. Expression of receptor for advanced glycation end products (RAGE) on the surface of circulating endothelial cells is upregulated in Kawasaki disease. Pediatr Res. 2012;71:720-4. (FC)<\/li>\n
  6. Hegde M, Corder A, Chow KK, Mukherjee M, Ashoori A, Kew Y, et al. Combinational targeting offsets antigen escape and enhances effector functions of adoptively transferred T cells in glioblastoma. Mol Ther. 2013;21:2087-101. (FC)<\/li>\n
  7. Kreft KL, Verbraak E, Wierenga-Wolf AF, van Meurs M, Oostra BA, Laman JD, et al. The IL-7R\u03b1 pathway is quantitatively and functionally altered in CD8 T cells in multiple sclerosis. J Immunol. 2012;188:1874-83. (IHC-FS)<\/li>\n
  8. Crijns AP, de Graeff P, Geerts D, ten Hoor KA, Hollema H, van der Sluis T, et al. MEIS and PBX homeobox proteins in ovarian cancer. Eur J Cancer. 2007;43:2495-505. (IHC-PS)<\/li>\n
  9. Ando K, Brion J, Stygelbout V, Suain V, Authelet M, Dedecker R, et al. Clathrin adaptor CALM\/PICALM is associated with neurofibrillary tangles and is cleaved in Alzheimer’s brains. Acta Neuropathol. 2013;125:861-78. (IHC-PS)<\/li>\n
  10. Ahmed L, Nalwoga H, Arnes JB, Wabinga H, Micklem DR, Akslen LA. Increased tumor cell expression of Axl is a marker of aggressive features in breast cancer among African women. APMIS. 2015 May 25. doi: 10.1111\/apm.12403. [Epub ahead of print]. (IHC-PS)<\/li>\n
  11. Hosper NA, van den Berg PP, de Rond S, Popa ER, Wilmer MJ, Masereeuw R, et al. Epithelial-to-mesenchymal transition in fibrosis: collagen type I expression is highly upregulated after EMT, but does not contribute to collagen deposition. Exp Cell Res. 2013;319:3000-9. (ICC)<\/li>\n
  12. Rayes J, Roumenina LT, Dimitrov JD, Repess\u00e9 Y, Ing M, Christophe O, et al. The interaction between factor H and VWF increases factor H cofactor activity and regulates VWF prothrombotic status. Blood. 2014;123:121-5. (WB)<\/li>\n
  13. Wu X, Sagave J, Rutkovskiy A, Haugen F, Baysa A, Nyg\u00e5rd S, et al. Expression of bone morphogenetic protein 4 and its receptors in the remodeling heart. Life Sci. 2014;97:145-54. (WB)<\/li>\n
  14. Matsebatlela TM, Anderson AL, Gallicchio VS, Elford H, Rice CD. 3,4-Dihydroxy-benzohydroxamic acid (Didox) suppresses pro-inflammatory profiles and oxidative stress in TLR4-activated RAW264.7 murine macrophages. Chem Biol Interact. 2015;233:95-105. (WB)<\/li>\n<\/ol>\n

    SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/span><\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Rabbit Anti-Goat IgG(H+L), Human SP ads-HRP\u6297\u9ad4 | \u8ca8\u865f6164- […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[2050,2049],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21386"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21386"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21386\/revisions"}],"predecessor-version":[{"id":21387,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21386\/revisions\/21387"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21386"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21386"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21386"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}